• Research in Plant Disease
• /
• v.17 no.3
• /
• pp.280-287
• /
• 2011

This investigation was undertaken to survey toxigenic fungal contamination of various rice samples in 93 rice processing complexes (RPC) in Korea. Rice was grown in 2009 and the samples were collected in 2010. Seven types of rice samples such as unhusked, brown, blue-tinged, discolored, polished, half-crushed, and rice husks were obtained from each RPC. One-hundred and five grains of each sample were placed on PDA plates after surface disinfection. The incidence of fungal contaminants was 26.8%. Aspergillus spp. was the most dominant fungal contaminants and Fusarium spp. was the most frequently occurred in samples. The heaviest Fusarium contamination was found in unhusked grain, rice husks, and bare blue-tinged rice and followed by colored rice whereas broken rice was the least contaminated. Regional difference of fungal contamination was distinctive. Fusarium incidence in the rice samples from southern region of Korea including Jeolla and Gyeongsang Provinces was higher than those from central region including Chungcheong, Gyeonggi, and Gangwon Provinces. In contrast to Fusarium spp., Aspergillus spp. and Penicillium spp. were dominated in brown and polished rice samples and their incidences were more severe in central region than southern region. The major contaminants shown more than 1% of kernels infected were Aspergillus (5.0%), Fusarium (2.0%), Alternaria (1.4%), Dreschlera (1.3%), Penicillium spp. (1.3%), and Nigrospora spp. (1.0%). Collectotrichum, Pyricularia, Myrothecium, Epicoccum, Cladosporium, Moniliella, Gloeocercospora, Chaeto- mium, Curvularia, Phialopora, Acremonium, Gliomastix, Trichoderma, Rhizopus, Phomopsis, Paecilomyces, Genicularia, Geotrichum, Acremoniella, Rhizoctonia, Phoma, Oidiodendran, and Candida spp. were among the rest observed at low incidence. The major contaminants of rice samples were well-known as toxigenic fungal genera so toxin producibility of these fungal isolates is necessary to be examined in future. It is also needed to study Myrothecium spp. on species level as it was detected for the first time in rice.

• The Korean Journal of Mycology
• /
• v.44 no.4
• /
• pp.207-219
• /
• 2016

Aspergillus, Penicillium, and their teleomorphic genera have a worldwide distribution and large economic impacts on human life. The names of species in the genera that have been reported in Korea are listed in this study. Fourteen species of Aspergillus, 4 of Eurotium, 8 of Neosartorya, 47 of Penicillium, and 5 of Talaromyces were included in the National List of Species of Korea, Ascomycota in 2015. Based on the taxonomic system of single name nomenclature on ICN (International Code of Nomenclature for algae, fungi, and plants), Aspergillus and its teleomorphic genera such as Neosartorya, Eurotium, and Emericella were named as Aspergillus and Penicillium, and its teleomorphic genera such as Eupenicillium and Talaromyces were named as Penicillium (subgenera Aspergilloides, Furcatum, and Penicillium) and Talaromyces (subgenus Biverticillium) in this study. In total, 77 species were added and the revised list contains 55 spp. of Aspergillus, 82 of Penicillium, and 18 of Talaromyces.

• Journal of Environmental Health Sciences
• /
• v.27 no.2
• /
• pp.1-9
• /
• 2001

This study was carried out to investigate the microbial characteristics of the urban air pollution in the subway stations, streets, department stores, wholesale markets, underground shopping centers, buildings, parks, houses and apartments in the Seoul and the suburbs area. Total cell count, total mold count and the presence of opportunistic pathogens(Streptococcus pneumoniae, Klebsiella pneumoniae, Aspergillus spp., Penicillium spp.) were evaluated determine the microbial air quality. Total cell count and mold count of indoor air in the houses and apartments were 2.9$\times$10$^2$-6.3$\times$10$^2$cfu/㎥ and 60-1.8$\times$10$^2$cfu/㎥, respectively, and the department stores and wholesale markets had much lower cell count than the houses and apartments. Ground level of commercial stations were 2.6 fold higher than the general subway stations, and Apergillus spp. and Penicillium spp. which could cause the bronchus and lung diseases were detected 17 sampling site out of 45. Dust were collected from the commercial facilities and houses, and total cell and mold count of the dust were 4.3$\times$10$^3$-1.7$\times$10$^{6}$ cfu/g and 2.3$\times$10$^3$-6.5 $\times$10$^4$cfu/g, respectively. Therefore the dust might be one of the main reservoir of microorganims.

• Microbiology and Biotechnology Letters
• /
• v.10 no.3
• /
• pp.191-196
• /
• 1982

In order to prevent the losses of the rice by fungal deterioration during storage, fungal contaminants were isolated and identified from the grain samples (Milyang Nr.23) stored for seven months from December, 1978 to June, 1979 in silo, flat store and Tongari. Out of thirty cultures isolated from Korean paddy and brown rice samples, twenty seven species were identified, and there are eleven species of Aspergillus (A. caespitosus, A. condidus, A. chevalieri, A fischeri, A. fumigatus, A. flavus, A. nidulans, A. oryzae, A. ruber, A. sydowii, A. versicolor), five species of Penicillium (P. atramentosum, p. chrysogenum, P. cyaneofulvum, P. nototum, P. steckii), two species of each Alternaria (Al. faesiculata, Al. grisea) and Curvalaria (C, interseminata, C. tetromea), and one species of each Trichothecium roseum, Nigrospora sphaerica, Rhizopus nigricans, Fusarium spp., Mucor spp., Helminthosporium spp., and Gliocladiopsis spp. The major types of fungi grown on the surface of paddy during storage were A. flavus and A. candidus, while A. ruber and A. sydowii appeared in brown rice samples. And also A. candidus, A. versicolor and A. glacus groups were considered as major deteriorating microorganisms in stored brown and paddy rice in Korea.

• The Korean Journal of Mycology
• /
• v.29 no.1
• /
• pp.28-33
• /
• 2001

This study was done with 105 cases of dermatomycosis of dermatological patients who had visited the dermatology clinic in Taejon city. Of dermatophytosis, the decreasing orders of clinical location by the incidence were Tinea pedis (44.8%), Tinea cruris (30.5 %) and Tinea corporis (10.5%). The organisms identified from dermatophytoses were 6 genus: the decreasing orders of frequency were Trichophyton spp. (43.5%), Candida spp. (12.9%), Microsporium spp. (3.2%) and Epdermophyton flocosum(1.6%). The most common causative organisms was Trichophyton spp.

• Mycobiology
• /
• v.46 no.1
• /
• pp.52-63
• /
• 2018

In our previous studies, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15 have been shown to be antagonistic to Aspergillus flavus in stored rice grains. In this study, the biocontrol activities of these strains were evaluated against Aspergillus candidus, Aspergillus fumigatus, Penicillium fellutanum, and Penicillium islandicum, which are predominant in stored rice grains. In vitro and in vivo antifungal activities of the bacterial strains were evaluated against the fungi on media and rice grains, respectively. The antifungal activities of the volatiles produced by the strains against fungal development and population were also tested using I-plates. In in vitro tests, the strains produced secondary metabolites capable of reducing conidial germination, germ-tube elongation, and mycelial growth of all the tested fungi. In in vivo tests, the strains significantly inhibited the fungal growth in rice grains. Additionally, in I-plate tests, strains KU143 and AS15 produced volatiles that significantly inhibited not only mycelial growth, sporulation, and conidial germination of the fungi on media but also fungal populations on rice grains. GC-MS analysis of the volatiles by strains KU143 and AS15 identified 12 and 17 compounds, respectively. Among these, the antifungal compound, 5-methyl-2-phenyl-1H-indole, was produced by strain KU143 and the antimicrobial compounds, 2-butyl 1-octanal, dimethyl disulfide, 2-isopropyl-5-methyl-1-heptanol, and 4-trifluoroacetoxyhexadecane, were produced by strain AS15. These results suggest that the tested strains producing extracellular metabolites and/or volatiles may have a broad spectrum of antifungal activities against the grain fungi. In particular, B. megaterium KU143 and P. protegens AS15 may be potential biocontrol agents against Aspergillus and Penicillium spp. during rice grain storage.

• The Korean Journal of Mycology
• /
• v.36 no.2
• /
• pp.116-122
• /
• 2008

Fungal floras were investigated for the lysimeter soil treated with municipal sewage sludge (MSS), pig manure compost (PMC), industrial sewage sludge (ISS), leather processing sludge (LS), and alcohol fermentation processing sludge (FS). Fungal populations were higher in the FS, ISS, LS, or MSS-treated soil than in the chemical fertilizer-treated soil. Isolated fungi from the sewage sludge were identified as Penicillium spp., Gliocladium spp., Acremonium spp., Trichoderma spp., Aspergillus spp., Blastomyces spp., and Phoma spp.

• Journal of Life Science
• /
• v.8 no.5
• /
• pp.537-549
• /
• 1998

This investigation was performed to isloate and identify the total bacteria, Staphylococcus spp. and fungi from the indoor air exposed for 30 minutes on the blood agar plate at the 27 places in a hospital. Antibiotic susceptibility tests of the isolated bacteria were also studied. The mean numbers of total bacteria, Staphylococci spp. and fungi were 26, 17, and 2 in the summer and 19, 8, and 2 in the winter, respectively. Staphylococcus epidermidis was the most common isolated bacteria, and the next was Staphylococcus aureus, Aerococcus spp., Micrococcus spp., and Bacillus spp. from the indoor-air of hospital. Aspergillus spp., Cephalosporum spp., Curvularia spp., penicillium spp., and Phialophora spp. was frequently isolated from the indoor-air of hospital. The 109 strains of isolated Staphylococcus epidermidis sho-wed resistance to tetracycline(45.0%), methicillin(40.2%), erythromycin(31.2%), and kanamycin(24.8%). The 76 strains of isolated Staphylococcus aureus showed resistance to erythromycin(71.7%), methicillin(63.2%), kanamycin (44.7%), tetracycline(39.5%), and ampicillin(32.9). The 67 strains of isolated Aerococcus spp. showed resistance to erythromycin(26.9%), methicillin(25.4%), kanamycin(22%), and tetracycline(22.4%). The 48 strains of isolated Micrococus spp. showed resistance to tetracycline(27.0%), methicillin(22.9%), erythromycin(22.9%), and kanamycin(20.8%).

• Journal of Environmental Health Sciences
• /
• v.29 no.1
• /
• pp.1-7
• /
• 2003

This study was carried out to investigate the airborne microbial pollution in hospital environment. Using a mechanical air sampler, microbiological samples were taken from intensive care unit, general ward room, patients wailing room and outdoor of 20 hospitals in Seoul, Korea. The concentration of airborne bacteria and fungi ranged 97-410 cfu/㎥ and 37-77 cfu/㎥, respectively and patients waiting room had highest bacterial count. 10 genera of molds were identified and the most frequently recovered molds were Aspergillus, followed by Penicillium, Alternaria and Cladosporium. Among Staphylococcus species, S. haemolyticus and S. epidermidis were predominant and 47% of Staphylococcus species were isolated from intensive rare unit.

• Korean Journal Plant Pathology
• /
• v.14 no.5
• /
• pp.531-535
• /
• 1998

This study was conducted to test the effect of chitosan, grape fruit seed extracts(GFSE) and sodium hypochloride gas on the control of mycotoxin producing pathogens occurred kin postharvest grains. Among the treatments, sodium hypochloride gas showed the highest control effect on wheat, soybean and corn see maintained in natural conditions after postharvest and GFSE had a little control effect, but chitosan treatment had no effect. Sodium hypochloride gas exhibited the strongest control effect on the major mycotoxin producing pathogens such as Penicillium spp. Aspergillus spp. and Fusarium spp., whereas GFSE had a little control effect. Sodium hypochloride gas appeared to be effective when the grains were treated with this gas more than 24 hours.