Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Different invasion efficiencies of Brucella abortus wild-type and mutants in RAW 264.7 and THP-1 phagocytic cells and HeLa non-phagocytic cells

  • Shim, Soojin (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Im, Young Bin (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Myunghwan (Department of Microbiology, Research Institute of Life Sciences, Gyeongsang National University School of Medicine) ;
  • Park, Woo Bin (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University) ;
  • Yoo, Han Sang (Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University)
  • Received : 2018.04.03
  • Accepted : 2018.04.30
  • Published : 2018.06.30
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Abstract

Brucellosis is one of the common zoonoses caused by Brucella abortus (B. abortus). However, little has been reported on factors affecting invasion of B. abortus into host cells. To investigate cell-type dependent invasion of B. abortus, phagocytic RAW 264.7 and THP-1 cells and non-phagocytic HeLa cells were infected with wild-type and mutant B. abortus, and their invasion efficiencies were compared. The invasion efficiencies of the strains were cell-type dependent. Wild-type B. abortus invasion efficiency was greater in phagocytic cells than in epithelial cells. The results also indicated that there are different factors involved in the invasion of B. abortus into phagocytic cells.

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References

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