• Korean Journal of Computational Design and Engineering
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• v.5 no.2
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• pp.196-205
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• 2000

This paper proposes a topology-based algorithm for recognizing the passage features using a concept of multi-face hole loop. The Multi-face hole loop is a concetpual hole loop that is formed over several connected faces. A passage feature is recognized in the proposed approach by two multi-face hole loops that constitute its enterance and exit. The algorithm proposed in this paper checks the connectivity of the two multi-face hole loops to recognize passage features. The total number of passage features in a part is calculated from Euler equation and is compared with the number of found passage features to decide when to terminate. To find all multi-face hole loops in a part, this paper proposes an algorithm for finding all combinations of connected faces. The edge convexity is used to judge the validity of multi-face hole loops. By using the algorithm proposed in this paper, the passage features could be recognized effectively. The approach proposed in this paper is illustrated with several example parts.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.1
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• pp.44-47
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• 2000

Cell growth and DNA synthesis were studied from a cultured early- and late- pas- sage mouse aorta smooth muscle cell (MASMC) because the proliferation of vascular smooth muscle cell (VSMC) is a key factor in development of atherosclerosis. In this study, the cells were cultured in fetal bovine serum (FBS) and stimulated by growth factors such as thrombin and platelet-derived growth factor-BB (PDGF-BB). Compared to the number of early-passage MASMC (passage 3 to 9) the number of late-passage MASMC (passage 30 to 40) in a normal serum state was increased 2 fold at Day 1, 3 and 6 in culture, respectively. Incorporation of $[^3H]$ thymidine into DNA induced by serum, PDGF and thrombin in late-passage MASMC was greater than those in early-passage MASMC. We also examined whether intracellular zinc levels would be an aging factor or not. The intracellular zinc level in early- and late-passage MASMC was monitored by using the zinc probe dye N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide. It is interested that late-passage MASMC increased the intracellular fluorescence level of zinc, more than the early passage MASMC did. The alterations of intracellular zinc level occur concurrently with changes in MASMC proliferation rate during aging. This data suggest that the age-associated changes in zinc concentrations may provide a new in vitro model for the study of smooth muscle cell differentiation.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1800-1807
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• 2016

To understand how human cytomegalovirus (HCMV) might change and evolve after reactivation, it is very important to understand how the nucleotide sequence of cultured HCMV changes after in vitro passaging in cell culture, and how these changes affect the genome of HCMV and the consequent variation in amino acid sequence. Strain JHC of HCMV was propagated in vitro for more than 40 passages and its biological and genetic changes were monitored. For each passage, real-time PCR was performed in order to determine the genome copy number, and a plaque assay was employed to get virus infection titers. The infectious virus titers gradually increased with passaging in cell culture, whereas the number of virus genome copies remained relatively unchanged. A linear correlation was observed between the passage number and the log₁₀ infectious virus titer per virus genome copy number. To understand the genetic basis underlying the increase in HCMV infectivity with increasing passage, the whole-genome DNA sequence of the high-passage strain was determined and compared with the genome sequence of the low-passage strain. Out of 100 mutations found in the high-passage strain, only two were located in an open reading frame. A G-T substitution in the RL13 gene resulted in a nonsense mutation and caused an early stop. A G-A substitution in the UL122 gene generated an S-F nonsynonymous mutation. The mutations in the RL13 and UL122 genes might be related to the increase in virus infectivity, although the role of the mutations found in noncoding regions could not be excluded.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1506-1511
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• 2003

The present study proposes a new structure for a flat plate heat pipe which could embody a thin thickness, any shapes and high heat density a unit area. It is on the structure for the formation of vapor passages and the support of the case of the flat plate heat pipe. A screen mesh is used as the one. To verify the validity of the one, the flat plate heat pipe of 1.08mm thickness was made with a layer of the screen mesh with 14 and 100 mesh number respectively and tested. Here the screen mesh with 14 mesh number plays a role of the vapor passage and the support of the case and the screen mesh with 100 mesh number functions as the wick structure. T he results show that the screen mesh excellently carries out the function of the vapor passage and the support of the case.

• Journal of the Korean Society of Visualization
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• v.18 no.2
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• pp.35-38
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• 2020

Airfryer is used to heat a food up by convecting hot air upward around the food. In this study, we investigated the effect of the bottom-shape of the food container in airfryer on the upward convection velocity of hot air to find an optimal bottom-shape by computational fluid dynamics. Numerical experiments were performed by solving the incompressible Navier-Stokes equations with turbulence model. We found that the maximum upward velocity with concave flow-passage on the bottom was bigger than that with the flat bottom and that the maximum upward convection velocity was achieved when the number of concave flow-passage with fan-shape is around six. The pressure drop by the internal flow was found to increase as the number of the concave flow-passage on the bottom increased probably due to increase of the surface area of the bottom. Therefore, it can be said that the optimal number of the concave flow-passage is around six for the flow rate considered in this study.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.3
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• pp.350-357
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• 2017

The purpose of this study was to evaluate the difference of differentiation potential in each passage of dental pulp stem cells from supernumerary tooth (sDPSCs). The sDPSCs were obtained from a healthy 6-year-old male patient under the guidelines and got the informed consent. Cells were cultured until passage number 16 and divided into two groups; 1 - 8 passages as a young group and 9 - 16 passages as an old group. It was taken $2.25{\pm}0.46days$ in a young group and $3.25{\pm}0.46days$ in an old group to propagate cells of each passage until confluence and there were statistically significant differences between two groups (p < 0.05). In every passage, cell morphology was observed with microscope and evaluated the capacity to form high levels of minerals by alizarin red solution staining after treating differentiation medium. Fibroblast-like, spindle shaped, elongated cells and a few nodules were found in uninduced cultures of passage number 1, 8 and 9. But at 16 passage culture, cell size became larger and broader and observed with more nodules. After inducing differentiation, mineralized nodules were detected at the first passage of 7th day culture whereas at the 8 passage culture, nodules were seen clearly at 14th day culture. In addition, the amount of mineralized nodules were remarkably decreased after passage 9. From the data presented in this study, it is recommended to use sDPSCs of passage number within 8 for utilizing as stem cells.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.33 no.6
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• pp.427-434
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• 2009

A new surface shape of an internal cooling passage which largely reduces the pressure drop and enhances the surface heat transfer is proposed in the present study. The surface shape of the cooling passage is consisted of the concave dimple and the riblet inside the dimple which is protruded along the stream-wise direction. Direct Numerical Simulation (DNS) for the fully developed turbulent flow and thermal fields in the cooling passage is conducted. The numerical simulations for five different surface shapes are conducted at the Reynolds number of 2800 based on the mean bulk velocity and channel height and Prandtl number of 0.71. The driving pressure gradient is adjusted to keep a constant mass flow rate in the x direction. The thermoaerodynamic performance for five different cases used in the present study was assessed in terms of the drag, Nusselt number, Fanning friction factor, volume and area goodness factor in the cooling passage. The value of maximum ratio of drag reduction is -22.86 %, and the value of maximum ratio of Nusselt number augmentation is 7.05% when the riblet angle is $60^{\circ}$. The remarkable point is that the ratio of Nusselt number augmentation has the positive value for the surface shapes which have over $45^{\circ}$ of the riblet angle. The maximum volume and area goodness factors are obtained when the riblet angle is $60^{\circ}$.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2465-2470
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• 2008

A new surface shape of an internal cooling passage which largely reduces the pressure drop and enhances the surface heat transfer is proposed in the present study. The surface shape of the cooling passage is consisted of the concave dimple and the riblet inside the dimple which is protruded along the stream-wise direction. Direct Numerical Simulation (DNS) for the fully developed turbulent flow and thermal fields in the cooling passage is conducted. The Numerical simulations for the 5 different surface shapes are conducted at the Reynolds number of 2800 based on the mean bulk velocity and channel height and Prandtl number of 0.71. The driving pressure gradient is adjusted to keep a constant mass flow rate in the x direction. The thermo-aerodynamic performance for the 5 different cases used in the present study was assessed in terms of the drag, Nusselt number, Fanning friction factor, Volume and Area goodness factor in the cooling passage. The value of maximum ratio of drag reduction is -22.86 [%], and the value of maximum ratio of Nusselt number augmentation is 7.05 [%] when the riblet angle is $60^{\circ}$ (Case5). The remarkable point is that the ratio of Nusselt number augmentation has the positive value for the surface shapes which have over $45^{\circ}$ of the riblet angle. The maximum Volume and Area goodness factor are obtained when the riblet angle is $60^{\circ}$ (Case5).

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.588-595
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• 2013

Mesenchymal stem cells (MSCs) are often known to have a therapeutic potential in the cell-mediated repair for fatal or incurable diseases. In this study, canine umbilical cord MSCs (cUC-MSCs) were isolated from umbilical cord matrix (n = 3) and subjected to proliferative culture for 5 consecutive passages. The cells at each passage were characterized for multipotent MSC properties such as proliferation kinetics, expression patterns of MSC surface markers and self-renewal associated markers, and chondrogenic differentiation. In results, the proliferation of the cells as determined by the cumulative population doubling level was observed at its peak on passage 3 and stopped after passage 5, whereas cell doubling time dramatically increased after passage 4. Expression of MSC surface markers (CD44, CD54, CD61, CD80, CD90 and Flk-1), molecule (HMGA2) and pluripotent markers (sox2, nanog) associated with self-renewal was negatively correlated with the number of passages. However, MSC surface marker (CD105) and pluripotent marker (Oct3/4) decreased with increasing the number of subpassage. cUC-MSCs at passage 1 to 5 underwent chondrogenesis under specific culture conditions, but percentage of chondrogenic differentiation decreased with increasing the number of subpassage. Collectively, the present study suggested that sequential subpassage could affect multipotent properties of cUC-MSCs and needs to be addressed before clinical applications.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.29 no.6 s.237
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• pp.737-746
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• 2005

The present study investigates heat/mass transfer and flow characteristics in a ribbed rotating passage with turning region. The duct has an aspect ratio (W/H) of 0.5 and a hydraulic diameter ($D_h$) of 26.67 mm. Rib turbulators are attached in the cross arrangement on the leading and trailing surfaces of the passage. The ribs have a rectangular cross section of $2\;mm\;(e){\times}\;mm\;(w)$ and an attack angle of $70^{\circ}$. The pitch-to-rib height ratio (p/e) is 7.5, and the rib height-to-hydraulic diameter ratio ($e/D_h$) is 0.075. The rotation number ranges from 0.0 to 0.20 while the Reynolds number is constant at 10,000. To verify the heat/mass transfer augmentation, internal flow structures are calculated for the same conditions using a commercial code FLUENT 6.1. The heat transfer data of the smooth duct for various Ro numbers agree well with not only the McAdams correlation but also the previous studies. The cross-rib turbulators significantly enhance heat/mass transfer in the passage by disturbing the main flow near the surfaces and generating one asymmetric cell of secondary flow skewing along the ribs. Because the secondary flow is induced in the first-pass and turning region, heat/mass transfer discrepancy is observed in the second-pass even for the stationary case. When the passage rotates, heat/mass transfer and flow phenomena change. Especially, the effect of rotation is more dominant than the effect of the ribs at the higher rotation number in the upstream of the second-pass.