• Journal of Korean Tunnelling and Underground Space Association
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• v.22 no.4
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• pp.337-346
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• 2020

Traffic jam and congestion in urban areas has caused the need to improve the utility of underground space. In response, research on underground structures is increasingly being conducted. Notably, a double-deck tunnel is one of the most widely used of all those underground structures. This double-deck tunnel is separated by the middle slab into the upper and lower roadways. Both vehicle load and earthquake load cause the middle slab to exhibit dynamic behavior. Earthquake-related response characteristics, in particular, are highly complex and difficult to interpret in a theoretical context, and thus experimental research is required. The aim of the present study is to assess the stability of a double-deck tunnel's middle slab of the Collapse Prevention Level and Seismic Category 1 with regard to the presence of vibration-damping Rubber Bearings. In vibration table tests, the ratio of similitude was set to 1/4. Linings and vibrating platforms were fixed during scaled model tests to represent the integrated behavior of the ground and the applied models. In doing so, it was possible to minimize relative behavior. The standard TBM cross-section for the virtual double-deck tunnel was selected as a test subject. The level of ground motion exerted on the bedrock was set to 0.154 g (artificial seismic wave, Collapse Prevention Level and Seismic Category 1). A seismic wave with the maximum acceleration of 0.154 g was applied to the vibration table input (bedrock) to analyze resultant amplification in the models. As a result, the seismic stability of the middle slab was evaluated and analyzed with respect to the presence of vibration-damping rubber bearings. It was confirmed that the presence of vibration-damping rubber bearings improved its earthquake acceleration damping performance by up to 40%.

• Toxicological Research
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• v.20 no.2
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• pp.101-108
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• 2004

The different social reflections about the benefits and the potential risks of genetically modified (GM) crops have evolved with .different reactions in different countries. Many countries including Korea are working toward setting down new guidelines. Korea requires companies to label all food that contains more than 3% GM ingredients. One of the rapid and convenient detection methods of GM ingredients is amplification of the introduced DNAs by polymerase chain reaction (PCR). Many PCR kits for this purpose are commercially available. The objective of this study was to evaluate performance of commercialized GM crop detection kits. The results showed that 6 out of 15 kits tested did not meet the requirements even purposed by the manufacturers themselves in terms of stability, reproducibility, and detection limits, suggesting a potential quality control problem in their design stage or production line. The evaluation also suggests that, although the duplex and triplex detection kits allowed unambiguous detection in a single PCR reaction, the monoplex detection kits were the most sensitive to the detection of GM ingredients. The detection limits also differ between soybean and corn. Results from this study will be useful in the development of sound qualitative tracking systems of GM ingredients for monitoring throughout the cultivation of GM crops, their trans-boundary movement, and food production using GM grains as well as for complying with government guidelines associated with GM crops.

• The Transactions of the Korean Institute of Power Electronics
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• v.6 no.1
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• pp.98-106
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• 2001

This paper presents external load disturbance compensation that used to deadbeat load torque observer and regulation of the compensation gain by parameter estimator. As a result, the response of PMSM follows that of the nominal plant. The load torque compensation method is compose of a dead beat observer that is well-known method. However it has disadvantage such as a noise amplification effect. To reduce of the effect, the post-filter, which is implemented by MA process, is proposed. The parameter compensator with RLSM(recursive least square method) parameter estimator is suggested to increase the performance of the load torque observer and main controller. Although RLSM estimator is one of the most effective methods for online parameter identification, it is difficult to obtain unbiased result in this application. It is caused by disturbed dynamic model with external torque. The proposed RLSM estimator is combined with a high performance torque observer to resolve the problems. As a result, the proposed control system becomes a robust and precise system against the load torque and the parameter variation. A stability and usefulness, through the verified computer simulation and experiment, are shown in this paper.

• Journal of Astronomy and Space Sciences
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• v.19 no.4
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• pp.273-282
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• 2002

We describe some performance of the detector electronics system for the FIMS (Far-ultraviolet Imaging Spectrograph) mission. The FIMS mission to map the far ultraviolet sky uses MCP (micro-channel plate) detectors with a crossed delay line anode to record photon arrival events. FIMS has two MCP detectors, each with a ~25mm$\times$25mm active area. The unconventional anode design allows for the use of a single set of position encoding electronics for both detector fields. The centroid position of the charge cloud, generated by the photon-stimulated MCP, is determined by measuring the arrival times at both ends of the anode following amplification and external delay. The temporal response of the detector electronics system determines the readout's positional resolution for the charge centroid. High temporal resolution (<$35{\times}75$ps FWHM) and low power consumption (< 6W) were achieved for the FIMS detector electronics system.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.2
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• pp.227-233
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• 2014

This study investigates the vibration characteristics of a wire-bonding piezoelectric transducer and ultrasonic horn for high-speed and precise welding. A ring-type piezoelectric stack actuator is excited at 136 kHz to vibrate a conical-type horn and capillary system. The nodal lines and amplification ratio of the ultrasonic horn are obtained using a theoretical analysis and FEM simulation. The vibration modes and frequencies close to the driving frequency are identified to evaluate the bonding performance of the current wire-bonder system. The FEM and experimental results show that the current wire-bonder system uses the bending mode of 136 kHz as the principal motion for bonding and that the transverse vibration of the capillary causes the bonding failure. Because the major longitudinal mode exists at 119 kHz, it is recommended that the design of the current wire-bonding system be modified to use the major longitudinal mode at the excitation frequency and to minimize the transverse vibration of capillary in order to improve the bonding performance.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.8
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• pp.3792-3799
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• 2012

The dedusting characteristics of pulse air jet type dedusting system which is widely applied in the industries were identified by utilizing the computational fluid dynamics (CFD) and the dedusting performance in modified shape of dedusting unit was compared in this study. The review on the dedusting air volume, air stream distribution and inflow velocity distribution on each shape of dedusting part showed that the case of installing the nozzle on the blow tube (Case-3) and the case of installing the double intaking tube to the venturi (Case-4 and Case-5) were more excellent than the structure (Case-1) which is widely applied in the field in its amplification effect on the air volume and extension of stream width. The specification of venturi was decided to apply the selected Case-5 for the option of the commercial back filter. It is considered that the dedusting air volume will be maintained in maximum in the case of 50 mm and 90 mm for the diameter of internal and external intaking pipe respectively.

• Journal of the Earthquake Engineering Society of Korea
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• v.7 no.4
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• pp.15-22
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• 2003

Seismic design codes developed taking into account the strong earthquakes may result in unnecessary economical loss in the low seismic area, and the importance of the performance based design considering the soil-structure interaction is recognized for the reasonable seismic design. In this study. elastic and inelastic seismic response analyses of a single degree of freedom system on the soft soil layer were performed considering the nonlinearity of the soil for the 1 weak earthquakes scaled to the nominal peak accelerations of 0.07g and 0.11g. The seismic response analyses were performed in one step applying the earthquake motions to the bedrock, utilizing a pseudo 3-D dynamic analysis software of the soil-structure system. The study results indicated that seismic response spectra of a system assuming the rigid base or the linear soil layer does not represent the true behavior of a structure-soil system, and it is necessary to take into account the nonlinear soil-structure interaction effects and to perform the performance based seismic design for the various soil layers, having different characteristics, rather than to follow the routine design procedures specified in the design codes for the reasonable seismic design. The nonlinearity of the soft soil excited with the weak seismic motions also affected significantly on the elastic and inelastic seismic response spectra of a system due to the nonlinear soil amplification of the earthquake motions, and it was pronounced especially for the elastic response spectra.

• Earthquakes and Structures
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• v.23 no.4
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• pp.373-384
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• 2022

To improve the seismic performance of suspended ceiling structures, various vibration-damping devices have been developed. However, the devices made of metals have a limit in that they cause large deformation and seriously damages the exterior of the suspended ceiling structure from the wall. As a results, their strengthening effect of the suspended ceiling structure was minimal. Thus, this study employed a spring and vibration-proof rubber effectively controlled vibrations without increasing horizontal seismic loads on the ceiling to enhance the seismic resistance of suspended ceiling structures. The objective of the study is to examine the dynamic properties of a seismic damping-isolation unit (SDI) with various details developed. The developed SDI was composed of a spring, embossed rubbers, and prestressed bolts, which were the main factors enhancing the damping effect. The shaking table tests were performed on eight SDI specimens produced with the number of layers of embossed rubber (n_s), presence or absence of a spring, prestressed force magnitude introduced in bolts (f_ps), and mass weight (W_m) as the main parameters. To identify the enhancement effect of the SDI, the dynamic properties of the control specimen with a conventional hanger bolt were compared to those of the SDI specimens. The SDI specimens were effective in reducing the maximum acceleration (A_{c max}), acceleration amplification factor (α_p), relative displacement (δ_R), and increasing the damping ratio (ξ) when compared to the control specimen. The A_{c max}, α_p, and δ_R of the SDI specimens with two rubbers, spring, and f_ps of 0.1f_by, where f_by is the yielding strength of the screw bolt were 57.8%, 58.0%, and 61.9% lower than those of the conventional hanger bolt specimens, respectively, resulting in the highest ξ (=0.127). In addition, the α_p of the SDI specimens was 50.8% lower than those specified in ASCE 7 and FEMA 356. Consequently, to accurately estimate the α_p of the SDI specimens, a simple model was proposed based on the functions of f_ps, stiffness constant of the spring (K), W_m, and n_s.

• Food Science and Preservation
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• v.30 no.2
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• pp.262-271
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• 2023

The objective of this study is to compare and assess the effectiveness of real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), and the selective agar plate method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, the detection performance of the three methods (RT-PCR [SureTect^TM kit and PowerChek^TM kit], LAMP [3M MDS], selective agar) were similar at 0-10, 10-50, 50-100, and 100- CFU/mL of Salmonella spp. and L. monocytogenes. We found that with RT-PCR, the Ct value of salad was significantly higher (p<0.05) than other RTE foods, indicating that fiber plays a critical role as an obstacle to the rapid detection of Salmonella spp. However, the Ct value displayed a mixed pattern according to the inoculation level of L. monocytogenes. The use of rapid detection kits and machines mostly depends on the user's choice, with accuracy, ease of use, and economy being the primary considerations. As an RT-PCR kit, SureTect^TM and PowerChek^TM showed high accuracy in detecting Salmonella spp. and L. monocytogenes in RTE foods, showing that they can replace the existing RT-PCR kits available. Additionally, LAMP also showed excellent detection performance, suggesting that it has the potential to be used as a food safety management tool.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.