• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.111-111
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.08a
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• pp.200-201
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• 2005

• Korean Journal of Plant Resources
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• v.32 no.6
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• pp.743-751
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• 2019

Sclerotinia rot, caused by Sclerotinia sclerotiorum, is a devastating disease that poses a serious threat to perilla production in Korea. Identifying effective sources of resistance offers long term prospects for improving management of this disease. Screening disease resistant genetic resources is important for development of disease-resistant, new cultivars and conduct related research. In the present study, perilla germplasm were screened in vitro against S. sclerotiorum using detached leaf method. Among 544 perilla accessions, two were highly resistant (IT226504, IT226533), five were resistant (IT226561, IT226532, IT226526, IT226441, and IT226589), five were moderately resistant (IT226525, IT226640, IT226568, IT220624, and IT178655), 16 were moderately susceptible, 31 were susceptible, and 485 were highly susceptible. The resistant accessions in this study could serve as resistance donor in the breeding of Sclerotinia rot resistance or subjected to selection procedure of varietal development for direct use by breeders, farmers, researchers, and end consumers.

• Food Science and Biotechnology
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• v.18 no.3
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• pp.793-798
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• 2009

The objectives of this present study were to compare the contents and determine optimum extraction conditions for the rosmarinic acid (RA) and caffeic acid (CA) from leaves of Korean Perilla frutescens varieties. RA and CA from leaves of cv. Bora, a breeding line of P. frutescens were isolated and elucidated using various spectroscopic data. On the basis of 2 phenolic acids, optimum extraction conditions were obtained by employing 50% EtOH for 60 min at $25^{\circ}C$. We reported for the first time on the contents of RA and CA from leaves of 32 Korean varieties. Among them, leaves of P. frutescens Brit. var. acuta Kudo I exhibited the highest RA content ($8.53{\pm}0.57$ mg/g) and CA content ($2.33{\pm}0.11$ mg/g) showed the highest in the P. frutescens Brit. var. viridis Makino. Interestingly, average RA content ($2.66{\pm}0.17$ mg/g) showed a markedly higher than that of CA ($1.98{\pm}0.16$ mg/g) in Korean varieties. These results suggest that concentrations of the RA and CA in P. frutescens leaves could be a key factor in the selection process of a high quality species.

• Korean journal of food and cookery science
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• v.29 no.3
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• pp.241-248
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• 2013

This study was to compare the antioxidant and anti-proliferative activities of perilla (Perilla frutescens Britton) and sesame (Seasamum indicum L.) leaf extracts. The total polyphenol levels of sesame leaf ($634.7{\pm}1.2$ mg gallic acid equivalent/100 g dried leaf) were higher than those of perilla leaf ($408.7{\pm}4.6$ mg gallic acid equivalent/100 g dried leaf; p<0.001). The total flavonoid levels of sesame leaf ($166.7{\pm}17.3$ mg quercetin equivalent/100 g dried leaf) were also higher than those of perilla leaf ($108.2{\pm}3.7$ mg quercetin equivalent/100 g dried leaf; p<0.05). ABTS radical- and DPPH radical-scavenging activities of sesame leaf extracts (78.9% and 18.2%, respectively) were higher than those of perilla leaf extracts (46.0% and 9.0%, respectively; p<0.01). Both perilla and sesame leaf extracts significantly inhibited the growth of HCT116 human colon cancer cells. However, the inhibitory activities of sesame leaf extracts were more pronounced than those of perilla leaf extracts (p<0.001). These results indicate that sesame leaf extracts have higher antioxidant and anti-proliferative activities than perilla leaf extracts. More studies are needed in order to enhance the sensory value of sesame leaf and to develop sesame leaf as health/functional food ingredients.

• Journal of Plant Biotechnology
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• v.33 no.2
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• pp.111-115
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• 2006

This study was carried out to establish genetic transformation of Rot 3 gene into perilla plants and to evaluate aromatic compounds, brightness, anthocyanin contents and leaf index in Rot 3 overexpressing transgenic lines. Rot 3 transmitted successfully from T$_1$ to T$_2$ generation showing stable gene expression. It revealed that there was no difference between transgenic and non-transgenic plants in major agronomic characteristics of progeny analysis. There was not much difference in aromatic compounds and leaf brightness did not showed variations between transgenic and non-transgenic, but leaf index was distinguished, respectively.

• Nutrition Research and Practice
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• v.9 no.1
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• pp.11-16
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• 2015

BACKGROUND/OBJECTIVES: Perilla frutescens Britton leaves are a commonly consumed vegetable in different Asian countries including Korea. Cancer is a major cause of human death worldwide. The aim of the current study was to investigate the inhibitory effects of ethanol extract of perilla leaf (PLE) against important characteristics of cancer cells, including unrestricted growth, resisted apoptosis, and activated metastasis, using human cancer cells. MATERIALS/METHODS: Two human cancer cell lines were used in this study, HCT116 colorectal carcinoma cells and H1299 non-small cell lung carcinoma cells. Assays using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide were performed for measurement of cell growth. Soft agar and wound healing assays were performed to determine colony formation and cell migration, respectively. Nuclear staining and cell cycle analysis were performed for assessment of apoptosis. Fibronectin-coated plates were used to determine cell adhesion. RESULTS: Treatment of HCT116 and H1299 cells with PLE resulted in dose-dependent inhibition of growth by 52-92% (at the concentrations of 87.5, 175, and $350{\mu}g/ml$) and completely abolished the colony formation in soft agar (at the concentration of $350{\mu}g/ml$). Treatment with PLE at the $350{\mu}g/ml$ concentration resulted in change of the nucleus morphology and significantly increased sub-G1 cell population in both cells, indicating its apoptosis-inducing activity. PLE at the concentration range of 87.5 to $350{\mu}g/ml$ was also effective in inhibiting the migration of H1299 cells (by 52-58%) and adhesion of both HCT116 and H1299 cells (by 25-46%). CONCLUSIONS: These results indicate that PLE exerts anti-cancer activities against colon and lung cancers in vitro. Further studies are needed in order to determine whether similar effects are reproduced in vivo.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.293-301
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• 2013

This study was carried out to investigate the pesticide residue pattern among different leafy vegetables applied with foliar spraying under greenhouse and to check extrapolating from some residue trial data to other minor crops. Leafy vegetables used in this study were: Mustard greens (Brassica juncea L.), Kale (Brassica oleracea L.), Dacheongchae (a kind of pak-choi (Brassica rapa subsp. chinensis L.)), Leaf broccoli (Brassica oleracea var alboglabra), Perilla leaf (Perilla frutescens (L.) Britton var. Frutescens), Leaf lettuce (Lactuca sativa L.), Swiss chard (Beta vulgaris L. subsp. vulgaris) and Red leaf chicory (Cichorium intybus L. var. foliosum Hegi). These are cultivated all year under indoor or outdoor and cut the leaf from plant continuously during harvest time. The amounts of pesticide deposit in/on the continuous harvesting leafy vegetables were affected by the ratios of leaf area to weight. Ratio of perilla leaf was the largest among crops as 58 $cm^2/g$. The residue levels of 7 pesticides in/on perilla leaf were the highest than those of other crops through the statistical analysis from zero day to fifth day after last application. The representative crop in 8 crops was perilla leaf selected based on the amounts of daily consumption and the high residues. This study suggest that the continuous harvesting leafy greens should be separated from the one time harvesting leafy vegetables for the pesticide recommendations because of different harvesting habits and pre-harvest intervals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.408-415
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• 2013

To better understand the morphological variation of the Perilla crop and their weedy types in East and Southeast Asia, we studied the morphological variation of 90 accessions by examining 10 morphological characteristics, such as flowering time, seed size, seed hardness, seed color, color of surface leaf, color of reverse side leaf etc. As a result, morphological variation determined that between cultivated var. frutescens and var. crispa, and between cultivated var. frutescens and its weedy type showed significant morphological differences in terms of seed size and seed hardness, whenever cultivated var. crispa and its weedy type could not showed significant differences in most morphological characters. In PCAs (principal component analysis), among 10 morphological characteristics, flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), seed color (QL2), stem color (QL7), and color of reverse side leaf (QL4) contributed in negative direction on the first axis, while flowering time (QN1), leaf shape (QL5), and degree of pubescence (QL8) contributed in positive direction on the first axis. Among these morphological characters, particularly flower color (QL6), color of surface leaf (QL3), seed size (QN2), seed hardness (QL1), and degree of pubescence (QL8) were useful characters for discrimination between cultivated var. frutescens and weedy var. crispa, and between cultivated var. frutescens and its weedy type. However, most accession of cultivated and weedy types of var. crispa was not clearly discriminated by PCA analyses. Although the wild ancestral species of var. frutescens and of var. crispa are still unknown in East and Southeast Asia, the weedy types of Perilla crop may be the key taxon for our understanding of the origin of cultivated types of var. frutescens and var. crispa.

• Korean Journal of Environmental Agriculture
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• v.7 no.1
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• pp.43-51
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• 1988

$SO_2$ gas injuries for 19 varieties of 10 species of plants were investigated within a environmentally controlled growth chamber. Visible injuries were observed when exposing the plants either 0, 0.2, 0.4, 0.7 or 1.5ppm of $SO_2$ gas for 5 days from 9 : 00 to 17 : 00 everyday. The results obtained were as follows: 1. Light brown spots were shown on Raphanus sativus(Chunchu, Taeback, Jinjudaepyung), Brassica campestris(Manchun, Josaengmiho, Seoul, Jeonseung), and Capsicum annum(Searona, Hongsanho, Hongilpum); brown spots on Sesamum indicum(Pungnyun, Kwangsan); reddish brown shades on Cleosia cristata; and black brown spots on Perilla frutescens, Calendula officinalis, Chrysanthemum morifolium, and Salvia splendens, respectively. 2. The spotty injuries induced by $SO_2$ gas injuries for 19 varieties of 10 species of plants were investigated within a environmentally controlled growth chamber. Visible injuries were observed when exposing the plants either 0, 0.2, 0.4, 0.7 or 1.5ppm of $SO_2$ gas for 5 days from 9 : 00 to 17 : 00 everyday. The results obtained were as follows: 1. Light brown spots were shown on Raphanus sativus(Chunchu, Taeback, Jinjudaepyung), Brassica campestris(Manchun, Josaengmiho, Seoul, Jeonseung), and Capsicum annum(Searona, Hongsanho, Hongilpum); brown spots on Sesamum indicum(Pungnyun, Kwangsan); reddish brown shades on Cleosia cristata; and black brown spots on Perilla frutescens, Calendula officinalis, Chrysanthemum morifolium, and Salvia splendens, respectively. 2. The spotty injuries induced by SO₂ gas on Raphanus sativus, Callistephus chinensis, Capsicum annum, Perilla frutescens, Calendula officinalis, Salvia splendens, and Sesamum indicum; the many small spots on Chrysanthemum morifolium; and the brown shade on Celosia cristata appeared on the upper surface of the intervein, on the leaf apex area and on the entire upper surface of leaves, respectively. 3. Visual injuries of Capsicum annum(Chunchu), Perilla frutescens, Sesamum indicum(Pungnyun, Kwangsan), and Salvia splendens were developed at 0.4ppm of SO₂ gas fumigation. Brassica campestris(Jeonseung), Capsicum annum(Searona, Hongilpum), and Cleosia cristata, however, showed the injury at 1.5ppm of $SO_2$ gas concentration. 4. Based on the tolerance grade(time when the 1st injury appeared), Raphanus sativus, Perilla frutescens, Sesamum indicum, and Salvia splendens were the most sensitive plants and Chrysanthemum morifolium, Callistephus chinensis, Cleosia cristata, and Calendula officinalis were the plants most tolerant of $SO_2$ gas. gas on Raphanus sativus, Callistephus chinensis, Capsicum annum, Perilla frutescens, Calendula officinalis, Salvia splendens, and Sesamum indicum; the many small spots on Chrysanthemum morifolium; and the brown shade on Celosia cristata appeared on the upper surface of the intervein, on the leaf apex area and on the entire upper surface of leaves, respectively. 3. Visual injuries of Capsicum annum(Chunchu), Perilla frutescens, Sesamum indicum(Pungnyun, Kwangsan), and Salvia splendens were developed at 0.4ppm of $SO_2$ gas injuries for 19 varieties of 10 species of plants were investigated within a environmentally controlled growth chamber. Visible injuries were observed when exposing the plants either 0, 0.2, 0.4, 0.7 or 1.5ppm of $SO_2$ gas for 5 days from 9 : 00 to 17 : 00 everyday. The results obtained were as follows: 1. Light brown spots were shown on Raphanus sativus(Chunchu, Taeback, Jinjudaepyung), Brassica campestris(Manchun, Josaengmiho, Seoul, Jeonseung), and Capsicum annum(Searona, Hongsanho, Hongilpum); brown spots on Sesamum indicum(Pungnyun, Kwangsan); reddish brown shades on Cleosia cristata; and black brown spots on Perilla frutescens, Calendula officinalis, Chrysanthemum morifolium, and Salvia splendens, respectively. 2. The spotty injuries induced by SO₂ gas on Raphanus sativus, Callistephus chinensis, Capsicum annum, Perilla frutescens, Calendula officinalis, Salvia splendens, and Sesamum indicum; the many small spots on Chrysanthemum morifolium; and the brown shade on Celosia cristata appeared on the upper surface of the intervein, on the leaf apex area and on the entire upper surface of leaves, respectively. 3. Visual injuries of Capsicum annum(Chunchu), Perilla frutescens, Sesamum indicum(Pungnyun, Kwangsan), and Salvia splendens were developed at 0.4ppm of SO₂ gas fumigation. Brassica campestris(Jeonseung), Capsicum annum(Searona, Hongilpum), and Cleosia cristata, however, showed the injury at 1.5ppm of $SO_2$ gas concentration. 4. Based on the tolerance grade(time when the 1st injury appeared), Raphanus sativus, Perilla frutescens, Sesamum indicum, and Salvia splendens were the most sensitive plants and Chrysanthemum morifolium, Callistephus chinensis, Cleosia cristata, and Calendula officinalis were the plants most tolerant of $SO_2$ gas. gas fumigation. Brassica campestris(Jeonseung), Capsicum annum(Searona, Hongilpum), and Cleosia cristata, however, showed the injury at 1.5ppm of $SO_2$ gas concentration. 4. Based on the tolerance grade(time when the 1st injury appeared), Raphanus sativus, Perilla frutescens, Sesamum indicum, and Salvia splendens were the most sensitive plants and Chrysanthemum morifolium, Callistephus chinensis, Cleosia cristata, and Calendula officinalis were the plants most tolerant of $SO_2$ gas.