• Journal of Microbiology and Biotechnology
• /
• 제18권5호
• /
• pp.958-963
• /
• 2008

A series of experiments were conducted to assess the effectiveness of rhizobacteria containing 1-aminocyclopropane-1-carboxylate (ACC) deaminase for growth promotion of peas under drought conditions. Ten rhizobacteria isolated from the rhizosphere of different crops (peas, wheat, and maize) were screened for their growth promoting ability in peas under axenic condition. Three rhizobacterial isolates, Pseudomonas fluorescens biotype G (ACC-5), P. fluorescens (ACC-14), and P. putida biotype A (Q-7), were selected for pot trial on the basis of their source, ACC deaminase activity, root colonization, and growth promoting activity under axenic conditions. Inoculated and uninoculated (control) seeds of pea cultivar 2000 were sown in pots (4 seeds/pot) at different soil moisture levels (25, 50, 75, and 100% of field capacity). Results revealed that decreasing the soil moisture levels from 100 to 25% of field capacity significantly decreased the growth of peas. However, inoculation of peas with rhizobacteria containing ACC deaminase significantly decreased the "drought stress imposed effects" on growth of peas, although with variable efficacy at different moisture levels. At the lowest soil moisture level (25% field capacity), rhizobacterial isolate Pseudomonas fluorescens biotype G (ACC-5) was found to be more promising compared with the other isolates, as it caused maximum increases in fresh weight, dry weight, root length, shoot length, number of leaves per plant, and water use efficiency on fresh and dry weight basis (45, 150, 92, 45, 140, 46, and 147%, respectively) compared with respective uninoculated controls. It is highly likely that rhizobacteria containing ACC deaminase might have decreased the drought-stress induced ethylene in inoculated plants, which resulted in better growth of plants even at low moisture levels. Therefore, inoculation with rhizobacteria containing ACC deaminase could be helpful in eliminating the inhibitory effects of drought stress on the growth of peas.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권10호
• /
• pp.1434-1439
• /
• 2001

Eighty crossbred pigs (Large White x Landrace) weighing 9.9 kg were assigned on the basis of sex, weight and Jitter to one of five dietary treatments in a factorial $(5\;treatments\;{\times}2\;sexes)$ arrangement to compare the nutritive value of hulled and dehulled peas fed with or without enzyme (0.25% Allzyme PF and 0.5% Biogal-S). A barley and soybean meal diet served as a control. Eight castrates and eight gilts were fed each diet. Digestibility coefficients for dry matter, crude protein and energy were higher for diets containing dehulled peas than hulled peas. In addition, enzyme supplementation modestly increased the digestibility of all three nutrients. Over the entire experimental period (9.9 to 103.3 kg), there were no performance differences (p>0.05) between pigs fed soybean meal based diets or diets based on any of the pea products. In addition, there were no differences in performance between pigs fed diets containing hulled or dehulled peas or between pigs fed diets with or without dietary enzyme. Castrates gained weight significantly faster, consumed more feed but had a poorer feed conversion than gilts (p<0.05). There were no differences in carcass traits between pigs fed diets based on soybean meal or any of the pea products. Carcass traits were similar for pigs fed hulled or dehulled peas while enzyme supplementation also had no effect on carcass data. Castrate pigs had a lower carcass value index, estimated lean yield and loin lean depth (p<0.05). Loin fat depth was greater for castrates than gilts (p<0.05). The overall results of this experiment provide little support for the need for enzyme supplementation of pea based diets fed to swine. In addition, dehulling did not appreciably improve the nutritive value of peas. Therefore, since the process adds to the cost of the raw product, its use is unlikely to be economical.

• 한국식생활문화학회지
• /
• 제20권6호
• /
• pp.761-766
• /
• 2005

완두콩을 시료로 하여 $5^{\circ}C,\;20{\circ}C$에서 저장하면서 저장기간에 따른 외관, 클로로필, 아스코르빈산의 함량 변화를 조사한 결과는 저장기간에 따른 완두콩의 품질변화는 꼬투리째 저장한 완두콩이 완두콩만을 저장한 경우보다 저장성이 좋은 것으로 나타났으며, 온도별로는 $5^{\circ}C$ 저장이 $20^{\circ}C$에서 저장했을 때보다 저장성이 좋은 것으로 나타났다. 클로로필의 함량변화는 5, 20의 저장조건 모두 꼬투리째 저장한 완두콩의 클로로필 함량이 많았다. 아스코르빈산 함량변화는 $20^{\circ}C$에서 급격히 감소하였으나, $5^{\circ}C$ 저장에서는 저장 21일째에 아스코르비산 함량이 $20^{\circ}C$ 저장 $2{\sim}7$일째의 함량과 비슷하게 나타났다. 위의 결과로 보아 완두콩의 저장성은 꼬투리째 저장하는 것이 완두콩만을 저장하는 것보다 더 좋으며, 실온보다는 약 5 에서 저장하는 것이 더 외관, 클로로필, 아스코르빈산의 함량 유지면에서 더 좋은 것으로 판단된다.

• 디지털융복합연구
• /
• 제17권5호
• /
• pp.385-390
• /
• 2019

본 연구는 도핑 (doping)에 대한 태도를 정량적으로 평가하기 위해 개발된 Performance Enhancement Attitude Scale (PEAS)의 다양한 버전에 대해 모형적합도 (model fit)를 확인함으로써 대한민국 엘리트선수에게 적합한 한국형 PEAS에 대한 정보를 제공하고자 하였다. 180명의 엘리트 육상선수를 대상으로 잘 훈련된 2명의 조사자 감독하에 17문항 PEAS를 자가기입식으로 응답하는 방법으로 자료를 수집하였고, 이를 활용하여 11문항과 9문항, 8문항 및 6문항으로 구분하여 모형적합도를 확인하였다. 그 결과, 8문항 PEAS가 대한민국 엘리트 육상선수에게 좋은 적합도를 보였고, 6문항 PEAS는 청소년선수에게 좋은 적합도를 보였으나 통계적으로 유의하게 나타나지는 않았다. 이러한 결과는 8문항 PEAS를 사용하는 것이 성인 및 청소년 육상선수의 도핑에 대한 태도를 측정하는 데에 더 타당하다는 것을 의미하고, 추후 이를 활용하여 도핑 행위에 영향을 미치는 심리사회학적 요인을 확인한다면 반도핑 전략 개발에 있어서 정확한 정보를 제공할 수 있을 것이다.

• 한국식품영양과학회지
• /
• 제19권3호
• /
• pp.195-200
• /
• 1990

Effects of cooking time(5-30 min in a pressure cooker) and HTST air dehydratiion time(0-9min at 15$0^{\circ}C$) on physical properties of dried green peas(3% oisture content wet basis) were investigated by determining rehydration ratio rehydration curve browning reaction and puncture force, The rehydration ratio and curve of dried green peas were increased with increa-sing cooking time and HTST air dehydration time. Preheating of the green peas for 30 min in a pressure cooker or for 9 min of HTST air dehydration time prior to 6$0^{\circ}C$ air dehydration recovered a 87.3% of original moisture content of raw green peas in a boiling water for 5 min. The brownin greaction was gradually decreased up to 15 min of cooking time. Puncture pressure of rehydrated green peas treated in a boiling water for 5 min was decreased as the cooking time and HTST air dehydration time were increased and was highly correlated with rehydration (r=-0.956) The effects of cooking time and HTST air dehydration time on rehydration ratio browning reaction and puncture pressure were significantly different at the a=0.01 level except effect of HTST air dehydration time on browning reaction.

• 디지털융복합연구
• /
• 제16권11호
• /
• pp.599-604
• /
• 2018

본 연구는 다양한 유형의 운동수행능력 향상에 대한 태도 척도 (Performance Enhancement Attitude Scale, PEAS)에 대해 확인적 요인분석 (confirmatory factor analysis)을 사용하여 요인구조 (factor structure)를 확인함으로써, 한국 엘리트 선수들에게 적합한 한국형 PEAS를 제공하고자 하였다. 355명의 핸드볼 선수를 대상으로 17문항의 PEAS를 자기기입식으로 조사하여 자료를 수집하였고, 17문항과 11문항, 9문항, 8문항 및 6문항에 대해 각각 확인적 요인분석을 시행하였다. 그 결과, 6문항의 PEAS가 한국 엘리트 운동선수들에게 적합한 것으로 확인되었고, 특히 청소년 선수에 비해 성인 선수에게 더 좋은 적합도를 보였다. 이러한 결과는 성인 선수의 도핑에 대한 태도는 6문항의 PEAS를 사용하여 더 정확하게 측정할 수 있을 것을 의미하고, 청소년 선수의 도핑에 대한 태도를 확인하기 위해서는 적합한 문항으로 구성된 설문지가 개발되어야 할 것이다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권1호
• /
• pp.102-105
• /
• 2002

Seventy-two crossbred pigs weighing an average of 41.5 kg were assigned on the basis of sex, weight and litter to one of four dietary treatments in a factorial (4 treatments${\times}$2 sexes) arrangement. The control diet was based on barley and soybean meal while the experimental treatments consisted of diets in which a portion of the dietary protein was supplied by 20% of a 50:50 blend of extruded ($130^{\circ}C$ for 20 to 25 sec) peas and full-fat canola seed, 20% of a 50:50 blend of unextruded peas and full-fat canola seed or a diet containing 10% peas, 6% canola meal and 4% canola oil (to equal the level of canola oil provided by 10% whole canola seed). Digestibility coefficients for dry matter, crude protein and gross energy were significantly higher (p<0.05) for the control diet than for the other three diets. Extrusion produced no beneficial effects (p>0.05) on nutrient digestibility and there were no differences in digestibility between the diet based on intact canola seed compared with the diet containing canola meal and oil. Choice of protein supplement had no significant effects on gain, feed intake or feed conversion during the grower or finisher phases and over the entire experimental period. Extrusion of the pea-canola blend produced no beneficial effects on pig performance as the performance of pigs fed either the extruded or unextruded blend of peas and canola seed was similar. In addition, the performance of pigs fed diets containing intact canola seed was similar to that of pigs fed canola meal and oil. Castrates gained faster and consumed more feed than gilts (p<0.05). However, their feed conversion was poorer than that of the gilts during the finisher period. There were no significant differences in carcass traits between pigs fed the control and any of the experimental treatments. Extrusion had no effect on carcass traits and the carcasses of pigs fed canola meal and oil did not differ from those of pigs fed whole canola seed. Castrates had a significantly lower dressing percentage, lower estimated lean yield but greater loin fat depth than gilts (p<0.05). The results of this experiment indicate that peas in combination with canola seed or canola meal are an acceptable alternative to soybean meal as a protein supplement for use in growing-finishing swine diets. Extrusion did not appear to have any beneficial effects on the nutritional value of the canola seed-pea blend as nutrient digestibility, growth performance and carcass traits were similar for pigs fed the unextruded blend of peas and canola seed compared with the extruded product. Since the process adds to the cost of the raw products, its use is unlikely to be economical.

• 전기화학회지
• /
• 제9권2호
• /
• pp.89-94
• /
• 2006

Nafion/poly(ether(amino sulfone)) acid-base 블렌드 고분자 전해질 막을 제조하여 이온전도도, 디메틸 에테르(DME) 투과도를 측정하였으며 이를 이용하여 직접 DME 연료전지 특성을 연구하였다. Poly(ether(amino sulfone)) (PEAS)는 아민기의 치환도가 $0.6\sim2.0$인 것을 합성하였다. Nafion/PEAS 블렌드 전해질 막은 Nafion과 PEAS를 DMF에 용해시켜서 캐스팅하는 방법으로 제조하였다. 블렌드 전해질막은 $100^{\circ}C$ 이상에서도 이온전도도가 계속 증가하였다. Nafion/PEAS-0.6(85:15) 블렌드 전해질막은 $50^{\circ}C$ 이상에서의 수소 이온전도도가 recast Nafion보다 더 높게 나타났으며 $120^{\circ}C$에서의 수소 이온전도도는 $1.42\times10^{-1}S/cm$로 측정되었다. PEAS의 아민기가 많이 치환될수록 블렌드 전해질막의 DME 투과도와 이온전도도는 비례적으로 감소하는 것을 확인할 수 있었다. $100^{\circ}C$ 이상 가압 조건에서 Nafion/PEAS 블렌드 전해질막을 사용한 직접 DME 연료전지(DDMEFC)의 최대 전력밀도가 같은 조건에서 Nafion 115를 사용한 것보다 약 50%증가하였다. 이와 같은 DDMEFC의 성능 향상은 블렌드 전해질막이 Nafion과 비교하여 고온에서의 이온전도도가 향상되었고 DME투과도가 감소하였기 때문인것으로 해석된다.

• 한국작물학회지
• /
• 제45권2호
• /
• pp.93-96
• /
• 2000

The present study was performed to obtain the basic information about growth and quality related characteristics and optimum harvesting time for podedible pea which is a new crop in Korea but believed to have a great deal of potentials for both domestic and overseas markets. They can be consumed either as a fresh succulent vegetable or as tender green pods. The daily green pod yield of pod-edible peas started to increase from ten days after flowering and the maximum yield was recorded on 26 days after flowering. Ninety percent of pod yields could be harvested from 16 to 36 days after flowering. Mean green pod yield for the tested varieties was approximately 8.0 t/ha. Total vitamin C content of pod-edible peas showed continuously decreasing trends from five days after flowering. The highest sucrose content was obtained at ten days after flowering. The highest panel score based on sweetness, chewiness, and hardness for the processed green pods was shown at 10-15 days after flowering in all varieties tested, indicating that the optimum harvesting time for pod-edible peas was considered to be 10-15 days after flowering.

• Mass Spectrometry Letters
• /
• 제15권2호
• /
• pp.79 -94
• /
• 2024

Over the past few decades, new psychoactive substances (NPS) have become prevailing. With the widespread emergence of NPS, phenethylamines (PEAs) have become one of the groups abused most which PEAs, along with other stimulants, make up the majority of stimulants. When determining the NPS, the methods for screening and confirmation are crucial which assesses the reliability of testimony. In this study, a set of GC/MS methods employing two derivatizing agents for determining 76 target PEAs in urine was established and further applied for authentic sample analysis. Five PEAs (N,N-DMA, PMMA, 4-CA, amphetamine, and methamphetamine) with contents over their LLOQs were detected in thirteen of the twenty tested samples. In order to compare the result from the GC/MS methods with the previously established LC-MS/MS method, Cohen's kappa coefficient and McNemar's test were applied for statistical analysis. Perfect agreement between GC/MS and LC-MS/MS techniques for determining target PEAs is demonstrated by the Kappa coefficient for each of the five detected targets.