• Korean Journal of Weed Science
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• v.9 no.2
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• pp.123-129
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• 1989

1) Optimum temperature was $15^{\circ}C$ for C. bursa-pastoris, $20^{\circ}C$ for C. album, $30^{\circ}C$ for P. oleracea, A. lividus, E. crus-galli, D. sanguinalis, and 4 showed wide range of germination temperature. 2) Emergence of C. bursa-pastoris, and C. album was best at $14.8^{\circ}C$ of soil temperature, E. crus-galli at $23^{\circ}C$, E. indica, A. lividis and P. oleracea at $27.1^{\circ}C$ and A. retroflexus and D. sanguinalis at $31.1^{\circ}C$. 3) A. retroflexus and P. oleracea started to germinate at 30% water absorption stage and A. lividus, C. album, S. viridis, and D. sanguinalis at 40% and E. indica at 70%. 4) Germination of weed species was decreased as PEG 6000 induced osmotic potential lowered. C. album, P. oleracea, D. sanguinalis, A. lividus, and Solanum nigrum were germinated at -5.0 bar osmotic potential and C. album and P. oleracea were germinated at -7.0 bar.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.68-68
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• 2020

최근 농촌진흥청과 기상청에서 발표한 연구자료에 따르면 2020년 기준으로 국내 경지면적의 10% 가량이 이미 아열대 기후대에 속하고 있을 뿐 현재 추세대로 온실가스가 배출될 경우 2100년경 우리나라의 대부분 지역은 아열대 기후대에 속하게 될 것으로 전망되고 있다. 이에 국가 기관뿐만 아니라 많은 지자체에서 기후변화에 대응하여 아열대 작물을 도입하기 위한 시도를 활발하게 추진하고 있다. 아열대 작물 중 국내에서 3번째로 많이 활용되고 있는 삼채는 기능성 물질을 다량으로 함유하고 있어 수요도가 지속적으로 증가하고 있다. 하지만, 삼채의 경우 일반적인 원예 작물과는 달리 실생 종자를 상업적으로 이용하고 있어 종자들의 발아율이 균일하지 못할 뿐만 아니라 발아율이 낮은 문제가 있는 것으로 보고되고 있다. 이에 삼채의 경우 농가에서 정식 시기를 맞추기 어려울 뿐만 아니라 대규모 생산을 위한 공정육묘 시스템의 적용이 어려운 상황에 있다. 종자 프라이밍 기술은 잠재적으로 종자 발아와 식물 생장을 빠르고 균일하게 해줄 수 있는 방법으로 알려져 있지만, 현재 삼채에서는 프라이밍 처리가 종자 발아에 미치는 영향에 대해서 검토한 사례가 없다. 따라서 본 연구는 삼채의 파종 효율을 증진시키기 위한 목적으로 몇 가지 프라이밍 처리 방법이 삼채의 발아특성에 미치는 영향을 검정하고자 하였다. 삼채 종자는 (주)아람종묘사의 종자를 구매하여 강릉원주대학교 생명과학대학에서 수행하였다. 프라이밍 처리는 Hydropriming은 증류수, Osmopriming은 CaCl2 1.0%, Halopriming은 Polyrthylene glycol 6000(PEG) 13.5%의 3가지를 사용하였으며, 각 프라이밍 처리 시간은 6시간, 12시간, 18시간, 24시간, 48시간 총 5가지 방법으로 처리되어 대조구를 포함 총 16개의 처리를 하였다. 프라이밍 처리 된 종자는 페트리디쉬 (50mm)에 30립씩 치상하였다. 프라이밍 각 처리구별 4반복으로 하였으며 24시간 암(Black), 온도 20℃의 조직배양실에서 치상 후 10일간 조사하였다. 본 실험 결과 대조구는 38% 발아율을 가졌으며, 프라이밍 처리 시간에 따른 각 처리구들은 대조구에 비해 발아율이 높아짐을 확인할 수 있었다. 그 중 Hydropriming 12시간 발아율 51%, Osmopriming 18시간 발아율 68%, Halopriming 48시간 발아율 65%로 각 프라이밍 처리구별 가장 좋은 발아율을 확인할 수 있었다. 결론적으로 삼채에서 프라이밍 처리 기술의 적용은 발아율의 향상과 발아기간의 정형화를 유도할 수 있어 농가에서의 일반 육묘는 물론이고 대규모 공정육묘를 위한 저렴하고 안전한 방법 중 하나로써 이용될 수 있을 것으로 기대되어 진다.

• The Journal of Natural Sciences
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• v.4
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• pp.103-142
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• 1991

These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

• The Korean Journal of Nuclear Medicine
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• v.33 no.6
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• pp.475-483
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• 1999

Purpose: Complete fibrovascular ingrowth within the hydroxyapatite ocular implant is necessary for peg drilling which is performed to Prevent infection and to provide motility to the ocular prosthesis. We compared planar bone scintigraphy and SPECT for the evaluation of the vascularization within hydroxyapatite ocular implants. Materials and Methods: Seventeen patients (M:F: 12:5, mean age; $50.4{\pm}17.5$ years) who had received a coralline hydroxyapatite ocular implant after enucleation surgery were enrolled. Patients underwent Tc-99m MDP planar bone and SPECT imaging by dual head gamma camera after their implant surgery (interval: $197{\pm}81$ days). Uptake on planar and SPECT images was graded visually as less than (grade 1), equal to (grade 2), and greater than (grade 3) nasal bridge activity. Quantitative ratio of implanted to non-implanted intraorbital activity was also measured. Vascularization within hydroxyapatite implants was confirmed by slit lamp examination and ocular movement. Results: All but three patients were considered to be vascularized within hydroxyapatite implants. In visual analysis of planar image and SPECT, grade 1 was noted in 9/18 (50%) and 6/18 (33%), respectively. Grade 2 pattern was 7/18 (39%) and 4/18 (22%), and grade 3 pattern was 2/18 (11%) and 8/18 (44%) respectively. When grade 2 or 3 was considered to be positive for vascularization, the sensitivity of planar and SPECT imaging were 60% (9/15) and 80% (12/15), respectively. In 3 patients with incomplete vascularization, both planar and SPECT showed grade 1 uptake The orbital activity ratios on planar imaging were not significantly different between complete and incomplete vascularization ($1.96{\pm}0.87$ vs $1.17{\pm}0.08$, p>0.05), however, it was significantly higher on SPECT in patients with complete vascularization ($8.44{\pm}5.45$ vs $2.20{\pm}0.87$, p<0.05). Conclusion: In the assessment of fibrovascular ingrowth within ocular implants by Tc-99m MDP bone scintigraphy, SPECT image appears to be more effective than planar scintigraphy.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.4
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• pp.257-267
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• 1993

Optimum pasture management during the summer season is an important factor to maintain good regrowth and persistence of pasture. The field experiment was carried out to investigate the effects of cutting management on growth and reserve carbohydrates in stubble. and on dry matter yield of orchardgrass dominated pasture during the mid-summer season. Three different cutting times(July 15, 25 and August 5) as a main plot and three cutting heights(3, 6 and 10cm) as a sub plot were disigned with three replications. The experiment was done at pasture of Changsung Agricultural High School in 1988. The results obtained were summarized as follows: 1. Temperature of soil surface and underground in the cutting of July 15 and July 25 was not showed significantly different, because of low intensity of radiation with a run of wet weather, and that of August 5 cutting tends to be lowed in high cutting height during the mid-summer season. 2. Amount of soil moisture against cutting height showed that high cutting had a low content. 3. Growth rate against regrowth of plant height, lear length and leaf area showed to be fast in high cutting. 4. Rapid recovery period of carbohydrate content after cutting and large amount of carbohydrate accumulation were showed in the cutting of July 15 and 25 compared with August 5 cutting in the reserve carbohydrate content against cutting of time and height during the mid-summer season. 5. Dry matter yield of the 4th and 5th cutting showed to be remarkable in July 15 cutting compared to those of July 25 and August 5. From the above results, it is suggested that the 10cm cutting height during the mid-summer season is the most effective for good regrowth, reserve carbohydrates and dry matter yield of orchardgrass.

• Journal of Mushroom
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• v.5 no.1
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• pp.39-42
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• 2007

This study was carried out to characterize virus-like particles (VLPs) in Pleurotus ostreatus. The white and the dark gray mutants frequently observed in mushroom farms of Pleurotus ostreatus (Wonhyeong-neutari). A 5.8kb segments of dsRNA was detected only in the white mutants but not in the dark gray mutants. The VLPs were purified from the fruit bodies by Polyethylene Glycol (PEG) and ultracentrifugation. Electron microscopy analysis showed that VLPs were isometric about 14, 20~45nm in diameter. Further study is needed to reveal the morphological and yield variations of mushroom strains including VLPs observed in the mushroom farms. Also it is needed to maintain fundamental research for taxonomy, diagnosis, and physiology of VLPs in the mushroom strains.

• Journal of Bio-Environment Control
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• v.13 no.3
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• pp.135-142
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• 2004

The objective of this study was carried out to determine which method is effective for improving seed germination in carrot and onion among osmotic or solid matrix priming. Seeds were osmotic primed with -0.5 MPa polyethylene glycol 8000 (PEG) solution and solid matrix primed with Micro Cel E (seed, Micro Cel E, and water mixed with the ratio of 4.0：2.0：8.0 by weight for carrot, and the ratio of 4.0：1.2：5.0 for onion at $20^{\circ}C$ for 4 days). Seeds were rapidly absorbed water after osmotic priming and solid matrix priming treatment. The moisture content of solid matrix primed seeds was lower than that of osmotic primed seeds in carrot and onion. Osmotic priming and solid matrix priming did not influenced on increasing of percent germination, but $T_{50}$ of treated seeds was shorter than those of untreated seeds at four temperature regimes. The effects of osmotic or solid matrix priming in reducing the $T_{50}$ was greater when the seeds were germinated at $15^{\circ}C$ than at $25^{\circ}C$ and $30^{\circ}C$ temperature. Solid matrix primed seeds germinated faster than osmotic primed seeds at all temperature in both carrot and onion. After priming, surface-dried seeds germinated faster than dried-back seeds in carrot and onion. Emergence speed of carrot and onion seeds was faster than untreated seeds by osmotic priming or SMP treatment although the final emergence percentage was not significantly influenced. On the other hand, early growth was not significantly influenced by osmotic priming or SMP treatment of carrot and onion seeds.

• Korean Journal of Food Science and Technology
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• v.22 no.6
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• pp.611-617
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• 1990

This study was conducted to breed a high vitamin $B_{12}$ producer by the fusion of protoplasts between Bacillus natto and Bacillus megaterium. Auxotrophic mutants of Bacillus natto SH-34 ($thr^-try^-rif^r$) and Bacillus megaterium BK-13 ($arg^-ade^-lys^-str^r$) which showed high protease activity and production of vitamin $B_{12}$, respectively, were isolated for the fusion experiment. Protoplasts were induced by incubating the cells with lysis solution containing $500{\mu}/ml$ lysozyme, and the ratio of protoplast and regeneration formation were ranged from 99% and 67%, respectively. Fusion frequencies of fusants between Bacillus natto SH-34 and Bacillus megaterium BK-13 were appeared in the ranges of $1.0{\times}10^{-5}$ under the treatment of 30% PEG 6000 containing 3% PVP. The fusant, MNF-72 showed the highest product yield of $7.85{\mu}g/g-cell\;vitamin\;B_{12}$ in production medium. For the improvement of productivity, the immobilization of fusants with sodium alginate was carried out. In batch and continuous fermentation systems, the productivity were determined to be $0.58{\mu}g/ml.hr\;and\;0.80{\mu}g/ml.hr\;vitamin\;B_{12}$ under optimum condition, respectivity.

• Membrane Journal
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• v.22 no.3
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• pp.224-233
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• 2012

To improve permeation performance of gas separation membrane, polysulfone hollow fiber membrane was prepared by wet-dry phase inversion method using Triton X-100 as non-ionic additive. And variation of gas permeation behavior by additive was investigated. Various spinning conditions such as air gap, concentration of polymer, dope tank temperature were controlled and these effects were studied. The morphology and gas permeation property of hollow fiber membranes were investigated using scanning electron microscope (SEM) and bubble flow meter respectively. We confirmed that the membranes added with Triton X-100 had a smooth external skin at various air gap length conditions. The macrovoids of these hollow fiber membranes were more developed with increase of air-gap from 4 to 90 cm and that induced higher permeance. The permeance of polysulfone membranes has the higher value at comparatively lower concentration polymer (30 wt% polysulfone) and lower concentration of additive (15 wt% Triton X-100). When temperature in dope tank was controlled, the membranes prepared at $100^{\circ}C$ showed low permeance because of volatilization of additive and solvent.

• The Journal of Korea Assosiation for Disability and Oral Health
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• v.12 no.2
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• pp.96-100
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• 2016

Metachromatic leukodystrophy (MLD) is a progressive and degenerative neurological disease caused by a deficiency of the catabolic enzyme arylsulfatase A. Deficiency of arylsulfatase A results in accumulation of sulfatide in the white matter of the peripheral and central nervous system and it occurs demyelination as a result. The patient gradually goes through mental and motor failure. General symptoms of MLD include gait disturbance, mental deterioration, muscle rigidity and impaired swallowing. Inheritance of the disease is autosomal recessive. We report a dental caries treatment of a 3-year old boy with MLD. The patient underwent hematopoietic stem cell transplantation (HSCT) to slow the progression of the disease. He was suffered from difficulties of mastication and swallowing from the degenerative neurological symptom. He was ingesting food by both oral feeding and tubal feeding after he took percutaneous endoscopic gastrostomy (PEG). The cause of multiple caries was mainly presumed as patient's prolonged time of meal. The treatment was performed under general anesthesia considering patient's incompliance. Severely affected lower primary molars were treated with pulp treatment and restored with stainless steel crown. Others were restored with composite resin. There were no postoperative complications. MLD is life threatening progressive disease and also has an impact on unfavorable condition for oral health. Routine home oral care and periodic professional dental care should be emphasized to the caregiver of patient considering the susceptibility of dental caries. Not only the medical care, but periodic dental office visit would benefit the quality of life of the patient.