Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

In vivo Monitoring of the Incorporation of Chemicals into Cucumber end Rice Leaves by Chlorophyll Fluorescence Imaging

  • Kim, Jin-Hong (Department of Molecular Biology, Pusan National University) ;
  • Jung, Ji-Eun (Department of Molecular Biology, Pusan National University) ;
  • Lee, Choon-Hwan (Department of Molecular Biology, Pusan National University)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Chlorophyll (Chl) fluorescence imaging was used to investigate the effectiveness of in vivo incorporation methods for two chemicals, 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) and methyl viologen (MV) in rice, a monocot, and cucumber, a dicot, leaves. four different methods (vacuum infiltration, floating, transpiration-aided incorporation through petiole and spraying) were compared, and $F_i$ and $F_v$/$F_m$ were chosen for the imaging of the DCMU- and MV-treated leaves, respectively. The effects of the chemicals in plants were generally heterogeneous over the whole leaf area. Moreover, the effectiveness of the treatment of a chemical in plant leaves was dependent on chemical species, plant species, concentration of the chemical, the treatment method, the duration of the treatment, the existence of light and detergent, etc. In conclusion, we suggest that to achieve the proposed effects of chemicals in plants for an actual experiment, these factors must be considered before the chemical treatment, and the best method for the treatment of the chemicals tested was floating and vacuum infiltration in the dicot and the monocot leaves, respectively, as drawn from Chl fluorescence imaging analysis.

Keywords

References

  1. Plant Physiol v.104 Diagnosis of the earliest strain-specific interactions between tobacco mosaic virus and chloroplasts of tobvacco leaves in vivo by means of chlorophyll fluorescence imaging Balachandran S;Osmond CB;Daley PF https://doi.org/10.1104/pp.104.3.1059
  2. Photosynthetica v.30 Imaging of the blue, green, and red fluorescence emission of plants: An overview, Buschmann C;Langsdorf G;Lichtenthaler HK https://doi.org/10.1023/A:1012440903014
  3. Aust J Plant Physiol v.22 Quantitative mapping of leaf photosynthesis using chlorphyll fluorescence imaging Genty B;Meyer S https://doi.org/10.1071/PP9950277
  4. Photosynthetica v.38 Seeing is believing Govindgee;Nedbal L https://doi.org/10.1023/A:1012485018944
  5. Naturwissenschaften v.48 Neue Versuche zur Kohlensauer assimilation Kautsky H;Hirsch A
  6. Plant Cell Physion v.42 Inhibition of photosystems Ⅰ and Ⅱ and enhanced back flow of photosystem Ⅰelectrons in cucumber leaf discs chilled in the light Kim S-J;Lee C-H;Hope AB;Chow WS https://doi.org/10.1093/pcp/pce109
  7. Photosynthetica v.38 Multicolour fluorescence imaging of sugar beet leaves with different nitrongen status by flash lamp UV-excitation Langsdorf G;Buschmann C;Sowinska M;Babani F;Mokry M https://doi.org/10.1023/A:1012409423487
  8. Planta v.212 Photoinactivation of photosystem Ⅱ complexes an photoprotection by nonfunctional neighbors in Capsicum amnuum L. leaves. Lee, H-Y;Hong Y-N;Chow WS https://doi.org/10.1007/s004250000398
  9. Photosynthetica v.38 Measurement of differences in red chlorophyll fluorescence and photosynthetic between sun and shade leaves by fluorescence imaging Lichtenthaler HK;Babani F;Langsdorf G;Buschmann C https://doi.org/10.1023/A:1012453205740
  10. J Plant Physiol v.148 Detection of vegetation stress via a new high resolution fluorescence imaging system Lichtenthaler HK;Lang M;Sowinska M;Heisel F;Miehe JA https://doi.org/10.1016/S0176-1617(96)80081-2
  11. Photosynth Res v.66 Kinetic imaging of chlorophyll fluorescence using modulated light Nedbal L;Soukupov J;Kaftan D;Whitmarsh J;Trtrlek M https://doi.org/10.1023/A:1010729821876
  12. Appl Spectrosc v.49 Imaging fluorometer to detect pathological and physiological change in plants Ning L;Edwards GE;Strobe GA;Daley LS;Callis JB https://doi.org/10.1366/0003702953965542
  13. J Photosci v.8 Different susceptibilities to low temperature photoinhibition in the photoynthetic apparatus among three cultivars of cucumber (Cucumis sativus L.) Oh K-H;Lee WS;Lee C-H
  14. Plant Physiol v.84 Image analysis of chlorophyll fluorescence transients for diagnosing the photosynthetic system of attached leaves Omasa K;Shimazaki KI;Aiga I;Larcher W;Onoe M https://doi.org/10.1104/pp.84.3.748
  15. Bot Acta v.111 Chlorophyll fluorescence quenching during photosynthetic induction in leaves of Abutilon mosaic virus, observed with a field-portable imaging system Osmond CB;Daley PF;Badger MR;Lttge U https://doi.org/10.1111/j.1438-8677.1998.tb00724.x
  16. Photosynth Res v.54 Resolving chlorophyll a fluorescence images of photosyntheti efficiency into photochemical and non-photochemical components calculation of qP and Fv/Fm without measuring Fo Oxborough K;Baker NR https://doi.org/10.1023/A:1005936823310
  17. Planta v.199 Photosynthesis in localized regions of oat leaves infected with crown rust (Puccinia coronata): Quantiative imaging of chlorophyll fluorescence Scholes JD;Rolfe SA
  18. Photosynth Res v.45 Imaging of chlorophyll-a fluorescence in leaves: Topography of photosynthetic oscillations in leaves of Glechoma hederacea Siebke K;Weis E https://doi.org/10.1007/BF00015563