• Steel and Composite Structures
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• v.21 no.5
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• pp.1069-1084
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• 2016

This paper introduces a new and effective design amplification factor-based approach for reliable optimum design of trusses. This paper may be categorized as in the family of decoupled methods that aiming for a reliable optimum design based on a Design Amplification Factor (DAF). To reduce the computational expenses of reliability analysis, an improved version of Response Surface Method (RSM) was used. Having applied this approach to two planar and one spatial truss problems, it exhibited a satisfactory performance.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.693-697
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• 2015

Our aims were to evaluate the clinical performance of human telomerase RNA gene component (hTERC gene) amplification assay with high-risk human papillomavirus (HR-HPV) DNA test of Hybrid Capture 2 DNA test (HC2), for the detection of high grade cervical precancerous lesions and cancer (CIN 2+). In addition, the association shown between hTERC gene amplification and HPV DNA test positive in women with and without cervical neoplasia was assessed. There were 92 women who underwent cytology, HR-HPV DNA test, hTERC gene amplification test, colposcopy and biopsy. We compared the clinical performance of hTERC gene test along with HR-HPV DNA test of women with colposcopy and routine screening. The samples were histology-confirmed high-grade cervical intraepithelial neoplasia (CIN 2) or worse (CIN2+) as the positive criterion. The test of hTERC gene showed the hTERC gene amplification positivity increased with the severity of histological abnormality and cytological abnormality. The test of hTERC gene showed higher specificity than HR-HPV DNA test for high-grade lesions (84.4% versus 50%) and also higher positive predictive value (90.4% versus 76.5%). Our results predicted that hTERC gene amplification demonstrated more specific performance for predicting the risk of progression and offer a strong potential as a tool for triage in cervical cancer screening, with the limited sensitive as HR-HPV DNA test.

• Journal of Preventive Medicine and Public Health
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• v.45 no.4
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• pp.235-243
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• 2012

Objectives: Nail has been a substitute DNA source for genotyping. To investigate the integrity and consistency of nail DNA amplification for biomarker study, nail clippings from 12 subjects were collected at monthly intervals. The possibility of longer amplification and existence of GAPDH RNA/protein, were also investigated with three nail samples. Methods: Three primer sets were designed for quantitative amplification of nuclear and mitochondrial genes and analysis of their consistency. The mean threshold cycles in amplification of the target genes were compared to test the consistency of polymerase chain reaction (PCR) performance among individual factors including age groups, sex, family, the nail source, and by the size of the amplification segments. Results: The amplification of the target genes from nail DNA showed similar integrity and consistency between the nail sources, and among the serial collections. However, nail DNA from those in their forties showed earlier threshold cycles in amplification than those in their teens or seventies. Mitochondrial DNA (mtDNA) showed better DNA integrity and consistency in amplification of all three targets than did nuclear DNA (nucDNA). Over 9 kb of mtDNA was successfully amplified, and nested quantitative PCR showed reliable copy numbers (%) between the two loci. Reverse transcription PCR for mRNA and immunoblotting for GAPDH protein successfully reflected their corresponding amounts. Regarding the existence of RNA and protein in nails, more effective extraction and detection methods need to be set up to validate the feasibility in biomarker study. Conclusions: Nail DNA might be a feasible intra-individual monitoring biomarker. Considering integrity and consistency in target amplification, mtDNA would be a better target for biomarker research than nucDNA.

• Journal of the Korean Society of Safety
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• v.27 no.1
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• pp.70-75
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• 2012

For the vibration control of earthquake-excited structures, a pivot-type displacement amplification damper system is proposed and its validity is investigated in this study. A rotational frame amplifies the stroke of the proposed damper system and it can absorb more vibrational energy compared to the conventional dampers of which strokes are not large. In order to prove the effectiveness of the system, time-history analyses are performed with a three story building modelled by a three dimensional frame and numerical results are compared with those for a conventional V-shape braced damper system. In addition, the seismic performances are investigated according to the changes of damper capacity and location.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2016.10a
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• pp.11-12
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• 2016

Buildings which was constructed before 1988 were not satisfied seismic design code. Thus, most of these buildings have to retrofit for remodeling. This study proposed the steel slit damper system with displacement-amplification device. It could maximize the effectiveness of a damper system in controlling seismic response of a building by amplifying story drift induced to damper. In this study, the proposed system was verified the effect of displacement-amplification using the analytical and experimental study. From the analysis and experiment, it was found that the propsed damping system showed a stable hysteric behavior with excellent energy dissipation capacity. Therefore the proposed system will make a good seismic performance and economical benefits.

• Fisheries and Aquatic Sciences
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• v.17 no.3
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• pp.339-344
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• 2014

PCR amplification with universal primer is a useful tool for speciation of symbionts in marine eukaryote coupled with robust separation method such as denaturing high performance chromatography (DHPLC). To overcome the biased amplification, clamping PCR is recommended to suppress the amplification of host gene. In this study, we evaluated the efficiency of rare gene detection for two kinds of clamping probes which were successfully utilized for eukaryotic symbiont analysis: C3 linked nucleotide (C3) and peptide nucleic acid (PNA). PNA was 3-4 orders of magnitude higher than that of C3 tested in clamping efficiency and rare gene detection. This represented that PNA could be a more competent clamping probe for the enhancement of PCR amplification for rare symbiont genes.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2005.03a
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• pp.421-428
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• 2005

In this paper, the performance of a toggle brace-MR damper system is evaluated for the control of the structure excited by earthquake load and the non-linearity of the toggle system is investigated. Considering that the control force of MR damper described by Bingham model is a function of velocity, velocity amplification factor by toggle brace system is calculated and the effect of toggle configuration on the amplification factor is also evaluated. Numerical results show that the control performance can be largely enhanced using toggle brace system especially for the case that the MR damper installed with conventional brace system such as Chevron and diagonal cannot provide enough control force under severe earthquake load.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2004.04a
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• pp.376-380
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• 2004

This paper is to develop an optical ruer displacement sensor for monitoring high speed spindle. Proper magnitude of optical power as well as amplification of output signal are necessary to improve sensitivity of the sensor. In this paper, to meet the need of improvement of the sensor resolution, we choose proper optical power and amplification level through speculating on optical power of a laser diode.

• Journal of Electrical Engineering and information Science
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• v.2 no.3
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• pp.95-98
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• 1997

A nonlinear optical-microwave interaction is carried out in an uniplanar CPW-to-Slotline ring resonator on the semi-insulating GaAs substrate, in which a Schottky photodetector is monolithically integrated as a coupling gap. When the capacitive reactance of the detetor is modulated, the parametric amplification effect of the mixer occurs. In this device structure, the parametric amplification gain of 20 dB without the applied bias in RF signal is obtained. This microwave optoelectronic mixer can be used in the fiber-optic communication link.

• Design & Manufacturing
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• v.11 no.1
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• pp.19-25
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• 2017

A conventional taped banknotes detecting device in the banknote counting machine have some problems caused by temperature, humidity, dust and other environmental conditions. In order to the improve the detection performance of taped banknote in the banknote counting machine, we developed taped banknotes detecting device that the ultrasonic wave sensor was replaced by mechanical method device. There are many factors for the performance of the taped banknote detecting device of a mechanical method, we considered three main factors that are the amplification ratio of the thickness of banknotes, the pressing force of banknotes and the feeding speed of banknotes. We carried out the experiments with the 27 kinds of combination of these three main factors. As the result, accurate detection was possible to the normal banknotes and taped banknotes regardless of the feeding rate in terms of the amplification rate 27 times and the pressing force 660gf.