Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison of clinical diagnostic performance between commercial RRT-LAMP and RT-qPCR assays for SARS-CoV-2 detection

  • Kim, Hye-Ryung (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Jonghyun (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Han, Hyung-Soo (Department of Physiology, School of Medicine, Kyungpook National University) ;
  • Kim, Yu-Kyung (Department of Clinical Pathology, School of Medicine, Kyungpook National University) ;
  • Jeon, Hyo-Sung (M Monitor Incorporation) ;
  • Park, Seung-Chun (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University) ;
  • Park, Choi-Kyu (College of Veterinary Medicine & Animal Disease Intervention Center, Kyungpook National University)
  • Received : 2021.09.14
  • Accepted : 2021.09.29
  • Published : 2021.09.30
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Abstract

The rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in isolating infected patients and preventing further viral transmission. In this study, we evaluated the clinical diagnostic performances of a commercial real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay (Isopollo® COVID-2 assay, M-monitor, Daegu, Korea) using eighty COVID-19 suspected clinical samples and compared these with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (AllplexTM 2019-nCoV rRT-QPCR Assay, SeeGene, Seoul, Korea). The results of the RRT-LAMP assay targeting the N or RdRp gene of SARS-CoV-2 showed perfect agreement with the RT-qPCR assay results in terms of detection. Furthermore, the RRT-LAMP assay was completed in just within a 20-min reaction time, which is significantly faster than about the 2 h currently required for the RT-qPCR assay, thus enabling prompt decision making regarding the isolation of infected patients. The RRT-LAMP assay will be a valuable tool for rapid, sensitive, and specific detection of SARS-CoV-2 in human or unexpected animal clinical cases.

Keywords

Acknowledgement

This work was supported by the Commercializations Promotion Agency for R&D Outcomes (COMPA) grant funded by the Korean Government (Ministry of Science and ICT) (2021, R&D project No. 1711139487).

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