• 한국어병학회지
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• 제16권2호
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• pp.103-110
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• 2003

• 대한수의학회지
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• 제58권2호
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• pp.95-98
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• 2018

Brucellosis is one of the common zoonoses caused by Brucella abortus (B. abortus). However, little has been reported on factors affecting invasion of B. abortus into host cells. To investigate cell-type dependent invasion of B. abortus, phagocytic RAW 264.7 and THP-1 cells and non-phagocytic HeLa cells were infected with wild-type and mutant B. abortus, and their invasion efficiencies were compared. The invasion efficiencies of the strains were cell-type dependent. Wild-type B. abortus invasion efficiency was greater in phagocytic cells than in epithelial cells. The results also indicated that there are different factors involved in the invasion of B. abortus into phagocytic cells.

• Fisheries and Aquatic Sciences
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• 제3권2호
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• pp.78-82
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• 2000

The influence of daily water temperature changes on chemiluminescent (CL) response of phagocytes, plasma glucose level and mortality in cultured gray mullet (Mugil cephalus) was investigated. Among three experimental temperature groups, the fish in group I and group II were maintained constantly at $27^{\circ}C$ and $29^{\circ}C$, respectively, and those in group III were suffered daily elevation of water temperature from $27^{\circ}C$ to $29^{\circ}C$ for 5 hours, then restored again to $27^{\circ}C$. After 1 week of maintaining at each experimental tank, the fish in group III showed significantly lower CL peak value (p < 0.01) and higher plasma glucose levels (p < 0.05) than those in group I and group II. The cumulative mortality of group III was $86.9\%$ within 3 days after being subjected to acute handling stress. On the other hand, the cumulative mortalities of group I and group II were $12.5\%$ and $19.2\%$, respectively. In conclusion, gray mullet farms, especially, in the vicinity of thermoelectric power plants should avoid stressing the fish during periods of high water temperature.

• 한국수산과학회지
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• 제37권4호
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• pp.275-280
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• 2004

In this study, the effcts of kelp (Ascophyllum nodosum) meal on growth and immune responses of parrot fish (Oplegnathus fasciatus) were studied. Fish were fed an experimental diet supplemented with $2{\%}\;and\;5{\%}$ kelp meal in a controled diet. Several factors such as weight gain, hematological parameters and nonspecific immune responses were evaluated far 0, 2, 4 and 8 weeks after the administration of the kelp meal supplemented diet. Weight gain in the fish fed the diet supplemented with $2{\%}\;and\;5{\%}$ kelp meal was not significant among the tested groups. The NBT reaction of the phagocytes in the head kidney and the phagocytic rate/index of phagocytes in the peripheral blood were significantly higher than the control group. But, there was no significant difference both in complement bactericidal activity, mucus Iysozyme activity and hematology among each group.

• 한국어병학회지
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• 제12권1호
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• pp.1-6
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• 1999

알로에가 in vitro에서 넙치의 비특이적 면역반응에 미치는 영향을 조사하기 위하여 신장으로부터 분리된 백혈구의 호흡폭발을 chemiluminescent(CL) 반응을 이용하여 측정하였다. 알로에 분말을 0.001, 0.01, 0.1, 0.5, 1.0, $10.0{\mu}g/ml$의 농도로 MEM에 희석하여 넙치의 식세포와 함께 배양한 후 3, 6, 12, 24, 48시간째에 CL 반응을 이용하여 호흡폭발능을 측정한 결과, 알로에 $0.5{\mu}g/ml$과 $1.0{\mu}g/ml$의 농도에서 24시간 동안 배양한 실험구만이 알로에를 첨가하지 않은 대조구에 비해서 유의성 있게 호흡폭발능이 증가하였다. 이러한 결과는 알로에가 양식 넙치의 비특이적 면역능을 증강시킬 수 있음을 시사하며, 앞으로 in vivo 실험을 통한 검증이 필요할 것으로 사료된다.

• 한국어병학회지
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• 제13권2호
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• pp.137-142
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• 2000

간흡충 탈낭유충에 대한 잉어, 붕어, 참붕어의 보체 살충능 및 식세포 chemiluminescent 반응 차이를 조사하였다. 낙동강에서 채집한 참붕어는 모두 간흡충 피낭유충에 중감염되어 있었으나, 진영의 저수지에서 채집한 참붕어 및 낙동강에서 채집한 붕어와 양식 잉어에서는 간흡충 피낭유충이 전혀 검출되지 않았다. 각 실험어의 신선혈청을 이용하여 간흡충 탈낭유충에 대한 살충능을 조사한 결과 간흡충 피낭유충에 중감염된 참붕어 혈청과 붕어의 혈청은 잉어의 혈청에 비해 높은 살충능을 나타낸 반면, 간흡충 피낭유충에 감염되지 않은 참붕어의 혈청은 가장 낮은 살충능을 나타냈다. 각 어종의 혈청을 $47^{\circ}C$에서 30분간 처리하여 보체를 불활화시킨 결과 모든 실험어종에서 혈청의 살충능이 완전히 사라졌다. 간흡충 탈낭유충 균질액의 상층액을 각 어종의 두신 식세포에 첨가하여 chemiluminescent(CL) 반응을 측정한 결과 참붕어와 잉어에서 CL반응이 zymosan만을 첨가했을때에 비해 유의적(P<0.05)으로 감소하였으며, 각 어종별 간흡충 탈낭유충 균질액의 상충액 첨가에 의한 CL반응 감소율은 참붕어의 경우 22.9%, 붕어 9.6%,잉어 12.4%로 나타났다.

• 한국임상수의학회:학술대회논문집
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• 한국임상수의학회 2007년도 추계국제학술대회 및 컨퍼런스
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• pp.631-631
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• 2007

• 한국어병학회지
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• 제14권1호
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• pp.3-10
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• 2001

식물성 생약재의 항균력과 활성산소(superoxide anion, $O_2^-$)의 생산에 미치는 in vitro 효과를 조사하여 금후 in vivo 실험에 적용할 때, 어류 체내 자연 저항성 증진을 꾀할 수 있는 식물성 생약재를 발굴하기 위한 기초자료를 얻고자 하였다. 식물성 생약재는 고삼, 귀전우, 구절초, 박하, 방풍, 연교, 약쑥, 지유, 삼백초, 삼지구엽초, 상백피, 소회향, 편축, 팔각 등 14종류로 이들을 열수추출하여 실험에 사용하였다. 항균력 시험에는 어병세균 Listonella anguillarum(Vibrio anguillarum), Vibrio sp., Vibrio alginolyticus, Edwardsiella tarda, Streptococcus sp., Lactcoccus garvieae를 대상으로 disk법을 사용하였으며, 활성산소의 생산능은 넙치 신장 대식세포를 이용한 nitroblue tetrazolium(NBT) 반응으로써 조사하였다. 그 결과,약쑥이 다른 생약재에 비하여 시험에 사용된 어병 세균에 대한 항균활성이 월등히 뛰어났으며, 또한 넙치 대식세포의 활성산소 생산능을 크게 자극하는 효과가 있음을 확인할 수 있었다.

• Molecules and Cells
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• 제38권7호
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• pp.657-662
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• 2015

Rapid and efficient engulfment of apoptotic cells is an essential property of phagocytes for removal of the large number of apoptotic cells generated in multicellular organisms. To achieve this, phagocytes need to be able to continuously uptake apoptotic cells. It was recently reported that uncoupling protein 2 (Ucp2) promotes engulfment of apoptotic cells by increasing the phagocytic capacity, thereby allowing cells to continuously ingest apoptotic cells. However, the functions of Ucp2, beyond its possible role in dissipating the mitochondrial membrane potential, that contribute to elevation of the phagocytic capacity have not been determined. Here, we report that the anion transfer or nucleotide binding activity of Ucp2, as well as its dissipation of the mitochondrial membrane potential, is necessary for Ucp2-mediated engulfment of apoptotic cells. To study these properties, we generated Ucp2 mutations that affected three different functions of Ucp2, namely, dissipation of the mitochondrial membrane potential, transfer of anions, and binding of purine nucleotides. Mutations of Ucp2 that affected the proton leak did not enhance the engulfment of apoptotic cells. Although anion transfer and nucleotide binding mutations did not affect the mitochondrial membrane potential, they exerted a dominant-negative effect on Ucp2-mediated engulfment. Furthermore, none of our Ucp2 mutations increased the phagocytic capacity. We conclude that dissipation of the proton gradient by Ucp2 is not the only determinant of the phagocytic capacity and that anion transfer or nucleotide binding by Ucp2 is also essential for Ucp2-mediated engulfment of apoptotic cells.

• 한국어병학회지
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• 제21권3호
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• pp.219-228
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• 2008

The effects of dietary yeast β-glucan administration on growth, nonspecific immune responses, serum lysozyme, skin mucous lysozyme, NBT (nitroblue tetrazolium) reduction by phagocytes, and disease resistance against Edwardsiella tarda in Japanese eel, Anguilla japonica were evaluated. Fish were fed the diets supplemented with 0%, 0.1% and 0.5% of yeast β-glucan to a commercial diet for 6 weeks. The body weight gain from the fish fed on the 0.5% supplemented diet for 6 weeks was significantly higher than the control. Both serum and skin mucous lysozyme were significantly higher in the all experimental groups except 2 weeks of 0.5% group. The bactericidal activity of serum was slightly increased at 6 weeks. Also, The intracellular superoxide anion production of kidney phagocytes was significantly higher in the all experimental groups. The diet supplemented with 0.1% were also found to raise the relative percent survival (RPS) of Japanese eel after an artificial challenge with 1×107 cells of Edwardsiella tarda per fish. The results suggested the potential of yeast β-glucan to activate some innate immune responses and to improve the growth in Japanese eel.