• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.2
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• pp.125-130
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• 2015

The objective of this study was to investigate the feasibility of using near infrared spectroscopy (NIRS) to discriminate between grass spices. A combination of NIRS and chemometrics was used to discriminate between Italian ryegrass, perennial ryegrass, and tall fescue seeds. A total of 240 samples were used to develop the best discriminant equation, whereby three spectra range (visible, NIR, and full range) were applied within a 680 nm to 2500 nm wavelength. The calibration equation for the discriminant analysis was developed using partial least square (PLS) regression and discrimination equation (DE) analysis. A PLS discriminant analysis model for the three spectra range that was developed with the mathematic pretreatment "1,8,8,1" successfully discriminated between Italian ryegrass, perennial ryegrass, and tall fescue. An external validation indicated that all of the samples were discriminated correctly. The discriminant accuracy was shown as 68%, 78%, and 73% for Italian ryegrass, perennial ryegrass, and tall fescue, respectively, with the NIR full-range spectra. The results demonstrate the usefulness of the NIRS-chemometrics combination as a rapid method for the discrimination of grass species by seed.

• Horticultural Science & Technology
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• v.33 no.3
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• pp.340-348
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• 2015

Mowing turfgrasses, especially fast growing species like perennial ryegrass, is one of the most time and money consuming tasks of their management. Trinexapac-ethyl (TE) is a popular plant growth regulator used to reduce mowing requirements, improve stress tolerance, and enhance turf quality. This study was conducted to investigate the effect of TE rate and frequency of applications on growth response and traffic tolerance of perennial ryegrass. The experiment was a split-plot laid out in a randomized complete block (RCB) design with three replications. TE was applied to main plots at 0.00, 0.25, and $0.50kg\;a.i.\;ha^{-1}$. Application pattern included an initial application, followed by two sequential applications at 6-wk intervals. Traffic treatment was applied to subplots with a cleated roller. Results demonstrated that TE consistently reduced vertical shoot growth, clippings dry weight, with maximum growth reduction of 59% and 65%, for 0.25 and $0.50kg\;a.i.\;ha^{-1}$, respectively, occurring at 2 weeks after initial TE treatment (WAT). Traffic also dramatically reduced vertical shoot growth and clippings dry weight. Overall, quality of perennial ryegrass was enhanced by sequential TE applications, however, turf quality and surface coverage reduced greatly under traffic, regardless of TE treatment. Total chlorophyll, chlorophyll a and chlorophyll b and total carbohydrates (TC) contents were also positively influenced following sequential TE application. Our results indicated that TE reduces mowing frequency and enhances turf quality rather than influencing traffic resistance.

• Journal of Microbiology and Biotechnology
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• v.30 no.8
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• pp.1149-1155
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• 2020

Silage is one of the main feed components of ruminants around the world and can make up about 50-80% of the rations of dairy cows during the winter. The aim of this study was to evaluate the usability of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) aqueous and ethanol extracts to improve the hygienic quality of perennial ryegrass, red clover and blue alfalfa silage samples and estimate their effect on mycotoxins concentrations. Under laboratory conditions, 63 silage samples (21 perennial ryegrass, 21 blue alfalfa, 21 red clover) were fermented with inserted aqueous and ethanol extracts of oregano and thyme and two commercial inoculants with mesophilic lactic acid bacteria. After 96 days of fermentation, in silage samples were established fermentation parameters, microbiological status and mycotoxins concentrations. It was determined that the best results for achieving hygienic quality of perennial ryegrass and red clover silage samples was by insertion of aqueous and ethanol extracts of oregano and thyme. In blue alfalfa samples, the best results of silage hygienic indicators were determined by inserting aqueous and ethanol extracts of oregano. Aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZEA) and T-2 toxin concentrations in perennial ryegrass, red clover and blue alfalfa silage samples were best reduced by inserting aqueous and ethanol extracts of oregano and thyme. The present study shows that these extracts can be used to improve silage hygienic quality, reduce mycotoxins concentrations and thus ensure the wellness of cattle.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.235-240
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• 2007

Optimum tissue culture conditions for an efficient induction of embryogenic callus from mature seeds of perennial ryegrass (Lolium perenne L.) and regeneration of plants from callus tissues were investigated. MS medium containing 3 mg/L 2,4-D and 0.1 mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (58.3%) was observed when the embryogenic callus tissues were cultured on N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be helpful for molecular breeding of perennial ryegrass through Agrobacterium-mediated genetic transformation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.3
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• pp.122-127
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• 1987

This experiment was carried out to investigate the effect of shading degrees (0:full sunlight, 25, 50 and 75%) on the crop growth rate (CGR), net assimilation rate (NAR) and photosynthetic activity of grasses for obtaining the basic data on the development, management and utilization of pasture in the forest. For the test, different artificial shading houses were established and pasture species used in this study were orchardgrass, timothy, perennial ryegrass and ladino clover. The experiment was performed at Livestock Experiment Station, Suwon, in 1984-'85. The results obtained are summarized as follows. 1. The highest CGR was obtained at 0% (full sunlight) and 25% of shading, followed by 50 and 75% of shading degrees, in that order. CGR of orchardgrass and perennial ryegrass were higher than those of timothy and ladino clover. 2. NAR was significantly decreased as the shading degrees increased. The highest NAR was observed at perennial ryegrass plot. 3. The photosynthetic activity during summer season was the highest at 25% of shading, followed by 50, 0 and 75% of shading degrees. While, the photosynthetic activity during fall season was the highest at 25% of shading, followed by 50, 0 and 75% of shading degrees, in that order. Those of orchardgrass and perennial ryegrass were remarkably higher than that of timothy. 4. A significant positive correlation was obtained in the relationship between CGR and NAR of main grasses, regardless of pasture species and investigated date.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.2
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• pp.79-86
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• 1987

This study was carried out to investigate the relationships and the variations of agronomic characteristics in the shoot and root part of grasses for the basic data of the development, the management and the utilization of pasture in the forest. This study was conducted with 0% (full sunlight), 25%, 50% and 75% of shading degrees after orchardgrass, timothy, perennial ryegrass, ladino clover and orchardgrass dominated mixture were planted on Aug. 25 in 1984. The results are summarized as follows: 1. Dry matter yield in 25% of shading was increased 3-1 7% compared with 0% (full sunlight), while 50 and 75% of shading were decreased 28-35% and 44-60%, respectively. Dry matter yield of timothy, perennial ryegrass and ladino clover were decreased 10, 8 and 33%, respectively, compared with orchardgrass, but there was little difference between orchard grass and mixture. 2. Root distribution by different shading degrees was the maximum at 25%, followed by 0, 50 and 75% of shading degrees. Root distribution among different species was the maximum at orchardgrass, followed by perennial ryegrass and timothy. Especially, root distribution of timothy was very little compared with different species. And significantly positive correlation was observed in the relationship between root weight and dry matter yield (r=0.8 102**). 3. The physiological activity of root by different shading degrees was declined by more shading. 4. The length and the percentage of blade by different shading degrees was an increase tendency by more shading. The length and the percentage of blade among different species was followed by timothy, perennial ryegrass and orchardgrass, in that order. And the correlation between the percentage of blade and dry matter yield was significantly negative.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.24-30
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• 2010

We investigated occurrence of yellow patch for early spring season of 48 cultivars in 5 species of turfgrass, most popularly used in Korea golf courses. Only yellow patch disease caused by Rhizoctonia cerealis was occurred naturally from late fall season. Perennial ryegrass and creeping bentgrass cultivars showed susceptibility from yellow patch in early spring, respectively. However, among There was no significantly different between the other species. Creeping bentgrass, 'Pennlinks II' cultivar was the most susceptible. Yellow patch symptom showed reddish brown with margins in color and patch showed size from 5 cm to 50 cm in diameter. Based on our results, Yellow patch showed large differences among cultivar and each turfgrass species.

• Asian Journal of Turfgrass Science
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• v.9 no.3
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• pp.253-260
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• 1995

This study is aimed to understand the effects of temperature, light and GA soaking treatment on the seed germination of perennial ryegrass 'Dandy', creeping hentgrass 'Penlinks', tall fescue 'Arid' and Kentucky hluegrass 'Nasaw'. These experiments are carried in vitro, and their results obtained are summarized as the followings ; The optimum temperature for seed germination of perennial ryegrass 'Dandy' was found to he ranged at 20~30$^{\circ}C$, and the optimum temperature for germination of creeping bentgrass 'Penlinks', tall fescue 'Arid' and kentucky bluegrass 'Nasaw' seeds was found to be ranged at 20~25$^{\circ}C$, whereas the germination percentage under the below or above temperature of this range decreased. The germination of perennial ryegrass 'Dandy' and tall fescue 'Arid' seeds showed no effect on light, and while the germination percentage of creeping hentgrass 'Penlinks' increased more highly under the dark condition than under the light condition. The germination percentage decreased according to the higher temperature. The germination of Kentucky bluegrass 'Nasaw' seeds increased more highly under the dark condition than under the light condition at 20~25$^{\circ}C$, whereas the germination percentage under the dark condition at 15$^{\circ}C$ and 30$^{\circ}C$ decreased. In case of GA soaking treatment of seeds, all the cool season turfgrasses tested showed their own promoting effects on seed germination. The early germination of tall fescue 'Arid' was good without the soaking times. Creeping bentgrass 'Penlinks' and perennial ryegrass 'Dandy' had re-spectively the highest germination percentage at 10min and 1hr soaking treatment, and the longer the soaking time, the lesser the germination percentage. Kentucky bluegrass 'Nasaw' obtained the best results at 24has soaking treatment, and the longer the soaking time, the better the ger-mination percentage.

• Journal of Animal Science and Technology
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• v.46 no.3
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• pp.443-450
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• 2004

To examine the potentiality of grassland dominated with turf-type grasses, which are mainly used for the establishment of green spaces, forage production and its utilization as forage resources, dry matter yield and forage quality of three different turf-type mixtures were estimated throughout three consecutive experimental years. According to the experiment, the mean of dry matter(DM) yield, chemical composition, DM digestibility, crude protein dry matter(CPDM) and digestible dry matter(DDM) yield was not significantly different among species(p > 0.05) over three experimental years. Botanical composition of three dominant species(tall fescue, Kentucky bluegrass and perennial ryegrass) was 42, 35 and 28%, respectively, in three mixtures at the last cutting(6th) in 2001. The botanical composition of three dominated species such as tall fescue, Kentucky bluegrass and perennial ryegrass was maintained 84% of the total plants in all types of mixtures. Therefore, It is observed that the mixture types did not closely affect the botanical composition. But botanical composition of the other species such as redtop(8%) and creeping bentgrass(6${\sim}$7%) was low. Especially, that of red fescue was the least(1${\sim}$2%) of all mixed species. As mentioned above, any difference was not observed in DM yields, forage quality and botanical composition among mixture types. Therefore, any types of mixtures can be used as public green spaces like school ground areas or river-sides, and as forage resources additionally.

• Asian Journal of Turfgrass Science
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• v.15 no.4
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• pp.169-179
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• 2001

This study was conducted to determine the effect of root zone cooling on growth and quality of turfgrasses including Kentucky bluegrass (Poa pratensis L.‘Nuglade’), perennial ryegrass (Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb.‘Pixie’), and Japanese lawngrass (Zoysia japonica Steud.) in simulated athletic field during summer season in Korea. Mineral contents in clippings of turfgrasses grown at different soil mixtures and temperatures were also analyzed. Root zone cooling (approximately 4~6$^{\circ}C$ lower than that of untreated-control) resulted in good uniformity, little disease incidence and higher level of chlorophyll contents in cool-season turfgrasses. The effectiveness of root zone cooling in protecting disease incidence from high temperature stress was the most manifest in perennial ryegrass compared to others. Fresh clipping weight in treatment of root zone cooling was increased approximately 2 times in Kentucky bluegrass and perennial ryegrass, and 2.5 times in tall fescue compared to those of control. There was higher growth rate in a soil mixture composed of 80% peat moss ＋10% sand ＋10% soil (v/v/v) than in that of 80% pea moss ＋20% sand (v/v), Mineral contents of N, P, K, Ca, and Mg in clippings of three species of cool-season turfgrasses were significantly increased in treat-ment of root zone cooling but this was not found in Japanese lawngrass. Results showed that root zone cooling has a benefit in keeping good quality and growth of cool-season turfgrasses in sports field under supraoptimal ambient temperature during summer season.