Browse > Article
http://dx.doi.org/10.7314/APJCP.2015.16.2.693

Human Telomerase Gene and High-Risk Human Papillomavirus Infection are Related to Cervical Intraepithelial Neoplasia  

Zhao, Xu-Ye (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School)
Cui, Yongm (Department of Pathophysiology, Binzhou Medical University)
Jiang, Shu-Fang (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School)
Liu, Ke-Jun (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School)
Han, Hai-Qiong (Department of Gynecology, Shanxi Tumor Hospital)
Liu, Xiao-Su (Department of Gynecology, Shanxi Tumor Hospital)
Li, Yali (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.2, 2015 , pp. 693-697 More about this Journal
Abstract
Our aims were to evaluate the clinical performance of human telomerase RNA gene component (hTERC gene) amplification assay with high-risk human papillomavirus (HR-HPV) DNA test of Hybrid Capture 2 DNA test (HC2), for the detection of high grade cervical precancerous lesions and cancer (CIN 2+). In addition, the association shown between hTERC gene amplification and HPV DNA test positive in women with and without cervical neoplasia was assessed. There were 92 women who underwent cytology, HR-HPV DNA test, hTERC gene amplification test, colposcopy and biopsy. We compared the clinical performance of hTERC gene test along with HR-HPV DNA test of women with colposcopy and routine screening. The samples were histology-confirmed high-grade cervical intraepithelial neoplasia (CIN 2) or worse (CIN2+) as the positive criterion. The test of hTERC gene showed the hTERC gene amplification positivity increased with the severity of histological abnormality and cytological abnormality. The test of hTERC gene showed higher specificity than HR-HPV DNA test for high-grade lesions (84.4% versus 50%) and also higher positive predictive value (90.4% versus 76.5%). Our results predicted that hTERC gene amplification demonstrated more specific performance for predicting the risk of progression and offer a strong potential as a tool for triage in cervical cancer screening, with the limited sensitive as HR-HPV DNA test.
Keywords
Cervical intraepithelial neoplasias; cervical cancer; human telomerase RNA gene component;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Anttila A, Kotaniemi-Talonen L, Leinonen M, et al (2010). Rate of cervical cancer, severe intraepithelial neoplasia, and adenocarcinoma in situ in primary HPV DNA screening with cytology triage: randomised study within organised screening programme. BMJ, 340, 1804.   DOI
2 Baussano I, Ronco G, Segnan N, et al (2010). HPV-16 infection and cervical cancer: modeling the influence of duration of infection and precancerous lesions. Epidemics, 2, 21-8.   DOI
3 Caraway NP, Khanna A, Dawlett M, et al (2008). Gain of the 3q26 region in cervicovaginal liquid-based pap preparations is associated with squamous intraepithelial lesions and squamous cell carcinoma. Gynecol Oncol, 110, 37-42.   DOI
4 Chen Q, Wang X, Ru Y, et al (2011). Amplification of the telomerase RNA component gene in the process of human esophageal carcinogenesis. Tohoku J Exp Med, 224, 99-104.   DOI   ScienceOn
5 Chen SM, Lin W, Liu X, et al (2012). Significance of human telomerase RNA gene amplification detection for cervical cancer screening. Asian Pac J Cancer Prev, 13, 2063-8.   DOI
6 Costa C, Espinet B, Molina MA, et al (2009). Analysis of gene status in cervical dysplastic lesions and squamous cell carcinoma using tissue microarrays. Histol Histopathol, 24, 821-9.
7 Ekeowa-Anderson AL, Purdie KJ, Gibbon K, et al (2012). AKT1 loss correlates with episomal HPV16 in vulval intraepithelial neoplasia. PLoS One, 7, 38608.   DOI
8 Hopman AH, Theelen W, Hommelberg PP, et al (2006). Genomic integration of oncogenic HPV and gain of the human telomerase gene TERC at 3q26 are strongly associated events in the progression of uterine cervical dysplasia to invasive cancer. J Pathol, 210, 412-9.   DOI
9 Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.   DOI
10 Jing L, Zhong X, Huang W, et al (2014). HPV genotypes and associated cervical cytological abnormalities in women from the Pearl River Delta region of Guangdong province, China: a cross-sectional study. BMC Infect Dis, 14, 388.   DOI
11 Kirchhoff M, Rose H, Petersen BL, et al (2001). Comparative genomic hybridization reveals non-random chromosomal aberrations in early preinvasive cervical lesions. Cancer Genet Cytogenet, 129, 47-51.   DOI
12 Liu H, Liu S, Wang H, et al (2012). Genomic amplification of the human telomerase gene (hTERC) associated with human papillomavirus is related to the progression of uterine cervical dysplasia to invasive cancer. Diagn Pathol, 7, 147.   DOI
13 Moscicki AB, Schiffman M, Kjaer S, et al (2006). Chapter 5: Updating the natural history of HPV and anogenital cancer. Vaccine, 24, 42-51.
14 Oikonomou P, Mademtzis I, Messinis I, et al (2006). Quantitative determination of human telomerase reverse transcriptase messenger RNA expression in premalignant cervical lesions and correlation with human papillomavirus load. Hum Pathol, 37, 135-42.   DOI
15 Pett MR, Herdman MT, Palmer RD, et al (2006). Selection of cervical keratinocytes containing integrated HPV16 associates with episome loss and an endogenous antiviral response. Proc Natl Acad Sci U S A, 103, 3822-7.   DOI
16 Sui W, Ou M, Dai Y, et al (2009). Gain of the human telomerase RNA gene TERC at 3q26 is strongly associated with cervical intraepithelial neoplasia and carcinoma. Int J Gynecol Cancer, 19, 1303-6.   DOI
17 Szarewski A, Ambroisine L, Cadman L, et al (2008). Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears. Cancer Epidemiol Biomarkers Prev, 17, 3033-42.   DOI
18 Trope A, Sjoborg K, Eskild A, et al (2009). Performance of human papillomavirus DNA and mRNA testing strategies for women with and without cervical neoplasia. J Clin Microbiol, 47, 2458-64.   DOI
19 Van Doorslaer K, Burk RD (2012). Association between hTERT activation by HPV E6 proteins and oncogenic risk. Virology, 433, 216-9.   DOI
20 Wang YF, Wang XS, Gao SG, et al (2013). Clinical significance of combined detection of human papilloma virus infection and human telomerase RNA component gene amplification in patients with squamous cell carcinoma of the esophagus in northern China. Eur J Med Res, 18, 11.   DOI
21 Wilting SM, Snijders PJ, Verlaat W, et al (2013). Altered microRNA expression associated with chromosomal changes contributes to cervical carcinogenesis. Oncogene, 32, 106-16.   DOI
22 Zhang Y, Wang X, Ma L, et al (2009). Clinical significance of hTERC gene amplification detection by FISH in the screening of cervical lesions. J Huazhong Univ Sci Technolog Med Sci, 29, 368-71.   DOI
23 Zheng PS, Iwasaka T, Zhang ZM, et al (2000). Telomerase activity in Papanicolaou smear-negative exfoliated cervical cells and its association with lesions and oncogenic human papillomaviruses. Gynecol Oncol, 77, 394-8.   DOI