• Title/Summary/Keyword: Phagocyte

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The Effect of Panax Ginseng Extract on the Immunotoxicity of Cimetidine in Mice (마우스에 있어서 Cimetidine의 면역독성에 미치는 인삼유출물의 영향)

  • 안영근;이상근
    • Environmental Analysis Health and Toxicology
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    • v.6 no.1_2
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    • pp.25-38
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    • 1991
  • The immunopotenciating effects of petroleum ether extract, ethanol extract and butanol fraction of panax ginseng on the immunotoxicity of Cimetidine were investigated in ICR mice. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), and rosette forming cell (RFC) in mice, sensitized and challenged with sheep red blood cells. To investigate the change of the non-specific immune responses, phagocyte activity and number of leukocytes in peripheral blood were measured also. The results of this study are summarized as followings; 1. Cimetidine treated group as compared with normal group generally decreased HA, 2-MER, RFC, number of circulating leukocytes and phagocyte activity whereas in-creased Arthus reaction and DTH. 2. The panax ginseng petroleum ether extract combined administration group as compared with the control group remarkably increased HA, 2-MER, number of circulating leukocytes and phagocyte activity. 3. The panax ginseng ethanol extract combined administration group as compared with the control group remarkably increased Arthus reaction, DTH, HA, RFC, number of circulating leukocytes and phagocyte activity. 4. The panax ginseng butanol fraction combined administration group as compared with the control group remarkably increased Arthus reaction, HA, 2-MER, RFC, number of circulating leukocytes and phagocyte activity.

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Production of Phagocyte Activating Supernatants by Olive Flounder (Paralichthys olivaceus) Leucocytes Stimulated with Genomic DNA of Escherichia coli

  • Lee Chan Hwei;Kim Dong Soo;Kim Ki Hong
    • Fisheries and Aquatic Sciences
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    • v.5 no.4
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    • pp.258-262
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    • 2002
  • Effects of Escherichia coli genomic DNA on the production of phagocyte activating supernatants by the head kidney leucocytes isolated from olive flounder (Paralichthys olivaceus) were investigated. Phagocyte activating activity of the supernatants was estimated by. measuring reactive oxygen species (ROS) production in target head kidney phagocytes. All supernatants from olive flounder head kidney leucocytes-stimulated with E. coli DNA induced significantly (P<0.01) higher ROS production from target pagocytes than the unstimulated control supernatant. Maximum enhancement of chemiluminescent response was observed $5.0-10.0{\mu}g\;mL^{-1}$ of bacterial DNA while the increment ability was decreased significantly (P<0.01) at the concentration of $20.0{\mu}mL^{-1}$. The results demonstrate that olive flounder head-kidney leucocytes stimulated with bacterial DNA release a soluble phagocyte activating cytokines capable of enhancing the respiratory burst activity from target phagocytes.

Effect of Ginseng petroleum Ether Fraction on the Immunotoxicity of Cadmium in Mice (II) (마우스에 있어서 카드뮴의 면역독성에 미치는 인삼 석유 Ether분획의 영향(II))

  • 안영근;김정훈;이상근;황갑수
    • Environmental Analysis Health and Toxicology
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    • v.3 no.3_4
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    • pp.1-8
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    • 1988
  • Experiment was performed to investigate the immunotoxicity of cadmium administered orally and the effect of ginseng petroleum ether fraction on it. Mice were given 3, 30, or 300 ppm cadmium as cadmium chloride orally in the drinking water and injection of ginseng petroleum ether fraction intraperitoneally for 4 weeks. Mice were sensitized and challenged u'ith sheep red blood cells (5-HBC). Immune response was evaluated by delayed type hypersensitivity (DTH), Rosette forming cell (RFC), phagocyte activity, and natural killer cell activity (NK cell activity). In the present study, cadmium suppressed the cellular immunity, It also depressed phagocyte activity very significantly in all cadmium-administered groups, NK cell activity in the cadmium-300 ppm administered group. Ginseng petroleum ether fraction showed restoring effect on the decrease in RFC by cadmium-administration. Remarkably, it showed very significant restoring effect on the depression of phagocyte activity induced by cadmium-administration. From this result, we suppose that the anti-tumor effect of ginseng ether or petroleum ether extract, which has been reported by some other researchers, is mainly due to the increase of phagocyte activity by it's administration.

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Effect of Olive Oil Diet on the Immune Response in ICR Mice (마우스에 있어서 Olive Oil의 식이가 면역반응에 미치는 영향)

  • 안영근;박병철;김정훈;이상근;박영길
    • Environmental Analysis Health and Toxicology
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    • v.6 no.1_2
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    • pp.59-70
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    • 1991
  • This study was performed to investigate the effect of olive oil diet on the immune response in ICR male mice. Experimental diets of 4 groups were fed ad libitum to the ICR male mice for 27 days. The results of this study were summarized as followings: 1. 10% Olive oil diet group as compared with the control diet group significantly decreased liver weight rate but significantly increased hemagglutination titer (HA), Arthus reaction, delayed type reaction (DTH), rosette forming cell (RFC), and phagocyte activity. 2. 20% Olive oil hypersensitivity diet group as compared with the control diet group significantly increased body weight gain, liver weight rate, and HA but significantly decreased Arthus reaction, DTH, RFC, phagocyte activity, and peripheral circulating white blood cell (WBC). 3. 30% Olive oil diet group as compared with the control diet group significantly increased liver weight rate but significantly decreased body weight gain, Arthus reaction, plaque forming cell (PFC), DTH, RFC, phagocyte activity, and WBC. The results showed that the increase of olive oil doses significantly decreased humoral and cellular immune responses, phagocyte activity, and WBC.

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The Effect of Cimetidine, Ranitidine and Famotidine on the Immune Response in ICR Mice (마우스에 있어서 Cimetidine, Ranitidine 및 Famotidine이 면역반응에 미치는 영향)

  • 안영근;김정훈;이상근
    • Environmental Analysis Health and Toxicology
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    • v.5 no.3_4
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    • pp.37-45
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    • 1990
  • Experiments were performed on mice to investigate the influences of cimetidine, ranitidine and famotidine on the immune response. Immune response were evaluated by antibody, Arthus reaction (Arthus), delayed type hypersensitivity (DTH), rosette forming cell (RFC), phagocyte activity and whit( blood cell (WBC) in mice, sensitized and challenged with sheep red blood cells (SRBC). The weight of liver, spleen and thymus were measured. Following results obtained in this experiment. 1) The administration of cimetidine as compared to normal group significantly decreased Arthus, Hemagglutinin titer (HA), RFC, DTH, WBC and phagocyte activity, but increased the activity of serum albumin. 2) The administration of ranitidine as compared to normal group decreased RFC and HA. 3) The administration of Famotidine as compared to normal group decreased DTH and RFC, and significantly decreased HA, Arthus and serum protein. 4) The administration of ranitidine and famotidine decreased more humoral immune response than cellular immune response, but the administration of cimetidine significantly decreased humoral and cellular immune response, WBC and phagocyte activity.

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Regulation of the Phagocyte Respiratory Burst Oxidase by Protein Interactions

  • Lambeth, J. David
    • BMB Reports
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    • v.33 no.6
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    • pp.427-439
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    • 2000
  • The activity of the phagocyte respiratory burst oxidase is regulated by complex and dynamic alterations in protein-protein interactions that result in the rapid assembly of an active multicomponent NADPH oxidase enzyme on the plasma membrane. While the enzymatic activity has been studied for the past 20 years, the past decade has seen remarkable progress in our understanding of the enzyme and its activation at the molecular level. This article describes the current state of knowledge, and proposes a model for the mechanism by which protein-protein interactions regulate enzyme activity in this system.

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Effect of Ethanol on the Immunotoxicity of Ampicillin in Mice (Ampicillin의 면역독성에 미치는 Ethanol의 영향)

  • 안영근;김정훈;나헌진
    • Environmental Analysis Health and Toxicology
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    • v.3 no.1_2
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    • pp.55-64
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    • 1988
  • Experiments were performed on mice to investigate the influences of ampicillin and ethanol on the immune response. Ampicillin was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. 1. The combined administration of ampicillin and ethanol as compared to ampicillin had not influence on the weight of spleen, but increased the weight of thymus. 2. Humoral immune response was slightly reduced by ampicillin. Especially, the combined administration of ampicillin and ethanol significantly reduced hemclysin production. 3. Cellural immune response was reduced by ampicillin. The combine administration of ampicillin and ethanol significantly reduced cellural immune response. 4. Peripheral circulating white blood cell was reduced by the combined administration of ampicillin and ethanol as compared to ampicillin. 5. The combined administration of ampicillin and ethanol as compared to ampicillin had not influence on the phagocyte activity.

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Effect of Ethanol on the Immunotoxicity of Doxycycline in Mice (마우스에 있어서 Doxycycline의 면역독성에 미치는 Ethanol의 영향)

  • 안영근;김정훈;정지건
    • Environmental Analysis Health and Toxicology
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    • v.4 no.1_2
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    • pp.1-10
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    • 1989
  • Experiments were performed on mice to investigate the influences of doxycycine and ethanol on the immune responses. Doxycycline was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. Pathotoxicological influences were measured by serum protein and albumin. The weight of spleen, thymus and liver were measured. Doxycline and ethanol combined administration decreased the weight of thymus and spleen. Humoral and cellular immune response were reduced by doxycycline administration. Especially ethanol combined administration significantly reduced humoral and cellular immune response. Phagocyte activity was increased by ethanol combined administration and peripheral circulating white blood cell was significantly increased by ethanol adminstration. Ethanol combined administration decreased serum A/G ratio.

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Effects of the Butanol Fraction of Astragali Radix on the Cellular Immune Function in Mice (황기 부탄올 분획물이 생쥐의 세포성 면역기능에 미치는 영향)

  • 김정훈;문연자;이성원;이승연;우원홍;박정숙
    • YAKHAK HOEJI
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    • v.46 no.1
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    • pp.52-57
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    • 2002
  • Effects of the butanl fraction of Astragali Radix (BFAR) on the cellular and nonspecific immune responses were investigated in ICR mice. Mice were divided into 4 groups and BFAR at doses of 5, 25 and 125 mg/kg u ere administered orally to mice daily for 3 weeks, and the normal animals were given vehicle. The results of this study are summarized as follows; the relative weight of thymus was greatly increased by BFAR treatment, compared with that in mormal mice. However, the body weight gain was not affected. Delayed-type hypersensitivity (DTH) reaction to sheep red blood cells (SRBC) for cellular immunity was significantly enhanced by BFAR treatment, compared with those in normal mice. In these mice, BFAR also dose-dependently increased activities of phagocyte and natural killer (NK) cells as well as the number of leukocyte resulted from nonspecific immunity Thus, these results demonstrate that BFAF treatment results in a significant increase in both cellular and nonspecific immune responses to antigen in concentrations that enhance humoral immune function.