• Title/Summary/Keyword: Performance increase

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Effects of the Castration Time on Growth Performance, Meat Quality and Fatty Acid Profiles of Korean Black Goats (흑염소의 거세시기가 발육, 육질 및 지방산조성에 미치는 영향)

  • Choi, Sun-Ho;Kim, Sang-Woo;HwangBo, Soon;Choe, Chang-Yong;Kim, Jin-Hyoung
    • Journal of Animal Science and Technology
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    • v.52 no.1
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    • pp.37-42
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    • 2010
  • A study was conducted to examine the effect of the castration time on growth, meat quality and fatty acid composition of Korean black goats. Forty five male kids were divided into five groups, including control (without castration) and four groups with the kids castrated at 0.5, 3, 5 and 7 months of age, respectively. Average daily body weight gain (ADG) for control was significantly (P<0.05) higher than the castrated groups. ADG did not differ between the castrated groups. The ADG of the male kids castrated at 3 and 5 months of age tended to be higher than the other castrated groups. Dressing percentage for 0.5 months-castrated group was higher than 7 months-castrated group. Retail cut percentages for control was higher (P<0.05) than others, but the retail cut percentages did not differ between the castrated groups. Compared with control, higher fat percentages of carcasses appeared in the castrated groups (P<0.05). The fat percentages gradually increased in earlier castrated animals. As castrated earlier, moisture contents tended to decrease, whereas crude protein and fat contents tended to increase. The different castration time did not affect physical properties of goat meat (shear force, cooking loss, and water holding capacity). Results from panel tests showed that juiciness or tenderness of meats for 5 months-castrated group tended to be higher than those for the other groups. The flavor of meatfor 7-months castrated group appeared to be more favorable compared with 0.5- or 3-months castrated groups (P<0.05). The proportion of saturated fatty acid in meat washigher for 5-months castrated group and lower for 3-months castrated group as compared to the other castrated groups, whereas the proportion of unsaturated fatty acid was vice versa (P<0.05). Mono-unsaturated fatty acid contents did not differ between thecastrated groups. Present results indicatedthat castration at 3 or 5 months of age increased growth performance and meat quality of Korean black goats.

Effects of Dietary Chitosan Supplementation with Chitosan Feeding Levels and Feeding Forms on Growth Performance and Carcass Characteristics of Thigh Muscular in Broiler (키토산의 급여량과 급여 방법에 따른 육계의 성장 및 닭 다리육의 품질에 미치는 영향)

  • Kim, Y.J.;Kim, B.K.
    • Korean Journal of Poultry Science
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    • v.36 no.1
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    • pp.29-37
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    • 2009
  • This study was conducted to determine the effects of dietary supplementation with chitosan feeding levels and feeding forms on the performance, proximate composition, pH, thiobarbituric acid reactive substance (TBARS), water holding capacity (WHC), shear force, meat color, and fatty acid of chicken thigh meat. Two hundred broilers (Arbor Acre Broiler, male) were randomly assigned to five groups and were fed for five weeks and slaughtered. Thigh muscle was evaluated in this experiment. The amounts of proximate composition, crude fat of control and T3 for the chitosan-treated groups were significantly higher as compared with T2 (P<0.05), but no significant effects were detected on moisture, crude protein, and crude ash. By comparison, pH was significantly increased when chitosan was included at 2% into diet or more than 1% into drinking water. TBARS was significantly lower at chitosan treated groups it was decreased with increasing chitosan level in the diets (P<0.05). Therefore, chitosan had the possibility to improve shelf life of chicken meat. Higher chitosan levels and feeding additive increased high density lipoprotein cholesterol (HDL-C) and reduced total cholesterol and low density lipoprotein cholesterol (LDL-C) (P<0.05). $L^*$ and $a^*$ of chitosan treated group was higher than the control. As the dietary chitosan levels increase, the composition of palmitic acid and oleic acid levels was increased, however, those but the rates of linoleic acid and arachidonic acid were decreased. It is concluded that dietary chitosan has a positive effect on increasing HDL-C and oleic acid and decreasing total-C, LDL-C and TBARS values. Therefore, the treatment with the most significant effects in the current study was the high level of chitosan.

Studies on the Reproductive Performance and Treatment of Reproductive Disorder in Hanwoo (한우 번식우 농가의 번식실태 및 번식장애 치료에 관한 연구)

  • 김학영;송상현;조헌조
    • Korean Journal of Animal Reproduction
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    • v.26 no.3
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    • pp.291-298
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    • 2002
  • This study was carried out to investigate the reproductive status and the effect of progesterone treatment on the recovery of reproductive disorders in Hanwoo. Hanwoo farms were surveyed the general management status, such as the type of barn, the feed intake, the incidence of reproductive disorders. The reproductive disorder cattle were treated 7 days insertion of control internal drug releasing for cattle (CIDR) and injection of PGF$_2$$\alpha$ at 1 day before removal. The recovery of reproductive disorders was assessed by determining the pregnancy following artificial insemination. The total number of surveyed Hanwoo farms was 127, and the total incidence rate of reproductive disorders was 19.7% (209/1,061). Compared to the herd size, the incidence rates of reproductive disorders in less than 10 heads (37.5%) was significantly (P<0.05) higher than in 10 to 20 heads (14.7%) and more than 20 heads (13.6%) of herd size per farm. The incidence rate of reproductive disorders in tie stall barn was significantly (P<0.05) higher than in free stall barn (30.4% vs. 14.7%), and even in free stall barn, that tended to decrease as the floor area was larger. The incidence rate of reproductive disorder by the parity was highest in heifer (50.7%), and that tended to decrease as the parity was increased. The distribution rate of the case of reproductive disorder in anestrus, recovery rate of reproductive disorders fellowing CIDR treatment was 75.1% (157/209). In the recovery rate of reproductive disorders by body condition score (BCS), BCS 1, 2 and 3 was significantly (P<0.05) higher pregnancy rate (85.7, 84.9 and 86.8%), and gross recovery rate in emaciated cattle was better than in obese cattle (BCS 4, 5). In conclusion, the incidence rate of reproductive disorders in Hanwoo raised in Youngju province area was 19.7%, and that tended to decrease as the herd size was increase because of increased farmer's attentions, and the floor area was larger. The majority of the case of reproductive disorders was anestrus. The recovery rate of reproductive disorders following CIDR treatment was 75.1%, and to optimize the recovery rate of reproductive disorders, cows and heifers were maintained BCS 1, 2 and 3 by moderate management.

Glass Dissolution Rates From MCC-1 and Flow-Through Tests

  • Jeong, Seung-Young
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2004.06a
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    • pp.257-258
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    • 2004
  • The dose from radionuclides released from high-level radioactive waste (HLW) glasses as they corrode must be taken into account when assessing the performance of a disposal system. In the performance assessment (PA) calculations conducted for the proposed Yucca Mountain, Nevada, disposal system, the release of radionuclides is conservatively assumed to occur at the same rate the glass matrix dissolves. A simple model was developed to calculate the glass dissolution rate of HLW glasses in these PA calculations [1]. For the PA calculations that were conducted for Site Recommendation, it was necessary to identify ranges of parameter values that bounded the dissolution rates of the wide range of HLW glass compositions that will be disposed. The values and ranges of the model parameters for the pH and temperature dependencies were extracted from the results of SPFT, static leach tests, and Soxhlet tests available in the literature. Static leach tests were conducted with a range of glass compositions to measure values for the glass composition parameter. The glass dissolution rate depends on temperature, pH, and the compositions of the glass and solution, The dissolution rate is calculated using Eq. 1: $rate{\;}={\;}k_{o}10^{(ph){\eta})}{\cdot}e^{(-Ea/RT)}{\cdot}(1-Q/K){\;}+{\;}k_{long}$ where $k_{0},\;{\eta}$ and Eaare the parameters for glass composition, pH, $\eta$ and temperature dependence, respectively, and R is the gas constant. The term (1-Q/K) is the affinity term, where Q is the ion activity product of the solution and K is the pseudo-equilibrium constant for the glass. Values of the parameters $k_{0},\;{\eta}\;and\;E_{a}$ are the parameters for glass composition, pH, and temperature dependence, respectively, and R is the gas constant. The term (1-Q/C) is the affinity term, where Q is the ion activity product of the solution and K is the pseudo-equilibrium constant for the glass. Values of the parameters $k_0$, and Ea are determined under test conditions where the value of Q is maintained near zero, so that the value of the affinity term remains near 1. The dissolution rate under conditions in which the value of the affinity term is near 1 is referred to as the forward rate. This is the highest dissolution rate that can occur at a particular pH and temperature. The value of the parameter K is determined from experiments in which the value of the ion activity product approaches the value of K. This results in a decrease in the value of the affinity term and the dissolution rate. The highly dilute solutions required to measure the forward rate and extract values for $k_0$, $\eta$, and Ea can be maintained by conducting dynamic tests in which the test solution is removed from the reaction cell and replaced with fresh solution. In the single-pass flow-through (PFT) test method, this is done by continuously pumping the test solution through the reaction cell. Alternatively, static tests can be conducted with sufficient solution volume that the solution concentrations of dissolved glass components do not increase significantly during the test. Both the SPFT and static tests can ve conducted for a wide range of pH values and temperatures. Both static and SPFt tests have short-comings. the SPFT test requires analysis of several solutions (typically 6-10) at each of several flow rates to determine the glass dissolution rate at each pH and temperature. As will be shown, the rate measured in an SPFt test depends on the solution flow rate. The solutions in static tests will eventually become concentrated enough to affect the dissolution rate. In both the SPFt and static test methods. a compromise is required between the need to minimize the effects of dissolved components on the dissolution rate and the need to attain solution concentrations that are high enough to analyze. In the paper, we compare the results of static leach tests and SPFT tests conducted with simple 5-component glass to confirm the equivalence of SPFT tests and static tests conducted with pH buffer solutions. Tests were conducted over the range pH values that are most relevant for waste glass disssolution in a disposal system. The glass and temperature used in the tests were selected to allow direct comparison with SPFT tests conducted previously. The ability to measure parameter values with more than one test method and an understanding of how the rate measured in each test is affected by various test parameters provides added confidence to the measured values. The dissolution rate of a simple 5-component glass was measured at pH values of 6.2, 8.3, and 9.6 and $70^{\circ}C$ using static tests and single-pass flow-through (SPFT) tests. Similar rates were measured with the two methods. However, the measured rates are about 10X higher than the rates measured previously for a glass having the same composition using an SPFT test method. Differences are attributed to effects of the solution flow rate on the glass dissolution reate and how the specific surface area of crushed glass is estimated. This comparison indicates the need to standardize the SPFT test procedure.

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Electrochemical Properties of Pyrolytic Carbon and Boron-doped Carbon for Anode Materials In Li-ion Secondary Batteries (리튬 이온 이차전지 부극용 열분해 탄소 및 붕소첨가 탄소의 전기화학적 특성)

  • Kwon, Ik-Hyun;Song, Myoung-Youp;Bang, Eui-Yong;Han, Young-Soo;Kim, Ki-Tae;Lee, Jai-Young
    • Journal of the Korean Electrochemical Society
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    • v.5 no.1
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    • pp.30-38
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    • 2002
  • Disordered carbon and boron-substituted disordered carbons $C_{l-x}B_x(x=0.05,\;0.10,\;0.20)$ were synthesized by Pyrolysis of LPG(liquid Propane gas)and $BCl_3$. Their electrochemical properties as anode materials for Li-ion secondary batteries were then investigated. When PVDF is added to the sample in a weight ratio 5 : 95, the disordered carbon with x=0.00 had the first discharge capacity 374 mAh/g. Its cycling performance was relatively good from the second cycle and it had the discharge capacity 258 mAh/g at the 10th cycle. When PVDF is added to the sample in a weight ratio 5 : 95, the sample with x=0.05 among the samples $C_{l-x}B_x(x=0.05,\;0.10,\;0.20)$ exhibited the largest first discharge capacity 860 mAh/g and discharge capacity 181 mAh/g at the 10th cycle. All the samples had similar cycling performances from the second cycle. The sample $C_{0.90}B_{0.10}$ showed the best electrochemical properties as a anode materials fur Li-ion secondary battery from the view points of the first discharge capacity(853 mAh/g when $10w1.\%$ PVDF is used), cycling performance, discharge capacity(400mAh/g at the 10th cycle when $10wt.\%$ PVDF is used). All the samples showed generally larger charge and discharge capacities when $10wt.\%$ PVDF ratter than $5wt.\%$ PVDF is used. The plateau region in the range of voltage lower than 1.25V becomes larger probably since the structure becomes less disordered by the addition of boron. When boron is added, the charge and discharge capacities decreased suddenly at the second cycle. This may be become only a part of Li are reversibly deintercalated and intercalated and a part of Li which are strongly combined with B are not deintercalated. The increases in charge and discharge capacities are considered to be resulted from the increase in the potential of Li in the boron-added carbons, caused by the strengthening of the chemical bond between the intercalated Li and the boron-carbon host since the boron acts as electron acceptor.

Effect of Dietary Oligosaccharides on the Performance, Intestinal Microflora and Serum Immunoglobulin Contents in Laying Hens (Oligosaccharides 계열 생균제가 산란계의 생산성, 장내 미생물 및 혈청 면역 글로불린 함량에 미치는 영향)

  • Kim, C.H.;Shin, K.S.;Woo, K.C.;Paik, I.K.
    • Korean Journal of Poultry Science
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    • v.36 no.2
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    • pp.125-131
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    • 2009
  • This study was conducted to investigate the effects of dietary supplementation of mannan-oligosaccharides (MOS) and fructo-oligosaccharides (FOS) on the performance, immune response and small intestinal microflora in laying hens. A total of 960 Hy-Line $Brown^{(R)}$ laying hens of 27 wks old, housed in 2 bird cages, were assigned in a completely randomized block design into one of the following 6 dietary treatments: control, antibiotic (6 ppm avilamycine), 0.025% MOS, 0.05% MOS, 0.25% FOS, and 0.5% FOS. Each treatment had 4 replicates of 40 birds and was fed ad libitum for 6 wks under 16 h lighting regimen. There were significant differences among treatments in hen-day and hen-housed egg production. Hen-day egg production in 0.025% MOS was significantly higher than that of control. Hen-housed egg production in antibiotic-treated group was significantly higher compared with control. Egg weight, feed intake and feed conversion were not significantly different among treatments. Egg shell thickness was highest in 0.25% FOS, but was not significantly different among the rest of treatments. There were no significant differences among treatments in egg shell strength, egg shell color, egg yolk color and Haugh unit. IgG concentrations in serum were not significantly different among treatments. On the other hand, IgA concentrations of the treated birds tended to be increased compared with control. Dietary treatments tended to decrease Cl. perfringens and E. coli, and to increase Lactobacillus spp. The result of this experiment showed that dietary supplementation of MOS and FOS in laying hens tended to improve egg production comparable to the supplementation of antibiotics. The level of serum IgA and small intestinal microflora were also significantly affected by the treatments.

The Efficiency Analysis of CRM System in the Hotel Industry Using DEA (DEA를 이용한 호텔 관광 서비스 업계의 CRM 도입 효율성 분석)

  • Kim, Tai-Young;Seol, Kyung-Jin;Kwak, Young-Dai
    • Journal of Intelligence and Information Systems
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    • v.17 no.1
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    • pp.91-110
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    • 2011
  • This paper analyzes the cases where the hotels have increased their services and enhanced their work process through IT solutions to cope with computerization globalization. Also the cases have been studies where national hotels use the CRM solution internally to respond effectively to customers requests, increase customer analysis, and build marketing strategies. In particular, this study discusses the introduction of the CRM solutions and CRM sales business and marketing services using a process for utilizing the presumed, CRM by introducing effective DEA(Data Envelopment Analysis). First, the comparison has done regarding the relative efficiency of L Company with the CCR model, then compared L Company's restaurants and facilities' effectiveness through BCC model. L Company reached a conclusion that it is important to precisely create and manage sales data which are the preliminary data for CRM, and for that reason it made it possible to save sales data generated by POS system on each sales performance database. In order to do that, it newly established Oracle POS system and LORIS POS system concerned with restaurants for food and beverage as well as rooms, and made it possible to stably generate and manage sales data and manage. Moreover, it set up a composite database to control comprehensively the results of work processes during a specific period by collecting customer registration information and made it possible to systematically control the information on sales performances. By establishing a system which unifies database and managing it comprehensively, impeccability of data has been greatly enhanced and a problem which generated asymmetric data could be thoroughly solved. Using data accumulated on the comprehensive database, sales data can be analyzed, categorized, classified through data mining engine imbedded in Polaris CRM and the results can be organized on data mart to provide them in the form of CRM application data. By transforming original sales data into forms which are easy to handle and saving them on data mart separately, it enabled acquiring well-organized data with ease when engaging in various marketing operations, holding a morning meeting and working on decision-making. By using summarized data at data mart, it was possible to process marketing operations such as telemarketing, direct mailing, internet marketing service and service product developments for perceived customers; moreover, information on customer perceptions which is one of CRM's end-products could feed back into the comprehensive database. This research was undertaken to find out how effectively CRM has been employed by comparing and analyzing the management performance of each enterprise site and store after introducing CRM to Hotel enterprises using DEA technique. According to the research results, efficiency evaluation for each site was calculated through input and output factors to find out comparative CRM system usage efficiency of L's Company four sites; moreover, with regard to stores, the sizes of workforce and budget application show a huge difference and so does the each store efficiency. Furthermore, by using the DEA technique, it could assess which sites have comparatively high efficiency and which don't by comparing and evaluating hotel enterprises IT project outcomes such as CRM introduction using the CCR model for each site of the related enterprises. By using the BCC model, it could comparatively evaluate the outcome of CRM usage at each store of A site, which is representative of L Company, and as a result, it could figure out which stores maintain high efficiency in using CRM and which don't. It analyzed the cases of CRM introduction at L Company, which is a hotel enterprise, and precisely evaluated them through DEA. L Company analyzed the customer analysis system by introducing CRM and achieved to provide customers identified through client analysis data with one to one tailored services. Moreover, it could come up with a plan to differentiate the service for customers who revisit by assessing customer discernment rate. As tasks to be solved in the future, it is required to do research on the process analysis which can lead to a specific outcome such as increased sales volumes by carrying on test marketing, target marketing using CRM. Furthermore, it is also necessary to do research on efficiency evaluation in accordance with linkages between other IT solutions such as ERP and CRM system.

Effect of Dietary Supplementation of Cu-methionine Chelate and Cu-soy Proteinate on the Performance, Small Intestinal Microflora and Immune Response in Laying Hens (사료내 Cu-methionine Chelate와 Cu-soy Proteinate가 산란계의 생산성, 소장내 미생물 균총 및 면역체계에 미치는 영향)

  • Paik, I.K.;Kim, C.H.;Park, K.W.
    • Korean Journal of Poultry Science
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    • v.35 no.3
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    • pp.303-311
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    • 2008
  • This study was conducted to investigate the effect of dietary supplementation of Cu-methionine chelate(Cu-Met) and Cu-soy proteinate(Cu-SP) on the performance, small intestinal microflora and immune response in laying hens. A total of 960 Hy-line $Brown^{(R)}$ laying hens of 39 wks old were assigned to one of the following 6 dietary treatment: control(C), antibiotic(Avilamycine 6 ppm), Cu-Met 50 and Cu-Met 100(50 and 100 ppm Cu as Cu-methionine chelate), Cu-SP 50 and Cu-SP 100(50 and 100ppm Cu as Cu-soy proteinate). Each treatment was replicated 4 times with forty birds per replication, housed in 2 birds per cages. Forty birds units were arranged according to randomized block design. Feeding trial lasted 6 wks under 16 hours lighting regimen. Hen-day and hen-house egg production of groups treated with Antibiotic and Cu supplements tended to be higher than the control with significant difference (P<0.05) shown between Cu-Me 100 and control. Egg weight was significantly (P<0.05) heavier in antibiotic and Cu-SP treatments than Cu-Met treatments but they were not significantly different from the control. Eggshell strength, egg shell thickness, egg yolk color and Haugh unit were not significantly different among treatments. There were no significant differences in leukocytes and erythrocytes in the chicken blood. But mean corpuscular hemoglobin value(MCH) was significantly (P<0.05) higher in Cu-SP 100 than antibiotic treatment. The concentrations of serum IgG and IgA were not significantly different among treatments. Copper concentration in the liver tended to increase as the level of copper supplementation increased, that of Cu-SP 100 being significantly (P<0.05) higher than those of the control and antibiotic treatment. Concentrations of iron and zinc of the liver were not significantly influenced by treatments. Populations of Cl. perfringens and Lactobacilli in the small intestinal content were significantly (P<0.05) influenced by treatments. Population of Cl. perfringens decreased and that of Lactobacilli increased in the copper supplemented groups. The result of this experiment showed that Cu-Met and Cu-SP are comparable to antibiotic in improving egg production in laying hens. Birds fed diets supplemented with Cu-SP produced heavier eggs than those fed diets with Cu-Met. There were no significant differences in the performances between 50 ppm and 100 ppm copper supplementation as organic forms.

Effects of Production Performance, Immunity and Egg Quality by Raising on Exercise Yard in Laying Hens (산란계 운동장 사육이 생산성, 면역성 및 계란의 품질에 미치는 영향)

  • Kim, Ki Soo;Lee, Suk Kyung;Choi, Young Sun;Ha, Chang Ho;Kim, Won Ho
    • Korean Journal of Poultry Science
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    • v.40 no.2
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    • pp.97-103
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    • 2013
  • The present study examined effects of production performance, immune activity and egg quality by raising on exercise yard in laying Hens, the results of which could be used as baseline data to enhance animal welfare and the safety of livestock products. A total of 90 Hy-line-Brown laying hens of 13 weeks old were used in the experiment for 38 weeks. The cage group (Cage group) was raised in a cage, where an area of $0.084m^2$ was assigned to two hens, while hens in the exercise yard $1.1m^2$ group ($1.1m^2$ group) was assigned to a combination of a chicken house ($0.11m^2$) and a exercise yard ($1.1m^2$) per a hen. Hens in the exercise yard $2.2m^2$ group ($2.2m^2$ group) was assigned to a combination of a chicken house ($0.11m^2$) and a exercise yard ($2.2m^2$) per a hen. Treatment was replicated 3 times with ten birds per replication. Ten birds were arranged according to randomized block design. While initial egg production rate was significantly higher in the Cage group, $1.1m^2$ group exhibited a slightly higher rate in the mid- and late-stage of the experiment, although the difference was not statistically significant. Exercise yard treatment groups exhibited a higher feed intake rate than the Cage group up until the hens were 39 weeks old (P<0.05), but the difference dissipated from that age on. The age at first egg in the exercise yard treatment groups was 16 days later than that for the Cage group (P<0.05), although differences in the quality of the eggs were not observed. The results of immune activity test showed that IgA in the exercise yard treatment groups was significantly higher than that in the Cage group (P<0.05). IgG, IgM, and corticosterone were also higher in the exercise yard treatment groups. The soil in exercise yard increased of organic matter and decreased of cation exchange capacity (CEC) in raised hens. In sum, raising hens in a exercise yard raise style decreased the rate of initial egg production, which was followed by a slight increase during the latter part of the experiment. The exercise yard raise hens' immune activity was heightened.

The Effect of Early Chick Weight on Market-Weight in Korean Native Chickens (토종닭의 초기성장체중이 출하체중에 미치는 영향)

  • Kim, Ki Gon;Choi, Eun Sik;Kwon, Jae Hyun;Sohn, Sea Hwan
    • Korean Journal of Poultry Science
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    • v.44 no.4
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    • pp.259-265
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    • 2017
  • This study was to investigate the effect of chick weight in the early growth period on market-weight of Korean native chickens (KNCs). We measured the body weights of 1,087 chickens (male 479, female 608) of 13 KNC strains at 1-84 days of age at two week intervals. The growth performance of the 13 KNC strains was investigated. Correlation coefficients among the weights of chickens in each growth period and regression of market-weight on early chick weights were analyzed. The results showed that the average body weight of 70-day-old KNCs was 1,962g: 2,154g and 1,819.7g for males and females, respectively. The equation for regression of body weight on age was estimated as $\hat{Y}=0.1347X^2+18.738X-40.134$ ($R^2=0.9418$). Using this regression equation, the duration required to attain a KNC market-weight of 2 kg was estimated as 71.8 days. All the correlation coefficients between early chick weight and market-weight were significantly positive. Although the correlation coefficients among the chick weights in each growth period decreased with increase in age interval, early growth weight had a significant effect on late growth performance. The correlation coefficient between market-weight at 70-days and chick weight at 1-day was estimated to be a low as 0.10-0.13. In the estimations of market-weight correlation coefficients, correlation coefficient and coefficient of determination were high in the female and male chicks after 28-days and 42-days of age, respectively. The results of the analysis of correlation and regression between early chick weight and market-weight of KNCs showed that market-weight could be predicted based on the weights of 28-day-old females and 42-day-old males.