• Journal of Plant Biology
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• 제38권3호
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• pp.227-234
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• 1995

Exogenous putrescine of 0.5 mM or higher concentratons applied during a 16 h inductive dark period could elevate putrescine content in cotyledons of Pharbitis nil Choisy cv. Violet, a short-day plant, resulting in complete blocking of photoperiodic floral induction. Titers of putrescine, spermidine and spermine in the cotyledons were traced throughout a 16 h dark period. While non-induced cotyledons under continous light slightly increased levels of polyamines, induced tissue maintaiend its putrescine, spermidine and spermine levels as low as 66.4%, 60.9% and 84.9% of non-induced levels respecitvely. Endogenous polyamines kept at lower levels in the inductive dark period were found to upsurge by a night break treatment of 10 min light in the middle of the dark and consequently the inductive dark effect was canceled. Elevation of polyamine titers could also be induced by 100 $\mu$L/L ethylene treatment which completely suppressed floral induction. Compared to untreated cotyledons, ehtylene-treated tissues increased putrescine content by as much as 136.5% in 12 h and spermidine level by up to 130.1% in 8 h. Ethylene-treated cotyledons not only increased endogenous polyamine content but also liberate ethylene in the second half of the inductive dark period accumulating up to three to fourfold level supporting a hypothesis that ethylene-treated tissues are stimulated to produce ethylene which in turn accelerates polyamine biosynthesis in the tissues. It is postulated that substantially low polyamine titers in the inductive dark period would be one of the necessary factors controlling photoperiodic induction of flowering in Pharbitis nil and the inhibitory effects of night break and exogenous ethylene treatment may be atributed to their action to stimulate endogenous polyamine production.

• 한방재활의학과학회지
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• 제21권1호
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• pp.47-56
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• 2011

Objectives : This study was conducted to identify the antimicrobial anti-oxidative and tyrosinase inhibitory effect of three kinds of herbal plants, Polygonum cuspidatum, Chelidonii herba, Pharbitis nil L. naturally grown across the nation. Methods : To investigate in vitro anti-oxidative activity assay, antimicrobial effect and tyrosinase inhibitory activity, MeOH 80% extract, n-hexane, chloroform, ethyl acetate, n-butanol and water fractions of herbal plants were tested by diphenylpicrylhydrazyl(DPPH) method. Results : Bsutanol fractions of each plants showed about 90% on anti-oxidative effect. In case of tyrosinase activity, ethlyacetate fraction of Polygonum cuspidatum showed a potent inhibition effect. In the antimicrobial effect, ethylacetate fraction of Polygonum cuspidatu, hexane fractions of Chelidonii herba and the methanol 80% extracts of Pharbitis nil L. exhibited inhibition effect significantly. Conclusions : In MeOH 80% extracts, we couldn't found any extracts or fractions which have antimicrobial, anti-oxidative and tyrosinase inhibitory activity, however ethylacetate fractions of Polygonum cuspidatum showed effects commonly in these three assay system.

• 한국자원식물학회지
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• 제5권2호
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• pp.85-93
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• 1992

This experiment was Conducted ta screening for the anti-cancer efficacy from the wild plants which are naturally growing in the Korea. The results are as follows. The results were shown thatZea may L. nad significantly effects on mediculal efficacy anganist anti-tumor by usulg the totalpacked cell volume methods and also, severals plants, such as Sofonum nigrum, Patrinia hispidoBunge, Eragrostis, ferrugenia Beauv, Salaginela pouzolgiana Spring, Platrycarya strobilacea Bunge,Codonopsis lanceolata Benth. et Hook fil. which are collected from Giri and Mooju mountain in Koreaand Nagano in Japan were showed effects on auti-tumor. But the pharmaceologial activities ofPharbitis nil Choisy was believed to strong effec on anti - cancer tumors, while toxicity of its wasshown high that induced te kill all used mice. Extraction of Patrinia hispida Bunge, Pharbitis nilChoisy, Toilis japonica DC, Eragrostis erruginea Beauv. and Forsythia koreana Nakai showed effec-tively supressed on growth rate of cancer tumor by the below 50 percent of T/C ratio at 30mg /mlof extraction from plant. That Is strong activity while Reynouxria japonica Houtt. was observed onlymild activities. The above results many possibly suggest that Patrinia hispido Bunge and Eragrostisferrugina Beauv. inhibited the growth of cancer tumor by the both total packed cell volume methodand cytotoxicity method. Although basic research is still going on, we will find out an accurate moth-od for developing useful medicinal plant to improve pharmacological activites against anti-cancertumor, especialy, in Eragrostis ferruginea Beauv.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.27-39
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• 2005

a-Ketol linolenic acid [KODA, 9,10-ketol-octadecadienoic acid, or 9-hydroxy-10 -oxo-12(Z), 15(Z)-octadecadienoic acid] was found as a stress-induced factor in Lemna paucicostata. KODA reacts with catecholamines to generate many products that strongly induce flowering in L. paucicostata, although KODA itself was inactive. KODA contains an asymmetric carbon at the 9-position in the molecule; the 9-hydroxyl group is predominantly 9R, with an enantiomeric excess of 40% (70% 9R and 30% 9S). We analyzed two major products of the reaction between KODA and norepinephrine, named FN1 and FN2. FN1 was identified as a tricyclic a-ketol fatty acid, 9(R)-11-{(2'R,8’R,10'S,11'S)-2',8'-dihydroxy-7'-oxo-11'-[(Z)-2-pentenyl]-9'-oxa-4'-azatricyclo[6.3.1.01.5]dodec-5'en-10'-yl}-9-hydroxy-10-oxoundecanoic acid. FN2 was the C-9 epimer of FN1. FN1 was derived from 9R-type KODA and FN2 from 9S-type. FN1 showed strong flower-inducing activity, but FN2 was inactive. Pharbitis nil (violet) is a typical short-day plant; flowering can be induced by exposing a seedling cultivated under continuous light to a single 16-h dark period. We analyzed endogenous KODA levels and showed that they were closely related to flower induction: KODA sharply increased in the later part of a 16-h dark period, on the other hand, it failed to increase in the night-break experiment. In addition to it, KODA increased transiently in immature flower buds in all the plants we examined, including P. nil. No such increase of KODA was seen in foliar buds of P. nil. When KODA was sprayed on seedlings of Pharbitis, flower induction was promoted only by the (R)-form of KODA. We also found that KODA enhances flowering in garden plants such as carnations and impatienses. These phenomena indicate that KODA may be involved in flowering formationg of plants and it is potentially useful for a regulating agent for commercial plant flowering.

• 생명과학회지
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• 제20권8호
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• pp.1268-1275
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• 2010

본 연구에서는, 장수상황버섯(Phellinus baumii) 균사체를 이용한 한약재 고체발효 추출물의 암세포 성장억제 효과를 확인하고자 36종의 한약재 및 이들의 고체발효 한약재 추출물을 대상으로, 인간 대장암세포(HCT116) 성장억제능, 정상세포(HEK-293, 3T3-L1 및MC3T3-E1)에 대한 세포독성 및 인간 적혈구 용혈활성을 평가하였다. 선별된 36종의 한약재 추출물은 100 ${\mu}g/ml$ 농도에서, 사인, 천궁, 석곡, 백선피, 시체, 두충, 백과, 신이, 와송, 삼칠, 견우자, 원지 및 괄루인의 13종에서 암세포 성장률이 50% 이하를 나타내었으며, 균사체 추출물은 46.3%의 성장률을 나타내었다. 반면 25종의 발효한약재 추출물은 대부분 암세포 성장억제능의 변화가 미미하였으나, 백선피, 백과, 신이, 황부자, 산약, 현삼 및 괄루인은 암세포 증식억제능이 유의적으로 감소하였으며, 도인, 골쇄보, 구기자 및 패모에서는 암세포 성장억제능이 유의적으로 증가하였다. 특히 골쇄보, 구기자 및 패모 추출물의 대장암세포에 대한 $IC_{50}$는 각각 394, 917 및 149 ${\mu}g/ml$이었으나, 발효 후 각각 28, 85 및 80 ${\mu}g/ml$를 나타내었다. 또한 발효 골쇄보, 구기자 및 패모 추출물은 200 ${\mu}g/ml$ 농도까지 정상세포에서 미미한 세포독성을 나타내었다. 이러한 결과는 장수상황버섯 균사체를 이용한 한약재 고체발효가 새로운 항암 활성물질 개발 및 신규의 생리활성물질 생산에 이용될 수 있음을 제시하고 있다.