• Title/Summary/Keyword: Pe

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Effects of Ethanol and Dietary Protein Levels on Calcium and Phosphorus Metabolism in Rats (알콜과 단백질수준이 흰쥐의 칼슘 및 인의 대사에 미치는 영향)

  • Koh, Jin-Bog;Han, Jae-Kum
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.2
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    • pp.229-237
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    • 1989
  • The effects of ethanol and dietary protein levels on the calcium and phosphorus metabolism were investigated in 15-week-old male rats. Rats were divided into 4 groups ; control group(16% protein, 16 PC) and 8%(8 PE), 16%(16 PE), and 24% protein groups (24 PE) to which was given 5% ethanol mixed with their drinking water. Body weigh gain, organ weight, hemoglobin content, and hematocrit value were not affected by either ethanol or dietary protein levels. Calcium concentrations in spleen were significantly decreased in the ethanol groups than those in control group. Calcium and Phosphorus levels in femur, serum, liver, kidney, and muscle were normal. Among ethanol treated groups, fecal excretions of calcium were a little more decreased, but urinary excretions, balances and apparent absorption rate of calcium were a little more increased in higher percentage of protein group than lower percentage. Urinary phosphorus excretions in the ethanol treated groups were significantly decreased compared with the control group. Among ethanol treated groups, phosphorus balance and apparent phosphorus absorption rate of 24 PE group were significantly higher than those of 8 PE and 16 PE groups.

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Fabrication and Evaluation of Si3N4-coated Organic/inorganic Hybrid Separators for Lithium-ion Batteries (Si3N4-코팅 유/무기 복합 분리막을 통한 리튬이온전지용 분리막의 제조 및 평가)

  • Yeo, Seung-Hun;Son, Hwa-Young;Seo, Myeong-Su;Roh, Tae-Wook;Kim, Gyu-Chul;Kim, Hyun-Il;Lee, Ho-Chun
    • Journal of the Korean Electrochemical Society
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    • v.15 no.1
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    • pp.48-53
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    • 2012
  • Polyethylene (PE) separator is the most popular separator for lithium-ion batteries. However, it suffers from thermal contraction and mechanical rupture. In order to improve the thermal/mechanical dimensional stabilities, this study investigated the effects of $Si_3N_4$ coating. SCS (Silicon-nitride Coated Separator) has been fabricated by applying 10 ${\mu}m$-thick $Si_3N_4$/PVdF coating on one side of PE separator. SCS exhibits enhanced thermal stability over $100{\sim}150^{\circ}C$: its thermal shrinkage is reduced by 10~20% compared with pristine PE separator. In addition, SCS shows higher tensile strength than PE separator. Employing SCS hardly affects the C-rate performance of $LiCoO_2$/Li coin-cell, even though its ionic conductivity is somewhat lower than that of PE separator.

Effect of pretreatment and packaging methods on quality of cold vacuum dried peach (전처리 및 포장방법이 냉풍감압건조 복숭아의 품질에 미치는 영향)

  • Kwon, Gi-Man;Kim, Jae-Won;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.20 no.3
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    • pp.317-322
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    • 2013
  • This study was conducted to investigate the effects of pretreatment and different packaging methods on the physicochemical properties of cold vacuum-dried peaches. All the dried peach samples were stored such as $N_2$ gas substitution, vacuum and passive packaged with polyethylene (PE) film and oriented polypropylene (OPP)/aluminum (Al)/PE film at $40^{\circ}C$ for 50 days. The weight change, pH, soluble solids content, titratable acidity, soluble solid-acid ratio (SS/TA), delta E, browning degree and phenolic compounds were analyzed. The weight change and pH were lower in the 0.1% vitamin C-treated group and were significantly lowest in the vacuum-treated OPP/AL/PE. The soluble solids content and the SS/TA were higher in the non-treated groups than in the vitamin C-treated groups. According to the packaging methods, the $L^*$ values were higher in the vacuum, $N_2$ gas and passive package, in that order. In addition, the browning degree and the delta E value were lower in the pretreated groups and significantly lowest in the vacuum-treated OPP/AL/PE with 0.1% vitamin C group. The phenolic compounds were high for the pretreated groups, according to the packaging methods (vacuum > $N_2$ gas> passive), and the OPP/AL/PE was significantly higher than the PE. These results suggest that different packaging materials and pretreatment methods affected the quality of the dried peaches, and the vacuum-OPP/AL/PE film packaging group showed a high quality.

Effects of $CO_2$ Absorbent in the PE Film Bag and Styrofoam Box during the Ginger Storage (PE 필름과 스치로폴 상자를 이용한 생강저장시 탄산가스 흡착제의 효과)

  • 최윤희;김명숙
    • Food Science and Preservation
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    • v.8 no.3
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    • pp.286-290
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    • 2001
  • Ginger was stored in the 0.05mm and 0.08mm of PE film bags and the styrofoam box. During the ginger storage, weight losses in the PE film bags were effectively supressed than in the control bag, and storage was better in the 0.08mm PE film bag while the spoilage of ginger was higher than in 0.05mm bag. The effective storage temperature was 10$^{\circ}C$ at which was no scouting during the storage. And the perforation in the PE film bag helped the healthy ginger ratio. Single perforation of 6an diameter was good for 0.05mm film and three for 0.08mm film. When CO$_2$absorbent was added into the film bag, the spoilage and mold occurence was lowered. In the styrofoam box, the healthy ginger ratio also increased by incorporating the absorbents, but there were no great differences between activated charcoal and calcium hydroxide.

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Cloning and Sequencing of the pelCl Gene Encoding Pectate Lyase of Erwinia carotovora subsp. carotovora LY34 (Erwinia carotovora subsp. carotovora LY34에서 pelCI 유전자 클로닝)

  • Lim, Sun-Tech;Park, Yong-Woo;Yun, Han-Dae
    • Applied Biological Chemistry
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    • v.40 no.5
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    • pp.380-387
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    • 1997
  • Phytopathogenic Erwinia carotovora subsp. carotovora (Ecc) LY34 causes plant tissue maceration by secretion of pectinolytic enzymes such as pectate Iyase (PL) existed as multiple isoenzyme form. Genomic DNA from Ecc LY34 was digested with Sau3Al and ligated into the BamHI site of pBluescript ll $SK^+$. Among them, a clone hydrolyzing polypectate was selected and its DNA was digested with BamHI. Through the subsequent subcloning the resulting 3.1 kb fragment, corresponding to a peICI, was subcloned into pLYPA 100. The structural organization of a peICI gene encoding a 374 amino acid residues consists of an open reading frame (ORF) of 1,122 bp commencing with a ATG start codon and followed by a TAA stop codon. PeICI contained a typical prokaryotic signal peptide of 22-amino acid. Since the deduced amino acid sequences of PeICl protein was very similar to those of PelIII of Erwinia carotovora subsp. carotovora, and to those of Pel3 of Erwinia carotovora subsp. atroseptica, and to those of PeIC of Erwinia carotovora subsp. carotovora, it belong to the same family PLbc group. The 374-amino acld PeICI had a calculated Mr of 40,507 and pI of 7.60.

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Processing and MAP(modified atmosphere packaging) Storage of Fresh-cut Apples using CA Stored Apples (CA저장 사과를 이용한 Fresh-cut Apple의 제조 및 MAP저장)

  • 정헌식;문광덕;최종욱
    • Food Science and Preservation
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    • v.6 no.4
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    • pp.351-356
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    • 1999
  • To develop and extend the shelf-life of vitamin C enriched fresh-cut apples using CA stored Fuji apples, dipping in 10% L-ascorbic acid and packaging with 0.03 mm LDPE, 0.04 mm PP and 0.08 mm Nylon/PE film(N$_2$displacement) were carried out. The changes of gas concentrations in the packaging and quality attributes of fresh-cut apples were examined during storage at 10$^{\circ}C$. The concentrations of O$_2$was maintained lower in Nylon/PE film than the other film, the level of O$_2$was in the range of 1∼3%. The increase of C$_2$H$_4$ concentrations in Nylon/PE film bag was more suppressed than the others. The vitamin C content of fresh-cut apples was enriched by dipping in L-ascorbic acid solution up to 241 mg$.$100 g-1 f.w., and the loss or that content was retarded differently by the package conditions of lower O$_2$level during storage. Browning in fresh-cut apples was shown after 6 days of storage in LDPE and PP film. but it was not shown by 14 days of storage in Nylon/PE film. Spoilage and off odor in fresh-cut apples were not detected up to 14 days of storage in Nylon/PE film. The results indicated that the vitamin C enriched fresh-cut apples can be processed from the long-term CA stored apples, and maintaining high quality of the products ill be possible in cases of the application of sealing packaging after O$_2$removal with film having low O$_2$permeability.

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Codon Optimization, Soluble Expression and Purification of PE_PGRS45 Gene from Mycobacterium tuberculosis and Preparation of Its Polyclonal Antibody Protein

  • Xu, Tao;Li, Minying;Wang, Chutong;Yuan, Meili;Chang, Xianyou;Qian, Zhongqing;Li, Baiqing;Sun, Meiqun;Wang, Hongtao
    • Journal of Microbiology and Biotechnology
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    • v.31 no.11
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    • pp.1583-1590
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    • 2021
  • Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

Dysregulation of Cellular Immune Functions on Gastric Administration to Mixtures of Polyethlene Microplastics and Metallic Lead in Mice (폴리에틸렌 미세플라스틱과 납의 복합노출에 따른 실험동물의 세포면역기능 조절 장애 평가)

  • Gyoungwoo Lee;Changyul Kim
    • Journal of Environmental Health Sciences
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    • v.49 no.1
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    • pp.22-29
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    • 2023
  • Background: The existing research results on the combined toxicity of these pollutants using mammals, such as rodents, are insufficient, especially in relation to changes in the immune system. Objectives: This study aims at evaluating the cellular immune response to PE-MPs solely or when combined with Pb, which possess excellent adsorption capacity with PE-MPs and is commonly co-exposed in our daily lives. Methods: The study investigated the cellular immune function of 9-week ICR mice with 28 days exposure to PE-MPs (2 mg/mouse/day) and Pb (0.1 mM in distilled water) individually and in combination. PE-MPs were administered via gastric intubation while the lead intake was conducted via the oral drinking water route. Cellular immunity was evaluated by analyzing the production for TH1 cytokines namely, TNF-α and IFN-𝛾 and TH2 cytokines, IL-4 and IL-6 in culture supernatants from polyclonally activated splenic mononuclear cells ex vivo. Results: Both the PE-MPs only and the PE-MPs+Pb exposure group revealed an increased TH1 response with elevated TNF-α and IFN-𝛾 levels and downregulated TH2 response with low IL-4, and IL-6 production levels compared to the control group. Furthermore, an increased IFN-𝛾/IL-4 ratio was found in the PE-MPs only and PE-MPs+Pb exposure groups, which indicated the skewedness to TH1 response. Meanwhile, reduced blood hemoglobin levels and increased levels of IL-4, the dominant TH2 cytokine in the Pb-only exposure group, were observed. Conclusions: Our current findings on the predominance of TH1 immune response in the PE-MPs and PE-MPs+Pb groups suggest that PE-MPs could be responsible for the predominant induction of the cellular immune changes. This finding could be used as an important landmark in research related to TH1 predominance, such as autoimmune diseases. It suggests that additional research on immune modulation using longer exposure durations or the same exposure route is required to elucidate stronger findings.

The Nutrition Knowledge, Attitudes, and Dietary Habits of Physical Education Majors and Non-majors of Male College Students (체육전공 남학생과 비전공 남학생의 영양지식, 식태도 및 식습관에 관한 연구)

  • Park, Hyun-Ock;Hyun, Hwa-Jin;Song, Kyung-Hee
    • Korean Journal of Community Nutrition
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    • v.14 no.4
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    • pp.363-373
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    • 2009
  • This study was performed to investigate the nutrition knowledge, attitudes, and dietary habits of physical education (PE) major and non-majors of male college students. The subjects were 253 students residing in Gyeonggi area. Nutrition knowledge scores of PE major students were lower than that of non-major students. Both PE major and non-major students reported TV, radio, and internet as their primary sources of nutrition information. Overall dietary attitude scores of two groups did not differ significantly, but PE major students showed more positive attitudes than non-major students for 'Enjoy eating foods that are good for health'. PE major students took more number of meals a day and took more irregularly than non-major students. Also they used more nutrient supplements than non-major students. PE major students drank more amounts of alcohol and drank more frequently than non-major students. But the smoking status of the two groups was not significantly different. PE major students scored higher than non-major students in dietary habits on balance of meals in spite of their low nutrition knowledge scores. Therefore, it is suggested that nutritional programs for PE major and non-major college students should be developed. (Korean J Community Nutrition 14(4) : $363{\sim}373$, 2009)

Effect of Mulching Material and Planting Density on Growth and Bulb Development of Shallot (Allium cepa var. ascalonicum Backer) (멀칭재료와 재식밀도가 Shallot(Allium cepa var. ascalonicum Backer)의 생장과 구의 발육에 미치는 영향)

  • Cho, Yong-Cho;Lee, Jong-Tae;Park, Yoo-Gyeong;Jeong, Byoung-Ryong
    • Korean Journal of Plant Resources
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    • v.24 no.5
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    • pp.507-513
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    • 2011
  • This study was conducted to evaluate the effect of mulching material and planting density on growth and bulb development of shallot. The transparent PE film was better than black PE film as a mulching material for the overwintering shallot crop. Transparent PE film mulching promoted plant growth and increased marketable yield by 21% as compared with that of the black PE film-mulched crop. However, the bulb size was not significantly affected by the type of mulching film. The height and width of ridge and planting density significantly affected the growth and bulb yield of the moisture sensitive shallot. Bulb yield of the shallot planted in five rows in 120 cm wide ridges (20,833 plants per 10 a) was 1,332 kg per 10 a, which was 1.7 times as high as that by the crop grown in three rows in 120 cm wide ridges (12,500 plants per 10 a). In conclusion, the shallot crop is recommended to be cultivated in five rows in 120 cm ridges mulched with the transparent PE film.