• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.3
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• pp.73-77
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• 2004

The purpose of this study was to improve the ornamental value of Perilla frutescens var. acuta. The growth of Perilla frutescens var. acuta was significantly varied as according to light intensities. The plant height, crown width, petiole length, leaf length, leaf width, stem diameter, and chlorophyll content were the greatest with 30% shade treatment. All of growth characteristics decreased as increasing shading levels. The anthocyanin contents also decreased with 70% shading level. The leaf color turned from dark purplish red to deep yellowish green, and the growth rate and ornamental value were the lowest with 70% shading condition.

• Korean Journal of Breeding Science
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• v.40 no.4
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• pp.361-370
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• 2008

In order to better understand the morphological differentiation of the two cultivated types of Perilla crop and their weedy types in Korea and Japan, we studied the variation of 62 accessions by examining 15 morphological characteristics. By using ANOVA (one-way analysis of variance), we determined that var. frutescens and var. crispa showed significant morphological differences in terms of plant height and seed weight. Furthermore, cultivated var. frutescens and var. crispa could also be clearly discriminated from one another using PCA (principal component analysis). Specifically, quantitative and qualitative characteristics such as plant height, seed weight, degree of pubescence, shape of leaf, color of leaf, fragrance of plant, color of flower, color of stem and seed size greatly contributed to differences seen in the positive and negative direction on the first axis. In our study, most accessions of cultivated var. frutescens and those of its weedy type could be clearly discriminated from one another, however, most accessions of cultivated and weedy types of var. crispa were not clearly discriminated by the ANOVA and PCA analyses. These results indicated that cultivated var. frutescens can be considered to be a domesticated form, while the cultivated var. crispa can not be considered to be a domesticated form in Korea and Japan. It is our belief that our results concerning the morphological variations among cultivated types of Perilla crop and their weedy types in Korea and Japan will help ensure the long-term success of breeding programs and maximize the use of the germplasm resources in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.48-56
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• 2018

The present study aimed to understand the variation in seed germination according to the storage period after harvest in the accessions of cultivated and weedy types of Perilla species in Korea. In this milieu, we investigated the germination rate and germination energy of 59 Perilla accessions (15 cultivated var. frutescens type I, 4 cultivated var. frutescens type II, 20 weedy var. frutescens, and 20 weedy var. crispa), which were harvested in autumn 2016. The accessions of cultivated var. frutescens (type I) showed an average germination rate of 80.8% 6 months after harvest; however, the accessions of weedy var. frutescens and var. crispa showed an average germination rate of 0.9% and 8.7%, respectively. Furthermore, the accessions of cultivated var. frutescens (type II) showed an average germination rate of 18.2%. The accessions of cultivated var. frutescens (type I) showed an average germination energy of 75.8%, while the accessions of weedy var. frutescens and var. crispa showed an average germination energy of 0.6% and 6.9%, respectively. In addition, the accessions of cultivated var. frutescens (type II) showed an average germination energy of 14.3%. The germination rate and germination energy for the accessions of cultivated and weedy types of Perilla species increased marginally at 2 months from the first month after harvest. However, it did not significantly increase until six months after that. According to our results, there are two types of cultivated var. frutescens, namely, type I, which showed high germination rate and germination energy, and type II, which showed low germination rate and germination energy. The results of this study will provide basic information to understand variations in the germination of seeds during 6 months of storage period after harvest in the accessions of cultivated and weedy types of Perilla species in Korea.

• Food Science and Preservation
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• v.6 no.2
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• pp.239-244
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• 1999

Antimicrobial activity to the extracts of Perilla frutescens Briton var. acuta Kudo was investigated against various foodborne pathogenes or food poisioning microorganisms(Aspergillus flavus KCTC 6143 and KCTC 6961, Aspergillus niger ATCC 4695, Listeria monocytogenes ATCC 15313, Staphylococcus aureus 196E ATCC 13565, Escherichia coli O157:H7 ATCC 43895, Salmonella typhimurium ATCC 13311 and Yersinia enterocolitica). The ethanol extract of Perilla frutescens Briton var. acuta Kudo was very stable over heat at $121^{\circ}C$ for 15 min. In concentration of $1000\mu\textrm{g}$/mL into culture broth(TSB), the ethanol extract of Perilla frutescens Briton var. acuta Kudo showed the strongest antimicrobial activities against Listeria monocytogenes, followed by Staphylococcus aureus 196E, Salmonella typhimurium. Gram negative bacteria(Escherichia coli O157:H7, Salmonella 쇼phimurium, Yersinia enterocolitica) were less sensitive than Cram positive bacteria but the growth of Escherichia coli O157:H7 and Yersinia exterocolitica were inhibited with increasing concentrations of the extract in culture broth.

• Journal of Environmental Science International
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• v.5 no.2
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• pp.171-178
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• 1996

This experiment was performed with the purpose of finding out the effect of simulated acid rain at various pH levels on the morphology and enzyme of Perilla frutescens var. japonica hara. The pH levels of simulated acid rain ranged from pH 2.0 to pH 6.0. The experiment showed the anion concentrations in the order of $SO_4^{2-}$, Cl^-$, $NO_3^-$, and $F^-$, $SO_4^{-2}$ was found out to be the main factor which contributed to the rainwater acidification. A general decrease of growth in Perilla frutescens var. japonica hara growth was shown with the decreas of pH concentration. As acidity increases a definite reduction in the rates of germination, heigth of plant, malate dehydrogenase, and 6-phosphogluconate dehydrogenase was ovserved, but the density of spots on the leaf apex was increased.

• Fashion & Textile Research Journal
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• v.12 no.2
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• pp.240-245
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• 2010

The effects of monazite and fixing agents on cotton fabric dyed with Perilla frutescens var. acuta extract were investigated. The proper monazite treatment concentration, temperature and time were 10%(o.w.b.), $50^{\circ}C$ and 60minutes. By various fixing agent treatment, $FeSO_4$ showed a relatively high K/S value and the order of K/S value decreased as follows, cation surface active agents, soybean and NaCl. And the monazite and $FeSO_4$ fixing agent showed higher anion emissity than those of untreated cotton and other fixing agents. The cotton fabrics showed improved color fastness by monazite and fixing agents treatments with the exception of light fastness. And the cotton fabrics fixed with fixing agents were showed effective bacterial reduction with the exception of NaCl.

• Korean Journal of Pharmacognosy
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• v.43 no.2
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• pp.163-166
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• 2012

In the present study, we investigated the effect of the water extract of Perilla frutescens var. acuta (Labiatae; WEPF) on the mast cell-mediated allergic reactions. WEPF was anally administered to mice for high and fast absorption. WEPF inhibited compound 48/80-induced systemic allergic reaction. WEPF attenuated immunoglobulin E (IgE)-mediated local allergic reaction. In addition, WEPF decreased the gene expression of pro-inflammatory cytokines in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated HMC-1 cells. These results indicate that WEPF inhibits mast cell-mediated allergic reactions in vivo and in vitro.

• Natural Product Sciences
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• v.20 no.2
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• pp.71-75
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• 2014

Nine triterpenes were isolated from the petroleum ether and MeOH extract of Perilla frutescens var. acuta leaves. Their structures were determined to be arjunic acid (1), maslinic acid (2), oleanolic acid (3), euscaphic acid (4), tormentic acid (5), 3-O-trans-p-coumaroyltormentic acid (6), 28-formyloxy-$3{\beta}$-hydroxy-urs-12-ene (7), ursolic acid (8), and corosolic acid (9) by spectroscopic methods. The compounds 1, 2, 4, 6, and 7 were isolated for the first time from this plant and the Genus Labiatae. The isolated compounds (1-9) were tested for cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15) in vitro using a Sulforhodamin B bioassay.

• The Korea Journal of Herbology
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• v.21 no.2
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• pp.95-101
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• 2006

Objectives : This study aimed to establish cosmeceutical activities of Perilla frutescens var. acuta. Methods : Folium Perillae, which had been extracted, concentrated, and freeze drying with water and ethanol, have been used for the experiment. The effects on electronic donating ability, SOD-like activity, xanthine oxidase inhibition, whitening effect have been investigated in the cosmeceutical activity measurement of function experiment. Results : Both water and ethanol extracts of Perilla frutescens var. acuta showed relatively high electron donating ability of more than 70% at over 500ppm. Also, SOD like activity increased in a dose dependent matter of more than 80% at 5,000ppm, while tyrosinase showed insufficient inhibitory rate. Xanthine oxidase showed a meaningful inhibitory effect of 72.4% in water extract and 55.3% at 1,000ppm in ethanol extract. The addition of $Fe^{2+}ion\;and\;Cu^{2+}ion$ showed relatively high oxidizing inhibitory effect of 57% and 41%, respectively, at 500ppm in water extract and, when $Fe^{2+}$ ion was added in ethanol extract, the effect of 64% at 1,000ppm was achieved. Also, higher oxidizing inhibitory effect was shown against $Fe^{2+}$ rather than $Cu^{2+}$ in both water and ethanol extracts. For human cancer cells, relatively high growth inhibition ability was shown against melanoma G361 in both water and ethanol extracts. Conclusion : From the above results, it was confirmed that Perilla frutescens var. acuta could be used in functional cosmetics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.513-517
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• 2017

The purpose of this study was to investigate the antioxidative and anti-inflammatory activities of ethanol extracts from Perilla frutescens var. acuta by varying the concentration of ethanol at 30, 50, 70, and 90% to utilize the effective extract of Perilla frutescens as a cosmetic and pharmaceutical material. In the DPPH antioxidant activity test, the 70% ethanol extract showed the highest activity with an IC50 of 680.98ppm. ABTS showed a high activity in the 50% ethanol extract and the 70% ethanol extract with an IC50 of 646.94 and 661.94 ppm, respectively. Each ethanol extract showed antioxidant activity at a certain concentration (100-10000 ppm), but did not show any significant relationship with the ethanol extract concentration. In RAW 264.7 macrophages induced by LPS, each of the ethanol extracts showed reduced NO production in all extracts, and more than 50% ethanol extract (10000ppm) inhibited nitric oxide formation by 85% or more. In particular, the 70% ethanol extract showed 90% or more nitric oxide production inhibition. In addition, the MTT assay showed no cytotoxicity at all concentrations (1250-10000 ppm) of each extract. In this study, the ethanol extract of Perilla frutescens var. acuta has antioxidant activity and anti-inflammatory activity that is dependent on the concentration at each extraction concentration.