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http://dx.doi.org/10.5322/JES.2009.18.11.1247

The Toxic Effects of a Pesticide Carbaryl on the Development of African Clawed Frog, Xenopus laevis  

Shin, Sang-Hee (Department of Biology, Changwon National University)
Lee, Mi-Ju (Department of Biology, Changwon National University)
Lee, Yu-Hwa (Department of Biology, Changwon National University)
Cheong, Seon-Woo (Department of Biology, Changwon National University)
Yoon, Chun-Sik (Department of Biology, Changwon National University)
Publication Information
Journal of Environmental Science International / v.18, no.11, 2009 , pp. 1247-1259 More about this Journal
Abstract
We investigated the toxic effects of carbaryl on early embryo development in the African clawed frog, Xenopus laevis. To test the toxic effects, frog embryo teratogenesis assays using Xenopus were performed. Embryos were exposed to various concentrations of carbaryl ($5{\sim}320\;{\mu}M$). $LC_{100}$ for carbaryl was $320\;{\mu}M$, and the $LC_{50}$ determined by probit analysis was the concentration of $235.68\;{\mu}M$. Exposure to $160\;{\mu}M$ of carbaryl resulted in 10 different types of severe external malformations. Histological examination revealed dysplasia of the eyes, heart, guts, somatic muscle, dorsal, liver, blood vessel and swelling of the pronephric ducts. Malformation of neural tissue and brain was not severe even in the high dose of carbaryl. Benzidine blood stain showed distinct inhibition of inducing erythrocytes in embryos and animal cap explants. Electron micrographs of embryo revealed retinal detachment, loose photoreceptor lamella and the degeneration of sarcomeres in the carbaryl-treated group. The mitochondrial degeneration was also observed in the test group.
Keywords
Carbaryl; Toxic effects; Xenopus laevis; Malformation; Frog;
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1 Relyea R. A. and N. MiIIs, 2001, Predator-induced stress makes the pesticide carbaryl more deadly to gray treefrog tadpoles (Hyla versicolor), Proc. Natl. Acad. Sci. USA., 98(5), 2491-2496   DOI   ScienceOn
2 Stuart S. N., J. S. Chanson, N. A. Cox, B. E. Yong, A. S. Rodrigues, D. L. Fischman and R. W. WaIIer, 2004, Status and trends for amphibian declines and extinctions worldwide, Science 306, 1783-1786   DOI   ScienceOn
3 Yoon C. S., J. H. Jin, J. H. Park, C. Y. Yoe, S. J. Kim, Y. G. Hwang, S. J. Hong and S W. Cheong, 2008, Toxic effects of carbendazim and n-butyl isocyanate, metabolites of the fungicide benomyl, on early development in the African clawed frog, Xenopus laevis, Environ. ToxicoI., 23(1), 131-144   DOI   ScienceOn
4 Hemmati-Brivanlou A. and G. H. R. Thomsen, 1995, Ventral mesodermal patterning in Xenopus embryos:expression patterns and activities of BMP-2 and BMP-4, Dev. Genet., 17(1), 78-89   DOI   ScienceOn
5 Ariizumi T. and M. Asashima, 1994, In vitro control of the embryonic form of Xenopus laevis by activin A: Time and dose-dependent inducing properties of activin-treated ectoderm, Dev. Growth Differ. 36, 499- 507   DOI   ScienceOn
6 Ariizumi T., K. Sawamura, H. Uchiyama and M. Asashima, 1991, Dose and time-dependent mesoderm induction and outgrowth formation by activin A in Xenopus laevis. Int. J. Dev. BioI., 35, 407-414
7 Miyanaga Y., R Shiurba, S. Nagata, C. J. Pfeiffer and M. Asashima, 1998, Induction of blood ceIIs in Xenopus embryo explants, Dev. Genes EvoI., 207(7), 417-426   DOI   ScienceOn
8 Lechner W. D. and M. S. Abdel-Rahman, 1986, Kinetics of carbaryl and malation in combination in the rat, J. ToxicoI. Environ. Health 18(2), 241-256   DOI   ScienceOn
9 Vismara C., R. Bacchetta, B. Cacciatore, G. Vailati and U. Fascio, 2001, Paraquat embryotoxicity in the Xenopus laevis cleavage phase, Aquat ToxicoI., 55, 85-93   DOI   ScienceOn
10 Bonfantia P., A. Colomboa, F. Orsia, I. Nizzettoa, M. Andriolettib, R. Bacchetta, P. Mantecca, U. Fascioc, G. Vailati and C. Vismara, 2004, Comparative teratogenicity of chlorpyrifos and malathion on Xenopus laevis development, Aquat. ToxicoI. 70(3), 189-200   DOI   ScienceOn
11 Tardin D., 1972, Ultrastructural features of neural induction in Xenopus laevis, J. Anat., 111, 1-4
12 Hauptman O., D. M. Albert, M. C. Plowman, S. M. Hopfer and F. W. Sunderman Jr., 1993, Ocular malformation of Xenopus laevis exposed to nickel during embryogenesis, Ann. Clin. Lab. Sci., 23(6), 397-406
13 Vismara C., R. Bacchetta, B. Cacciatore, G. Vailati and U. Fascio, 2001, Paraquat embryotoxicity in the Xenopus laevis cleavage phase, Aquat. ToxicoI., 55, 85-93   DOI   ScienceOn
14 Mann R. M. and J. R. Bidwell, 2001, The acute toxicity of agricultural surfactants to the tadpoles of four Australian and two exotic frogs, Environ. PoIIut., 114(2), 195-205   DOI   ScienceOn
15 Bacchetta R, P. Mantecca, M. Andrioletti, C. Vismara and G. Vailati, 2007, Axial-skeletal defects caused by carbaryl in Xenopus laevis embryos, Sci. Total. Environ. 392, 110-118   DOI   ScienceOn
16 Lin C. C., M. N. Hui and S. H. Cheong, 2007, Toxicity and cardiac effects of carbaryl in early developing zebrafish(Danio rerio) embryos, Toxicology and Applied Pharmacology, 222, 159-168   DOI   ScienceOn
17 Moreno A. J., T. L. Serafim, P. J. Oliveira and V. M. Madeira, 2007, Inhibition of mitochondrial bioenergetics by carbaryl is only evident for higher concentrations - Relevance for carbaryl toxicity mechanisms, Chemosphere, 66(3), 404-411   DOI   ScienceOn
18 Yoon C. S., J. H. Jin, J. H. Park, H. J. Youn and S. W. Cheong, 2003, The fungicide benomyl inhibits differentiation of neural tissue in the Xenopus laevis embryo and animal cap explants, Environ. ToxicoI., 18, 327-224   DOI   ScienceOn
19 Luft J. H., 1963, Embedding media - old and new. In: Koehler JK, editor, Advanced techniques in biological electron microscopy, Springer-Verlag, 1-34
20 Reynold E. S., 1963, The use of lead citrate at high pH as an electron-opaque stain in electron microscopy, J. Cell Biol., 17, 208-212   DOI   ScienceOn
21 Hong S. U. and J. W. Lee, 1992, Toxic, effect of combination of buprofezin and carbaryl in rats, The Korean Society of Environmental Toxicology, 7(3-4), 17-35   과학기술학회마을
22 Flaskos J., M. J. Fowler, C. Teurtrie and A. J. Hargreaves, 1999, The effects of carbaryl and trichlorphan on differentiating mouse N2a neuroblastoma cells, Toxicol. Lett., 110(1-2), 79-84   DOI   ScienceOn
23 Bridges C. M., 2000, Long-term effects of pesticide exposure at various life stage of the Southern Leopard Frog (Rana sphenocephala), Arch. Environ. Contam. Toxicol., 39(1), 91-96   DOI   ScienceOn
24 Elliott-Feeley E. and J. B. Armstrong, 1981-1982, Effects of fenitrothion and carbaryl on Xenopus laevis development, Toxicology, 22(4), 319-335   DOI   ScienceOn
25 ASTM(American Society for Testing and Materials), 1998, Standard guide for conducting the frog embryo teratogenesis assay-Xenopus(FETAX), Philadelphia, 16pp
26 Nieuwkoop P. D. and J. Faber, 1956, Normal table of Xenopus laevis (Daudin), North-Holland Publishing Company, 243pp
27 Orkin S. H., F. l. Harosi and P. Leder, 1975, Differentiation in erythroleukernic cells and their somatic hybrids, Proc. Natl. Acad. Sci. USA., 72(1), 98-102   DOI   ScienceOn
28 Cooke J., J. C. Smith, E. J. Smith and M. Yaqoob, 1987, The organization of mesodermal pattern in Xenopus laevis: experiments using a Xenopus mesoderm-inducing factor, Development, 101, 893-908
29 Slack J. M. W., 1991, The nature of the mesoderm-inducing signal in Xenopus: A trans filter induction study, Development, 113, 661-669
30 농약공업협회, 2000, 농약사용지침서, 269pp
31 Tos-Luty S., D. Przebirowska, J. Latuszynska and M. Tokarska-Rodak, 2001, Histological and ultrastructural studies of rats exposed to carbaryl, Ann. Agric. Environ. Med., 8(2), 137-144
32 Pilar H. M., H. M. paz and R Alvarez, 1993, The carbamate insecticide ZZ-Aphox induced structural changes of gills. live, gall·bladder, heart, and notochord of Rana perez; tadpoles, Arch. Environ. Contam. Toxicol., 25(2), 184-191
33 Alvarez R, M. P. Honrubia and M. P. Herraez, 1995, Skeletal malformations induced by the insecticides ZZ-Aphox and Folidol during larval development of Rana prerzi, Arch. Environ. Contam. Toxicol., 28(3), 349-356
34 Lee T. K. and S. U. Hong, 1985, Toxicological study of carbaryl in rats, Arch. Pharmacal. Research, 8(3), 119-132   DOI