• Korean Chemical Engineering Research
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• v.55 no.4
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• pp.510-513
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• 2017

Patchouli oil extraction in general is still using conventional methods that require a long time of extraction. It is therefore necessary to develop extraction methods to obtain patchouli oil with optimum yield and quality. One of the new methods, which has been successfully developed, is microwave hydrodistillation (MHD). In addition to optimizing the extraction process of patchouli oil, this study also used microwave air-hydrodistillation (MAHD). Based on the research results, extraction using MAHD method can produce higher yield of patchouli oil when compared using MHD method. Also, based on the results of the analysis by GC-MS, extraction using MAHD method can produce quality of patchouli oil that is almost the same when compared using MHD method. This is supported by the results of the analysis by GC-MS, which showed that the content of patchouli alcohol is the main component of patchouli oil, and is almost the same for patchouli oil extracted using MHD method (26.32%) and MAHD method (25.23%).

• Journal of the Korean Society of Tobacco Science
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• v.24 no.2
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• pp.101-106
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• 2002

This study was conducted to investigate the pyrolysis products of patchouli oil by Curie-Point pyrolysis. The pyrolysis of patchouli oil was performed at the temperature of 16$0^{\circ}C$, 42$0^{\circ}C$, $650^{\circ}C$, 76$0^{\circ}C$, and 92$0^{\circ}C$ by Curie-Point Pyrolyzer. The pyrolysis products were analyzed by gas chromatography(GC) and mass selective detector(MSD). Total 21 components were identified in the pyrolyzates of patchouli oil. The temperature for maximum formation of most of these compounds was in the range of 76$0^{\circ}C$~92$0^{\circ}C$. The major components were $\beta$-patchoulene, $\alpha$-guaiene, $\beta$-caryophyllene, $\alpha$-patchoulene, seychellene, $\delta$-guaiene, and patchouli alcohol. The numbers of the pyrolyzed products of patchouli oil were increased by increasing temperature, however, the yields of major components such as patchoulene, guaiene, seychellene and patchouli alcohol decreased as the temperature of pyrolysis was raised to 92$0^{\circ}C$, the highest temperature in this experiment. The optimum temperature for formation of the pyrolysis products such as styrene, indane and naphthalene was at 92$0^{\circ}C$.

• Natural Product Sciences
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• v.27 no.3
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• pp.208-215
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• 2021

𝛽-Patchoulene (𝛽-PAE) is a tricyclic sesquiterpene which performed many potential bioactivities and can be found in patchouli oil but in very low concentration. This study aimed to obtained 𝛽-PAE in high concentration by conversion of patchouli alcohol (PA) in patchouli oil under acid catalyzed reaction. Patchouli oil was fractinated by vacuum distillation at 96 kPa to get the fraction with the highest PA content. H₂SO₄ and ZnCl₂ were used respectively as homogeneous and heterogeneous acid catalysts in the conversion reaction of the selected fraction. Patchouli oil, the fractions and the products were analysed by using GC-MS and FTIR instruments. Moreover, the interaction of 𝛽-PAE to COX-2 protein was studied to understand the antiinflammation activity of 𝛽-PAE. The results showed that patchouli oil contains 25.3% of PA. The selected fraction which has the highest PA content (70.3%) was distilled at 151 - 152 ℃. The application of ZnCl₂ catalyst in conversion reaction did not succeed. In contrast, H₂SO₄ as a catalyst in acetic acid solvent succeeded in converting the overall fraction of PA to 𝛽-PAE. Furthermore, the molecular docking study of 𝛽-PAE against COX-2 enzyme showed van der Waals and alkyl-alkyl stacking interactions on ten amino acid residues.

• Korean Journal of Pharmacognosy
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• v.29 no.1
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• pp.18-21
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• 1998

A method for isolation and quantitative determination of patchouli alcohol from Pogostemonis Herba (Pogostemon cablin) has been developed. Isolation of patchouli alcohol was achieved by column chromatography employing the normal-phase gradient separation system n-hexane-ether on silica gel column. The GC method for quantitative determination of patchouli alcohol provided a method for standardization of the raw drug. It suggested that the average content of patchouli alcohol in Pogostemonis Herba is about 0.26%.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.1
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• pp.33-39
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• 2009

To develop a new natural whitening agent for cosmetics, we investigated the inhibitory effects of Pogostemon cablin Bentham extracts (PCE) and its active component on melanogenesis. PCE showed ROS scavenging activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and xanthine/xanthine oxidase system with the $IC_{50}$ values of $24.2{\pm}2.85{\mu}g/mL$ and $IC_{50}=118{\pm}0.43{\mu}g/mL$, respectively. PCE reduced melanin contents of B16 melanoma cells in a dose-dependant manner and decreased to about 23 % at a concentration of $20{\mu}g/mL$ without cell cytotoxicity (below $100{\mu}g/mL$). And the PCE reduced intracellular tyrosinase activity about 18 % at concentration of $50{\mu}g/mL$. We purified one active compound from PCE and identified its structure. It was identified as patchouli alcohol, sesquiterpene family, by 1H-NMR, $13_C$-NMR, and Mass analysis. Patchouli alcohol also inhibited ROS scavenging activities in DPPH radical and xanthine/xanthine oxidase system with the $IC_{50}$ values of $3.14{\pm}0.12{\mu}g/mL$ and $49{\pm}3.24{\mu}g/mL$, respectively. Patchouli alcohol inhibited melanin synthesis in a dose dependent manner ($IC_{50}=3.9{\mu}g/mL$). And the patchouli alcohol reduced intracellular tyrosinase activity about 40 % at concentration of $10{\mu}g/mL$. Patchouli alcohol inhibited tyrosinase and TRP-2 expression at protein level. These results suggest that PCE and patchouli alcohol reduced melanin formation by the inhibited of tyrosinase activity and expression in B16 melanoma cells. Therefore, we suggest that PCE could be used as a useful whitening agent.

• Archives of Pharmacal Research
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• v.26 no.5
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• pp.389-393
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• 2003

The essential oils from Cedrus atlantica, Styrax tonkinensis, Juniperus communis, Lavandula angustifolia, Melaleuca alternifolia, Pelargonium graveolens, Pogestemon patchouli and Rosmarinus officinalis were analyzed by GC-MS. Antifungal activities of the oils were investigated by disk diffusion assay and the broth dilution method against Aspergillus niger and A. flavus. The effects of geraniol and the essential oil fraction from P. graveolens on the antifungal activity of amphotericin Band ketoconazole were examined using a checkerboard microtiter assay against both Aspergillus fungi. Most of the tested essential oils, with the exception of C. atlantica, J. communis, and P. patchouli, significantly inhibited growth of A. niger and to a lesser extent that of A. fIavus, with MICs (minimal inhibitory concentrations) in the range 0.78-12.5 mg/mL. The essential oil fraction of P. graveolens and its main components, geraniol and citronellol, exhibited additive effects with amphotericin B and with ketoconazole against both Aspergillus species, resulting in fractional inhibitory concentration (FIC) indices ranging from 0.52 to 1.00.

• The Journal of Industrial Distribution & Business
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• v.10 no.12
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• pp.15-23
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• 2019

Purpose : We will develop the distribution method of wide oil extract technology to develop repellent using this technology and study mosquito repellent persistence. Research design, data and methodology : A positive control group containing purified water, ethanol and picaridin was prepared, and the experimental control group was prepared in the same proportion as the positive control group, and 0.6% of broad oil was added. The results were summarized using the calculation method according to the avoidance effect and statistically tested by t-test using the excel statistics program. Results : Experiments on skin surface area and voice control of participants showed that men had 8.9% wider skin surface area than women, and voice control tests showed that women were bitten by mosquitoes five times more than men. Both the positive and the experimental control groups had a valid duration of up to three hours, but from the time of five hours, the positive control group had 77% and the experimental control had 90%, indicating a difference of 14.4% over the positive control group. Conclusions : The mosquito repellents developed in this study on the basis of safety and continuity are cost-effective in terms of mosquito repellent, and in addition fragrance, odor removal, perfume, ink, skin care and massage effect.

• Journal of the Korean Society of Fashion and Beauty
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• v.3 no.3 s.3
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• pp.31-40
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• 2005

The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

• Journal of Korean Academy of Nursing
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• v.36 no.2
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• pp.236-243
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• 2006

Purposes: This experimental study was designed to verify the effect of inhalation of essential oils on body weight, feed intake, food efficiency rate and serum leptin. Methods: The subjects of this study were 90 growing SD rats(46 males and 44 females). They were allocated into one of four groups, the Fennel group, Patchouli group, Bergamot group and control group. The experimental treatment was the inhalation of aromatherapy essential oils which was applied two times a day for 10 minutes each during 8 weeks. To evaluate the effects, body weight, feed intake, food efficiency rate and serum leptin were measured before and after the treatment. The collected data was analyzed by repeated measures of Kolmogorov-smirnov test and Normal Q-Q plot for nomality, Kruskal Wallis test and $X^2-test$ for experimental effects with the SPSS program. Results: The food efficiency rate was significantly lower in the Patchouli group and Fennel group than in the Bergamot group and control group(P=.000). No significant group effects were found for SD rat's body weight, feeding amount and serum leptin. Conclusion: In conclusion, these findings indicate that the inhalation of essential oils could be effective in lowering the food efficiency rate rather than the feed intake.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.45-49
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• 2006

Fumigant toxicity of four plant essential oils (muguet flower, patchouli, pennyroyal, spearmint) were tested against the adults of western flower thrips, Frankliniella occidentalis. Among them, pennyroyal and spearmint oils showed 100% mortality at $1{\mu}{\ell}/45m{\ell}$ air dose. Through the constituent analysis using GC and GC-MS, we confirmed that main constituents of pennyroyal oil were pulegone(100.0%), and spearmint oil were $\beta-myrcene(3.5%)$, limonene(12.1%), carvone(85.4%). Among them, carvone and pulegone showed 100% fumigation at $1{\mu}{\ell}/45m{\ell}$ air dose, respectively. It can concluded that two oils are potential control agents against F. occidentalis.