• Applied Microscopy
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• v.31 no.4
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• pp.347-354
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• 2001

Immunoelectron microscopy of storage protein at early stage of seed development showed legumin was firstly accumulated protein in between endoplasmic reticulum (ER) cisternae, and these accumulates were differentiated into protein body (PB) by transformation at later stage. Thin sections of pea cotyledons during the later stages of seed maturation showed three morphologically different types of protein bodies. One of these, presented as rough-surfaced cisternae with terminal dilations, which contained protein deposits and were often found interdigitated between stacks of rough endoplasmic reticulum. Conventional electron microscopy at earlier stages of cotyledon development showed this protein body type initially developed from the rough ER. This transformation of endoplasmic reticulum into a protein body is believed to represent a new pathway of protein body development.

• Journal of Microbiology and Biotechnology
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• v.24 no.8
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• pp.1015-1025
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• 2014

Microorganisms that live in the rhizosphere play a pivotal role in the functioning and maintenance of soil ecosystems. The study of rhizospheric cyanobacteria has been hampered by the difficulty to culture and maintain them in the laboratory. The present work investigated the production of the plant hormone indole-3-acetic acid (IAA) and the potential of biofilm formation on the rhizoplane of pea plants by two cyanobacterial strains, isolated from rice rhizosphere. The unicellular cyanobacteria Chroococcidiopsis sp. MMG-5 and Synechocystis sp. MMG-8 that were isolated from a rice rhizosphere, were investigated. Production of IAA by Chroococcidiopsis sp. MMG-5 and Synechocystis sp. MMG-8 was measured under experimental conditions (pH and light). The bioactivity of the cyanobacterial auxin was demonstrated through the alteration of the rooting pattern of Pisum sativum seedlings. The increase in the concentration of L-tryptophan and the time that this amino acid was present in the medium resulted in a significant enhancement of the synthesis of IAA (r > 0.900 at p = 0.01). There was also a significant correlation between the concentration of IAA in the supernatant of the cyanobacteria cultures and the root length and number of the pea seedlings. Observations made by confocal laser scanning microscopy revealed the presence of cyanobacteria on the surface of the roots and also provided evidence for the penetration of the cyanobacteria in the endorhizosphere. We show that the synthesis of IAA by Chroococcidiopsis sp. MMG-5 and Synechocystis sp. MMG-8 occurs under different environmental conditions and that the auxin is important for the development of the seedling roots and for establishing an intimate symbiosis between cyanobacteria and host plants.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.637-647
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• 1993

Over 40 per cent Indian population needs to be attended for nutritional improvement. Traditional nutritious food resources though abundantly available but presently less used, if incorporated in the diets, can yield low-cost nutritionally balanced diets. The linear programming (LP)model was used for optimization of food resources to satisfy protein -energy requirements of hard working rural poor. Soybean-a highly nutritious food legume-available at reasonably low price was included along with chick -pea and pigeon -pea for comparison .The three cereals predominantly used in Indian diets -rice, wheat and sorghum -and three typical leafy vegetables namely, spinach , drumstick leaves and rajagira (Amaranths paniculantus) leaves were used for optimization . The contribution of food legume was restricted to supply only 50 per cent of daily protein requirement for maximum protein value. The quantities of other food stuffs namely , milk , sugar , tubers etc. were restricted to nati nal per capita availability. the nutrition satisfaction levels in per cent RDA by the model are 149-250 for protein , 51-106 for fat, 134-362 for iron an d143-1158 for Vitamin-A. Quantities of food legumes, cereals and leafy vegetables ranged from 289-601, 3000-3700 and 360-1200 g/day respectively for a reference family of five hard working rural poor. Daily food resources cost ranged from US$ 0.6 to 1.0 for the family. Amongst food legumes, soybean based diet combinations provide maximum nutritional benefits at minimum cost indicating its suitability for wider adoption by hard working poor.

• The Korean Journal of Ecology
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• v.19 no.2
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• pp.151-163
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• 1996

To investigate the early symptoms of light-chilling, alterations of chlorophyll fluorescence transients were monitored in cucumber (Cucumis sativus L. cv. Ilmichungjang) leaves. During 24 h chilling, decreases in (Fv)m/Fm, qE and qQ, and an increase in Fo were observed. The chilling effects were not recovered at room temperature, and a significant increase in Fo was observed during the recovery period. After 6 h chilling, ‘dip’(D) level of the transients became obscure, and the negative slope after ‘peak’(P) disappeared. The first derivative (dFv/dt) of the fast fluorescence rise curve was used to obtain more accurate information about the changes in the transients. The maximal rate of the fluorescence increase in the D-p rise curve (Fr) has been the most frequently used chilling stress indicator. However, a correct value of Fr could not be measured when the D level became obscure. This problem was overcome by introducing a new indicator, HFr (dFv/dt at Fv = 1/2 (Fv)m), and HFr gave very similar values to Fr. To monitor the changes in curvature around D level, another new parameter, ${\Delta}S$(D-Fr), was also introduced. These three parameters decreased very sensitively during light-chilling. In addition, increases in these parameters were observed during the first 2 h chilling, but this increase in Fr was also observed in pea leaf discs dark-chilled for 15 min, suggesting that this very early change is a common response to chilling in both pea and cucumber leaves. Quenching coefficients were also very sensitive to chilling, especially qE. Discussion on the usage of these parameters as chilling stress indicators is given in the text.

• Membrane Journal
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• v.21 no.3
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• pp.270-276
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• 2011

A nascent membrane was prepared by using the solution evaporation method with a solution of sPEEK, EdAn (cross-linking reagent), and PEA (grafting reagent) in DMAc. Then, after the imination and sulfonation process the cross-linked and grafted ion-exchange membrane, CG-sPEEK, was obtained. The sulfonation and imination reactions were confirmed by FTIR analysis. In order to evaluate the possibility of prepared membrane for the use of an ion-exchange membrane in PEMFC, proton conductivity, water uptake and volume change were measured and compared with a commercial membrane, Nafion 115. It was revealed that since the proton conductivity (0.17 S/cm) of prepared membrane were much higher than those of Nafion 115 (0.10 S/cm) the prepared membrane could be used for the ion-exchange membrane in PEMFC. However, the high water uptake (130%) of CG-sPEEK should be reduced for the dimension stability.

• Toxicological Research
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• v.34 no.3
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• pp.267-279
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• 2018

Neurolathyrism is a neurodegenerative disorder characterized by spastic paraplegia resulting from the excessive consumption of Lathyrus sativus (Grass pea). ${\beta}$-N-Oxalyl-L-${\alpha},{\beta}$-diaminopropionic acid (L-ODAP) is the primary neurotoxic component in this pea. The present study attempted to evaluate the proteome-wide alterations in chick brain 2 hr and 4 hr post L-ODAP treatment. Proteomic analysis of chick brain homogenates revealed several proteins involved in cytoskeletal structure, signaling, cellular metabolism, free radical scavenging, oxidative stress and neurodegenerative disorders were initially up-regulated at 2 hr and later recovered to normal levels by 4 hr. Since L-ODAP mediated neurotoxicity is mainly by excitotoxicity and oxidative stress related dysfunctions, this study further evaluated the role of L-ODAP in apoptosis in vitro using human neuroblastoma cell line, IMR-32. The in vitro studies carried out at $200{\mu}M$ L-ODAP for 4 hr indicate minimal intracellular ROS generation and alteration of mitochondrial membrane potential though not leading to apoptotic cell death. L-ODAP at low concentrations can be explored as a stimulator of various reactive oxygen species (ROS) mediated cell signaling pathways not detrimental to cells. Insights from our study may provide a platform to explore the beneficial side of L-ODAP at lower concentrations. This study is of significance especially in view of the Government of India lifting the ban on cultivation of low toxin Lathyrus varieties and consumption of this lentil.

• The Plant Pathology Journal
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• v.18 no.3
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• pp.130-137
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• 2002

Potato virus Y (PVY) is one of the most important viruses in many field crops in Korea. In this study, 31 PVY isolates were isolated from infected potato (Solanum tuberosum), tobacco (Nicotiana tabacum), pea (Pisum sativum), and weeds (Veronica persica, Lamium amplexicause and Capsella bursa-pastoris) showing different mosaic symptoms in Jeonbuk, Chungnam, Gangwon, and Gyeongbuk areas in Korea. The 640 nucleotide region containing the C-terminal portion of coat protein (CP) gene and 3'non-translated region (NTR) was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using PVY-specific oligonucleotide primers. Sequence analyses of the amplified DNA fragments showed that the C-terminal portion of CP gene was not significantly different from that of previously reported PVY strains from potato (PVY-OK and -T) and tobacco (PVY-VN) in Korea. Homologies of the deduced CP amino acid sequences were 93.3-99.0% to corresponding regions of the other PVY strains including PV $Y^{N}$, PV $Y^{o}$ , PV $Y^{OK}$ , PV $Y^{T}$ , and PV $Y^{VN}$ . In contrast the sequences located at the 3'-NTR showed more diverse sequence homologies (76.4-99.7%). These results indicate that the C-terminal portion of the CP gene was relatively conserved while sequences at the 3'NTR were more diverse and variable over the host species and the regions where they were isolated.e isolated.

• Applied Biological Chemistry
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• v.32 no.2
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• pp.154-161
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• 1989

1,3-Dimethyl-4-acyl-5-hydroxypyrazoles were treated with methyl isocyanate to give corresponding carbamates. They were 1,3-dimethyl-4-benzoyl-pyrazol-5-yl N-methylcarbamate(V), 1, 3-dimethyl-4-(2-chlorobenzoyl)-pyrazol-5-y1 N-methyl-carbamate(VI), and 1, 3-dimethyl-4-(2,4-dichlorobenzoy1)-pyrazol-5-yl N-methyl-carbamate(VII). The structures of the compounds were fully identified by UV, IR, $^{1}H-NMR$ and $^{13}C-NMR$. The synthesized carbamate compounds were tested for the phytotoxicity on the seed germination and the seedling growth of rice, radish, green-pea and turf grass. At 50ppm concentraction, the germination of seeds was not significantly affected. At higher concentraction, however, phytotoxic tendency on the seed germination and seedling growth was observed. In addition, these compounds were less phytotoxic to rice and turf seedlings as compared with radish and green pea seedlings.

• Korean Journal of Environmental Agriculture
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• v.17 no.1
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• pp.39-42
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• 1998

In order to evaluate the biological activities of some fractions from the fungal(Pleurotus sajorcaju) fermentation products of sugar cane bagasses, the antimutagenicity, the glyceollin elicitor activity on soybean and the effect on the stem elongation in pea were observed. The alcohol extract fraction and DMSO soluble fraction had excellent antimutagenicity even though it is weaker than that of the extracts from the fruiting bodies. All of the extracts had the ability to elicit glyceollins in soybean cotyledons and these extracts could be helpful for plants to protect themselves from pathogenic contaminations. IAA and the extracts had shown synergistic effects on pea stem elongation in all experimental groups positively determined.

• Korean Journal of Community Nutrition
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• v.13 no.6
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• pp.829-839
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• 2008

The purpose of this study was to investigate the relation of the fast food and beverage intake on sociality and anger expression of adolescents. Questionnaires were distributed to the adolescents of 599 middle and high school students in Busan. According to the results, the preference-intake frequency analysis (PEA) on fast food grid, high preference and high intake frequency were 'dukbokki', 'chicken' and 'mandu' and low preference and high intake frequency were 'ramyon', 'gimbab. PEA on beverage grid, high preference and high intake frequency were 'milk-dairy product', 'fruit juice', 'isotonic beverage' and low preference and high intake frequency were 'carbonate drink'. The intake frequency of 'pizza', 'sandwich', 'udong', and 'dukbokki' had a positive relationship with sociality. 'Hamburger', 'chicken', 'french fry', 'gimbab', 'mandu', and 'ramyon' showed a positive relationship with anger-out. The intake frequency of 'carbonated drink' had a negative relationship with anger-control, but 'green tea' showed a positive relation with it. 'Carbonate drink', 'isotonic beverage', 'coffee', and 'milkshake' had a negative relationship with anger-out. The explanation power ($R^2$) of intake of fast food and beverage on sociality was $0.019{\sim}0.038$, and 'carbonated drink' and 'coffee' had a negative influence on sociality. The explanation power ($R^2$) of intake of fast food and beverage on anger expression was $0.011{\sim}0.041$, and 'carbonated drink' had a negative influence on angercontrol. 'Hamburger', 'carbonated drink', and 'coffee' showed a positive influence on anger-out. From these results, it was necessary to develop the practical eating-out habits program on proper fast food and beverage choice for adolescents.