• Journal of Biosystems Engineering
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• v.43 no.1
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• pp.1-13
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• 2018

Purpose: The development of compact tractors that can be used in dry fields, greenhouses, and orchards for pest control, weeding, transportation, and harvesting is necessary. The development and performance evaluation of power transmission units are very important when it comes to tractor development. This study evaluates the performance of a driving power transmission unit of a 50 kW multi-purpose narrow tractor. Methods: The performance of the transmission and forward-reverse clutch, which are the main components of the driving power transmission unit of multi-purpose narrow tractors, was evaluated herein. The transmission performance was evaluated in terms of power transmission efficiency, noise, and axle load, while the forward-reverse clutch performance was evaluated in terms of durability. The transmission's power transmission efficiency accounts for the measurement of transmission losses, which occur in the transmission's gear, bearing, and oil seal. The motor's power was input in the transmission's input shaft. The rotational speed and torque were measured in the final output shaft. The noise was measured at each speed level after installing a microphone on the left, right, and upper sides. The axle load test was performed through a continuous equilibrium load test, in which a constant load was continuously applied. The forward-reverse clutch performance was calculated using the engine torque to axle torque ratio with the assembled engine and transmission. Results: The loss of power in the transmission efficiency test of the driving power unit was 6.0-9.7 kW based on all gear steps. This loss of horsepower was equal to 11-18% of the input power (52 kW). The transmission efficiency of the driving power unit was 81.5-89.0%. The noise of the driving power unit was 50-57 dB at 800 rpm, 70-77 dB at 1600 rpm, and 76-83 dB at 2400 rpm. The axle load test verified that the input torque and axle revolutions were constant. The results of the forward-reverse clutch performance test revealed that hydraulic pressure and torque changes were stably maintained when moving forward or backward, and its operation met the hydraulic design standards. Conclusions: When comprehensively examined, these research results were similar to the main driving power transmission systems from USA and Japan in terms of performance. Based on these results, tractor prototypes are expected to be created and supplied to farmhouses after going through sufficient in-situ adaptability tests.

• Korean journal of applied entomology
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• v.56 no.1
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• pp.19-28
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• 2017

Polydnaviruses (PDVs) are a group of double-stranded DNA viruses symbiotic to some endoparasitoid wasps. Cotesia plutellae bracovirus (CpBV) is a PDV symbiotic to an endoparasitoid wasp, C. plutellae, parasitizing young larvae of Plutella xylostella. An early expressed gene, CpBV-ELP1, plays an important role in the parasitism by suppressing host cellular immunity by its cytotoxic activity against hemocytes. This study aimed to test its oral toxicity against insect pest by expressing it in a recombinant tobacco plant. A recombinant CpBV-ELP1 protein was produced using a baculovirus expression system and secreted to cell culture medium. The cell cultured media were used to purify CpBV-ELP1 by a sequential array of purification steps: ammonium sulfate fractionation, size exclusion chromatography, and ion exchange chromatography. Purified rCpBV-ELP1 exhibited a significant cytotoxicity against Spodoptera exigua hemocytes. CpBV-ELP1 was highly toxic to the fifth instar larvae of S. exigua by injection to hemocoel. It also showed a significant oral toxicity to fifth instar larvae of S. exigua by a leaf-dipping assay. CpBV-ELP1 was cloned into pBI121 vector under CaMV 35S promoter with opaline synthase terminator. Resulting recombinant vector (pBI121-ELP1) was used to transform Agrobacterium tumefaciens LBA4404. The recombinant bacteria were then used to induce callus of a tobacco (Nicotiana tabacum Xanthi) leaves and subsequent generation (T1) plants were selected. T1 generation tobacco plants expressing CpBV-ELP1 gave significant insecticidal activities against S. exigua larvae. These results suggest that CpBV-ELP1 gene can be used to control insect pests by constructing transgenic crops.

• Korean journal of applied entomology
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• v.53 no.4
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• pp.331-338
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• 2014

Cystatins (CSTs) are reversible and competitive inhibitors of C1A cysteine proteases, corresponding to papain-like cathepsins in plants and animals. A viral CST (CpBV-CST1) was identified from a polydnavirus, Cotesia plutellae bracovirus (CpBV). Our previous study indicated that a transient expression of CpBV-CST1 interfered with immune response and development of Plutella xylostella larvae. To directly demonstrate the protein function, this study produced a recombinant CpBV-CST1 protein (rCpBV-CST1) using bacterial expression system to determine its inhibitory activity against cysteine protease and to assess its physiological alteration in insect immune and development. The open reading frame of CpBV-CST1 encodes a polypeptide of 138 amino acids (${\approx}15kDa$). rCpBV-cystatin protein in BL21 STAR (DE3) competent cells containing a recombinant pGEX4T-3:CpBV-CST1 was over-expressed by 0.5 mM IPTG for 4 h. In biological activity assay, the purified rCpBV-CST1 showed a significant inhibition against papain activity. It inhibited a cellular immune response of hemocyte nodule formation in the beet armyworm, Spodoptera exigua. Moreover, its oral administration retarded larval development of the diamondback moth, Plutella xylostella in a dose-dependent manner. These results suggest that CpBV-CST1 may be applied to control insect pest populations.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.215-220
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• 2021

To establish the systematic biological control system for various insect pests in strawberry under plastic houses, we were investigated the biological traits of lesser strawberry aphid, Chaetosiphon minus (Forbes), as a target pest. The lesser strawberry aphid is adapted to low temperatures while examining the availability of and selecting useful natural enemies. The development, survivorship and reproduction of lesser strawberry aphid were evaluated at four constant temperatures (10, 15, 20, and 25℃). The developmental periods of the nymphal stages ranged from 41.7 d at 10℃ to 9.8 d at 25℃. The developmental threshold temperature and degree day of nymphal stages are 5.5℃ and 185 DD. The reproduction rate (R₀) was higher at 20℃ (30.16) than at 25℃ (22.38). The 50 % survival rate and maximum longevity of adult females were 31 d and 59 d at 20℃, and 25 d and 36 d at 25℃, respectively. The average progeny per female was 35 at 20℃, and 26 at 25℃. We confirmed that compared to other strawberry aphids under plastic houses, the lesser strawberry aphid is more adapted to lower temperatures. It is, therefore, necessary to commercialize natural enemies such as syrphid flies with high activity at low temperatures.

• Korean journal of applied entomology
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• v.60 no.1
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• pp.135-143
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• 2021

Aerospace and geospatial technologies have become more accessible by researchers and agricultural practitioners, and these technologies can play a pivotal role in transforming current pest management practices in agriculture and forestry. During the past 20 years, technologies including satellites, manned and unmanned aircraft, spectral sensors, information systems, and autonomous field equipment, have been used to detect pests and apply control measures site-specifically. Despite the availability of aerospace and geospatial technologies, along with big-data-driven artificial intelligence, applications of such technologies to apple IPM have not been realized yet. Using a case study conducted at the Korea Apple Research Institute, this article discusses the advances and limitations of current aerospace and geospatial technologies that can be used for improving apple IPM.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.5
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• pp.383-393
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• 1991

Lodging of rice plant is the most important damage by unfavourable weather conditions in rice cultivation. High levels of nitrogen application and growing of Japonica rice variety is one factor to increase the lodging damage. Lodging of rice plant decreasing grain yield; 34% at milky. 21% at dough. 20% at yellow stage. decreasing grain Quality. increasing green rice. and increasing labor cost to harvest. To decrease lodging damage. the lodging resistant varieties will be selected and cultural practices such as amount and time of nitrogen application. planting density. water management. and disease and pest control methods have to be study for the short and strong culm. and good rooting system. Also. application methods such amount and time of plant growth regulators and new types of chemicals will be develop for the reducing lodging of rice plant. To decrease the lodging damage in direct seeding cultivation. first identifying the differences of lodging mechanisms between hand transplanting and direct seeding, second establish the suitable direct seeding methods such as seedling establishement. fertilization. and water menagement.

• Korean journal of applied entomology
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• v.61 no.3
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• pp.481-496
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• 2022

In order to establish an effective pest control strategy, we investigated the occurrence of lepidopteran insects in the major trees growing in urban forests from 2019 to 2021. In total, we recorded 76 species of 14 families in Prunus spp., 40 species of eight families in Acer spp., 43 species of 10 families in Zelkova serrata, 28 species of seven families in Rhododendron spp., and 10 species of four families in Hibiscus syriacus. Among these, Hyphantria cunea and Lymantria dispar were recorded from all five tree taxa monitored. The most frequently occurring species in the different tree groups were as follows: H. cunea and Phalera flavescens in Prunus yedoensis, Epinotia ulmicola in Z. serrata, and Rehimena surusalis in H. syriacus. H. cunea also occurred in Rhododendron spp. and Acer spp., although the numbers were lower than those recorded in Prunus spp. and Z. serrata. Our results highlight the importance of focusing on the frequency of occurrence of lepidopteran insects in Prunus spp. and Z. serrata, whereas the presence of these insects in both Acer spp. and Rhododendron spp. Appears to be less problematic. From the perspective of reducing the risks to human health, our findings indicate that the application of pheromones for monitoring lepidopteran species and the use of mating disruptors would be desirable approaches that would contribute to minimizing the spraying of pesticides in urban forests.

• Korean journal of food and cookery science
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• v.21 no.3 s.87
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• pp.290-300
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• 2005

Increased sanitation management of foodservice establishments is required because most of the reported foodborne-disease outbreaks were in the foodservice industry. The purpose of this study was to determine the important control points for good sanitation. In this study, we inspected twenty foodservice establishments in Seoul, Kyunggi, Kyungnam with a self-developed monitoring tool. These foodservice establishments included secondary schools, universities, and industries. Six of them had appointed as the HACCP-certified establishments from the Korea Food and Drug Administration. The inspection was conducted from June to August in 2002. The inspection tool consisted of nine dimensions and sixty-five items. The dimensions were 'personal sanitation', 'supply of raw food', 'food storage', 'handling of raw food and ready-to-eat', 'cleaning and sterilization', 'waste control', 'pest control', and 'control of establishment and equipment' The highest possible score of this inspection tool is 105 points. Statistical data analysis was completed using the SPSS Package(11.0) for descriptive analysis Kruskal-Wallis. The score for the secondary schools (83.6 points) was higher than for the others and number of in compliance item was 50.9 on average. Therefore, we concluded that the secondary schools' sanitation condition was good. The foodservice establishments acquired HACCP certification was 89.7 points, which was significantly higher than that of establishments not applying foodservices in total score. Instituting the HACCP system in a foodservice is very effective for sanitation management. Many out of the compliance observations were found in the dimensions of 'waste control', 'control of establishment and equipment', and 'supply of raw food' 'Clean condition of refrigerator' item was $65\%$ out of the compliance that was the highest percent in this study. 'Notify and observance of heating/reheating temperature' was $45\%$ out of compliance. Items which were over $30\%$ out of compliance were 'sterilization of knifes and chopping boards in cooking', 'education of workers', 'maintain refrigerator temperature blow $5^{\circ}C$', and 'countermeasure of infection workers' In the results, most of the foodservice establishments were poorly managed in temperature control and cross-contamination. The important control points revealed in this study were preventing contamination, cooking temperature compliance, management of raw food and refrigerator. Therefore foodservice establishments should pay attention to education and training about important control points. The systematic sanitation management monitoring tool developed in this study can be effectively applied for conducting self-inspection and improving the sanitary conditions of their own foodservice operations.

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.7-7
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• 2023

Currently, the main technologies of various fourth industries are big data, the Internet of Things, artificial intelligence, blockchain, mixed reality (MR), and drones. In particular, "digital twin," which has recently become a global technological trend, is a concept of a virtual model that is expressed equally in physical objects and computers. By creating and simulating a Digital twin of software-virtualized assets instead of real physical assets, accurate information about the characteristics of real farming (current state, agricultural productivity, agricultural work scenarios, etc.) can be obtained. This study aims to streamline agricultural work through automatic water management, remote growth forecasting, drone control, and pest forecasting through the operation of an integrated control system by constructing digital twin data on the main production area of the nojinot industry and designing and building a smart farm complex. In addition, it aims to distribute digital environmental control agriculture in Korea that can reduce labor and improve crop productivity by minimizing environmental load through the use of appropriate amounts of fertilizers and pesticides through big data analysis. These open-field agricultural technologies can reduce labor through digital farming and cultivation management, optimize water use and prevent soil pollution in preparation for climate change, and quantitative growth management of open-field crops by securing digital data for the national cultivation environment. It is also a way to directly implement carbon-neutral RED++ activities by improving agricultural productivity. The analysis and prediction of growth status through the acquisition of the acquired high-precision and high-definition image-based crop growth data are very effective in digital farming work management. The Southern Crop Department of the National Institute of Food Science conducted research and development on various types of open-field agricultural smart farms such as underground point and underground drainage. In particular, from this year, commercialization is underway in earnest through the establishment of smart farm facilities and technology distribution for agricultural technology complexes across the country. In this study, we would like to describe the case of establishing the agricultural field that combines digital twin technology and open-field agricultural smart farm technology and future utilization plans.

• Korean journal of applied entomology
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• v.56 no.3
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• pp.301-308
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• 2017

Locusts, Locusta migratoria (Orthoptera: Acrididae) are periodical unpredictable agricultural pests worldwide and cause serious damage to crop production; however, little consideration has been given to the management of this pest. Herein, we constructed a locust-pathogenic fungal library and confirmed that some fungi could be used as resources for locust management. First, the entomopathogenic fungi were collected from sampled soils using a Tenebrio molitor-based baiting system. For the locust assay, a locust colony was obtained from the National Institute of Agricultural Science and Technology. A total of 34 entomopathogenic fungal granules, which were produced by solid cultures, were placed in the plastic insect-rearing boxes (2 g/box) and nymphs of locust were contained in the box. In 3-7 days, mycosis was observed on the membranous cuticles of the head, abdomen, and legs of locusts. In particular, Metarhizium anisopliae, M. lepidiotae, and Clonostachys rogersoniana exhibited high virulence against the locust. Given that the 34 isolates could be used in field applications, their conidial production and stability (thermotolerance) were further characterized. In the thermotolerance assay, Paecilomyces and Purpureocillium isolates had higher thermotolerance than the other isolates. Most of the fungal isolates produced ca. >$1{\times}10^8conidia/g$ on millet grain medium. In a greenhouse trial, the granular application of M. anisopliae isolate on the soil surface resulted in 85.7% control efficacy. This work suggests that entomopathogenic fungi in a granular form can be effectively used to control the migratory locust.