• 환경위생공학
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• 제21권2호
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• pp.1-10
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• 2006

Codonopsis pilosula and Adenophora triphylla were cultivated by sawing seeds, but the germination rate were low. To cultivate these two wild plants was very difficult. The marketable roots of the plants had low quality. The plant pathogens were very difficulty to prevent the diseases (Rhizoctonia, Pythium, Fusarium, Erwinia, Botrytis, Phythophthora) appeared in cultural state. For the extermination of the disease needs a lot of agricultural chemicals, the effect of remain behind an insecticides was high of a hazardous rate after harvest. On this studies, for the safe prevention of the diseases and the promote of seeds germination, we used Bacillus subtilis, B. liquefaciens, Paenibacillus polymixa, Pseudomonas putida separated in our research, these results would bring us harmless products of medicinal herbs for human.

• 지역사회간호학회지
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• 제5권2호
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• pp.130-146
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• 1994

Our medical care system is trying to diversify in order to meet the client's needs, and to adjust to a medical environment which is changing very rapidly. Because current nursing theory and practice focus on holistic care, health care management, education, and research, contrary to the traditional emphasis on only assisting a physician, more autonomy and specialization for the implementation of nursing are required. Considering these trends and actual needs, the category of clinical nurse specialist should be established as soon as possible. In order to develop strategies for implementing this new professional specialty, the authors conducted a field survey and literature review of the current system in Korea. As a result, various obstacles and constraints were discovered as follows : 1) There are few accredited educational programs for the training of CNS's. 2) Several hospitals already have staff designated as clinical nurse specialist (CNS) even though the term CNS is not yet standardized or adopted in nationwide. 3) The role of the CNS is not clearly understood by the medical societies, or even nursing societies. A nurse who works in specific nursing areas such as central supply, kidney dialysis, intensive care, coronary care, etc. for a long time, considers herself /himself a CNS. Based upon the above findings, the following alternatives are recommended. 1) The role of the CNS should be defined according to specified functions and authority : professional autonomy ; counselling and educating patients and their familes, nurses, and even other medical personnel ; research on improvement of nursing ; and management of the nursing environment including medical resources, information, and cases. 2) the qualification of CNS should be attained only by a nurse who has an RN license and clinical experience of more than 3 years in a specific nursing field: passes a qualifying examination; and contributes to the professional development of peers, colleagues, and others. A master's degree should only be optional, because of the insufficient of graduate programs which are well designed for the CNS. 3) The CNS should initially be a head nurse rather than line staff in order to deal with as wide an experience base as possible. 4) The nursing specialty could be divided into two areas such as a clinical field and a community field. The clinical field could then be categorized by the Styles' classification such as diseases and pathogenics, systems, ages, acuity, skills/techniques, and function/role ; the community field could be classified according to work site.

• Journal of Animal Science and Technology
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• 제48권5호
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• pp.709-718
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• 2006

식물유래 생리활성물질인 garlic, scallion, fla- vonoid, urushiol 및 anthocyanidin과 기존의 항균 제품인 Bio-MOS?瑛? 병원성 미생물과 반추위 미생물에 대한 효과를 조사하기 위하여 병원성 균인 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes, Staphylococcus aureus에 항균활성과 in vitro 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물 수를 측정하였다. 생리활성물질 0.10% 첨가 후, 배양시험에서는 flavonoid, scallion 및 Bio-MOS?怜? 병원성 미생물의 성장을 억제하였으나, paper disc법에 의한 항균활성 시험에서는 flavonoid나 scallion을 5.00%이상 처리 시 clear zone이 뚜렷하였으며, scallion이 가장 강력한 항균활성을 가지고 있는 것으로 나타났다. 생리활성물질의 in vitro 반추위 미생물 발효에 대한 영향은 크지 않았는데, 병원균에 활성을 나타내었던 flavonoid는 1.00% 처리 시 CMCase의 활성이 완전히 없어져 반추위 미생물에 대한 억제효과가 있는 것으로 나타났다. 그러나 scallion은 병원균에는 강한 항균활성을 가지면서 반추위 미생물 발효에는 저해작용을 나타내지 않아 반추 가축의 대체 천연항균제로서 개발 가능성을 나타내었다.

• 생명과학회지
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• 제17권11호
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• pp.1555-1561
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• 2007

본 연구는 무기물성 및 식물성 유래 생리활성물질로서 scoria, germanium, charcoal, 생강, stevia 및 CLA(conjugated linoleic acid)가 병원성 미생물 및 반추위 미생물에 대한 작용을 조사하기 위하여 수행되었다. 병원성 균으로서 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes 및 Staphylococcus aureus에 적용하여 항균활성을 측정하고, in vitro 발효 시 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물의 수를 측정하였다. 병원성 미생물 배양액에 생강을 0.1% 첨가한 구에서만 항균활성이 나타났으나, paper disc법에 의한 항균활성 시험에서는 stevia 10%첨가구와 CLA 10% 첨가구에서 E. coli에 대해서 항생제 첨가구인 positive control 구와 비슷한 크기의 clear zone을 형성하였다. in vitro 반추위 미생물 발효시험에서는 생강, stevia 및 CLA가 반추위 박테리아와 프로토조아의 증식을 억제하는 것으로 나타났는데, 특히 생강 첨가구의 경우 메탄 생성균의 서식지로 알려져 있는 프로토조아를 크게 억제함으로써 메탄생성 억제제로서 개발 가능성이 있는 것으로 사료된다.