• Parasites, Hosts and Diseases
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• v.54 no.1
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• pp.1-8
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• 2016

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round touchdown PCR-RFLP assay was developed. The assay was validated with an internal amplification control included in reactions. Performance of the assay was assessed with DNA samples of various purities, 91 control fecal samples with various parasite load, and 472 samples of unknown results. Two cysts per reaction were enough for PCR detection by the assay with exhibited specificity (Sp) and sensitivity (Se) of 100% and 93%, respectively. Taking a published small subunit rRNA reference PCR test results (6%; 29/472) as a nominated gold standard, G. lamblia was identified in 5.9% (28/472), 5.2%, (25/472), and 3.6% (17/472) by PCR assay, $RIDA^{(R)}$ Quick Giardia antigen detection test (R-Biopharm, Darmstadt, Germany), and iodine-stained smear microscopy, respectively. The percent agreements (kappa values) of 99.7% (0.745), 98.9% (0.900), and 97.7% (0.981) were exhibited between the assay results and that of the reference PCR, immunoassay, and microscopy, respectively. Restriction digestion of the 28 Giardia-positive samples revealed genotype A pattern in 12 and genotype B profile in 16 samples. The PCR assay with the described format and exhibited performance has a great potential to be adopted in basic clinical laboratories as a detection tool for G. lamblia especially in asymptomatic infections. This potential is increased more in particular situations where identification of the parasite genotype represents a major requirement as in epidemiological studies and infection outbreaks.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.1
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• pp.68-74
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• 2003

This paper describes the design and the test results of the receiver-downconverter module for a Ka-band Satellite Payload. The developed module is not only a downconverter that convert the signal of 30.6 GHz∼31.0 GHz to the signal of 20.8 GHz∼21.2 GHz but also is a receiver that has the function of low noise amplification in the front stage. It has been fabricated and tested by the qualified satellite component manufacturing process and it shows the best performance of the receiver-downconverter modules operating at Ka-band frequency up to date. The module has the performance of 1.9 dB-NF, 55 dB-Gain, and 58 dBc-C/I3 fur the two tone signals of -59 dBm input power respectively at ambient temperature. It is a small and light module with the size of 93 mm${\times}$84 mm${\times}$26 mm and the weight of 240 g.

• Journal of Ocean Engineering and Technology
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• v.24 no.6
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• pp.51-60
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• 2010

In this study, the performance evaluation of a conventional wave resonator at the entrance of a port or a pier against secondary undulation has been performed using 2D hydrodynamic modeling within port. A wave resonator has been designed for the attenuation of the secondary undulation induced by the long-periodic waves. The controlled performance of the wave resonator has been numerically investigated for CGWAVE MODULE of finite-element model of SMS (Surface water Modeling System) based on the elliptic mild-slope wave equation. SMS was verified though the comparisons with analytical solution performed by Ippen and Goda (1963). Also, It was confirmed that a wave resonator of a rectangular model harbor is effective enough to control the secondary undulation when it compares variation of water level with the case of no resonance system. From the above results, amplification phenomenon induced by long-period waves transferred from 1900 sec to 2100 sec when it applied a wave resonator in Busan Gamcheon Port which is a deep-sea. And it was confirmed that a wave resonator of Pohang New Port attenuates largely long-period waves which are within the range of 300 sec induced by long-period motion of the moored ship.

• Journal of the Korean Geotechnical Society
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• v.33 no.12
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• pp.45-58
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• 2017

As many seismic codes for various facilities are changed into a performance based design code, demand for a reliable nonlinear response-history analysis (RHA) arises. However, the equivalent linear analysis has been used as a standard approach since 1970 in the field of site response analysis. So, the reliability of nonlinear RHA should be provided to be adopted in replace of equivalent linear analysis. In this paper, the reliability of nonlinear RHA is reviewed for a layered soil layer using Loma Prieta earthquake records in 1989. For this purpose, the appropriate way for selecting nonlinear soil models and the effect of base boundary condition for 3D analysis are evaluated. As a result, there is no significant differences between equivalent linear and nonlinear RHA. In case of 3D analysis, absorbing boundary condition should be applied at base to prevent rocking motion of the whole model.

• Structural Engineering and Mechanics
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• v.62 no.2
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• pp.197-208
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• 2017

Among various retrofitting strategies, use of semi-active control for retrofitting a building structure has gained momentum in recent years. One of the techniques for such retrofitting is to connect a weaker building to an adjacent stronger building by semi-active devices, so that performances of a weaker building are significantly improved for seismic forces. In this paper, a ten storey weaker building is connected to an adjacent stronger building using magneto-rheological (MR) dampers, for primarily improving the performance of the weaker building in terms of displacement, drift and base shear. For this, a fuzzy logic controller is specifically developed by fuzzyfying the responses of the coupled system. The performance of the control strategy is compared with the passive-on and passive-off controls. Pounding Mitigation between the two buildings is also investigated using all three control strategies. The results show that there exists a fundamental frequency ratio between the two buildings for which maximum control of the weaker building response takes place with no penalty on the stronger building. There exists also a fundamental frequency ratio where control of the weaker building response is achieved at the expense of the amplification of the stronger building. However, coupling strategy always improves the possibility of pounding mitigation.

• The KIPS Transactions:PartA
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• v.12A no.6 s.96
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• pp.531-538
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• 2005

In the high performance Analog Information Processing Systems(AIPS), gain boosting or additional gain stage is required when the gain is not sufficient with one stage amplification. This work shows that high gain is neatly obtained by enhancing the gain using the negative resistance element. Compared to the conventional techniques, the proposed scheme enjoys full output swing, small circuit area and power consumption, and the applications to various configurations of amplifiers. The negative resistance element is placed between the differential output nodes when used in the Op-Amp. The HSPICE simulation indicates that enhancement of more than 40 dB is readily obtained in this simple configuration when the negative resistance element is implemented in the form of cross-coupled CMOS inverters.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.10
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• pp.4002-4014
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• 2015

ITU-R M.1842-1, as a well-known specification dedicated to maritime mobile applications, has standardized wireless transmission protocols according to the particular characteristics of a maritime communications scenario. A time division multiple access (TDMA) frame structure, along with modulation schemes to achieve a high data rate, has been described clearly in ITU-R M.1842-1. However, several specification items are still under "to be decided" status, which brings ambiguity to research works. In addition, the current version of ITU-R M.1842-1 is focused mainly on maritime transmissions in open-sea areas, where the cyclic prefix (CP) is set to zero and only 16-QAM is used in the multi-carrier (MC) system. System performance might be dramatically degraded in coastline areas due to the inter-symbol interference (ISI) caused by selective fading. This is because there is a higher probability that the signal will be reflected by obstacles in coastline areas. In this paper, we introduce the transmission resource block (TRB) dedicated to ITU-R M.1842-1 for a ship ad-hoc network (SANET), where the pilot pattern of TRB is based on the terrestrial trunked radio (TETRA). After that, we evaluated SANET performance under the maritime channel model in a coastline area. In order to avoid noise amplification and to overcome the ISI caused by selective fading, several strategies are suggested and compared in the channel estimation and equalization procedures, where the link-level simulation results finally validate our proposals.

• Korean Journal of Veterinary Service
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• v.44 no.3
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• pp.163-168
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• 2021

The rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays a key role in isolating infected patients and preventing further viral transmission. In this study, we evaluated the clinical diagnostic performances of a commercial real-time reverse transcription loop-mediated isothermal amplification (RRT-LAMP) assay (Isopollo^® COVID-2 assay, M-monitor, Daegu, Korea) using eighty COVID-19 suspected clinical samples and compared these with the results of a commercial real-time reverse transcription polymerase chain reaction (RT-qPCR) assay (Allplex^TM 2019-nCoV rRT-QPCR Assay, SeeGene, Seoul, Korea). The results of the RRT-LAMP assay targeting the N or RdRp gene of SARS-CoV-2 showed perfect agreement with the RT-qPCR assay results in terms of detection. Furthermore, the RRT-LAMP assay was completed in just within a 20-min reaction time, which is significantly faster than about the 2 h currently required for the RT-qPCR assay, thus enabling prompt decision making regarding the isolation of infected patients. The RRT-LAMP assay will be a valuable tool for rapid, sensitive, and specific detection of SARS-CoV-2 in human or unexpected animal clinical cases.

• Journal of IKEEE
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• v.22 no.4
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• pp.953-958
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• 2018

In this study, a portable detection system was developed that can detect harmful gas and signals simultaneously in an enclosed space of industrial sites and underground facilities. The developed system is a sensor module for gas detection, a patch type 1 channel small ECG sensor, a module for three-axial acceleration detection sensor, and a system for statistics. In order to verify the performance of the system modules, the digital resolution, signal frequency, output voltage, and ultra-small modules were evaluated. As a result of the performance of the developed system, the digital resolution was 300 (rps) and the signal amplification gain was 500 dB or more, and the ECG module was manufactured with $50mm{\times}10mm{\times}10mm$ to increase patch utilization. It is believed that the product of this research will be valuable if it is used as an IoT-based management system for real-time monitoring of industrial workers.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1716-1721
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• 2021

Chikungunya fever is an arboviral disease caused by the Chikungunya virus (CHIKV). The disease has similar clinical manifestations with other acute febrile illnesses which complicates differential diagnosis in low-resource settings. We aimed to develop a rapid test for CHIKV detection based on the nucleic acid lateral flow immunoassay technology. The system consists of a primer set that recognizes the E1 region of the CHIKV genome and test strips in an enclosed cassette which are used to detect amplicons labeled with FITC/biotin. Amplification of the viral genome was done using open-source PCR, a low-cost open-source thermal cycler. Assay performance was evaluated using a panel of RNA isolated from patients' blood with confirmed CHIKV (n = 8) and dengue virus (n = 20) infection. The open-source PCR-NALFIA platform had a limit of detection of 10 RNA copies/ml. The assay had a sensitivity and specificity of 100% (95% CI: 67.56% - 100%) and 100% (95% CI: 83.89% - 100%), respectively, compared to reference standards of any positive virus culture on C6/36 cell lines and/or qRT-PCR. Further evaluation of its performance using a larger sample size may provide important data to extend its usefulness, especially its utilization in the peripheral healthcare facilities with scarce resources and outbreak situations.