• 대한한의학회지
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• 제23권1호
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• pp.67-82
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• 2002

Objectives: In recent years, extensive focus has been laid on adulteration of herbal medicine with heavy metals. This may be mainly due to soil contamination by environmental pollution. The objective of this study is to identify the contents of various heavy metals in the blood from Ohjeok-san (Wuji-san) Decoction (OD) treated-rats. Methods: For this study, 13 kinds of metals including essential and heavy metals, i.e. A1, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se and Zn were analyzed by a slight modification of EP A methods and the following results are obtained. Results: 1. There was no significant difference between the OD-treated groups and control group in liver, kidney, bone, brain and weight, especially no significant difference at the 5th and 10th days in weight and the amount of food intake. 2. The amount of each metal analyzed in the blood were as follows; A1: 2.3~3.07 mg/l, As: 2.90~3.66 mg/l, Cd: 0~0.001 mg/l, Co: 0~0.01 mg/l, Cr: 0.40~043 mg/l, Cu: 0.93~1.88 mg/l, Fe: 414.35~464.46 mg/l, Hg: 0.01 mg/l, Mn: 0.10~0.17 mg/l, Ni: 0.01 mg/l, Pb: 0.03~012 mg/l, Se: 0.73 mg/l, Zn: 3.41~4.13 mg/l by groups, respectively. In control and experimental group, Experimental I and other experimental II, III, IV, and V groups, there were no significant differences. 3. The amount of non-toxic metals (A1, Co, Cu, Fe, Mn, Se, Zn) were $64.1{\pm}7.71{\;}mg/l$ in the control group and 60.70~67.58 mg/l in the experimental groups I, II, III, IV and V. The amount of Toxic metals (As, Cd, Cr, Ag, Pb) were $0.68{\pm}0.21{\;}mg/l$ in the control group and 0.57 ~ 0.66mg/l in the experimental groups. The total amount of metals were 32.35 mg/l in the control group and 30.48~34.12 mg/l in the test groups I, II, III, IV and V, respectively. Conclusions: There was no significant difference of metal concentration in the blood from the OD-treated-rats compared to those of the control group even if higher dosage (1~8 times the dosage for a person) of OD was administered. This may be mainly due to a decoction treatment which contains only supernatants filtered from the herb-mass after boiling. This indicates the legal limitation for metal concentration in herbal medicine must be applied according to different treatment methods of herbal medicine.

• 분석과학
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• 제11권4호
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• pp.271-280
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• 1998

국내에서 재배 생산된 현미로 부터 표준물질을 가공하여 원소별 검정분석을 실시하였다. 주요 오염원소의 농도가 서로 다른 2종의 쌀분말 표준물질을 제조하였는데 1종은 정상치시료로서 분말 시료를 그대로 처리하였으며, 1종은 제조과정중에 As, Cu, Pb, Hg, Cr, Cd등 6개의 중금속원소를 건조중량기준 $1.0{\mu}g/g$씩 첨가한 고농도 오염시료로서 제조하였다. 제조된 시료는 중성자방사화분석법 (NAA)과 불꽃원자흡수분광법(FAAS)으로 Cd, Cu, Fe, Mn, Zn등 5개 원소에 대한 균질도 분석을 실시하여 기준불질로서의 균질성을 확인하였다. 쌀시료의 분해 및 전처리에는 고온가압법과 마이크로파분 해법을 사용하였으며, NAA, FAAS, 흑연로원자흡수분광법(GFAAS), 유도플라즈마방출분광법(ICP-AES), 동위원소희석질량분석법(IDMS), 증기발생법 등을 이용하여 쌀분말표준물질 소재의 원소별 정량 분석을 실시하였다. 이들 각 방법의 원소별 분석결과를 이용하여 P, K, Mg, As, Ca, Cd, Cr, Cu, Fe, Mn, Mo, Na, Zn, Pb, Se, Hg등 16개 원소에 대한 검정값 및 참고값을 확정하였다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제10권1호
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• pp.43-57
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• 2005

주암댐에 유입된 호저퇴적물의 입도(2mm-200{\mu}m,\;200-100{\mu}m,\;100-50{\mu}m,\;50-20{\mu}m,\;<20{\mu}m)별 중금속(As, Cd, Cr, Cu, Ni, Pb, Zn)의 이동과 퇴적 후 중금속의 수직적 분산 특성을 규명하기 위하여 12개 지점의 호저퇴적물과 3개 지점의 코아시료를 채취하였으며, 코아시료는 2-5 cm 간격으로 절단하여 깊이별 간극수와 퇴적물의 중금속 함량을 측정하였다. 호저퇴적물의 퇴적속도를 측정하기 위하여 코아시료에 대한 $^{210}Pb$ 동위원소 연구를 수행하였다. 주암댐 호저 퇴적물의 중금속 및 미량원소의 함량은 퇴적위치와 상관없이 거의 일정한 함량을 보이며.,평균 함량은 $As\;14.9\;{\mu}g/g,\;Cd\;0.81\;{\mu}g/g,\;Cu\;30.7\;\;{\mu}g/g,\;Ni\;34.7\;{\mu}g/g,\;Pb\;63.3\;{\mu}g/g,\;Zn\;87.9\;{\mu}g/g$이다. Cu, Pb, Zn, Ni 및 Cd의 함량은 $20\;{\mu}m$ 이하 입도의 퇴적물에서 가장 높은 반면, As는 $2mm-200{\mu}m$ 혹은 $200-100\;{\mu}m$ 입도의 퇴적물에서 가장 높다. 간극수에 용해된 원소 중에서 가장 함량이 높은 것은 Fe와 Mn 이었으며, 코아시료의 상부로부터 심부로 갈수록 함량이 증가하는 수직적 분산특성을 나타낸다. 반면에 용존 Zn과 Cu함량은 코아시료의 최상부에서 가장 높게 나타나며, 이는 산화:환원 전위차에 따른 유기물질 및 산화광물의 용해작용과 관련이 있다. 코아시료의 퇴적물 내 Cu, Ni, Pb 및 Zn 함량은 0-4 cm구간에서 가장 높은 표면집적 현상이 관찰되며, Pb와 Cu 함량이 현저하게 높은 데, 이는 인위적인 오염에 기인하는 것으로 해석된다. $'unsupponed\;^{210}Pb'$을 이용하여 계산된 호저퇴적물의 퇴적 속도는 $\omega=0.91\;cm\;year^{-1}$ 이며, 이를 적용하면 주암댐이 완공된 이후 약 10 cm의 퇴적물이 평균적으로 퇴적된 것으로 추정된다. 이러한 자료는 주암댐 호수 바닥에 퇴적된 퇴적물의 관리방안을 마련하는 데 중요한 정보로 사용될 수 있을 것이다.

• 한국전문물리치료학회지
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• 제18권3호
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• pp.1-7
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• 2011

The effects of an abdominal drawing-in maneuver (ADIM) using a pressure bio-feedback unit (PBFU) were compared to the effects of a pelvic belt (PB) on the muscle activities of the hip and back extensor muscles during hip extension in the prone position. Fifteen healthy male participants all performed prone hip extensions under three conditions: 1) preferred hip extension (PHE), 2) performing an ADIM, and 3) using a PB. The muscle activities of the erector spinae, the gluteus maximus, and the medial hamstring on the right side were recorded by surface electromyography. The muscle activity of the erector spinae was significantly lower while performing an ADIM during prone hip extension than during PHE or with a PB (p<.05). Gluteus maximus muscle activity was significantly higher while performing an ADIM (p<.05). No significant difference was found for the medial hamstring muscle among the three conditions (p>.05). We concluded that the internal stabilization of the pelvis and lumbar spine afforded by the ADIM using a PBFU could be more effective than the external stabilization provided by a PB in terms of increasing selectively gluteus maximus activation during prone hip extension.

• Clinical and Experimental Reproductive Medicine
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• 제24권1호
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• pp.67-81
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• 1997

To determine the concentration and the physiologic role of metal components in blood plasma and seminal plasma in relation to male infertility, the concentrations of twelve metal components in blood plasma and seminal plasma including Na, Mg, K, Ca, Cr, Mn, Fe, Cu, Zn, Se, Cd and Pb were measured by atomic absorbance spectrophotometery or ion selective electrode analysis. Semen and blood samples were obtained from a total of 110 men including 70 male infertility patients, 20 vasectomized persons and 20 fertility proven volunteers visited to the Male Infertility Clinic of Pusan National University Hospital. The concentrations of Ca, Zn, Mg, Cr and Cd in control group were higher in seminal plasma than in blood plasma, and additionally Pb were higher in infertility group. The concentrations of all metal components revealed no significant difference according to patients' age, resident, occupation, sperm density, motility and hormone level in blood plasma, but some metal components including Ca, Mg, Cu, Mn, Cd and Pb revealed a significant difference according to each these parameters except patient's age in seminal plasma. The concentrations of Mn, Cd and Pb in the vasectomy persons were higher than in the infertility group III including testicular and epididymal factors, but not in blood plasma. We conclude that the quantitative changes of metal components in the seminal plasma may have effects on not only spermatogenesis and sperm function, but also contribute to diagnostic parameter according to organ specificity of the metal in the male reproduction.

• 한국광물학회지
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• 제11권1호
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• pp.20-31
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• 1998

Adsorption of metal elements onto illite and halloysite was investigated at $25^{\circ}C$ using pollutant water collected from the gold-bearing metal mine. Incipient solution of pH 3.19 was reacted with clay minerals as a function of time: 10 minute, 30 minute, 1 hour, 12 hour, 24 hour, 1 day, 2 day, 1 week, and 2 week. Twenty-seven cations and six anions from solutions were analyzed by AAs (atomic absorption spectrometer), ICP(induced-coupled plasma), and IC (ion chromatography). Speciation and saturation index of solutions were calculated by WATEQ4F and MINTEQA2 codes, indicating that most of metal ions exist as free ions and that there is little difference in chemical species and relative abundances between initial solution and reacted solutions. The adsorption results showed that the adsorption extent of elements varies depending on mineral types and reaction time. As for illite, adsorption after 1 hour-reaction occurs in the order of As>Pb>Ge>Li>Co, Pb, Cr, Ba>Cs for trace elements and Fe>K>Na>Mn>Al>Ca>Si for major elements, respectively. As for halloysite, adsorption after 1 hour-reaction occurs in the order of Cu>Pb>Li>Ge>Cr>Zn>As>Ba>Ti>Cd>Co for trace elements and Fe>K>Mn>Ca>Al>Na>Si for major elements, respectively. After 2 week-reaction, the adsorption occurs in the order of Cu>As>Zn>Li>Ge>Co>Ti>Ba>Ni>Pb>Cr>Cd>Se for trace elements and Fe>K>Mn>Al, Mg>Ca>Na, Si for major elements, respectively. No significant adsorption as well as selectivity was found for anions. Although halloysite has a 1:1 layer structure, its capacity of adsorption is greater than that of illite with 2:1 structure, probably due to its peculiar mineralogical characteristics. According to FTIR (Fourier transform infrared spectroscopy) results, there was no shift in the OH-stretching bond for illite, but the ν1 bond at 3695 cm-1 for halloysite was found to be stronger. In the viewpoint of adsorption, illite is characterized by an inner-sphere complex, whereas halloysite by an outer-sphere complex, respectively. Initial ion activity and dissociation constant of metal elements are regarded as the main factors that control the adsorption behaviors in a natural system containing multicomponents at the acidic condition.

• 한국환경농학회지
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• 제41권3호
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• pp.185-190
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• 2022

BACKGROUND: Most of the bottom ash(BA) from wood pellet-based thermal power plants that is not recycled is placed into landfill. BA has a function and structure similar to biochar. Hence, BA is classified as waste, but, it is predicted that BA can be used agricultural utilization. METHODS AND RESULTS: To investigate the effect of BA application on lettuce, growth characteristics and Pb contents were examined with BA application levels(0, 1, 2, 3 and 4 g/L), respectively, in hydroponic cultivation with Pb solution. Irrespective with BA application levels, the length, leaf number and fresh weight of lettuce in BA treatments were increased by 84.3~120, 36.2~39.0, and 215~322%, respectively, compared to the BA-0 treatment. The groups with BA treatments, Pb in the nutrient solution was adsorbed to the BA due to the surface area and functional groups of the BA, and the lettuce growth was maintained more smoothly than in the BA-0 treatment. BA application is considered to have created a favorable environment for lettuce growth in hydroponic cultivation with Pb solution. CONCLUSION(S): Although direct comparing the removal effect of heavy metal between BA and biochar is not present, the BA application in contaminated area suggested a significant meaning on the recycling waste, and increasing potential crop productivity by immobilizing heavy metal.

• 한국과학교육학회지
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• 제5권1호
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• pp.63-79
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• 1985

The purpose of our survey study was to investigate the status on perception and actual state toward science educational evaluation and objectives of middle school science teachers in Pusan. The perception and actual state were surveyed by the questionaire which was developed by Jong-Ok Woo et. al. We developed two kinds of questionaire. One is designed to use Likert type scale and is composed of two main category(SE; Science educational evaluation, SO; Science educational objectives) which contains 21 question items, and the other has 16 question items From 313 science teacger's responses of 75 middle schools in pusan, the tendency. of total(SE+SO) and each sub category, and the contrast with their background and actual state were analized by SPSS program of KAIST. Some results of our survey study can be summarized as follows; 1. Total tendency of science educational evaluation(SE) and objectives(SO) of middle school science teachers in pusan shows a positive and right tendency of conception in general(m-85.8/100). 2. Tendency of SE(m-87.8/100) are slightly higher than SO(m-81.7/100). At the level of 5% significance, there are correlated but shows low correlation (r=0.12(4). 3. In each correlation toward total tendency(SE+SO), SE is highly correlated(r-0.8486) but SO is moderately correlated(r=0.6297) at the level of 0.1% significance. 4. Tendencies of 5sub category (PE, PC, PD, PO, PB) shows considerably right tendencies (m=$73.4/100{\sim}92.7/100$), there are moderately correlated foward total tendency(SE+SO) at the level of 0.1% significance (r=$0.49{\sim}0.60$). 5. At the level of 5%significance, total means are no differences which their background(Sex, Final alma mate, Major, Difference of training), but there is differences to teaching career at the level of 5% significance.

• 한국산업보건학회지
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• 제11권2호
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• pp.118-125
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• 2001

Recently, biochemical analysis using hemoglobin adduct is frequently performed to evaluate the exposure to chemical carcinogens. However, data on the effect of co-exposure with other chemicals on hemoglobin adduct formation are seldom provided. The objective of this study is to evaluate the effects of pretreatment of ethanol(EtOH) and phenobarbital(PB), which are known to affect metabolism of xenobiotics, on the formation of hemoglobin adducts in the rats(Sprague-Dawley) administered benzidine(BZ). The experimental rats were divided into control, EtOH, and P8 groups. Rats were pretreated with EtOH or PB 24 hours before the oral administration of BZ. Blood sampling was taken before the administration of the chemicals and 0.5, 3, 6, 9, 12, 24, 48, 72, 96, and 144 hours after the administration of the BZ in 5 rats each. The blood was separated into hemoglobin and plasma immediately after taking the blood samples, and the adducts were undergone basic hydrolysis to convert them into aromatic amines. Hydrolyzed BZ, monoacetylbenzidine (MABZ), and 4-aminobiphenyl(4ABP) were separated by reversed-phase liquid chromatography without derivatization, and quantitative analyses of them were performed by a highperformance liquid chromatograph equipped with electrochemical detector. The quantitative amount of the metabolites was expressed by hemoglobin binding index(HBI), BZ-, MABZ-, and 4ABP-HBI of EtOH and PB groups were increased more than those of control group. These results are attributable to the fact that EtOH and PB induced N-hydroxylation related to the hemoglobin adduct formation. The ratio of N-acetylation (viz, MABZ-HBI/BZ-HBI) showed no significant difference between EtOH group and control group. It means that EtOH increased N-hydroxylation and N-acetylation in a similar degree. The N-acetylation ratio of PB group was relatively lower than control group because the PB increased N-hydroxylation induction. The N-acetylation ratios of all groups were higher than 1 during the entire experimental period. This result suggests that the effects of EtOH or PB need to be considered in the biochemical monitoring for the assessment of intermittent exposure of benzidine.

• 한국어병학회지
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• 제32권1호
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• pp.9-20
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• 2019

The study investigated the dietary effects of medicinal herbs extract and multiple probiotics mixture on the growth performance, innate immune response and antibacterial activity of nile tilapia Oreochromis niloticus. Tilapia were divided in four groups. The first is a fish group fed a basal diet added with 40% medicinal herbs extract (MHE). The second is a fish group fed a basal diet supplied with $2{\times}10^8CFU/g$ of 2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp, respectively (PB). The third group was fed with a mixture of probiotics (2 Bacillus sp, 2 Lactobacillus sp and 2 Yeast sp) with the medicinal herbs extract added in basal diet (MHE+PB). The fourth group was fed only a basal diet (C). In a non-specific immune parameters analysis, respiratory burst activity, lysozyme activity, phagocytic activity (PA), alternative complement pathway activity ($ACH_{50}$) and superoxide dismutase (SOD) activity were significantly (p<0.05) increased in the group MHE+PB compared to other groups. Both PB and MHE groups showed a significant (p<0.05) increase in respiratory burst activity, lysozyme activity compared to the control C group, whereas no significant differences were observed in PA, $ACH_{50}$ and SOD activity compared to the control group. In challenging test, fish were administered with Edwardsiella tarda (E. tarda) on 30 days after feeding with each experimental diet and viable E. tarda cell reduction was checked over 21 days post injection. MHE+PB group showed a significantly (p<0.05) reduced E. tarda cells compared to other groups. No significant antibacterial difference (p>0.05) was observed between PB and MHE only treated group. Compared to the control, a significant antibacterial difference (p<0.05) appeared in PB but not in MHE (p>0.05). The results suggest that the probiotics and MHE mixture could be utilized as an alternative to antibiotics in the control of fish diseases caused by E. tarda.