• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.1-8
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• 2016

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, $RIDA^{(R)}$ Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

• 한국전자파학회논문지
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• 제14권1호
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• pp.68-74
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• 2003

본 논문에서는 Ka-band 위성중계기에 사용되어지는 수신하향변환기(Receiver-Downconverter) 모듈의 설계 및 제작 그리고 시험 결과에 대하여 기술하였다. 개발된 수신하향변환기는 30.6 GHz∼31 GHz대역의 신호를 입력으로 받아들여 20.8 GHz∼21.2 GHz 대역의 신호를 출력하는 일종의 Downconverter 이지만, 모듈의 입력단에 저잡음 증폭부가 포함된 수신기의 역할도 하고 있다 개발된 모듈은 제작과정 및 시험과정에 있어 위성중계기 부품 제작 규정을 준수하였으며, 그 개발된 성능은 현재까지 알려진 국내외 유사 부품중의 최고의 성능을 보여주고 있다. 측정된 모듈의 성능은 상온에서 1.9 dB의 잡음지수, 55 dB 이득, 58 dBc의 C/I3 값을 보여주고 있다. 제작된 모듈의 크기는 93 mm${\times}$84 mm${\times}$26 mm이며 무게는 240 g으로서 국내외 유사 제품에 비해 소형이다.

• 한국해양공학회지
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• 제24권6호
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• pp.51-60
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• 2010

In this study, the performance evaluation of a conventional wave resonator at the entrance of a port or a pier against secondary undulation has been performed using 2D hydrodynamic modeling within port. A wave resonator has been designed for the attenuation of the secondary undulation induced by the long-periodic waves. The controlled performance of the wave resonator has been numerically investigated for CGWAVE MODULE of finite-element model of SMS (Surface water Modeling System) based on the elliptic mild-slope wave equation. SMS was verified though the comparisons with analytical solution performed by Ippen and Goda (1963). Also, It was confirmed that a wave resonator of a rectangular model harbor is effective enough to control the secondary undulation when it compares variation of water level with the case of no resonance system. From the above results, amplification phenomenon induced by long-period waves transferred from 1900 sec to 2100 sec when it applied a wave resonator in Busan Gamcheon Port which is a deep-sea. And it was confirmed that a wave resonator of Pohang New Port attenuates largely long-period waves which are within the range of 300 sec induced by long-period motion of the moored ship.

• 한국지반공학회논문집
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• 제33권12호
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• pp.45-58
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• 2017

최근 들어 시설물별 내진설계기준이 성능기반 내진설계로 전환됨에 따라, 신뢰성있는 비선형 응답이력해석(Response-history analysis, RHA)에 대한 요구가 높아지고 있다. 그러나, 부지응답해석 분야에 있어서는 1970년대 이후 등가선형 해석이 표준절차로 자리잡고 있음에 따라, 이를 대체하기 위해서는 비선형 응답이력해석의 신뢰성이 확보되어야 한다. 본 논문에서는 1989년 미국 Loma Prieta 지진기록을 바탕으로 다층지반에 대해서 비선형 RHA를 이용한 부지응답해석 결과의 신뢰성을 검증하였다. 이를 위하여, 비선형 RHA를 위한 비선형 지반모델의 선정방법과 3차원 해석시 요구되는 기반암 경계조건의 영향을 평가 하였다. 평가 결과, 제한된 조건하에서 가장 정확한 비선형 지반모델과 경계조건을 적용 시 비선형 RHA의 결과는 등가선형 해석결과와 유의미한 차이는 발생하지 않음을 알 수 있었다. 또한, 3차원 해석을 시행하는 경우, 전체 모델의 회전운동을 제어하기 위하여 최 하단부 흡수 경계조건을 적용해야 함을 알 수 있었다.

• Structural Engineering and Mechanics
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• 제62권2호
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• pp.197-208
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• 2017

Among various retrofitting strategies, use of semi-active control for retrofitting a building structure has gained momentum in recent years. One of the techniques for such retrofitting is to connect a weaker building to an adjacent stronger building by semi-active devices, so that performances of a weaker building are significantly improved for seismic forces. In this paper, a ten storey weaker building is connected to an adjacent stronger building using magneto-rheological (MR) dampers, for primarily improving the performance of the weaker building in terms of displacement, drift and base shear. For this, a fuzzy logic controller is specifically developed by fuzzyfying the responses of the coupled system. The performance of the control strategy is compared with the passive-on and passive-off controls. Pounding Mitigation between the two buildings is also investigated using all three control strategies. The results show that there exists a fundamental frequency ratio between the two buildings for which maximum control of the weaker building response takes place with no penalty on the stronger building. There exists also a fundamental frequency ratio where control of the weaker building response is achieved at the expense of the amplification of the stronger building. However, coupling strategy always improves the possibility of pounding mitigation.

• 정보처리학회논문지A
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• 제12A권6호
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• pp.531-538
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• 2005

고성능 VLSI 아날로그 정보처리시스템(AIPS)에서 고 이득 Op-Amp는 기본적 정보처리소자이다. 증폭기는 시스템 내 피드백루프에 사용시 안정도와 정확도를 얻기 위하여 고 이득이 요구된다. 1단의 증폭으로 이득이 충분하지 않을 경우 이득 부스팅 또는 추가적인 이득단이 필요하다. 본 논문에서 부 저항소자를 사용할 경우 이득이 개선되며 1단으로 고 이득을 손쉽게 얻을 수 있음을 보였다. 기존의 방법에 비교하여 본 연구에 제안된 방법은 전 출력 스윙, 적은 회로면적과 전력소비, 그리고 여러 구조의 증폭기에 적용가능 하다는 잇점을 지니고 있다. 부 저항소자는 Op-Amp에 사용될 경우 (+)와 (-) 차동출력 사이에 설치되어 증폭기 출력저항을 상쇄한다. 부 저항소자를 교차 연결된 CMOS 인버터의 형태로 구현할 경우 간단한 구조로서 40 dB 보다 더 큰 이득개선을 손쉽게 얻을 수 있음을 HSPICE 시뮬레이션을 통하여 확인하였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권10호
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• pp.4002-4014
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• 2015

ITU-R M.1842-1, as a well-known specification dedicated to maritime mobile applications, has standardized wireless transmission protocols according to the particular characteristics of a maritime communications scenario. A time division multiple access (TDMA) frame structure, along with modulation schemes to achieve a high data rate, has been described clearly in ITU-R M.1842-1. However, several specification items are still under "to be decided" status, which brings ambiguity to research works. In addition, the current version of ITU-R M.1842-1 is focused mainly on maritime transmissions in open-sea areas, where the cyclic prefix (CP) is set to zero and only 16-QAM is used in the multi-carrier (MC) system. System performance might be dramatically degraded in coastline areas due to the inter-symbol interference (ISI) caused by selective fading. This is because there is a higher probability that the signal will be reflected by obstacles in coastline areas. In this paper, we introduce the transmission resource block (TRB) dedicated to ITU-R M.1842-1 for a ship ad-hoc network (SANET), where the pilot pattern of TRB is based on the terrestrial trunked radio (TETRA). After that, we evaluated SANET performance under the maritime channel model in a coastline area. In order to avoid noise amplification and to overcome the ISI caused by selective fading, several strategies are suggested and compared in the channel estimation and equalization procedures, where the link-level simulation results finally validate our proposals.

• 한국동물위생학회지
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• 제44권3호
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• pp.163-168
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• 2021

The rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in isolating infected patients and preventing further viral transmission. In this study, we evaluated the clinical diagnostic performances of a commercial real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay (Isopollo^® COVID-2 assay, M-monitor, Daegu, Korea) using eighty COVID-19 suspected clinical samples and compared these with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (Allplex^TM 2019-nCoV rRT-QPCR Assay, SeeGene, Seoul, Korea). The results of the RRT-LAMP assay targeting the N or RdRp gene of SARS-CoV-2 showed perfect agreement with the RT-qPCR assay results in terms of detection. Furthermore, the RRT-LAMP assay was completed in just within a 20-min reaction time, which is significantly faster than about the 2 h currently required for the RT-qPCR assay, thus enabling prompt decision making regarding the isolation of infected patients. The RRT-LAMP assay will be a valuable tool for rapid, sensitive, and specific detection of SARS-CoV-2 in human or unexpected animal clinical cases.

• 전기전자학회논문지
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• 제22권4호
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• pp.953-958
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• 2018

본 연구에서는 산업현장, 지하시설물의 밀폐공간에서 유해가스 및 생체신호를 동시에 검출이 가능한 휴대형 검출시스템을 개발하였다. 개발된 시스템은 가스검출용 센서모듈, 패치형 1채널 소형 ECG 센서, 3축가속도 검출센서용 모듈 및 통계분석용 시스템이다. 시스템모듈의 성능을 검증하기 위해 디지털해상도, 생체신호증폭도, 출력전압 및 초소형모듈의 크기로 평가하였다. 개발된 시스템의 성능결과 디지털해상도는 300(rps), 신호증폭이득은 500dB이상 성능을 가졌고, 심전도 모듈은 $50mm{\times}10mm{\times}10mm$로 제작되어 패치형으로 활용도를 높일 수 있다. 본 연구의 휴대용 가스검출기 및 패치용 심전도, 가속도검출기는 산업현장작업자의 실시간 감시용 IoT 기반 관리시스템으로 활용한다면 가치가 높을 것으로 생각된다.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1716-1721
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• 2021

Chikungunya fever is an arboviral disease caused by the Chikungunya virus (CHIKV). The disease has similar clinical manifestations with other acute febrile illnesses which complicates differential diagnosis in low-resource settings. We aimed to develop a rapid test for CHIKV detection based on the nucleic acid lateral flow immunoassay technology. The system consists of a primer set that recognizes the E1 region of the CHIKV genome and test strips in an enclosed cassette which are used to detect amplicons labeled with FITC/biotin. Amplification of the viral genome was done using open-source PCR, a low-cost open-source thermal cycler. Assay performance was evaluated using a panel of RNA isolated from patients' blood with confirmed CHIKV (n = 8) and dengue virus (n = 20) infection. The open-source PCR-NALFIA platform had a limit of detection of 10 RNA copies/ml. The assay had a sensitivity and specificity of 100% (95% CI: 67.56% - 100%) and 100% (95% CI: 83.89% - 100%), respectively, compared to reference standards of any positive virus culture on C6/36 cell lines and/or qRT-PCR. Further evaluation of its performance using a larger sample size may provide important data to extend its usefulness, especially its utilization in the peripheral healthcare facilities with scarce resources and outbreak situations.