• 농촌계획
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• 제10권4호
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• pp.55-64
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• 2004

National Agricultural Pesticide Risk Analysis (NAPRA) WWW 시스템 (http://pasture.ecn.purdue.edu/${\sim}napra$)은 각기 다른 영농방법이 지표수질, 유사, 그리고 지하수질에 미치는 영향을 평가하기 위하여 개발되었다. 이 NAPRA WWW 시스템은 Total Maximum Daily Loads와 같은 수질 요건을 만족시킬 수 있는 최적영농 방법이 무엇인지 찾는데, 그리고 수질측면에서 취약한 지역을 찾아내는데 매우 효율적인 시스템이다. 이 NAPRA WWW 시스템을 이용하여 미국 인디애나주의 수계에 대해서, NAPRA 모의 Nitrogen과 Atrazine 결과를 실측치와 비교하였다. 18개 수계에 대해서 NAPRA 예측 질소값과 실측 질소값을 비교한 결과 $R^2$ 값은 0.51이고, 6개 수계에 대해서 NAPRA 예측 Atrazine값과 실측값을 비교한 결과 $R^2$ 값은 0.87이었다. 이 연구에서 보여지는 바와 같이 NAPRA WWW 시스템은 수계내에서 질소와 Atrazine에 따른 오염지역을 찾아내는데 효율적으로 사용될 수 있는 시스템이다.

• 대한임상독성학회지
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• 제13권2호
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• pp.55-61
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• 2015

Purpose: Many studies have examined the mechanisms of impaired glucose homeostasis after organophosphate (OP) exposure, however no study has evaluated the clinical utility of blood glucose measurements in patients with OP poisoning. The current study was conducted to evaluate the initial glucose level at presentation and the glycemic variables during the first 3 days after admission as a predictor of mortality. Methods: This retrospective observational case series included 228 patients with a history of OP poisoning. Among other clinical data, information on the initial glucose level at presentation and mean glucose level, delta glucose level, and the presence of a hypoglycemic event during the first 3 days of admission, was collected. Results: Survivors had lower initial glucose levels at presentation and glucose variability during the first 3 days of admission compared to non-survivors. The frequency of hypoglycemic events was higher in non-survivors. In multivariate analysis, the initial glucose level (> 233 mg/dl) was an independent predictor of mortality, along with age. Conclusion: The initial glucose level at presentation can be helpful in prediction of mortality in cases of OP intoxication at bedside. The physician should pay attention to patients with a glucose level >233 mg/dl at presentation after ingestion of OP.

• 한국환경과학회지
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• 제18권11호
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• pp.1247-1259
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• 2009

We investigated the toxic effects of carbaryl on early embryo development in the African clawed frog, Xenopus laevis. To test the toxic effects, frog embryo teratogenesis assays using Xenopus were performed. Embryos were exposed to various concentrations of carbaryl ($5{\sim}320\;{\mu}M$). $LC_{100}$ for carbaryl was $320\;{\mu}M$, and the $LC_{50}$ determined by probit analysis was the concentration of $235.68\;{\mu}M$. Exposure to $160\;{\mu}M$ of carbaryl resulted in 10 different types of severe external malformations. Histological examination revealed dysplasia of the eyes, heart, guts, somatic muscle, dorsal, liver, blood vessel and swelling of the pronephric ducts. Malformation of neural tissue and brain was not severe even in the high dose of carbaryl. Benzidine blood stain showed distinct inhibition of inducing erythrocytes in embryos and animal cap explants. Electron micrographs of embryo revealed retinal detachment, loose photoreceptor lamella and the degeneration of sarcomeres in the carbaryl-treated group. The mitochondrial degeneration was also observed in the test group.

• Environmental Analysis Health and Toxicology
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• 제13권1_2호
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• pp.33-41
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• 1998

Chlorpyrifos, o,o diethyl-o-(3,5,6-trichloro-2-pyridyl) phosphorothioate, is a broad spectrum organophosphate insecticide. The use of chlorpyrifos has been increased more and more as pesticide. But the effects of chlorpyrifos on the immune alterations has not been yet observed. Therefore, we investigated the effects of chlorpyrifos on the immune alterations in CICA male mice. Chlorpyrifos was administered to mice by a single intraperitoneal injection for the purpose of observing acute effects. On the one hand to get the information on immunopathologic alterations we observed hematological values, counted total circulating leukocytes and assessed the ratio of lymphocytes and neutrophils from the peripheral blood, measured the ratio of organ/body weight and counted splenic cellularity in CBA male mice which treated chlorpyrifos intraperitoneally. But we could not find any significant immunopathologic alterations statistically by a single intraperitoneal injection. Also, the exposure of chlorpyrifos caused no significant change in the number of PFC/10$^6$ spleen cells at any three given doses. On the other hand a singte intraperitoneal injection of chlorpyrifos decreased the lymphocyte proliferation response slightly to ConA or LPS stimulation at a dose of 6 mg/kg b.w. Administrations of chlorpyrifos reduced mixed leukocyte response(MLR). MLR was decreased moderately at doses of 3mg/kg b.w. and 6mg/kg b.w. Therefore, all these findings suggest that chlorpyrifos may alter the immune functions acutely. especially by the changes of T lymphocyte activity.

• 동아시아식생활학회지
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• 제27권4호
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• pp.450-458
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• 2017

The purpose of this study was to investigate the levels of risk perception characteristics for food risk elements using a psychometric paradigm from 298 university students in Yeungnam region, Korea, by a self-administered questionnaire. The respondents showed the highest level of risk concern about radioactive contaminated foods and the lowest level about GM (genetically modified) foods. In the risk perception characteristics for food risk elements, they perceived radioactive contaminated foods as a catastrophic, worried, new, and uncontrollable risk. In addition, they regarded food additives and foodborne illness as a chronic, controllable, old, and scientifically and individually known risk. According to the results of the factor analysis for risk perception characteristics, dread and unknown were categorized. In the risk perception map, mad cow disease, heavy metal contaminated foods, and radioactive contaminated foods were considered as a dreaded and unknown risk, whereas pesticide residues and GM foods were perceived as a less dreaded and unknown risk. Additionally, food additives and foodborne illness were regarded as a less dreaded and known risk and endocrine disruptors and avian influenza as a dreaded and known risk. These results imply that risk perception characteristics of consumers should be considered to establish strategies for risk communication in food science.

• 농업과학연구
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• 제28권1호
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• pp.48-58
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• 2001

This study was conducted to investigate the effects of environmental sound rice farming method on the productivity of rice industry which may result in impacts on the staple food securities. Recently fanners have been concerned about adopting new rice cultivation method such as organic and low input farming system in which fertilizer and pesticide can be substantially reduced so as to alleviate the burden of agro-ecosystem. However, It has been argued about whether or not there are negative impacts on the self sufficiency rate of food, income of farm household and technological adaptability. Therefore this study examined the productivity trend of environmental rice farming system and predicted the long term rice self-sufficiency rate when environmental rice farming system are adopted by assuming various scenarios. It was estimated that rice self-sufficiency rate can be decreased up to 52.2% by 2010. Based on the analysis of results, policy recommendations for environmentally sound rice farming were suggested as follows: 1) gradual adopting and transferring of environmental rice cultivation method, 2) increasing profitability of rice growing fanners 3) developing the farm level technology specific to Korean farming condition, 4) institutionalization of direct payments for encouraging environmental rice fanning.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.946-951
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• 2008

The opd gene, encoding organophosphorus hydrolase (OPH) from Flavobacterium sp. capable of degrading a wide range of organophosphate pesticides, was surface- and intracellular-expressed in Synechococcus PCC7942, a prime example of photoautotrophic cyanobacteria. OPH was displayed on the cyanobacterial cell surface using the truncated ice nucleation protein as an anchoring motif. A minor fraction of OPH was displayed onto the outermost surface of cyanobacterial cells, as verified by immunostaining visualized under confocal laser scanning microscopy and OPH activity analysis; however, a substantial fraction of OPH was buried in the cell wall, as demonstrated by proteinase K and lysozyme treatments. The cyanobacterial outer membrane acts as a substrate (paraoxon) diffusion barrier affecting whole-cell biodegradation efficiency. After freeze-thaw treatment, permeabilized whole cells with intracellular-expressed OPH exhibited 14-fold higher bioconversion efficiency ($V_{max}/K_m$) than that of cells with surface-expressed OPH. As cyanobacteria have simple growth requirements and are inexpensive to maintain, expression of OPH in cyanobacteria may lead to the development of a low-cost and low-maintenance biocatalyst that is useful for detoxification of organophosphate pesticides.

• 드라이브 ㆍ 컨트롤
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• 제20권3호
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• pp.25-34
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• 2023

In this study, we conducted a research on optimizing the spraying performance of agricultural drones and predicted the spraying performance in various flight conditions using the multi-layer perceptron (MLP). Data was collected using a test device for pesticide spraying performance according to the water sensitive paper (WSP) evaluation. MLP training involved supervised learning to achieve a coefficient of variation (CV), which indicates the degree of uniform spraying. The performance evaluation was conducted using R-squared (R²), the test samples showed an R² of 0.80. The results of this study showed that drone spraying performance can be predicted under various flight environments. In addition, the correlation analysis between flight conditions and predicted spraying performance will be useful for further research on optimizing the spraying performance of agricultural drones.

• 한국유기농업학회지
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• 제31권4호
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• pp.441-451
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• 2023

Golden apple snails (Pomacea canaliculata) were first introduced as food for high protein source and were later widely used as a means of weeding in rice paddies by taking of their vigorous feeding habits. Since 2000, the wintering of golden snails has been found and damage to rice plants occurred by them. To prevent damage, a means to control golden apple snails has become necessary. In this study, we investigated the possibility of eco-friendly control of golden apple snail by using Styrax japonicus fruit that grow naturally in-country. When the ethanol extract of the fruit was treated for controlling golden apple snail, the effect of over 90% was confirmed at 250 and 125 ppm after 1 day. Using UPLC MS analysis, two major compounds, dihydrorotenone known as a natural pesticide was detected. The molluscicidal activity was confrimed at 50 ppm of dihydrorotenone standard material. Our results showed that the possibility of developing an eco-friendly agent of golden apple snail using S. japonicus fruit was confirmed.

• 대한화학회지
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• 제29권5호
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• pp.503-509
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• 1985

농산물에 잔류되어 있는 16종의 유기염소제 농약들을 추출하여 cleanup한 것을 기체 액체 크로마토그래피법으로 분리, 정량하였다. 5% QF-1 정지상의 분리관을 주로 사용하였으며 3% OV-17관과 DC-200/2.5% QF-1 혼합관을 보조적으로 사용하였다. $^{63}Ni$ electron capture detector를 사용하여 각 농약 성분을 검출하였다. 대부분의 농약에 대한 감응곡선은 직선성을 나타냈고 일부는 약간 휘었으나 정량에는 별 지장을 주지 않았다. 16종의 농약을 13종의 농산물에 가하여 분석한 결과 대부분은 85% 이상의 평균회수율을 보였으며 각 5회 분석결과에 대한 상대평균편차는 12.1% 이하로서 AOAC에서 정한 기준을 만족하였다.