• 한국약용작물학회지
• /
• 제13권1호
• /
• pp.21-29
• /
• 2005

경남지역의 자생 둥굴레속 47개 수집종의 유연관계를 분석하기 위한 기초자료로서 수집종의 생장 몇 형태 관련 형질, 잎마름병 감염정도, 이들 형질간 상관관계를 분석하였던 바 그 결과는 다음과 같다. 1. 초장은 산둥굴레에서 가장 길었던 반면, 용둥굴레에서 가장 짧았다. 한편 줄기의 생장형태는 직립형을 포함한 3개군으로, 줄기색깔은 녹색을 포함한 3개군으로 분류하였다. 2. 염의 형태로는 타원형을 포함한 5개군으로, 엽병은 존재 유무로부터 2개군으로, 엽병의 색깔은 녹색을 포함한 3개군으로 분류되었으며, 그 중에 특이하게 피침 형태로 엽연이 자색인 1개종이 수집되었다. 3. 꽃은 화형이 항아리형, 통형, 조롱박형의 3개군으로, 화색은 흰색을 포함한 3개군으로 분류되었으며, 특별하게 조롱박형인 1개 수집종은 연녹색을 띄고 있었다. 화사는 실린더형과 편평형으로, 화경의 색깔은 자색을 포함한 4개군으로 분류되었으며, 포의 존재 유무와 형태에 따라 4개군으로 분류하였다. 4. 과실은 둥근형 등 3개군으로 분류되었으며, 백과중은 둥굴레에서 가장 무거웠던 반면, 용둥굴레에서 가장 가벼웠다. 5. 둥굴레 잎마름병에 대한 저항성이 아주 강한 것은 2종, 이병율이 7% 이하인 것은 7개종으로 조사되어서 근경수량을 증대할 수 있는 저항성 유전자원이 자생하고 있음을 알 수 있었다. 6. 제형질의 상관관계에서 근경수량과 관련된 근경중은 초장, 경직경, 엽수, 엽장, 엽폭, 지하경 직경과 정의상관을 보였으나 근경수량에 가장 큰 영향을 미치는 잎마름병 이병율은 초장, 경직경, 엽수, 엽장, 백립중, 근경중, 지하경 직경과 부의상관을 보였다.

• 현장농수산연구지
• /
• 제5권1호
• /
• pp.28-35
• /
• 2003

화성지역 '캠벨얼리' 포도 포도원의 엽병의 무기성분, 수체생육 및 과실품질을 조사하였다. 토양의 물리·화학성은 Y포도원은 적정기준 농도보다 pH는 높고 유기물 함량은 낮으며 인산은 2.8배, 칼륨은 3.6배 높았으나 칼슘, 마그네슘, 양이 온치환용량은 큰 차이가 없었다. H포도원은 pH와 유기물함량은 낮고 인산, 칼륨, 칼슘 및 마그네슘 함량은 적당하였다. C포도원은 유기물함량은 낮은 편이며 인산은 3.5배 높았으나, pH, 칼륨, 칼슘, 마그네슘 및 양이온치환용량은 적당한 편이었다. 그러나 심토는 배수 잘 안 되는 토양이었다. 엽병의 질소, 칼슘 및 마그네슘 함량은 적당하였으나 인산은 Y 및 C포도원에서 기준치보다 8~6배, 칼리는 Y포도원에서 1.8배 높았다. 포도원간 과실 경도, 착색정도 및 과분발생정도는 큰 차이는 없었으나, 가용성고형물, 주당수량 및 상품과율은 Y와 H포도원에 비하여 C포도원가 현저히 떨어졌다. 생리장해 및 병 발생은 Y포도원에서 동녹 발생이 H포도원은 착색불량과가 C포도원은 수정불량과, 열과 및 탄저병 발생이 많았다.

• 원예과학기술지
• /
• 제17권1호
• /
• pp.23-28
• /
• 1999

Prunus cerasifera, P. domestica 및 P. salicina에서 기원된 자두 53 종 또는 품종으로 부터 27 형태형질의 특성을 조사하여 품종 동정에 필요한 기초자료를 제공하고, 그들간의 분류적 관계를 알아보기 위하여 주성분분석 및 집괴분석을 실시하였다. 조사된 형태형질 중 잎의 크기, 잎의 형태 및 엽내 털 발생정도 등이 자 두아속 식물의 동정 및 분류적 관계 해석에 있어서 상당히 유용하게 이용될 수 있을 것으로 단되었으며, 잎의 길이, 엽병 길이, 밀선수, 형, 엽저, 만개기 등은 P. domestica계 자두와 P. salicina계 자두간에 명확한 차이를 나타내었다. 또한 주성분분석 득점치를 이용한 비가 평균결합법에 의한 집괴분석 결과 53개 분류 군들은 평균거리 1.0을 기준으로 할 때 P. alicina-P. cerasifera 표현군, P. domestica 표현군 및 P. pinosa 표현군 등 3개의 표현군으로 분류될 수 있어, 분류군들의 대략적인 분류적 관계 분석이 가능하였다. 그러나 정확한 종군 분류를 위해서는 보다 많은 형태형질의 용이 필요할 것으로 판단되었다.

• Journal of Plant Biotechnology
• /
• 제4권4호
• /
• pp.171-178
• /
• 2002

Chlorophyll (Chl) fluorescence imaging was used to investigate the effectiveness of in vivo incorporation methods for two chemicals, 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) and methyl viologen (MV) in rice, a monocot, and cucumber, a dicot, leaves. four different methods (vacuum infiltration, floating, transpiration-aided incorporation through petiole and spraying) were compared, and $F_i$ and $F_v$/$F_m$ were chosen for the imaging of the DCMU- and MV-treated leaves, respectively. The effects of the chemicals in plants were generally heterogeneous over the whole leaf area. Moreover, the effectiveness of the treatment of a chemical in plant leaves was dependent on chemical species, plant species, concentration of the chemical, the treatment method, the duration of the treatment, the existence of light and detergent, etc. In conclusion, we suggest that to achieve the proposed effects of chemicals in plants for an actual experiment, these factors must be considered before the chemical treatment, and the best method for the treatment of the chemicals tested was floating and vacuum infiltration in the dicot and the monocot leaves, respectively, as drawn from Chl fluorescence imaging analysis.

• Tribology and Lubricants
• /
• 제33권6호
• /
• pp.251-255
• /
• 2017

In this study, we aimed to clarify the wettability and anisotropic flow characteristics of rice leaves as a basic study for engineering applications of anisotropic flow characteristics of rice leaf surface. To investigate the surface structure of rice leaf, the micro grooves and asperities of rice leaves were analyzed and quantified by scanning electron microscope, Confocal laser scanning microscopy, and stylus profilometer. The analysis of the structure of rice leaf surface confirmed that asymmetrical cone - like protrusions in leaf veins were inclined toward the leaf tip. The static contact angle test showed that the contact angle at the midline vein or leaf vein location where the micropapilla is concentrated is about $20^{\circ}$ higher than the leaf blade position. The contact angles of fresh and dried rice leave were also compared. The dried rice leaves showed a contact angle of about $5^{\circ}$ to $15^{\circ}$ higher than that of fresh leaves, suggesting that the volume of the protrusions decreased as the water was removed, thus reducing the contact area with the droplet. In the contact angle history test the hysteresis in the leaf tip direction was found to be much lower than that in the leaf petiole direction. This results can be explained that asymmetrical cone - like protrusions had a significant effect on the droplet flow characteristics through contact angle hysteresis experiment.

• Plant Biotechnology Reports
• /
• 제2권4호
• /
• pp.253-258
• /
• 2008

The cDNA of the touch-induced genes (TCH) of the sweet potato [Ipomoea batatas (L.) Lam.] has been cloned and analyzed. IbTCH1, which exists as at least two-copy genes in the genome of the sweet potato, encodes for 148-amino acid polypeptides, and harbors four conversed $Ca^{2+}-binding$ motif EF-hands. IbTCH1 was shown to be expressed in the flower, leaf, thick pigmented root, and particularly in the white fibrous root, but expressed only weakly in the petiole. IbTCH1 is upregulated upon exposure to environmental stresses, dehydration, and jasmonic acid. Furthermore, IbTCH1 is developmentally regulated in the leaf and root. These results strongly indicate that the gene performs functions in both plant development and in defense/stress-signaling pathways.

• Journal of Plant Biology
• /
• 제22권1_2호
• /
• pp.21-27
• /
• 1979

Sandy loam plots of pH 5.5, which was created by the reclamation of highlands in Yongin county, Kyeonggi province, was selected for the present experimental study. Sugar beet was cultured in these plots by treating them with calcium hydroxide and 3 replications to maintain pH 5.5, 6.4, 6.8, 7.2, and 7.6, respectively. The growth and yield of sugar beet were investigated and the results obtained showed a great deal of boron deficiency. The growth in its early stage was favorable in high pH plots, while no significant difference was observed in sugar content, root weight and sugar production at the time of harvest in each treated plot. The tendency of sugar accumulation appeared to be identical in terms of pattern in each treated plot; i.e. sugar was accumulated until early September but declined momentarily from the middle of September and then the sugar accumulation was resumed from the middle of October. The number of individuals in boron deficiency was larger in high pH plots and the condition of such boron deficiency was also severe. The result of analysis of boron contained in plant body revealed that the boron content of individuals caused boron deficiency by water culture and that occurred in the plots was small alike. The appearance of boron deficiency is characterized by the emergence of black stripes on the surface of the basal part of petiole, severe necrosis of young leaves in the area of growing point and severe wrinkles. Therefore it is felt necessary to pay particular attention to boron deficiency in the culture of sugar beet in our country.

• Plant Resources
• /
• 제6권3호
• /
• pp.183-187
• /
• 2003

Two kinds of Angelica belong to Umbelliferae collected, the one is Angelica gigas that is inhabitant in Korea and the other is Angelica acutiloba that is indigenous in Japan at the field of Snyder Research and Extension Farm Rutgers University, New Jersey and was analyszed by GC and GC/MS. The composition of the essential oil of the different aerial parts of the Angelica has been studied. The oil yields obtained upon hydrodistillation were 0.18% (v/w) in Korean Angelica and 0.44% (v/w) in Japanese Angelica on dry root weight basis. By the growing stage in the Rutgers greenhouse condition, leaf and root of essential oil content a little decreased on 9 months later than 4 months later except for Angelica gigas leaf. Both of Angelica showed that amounts of essential oil content presented in order of leaf > petiole > root according to different plant part. The analysis of the essential oil from Angelica root led to the identification of 14 constituents totaling 64% in Korean Angelica and 13 constituents totaling 68% in Japanese Angelica. The major constituents of the Angelica root essential oil were ligustilide (47 %) and gamma terpi (14 %) in Korean Angelica, and alpha pinei (32 %) and nonane (25 %) in Japanese Angelica

• 식물조직배양학회지
• /
• 제25권2호
• /
• pp.89-93
• /
• 1998

Tomato phenylalanine ammonia-lyase 5 (tPAL5) was identified that alternate initiation sites were utilized differentially in response to environmental stimuli (Lee et al, 1992b). In this study, we tried to look into tissue -or cell- specific expression pattern of tPAL5 gene by fusing with ${\beta}-glucuronidase$ (GUS) gene in transgenic tobacco plants. In transgenic plants, root and stem extracts contained 8~12 fold higher levels of GUS activity than petiole or leaf tissue while the highest levels of induction was observed from leaf tissue by mechanical wounding (5~11 fold). In trans-sections of stems and petioles, GUS activity was restricted to phloem cells(outer region) of developing vascular bundle and mainly at apical tip region in the root tissues. The levels of GUS activity was drastically reduced (10~12 fold reduction) when the 5'-upstream region of tPAL5 gene (-1151bp from ATG codon) was deleted up to -665. The levels of GUS expression, however, raised up by 6~8 fold when deleted up to -455. Therefore, we conclude that there are positive cis-elements at the region -1151 to -1008 and at -455 to -195 while the negative cis-element is at -1008 to -455.

• 한국육종학회지
• /
• 제42권5호
• /
• pp.431-438
• /
• 2010

Anthers of sweet pepper $F_1$ cultivar 'Special' were cultured on Dumas De Vaulx (C medium), supplemented with $0.1mgL^{-1}$ 2, 4-D and $0.1mg{\cdot}L^{-1}$ kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and $9.8^{\circ}Brix$, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.