• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.204-204
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• 2022

Transformant construction using protoplasts requires less sample preparation time than particle bombardment and Agrobacterium-mediated transfection. There are two protoplast transfection methods: the PEG-mediated transfection method and the Lipofectamine transfection method. When Lipofectamine is mixed with DNA, Lipofectamine surrounds DNA like a cell membrane because of the positive charge of Lipofectamine. The Lipofectamine-DNA complex makes DNA insertion into cells easier. Fectin has similar functions to lipofectamine and is less expensive than lipofectamine. The 3D-fectin technology has been highlighted in animal cell transfection. Therefore, we performed PEG-mediated transfection, Lipofectamine transfection, and 3D-pectin transfection with a GFP construct. Protoplasts were isolated using the first leaf of "Bobwhite" after 4 hours of incubation in an isolation Buffer (cellulase + macerozyme). Protoplasts transformed by each method were cultured for 48 hours, and then GFP fluorescence expression was confirmed under confocal microscopy. GFP signals were detected in PEG-mediated transfection and Lipofectamine transfection. And the GFP signals were also detected in protoplasts to which 3D-fectin technology was applied, suggesting that 3D-fectin technology can be used for plant protoplast transfection.

• Journal of the Korean Society of Radiology
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• v.3 no.1
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• pp.11-15
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• 2009

In this study, the basic research verifying possibility of applications as radiology image sensor in Digital Radiography was performed, the radiology image sensor was fabricated using a multi-layer technique to decrease dark current. High efficiency materials in substitution for Amorphous Selenium(a-Se) have been studied as a direct method of imaging detector in Digital Radiography to decrease dark current by using PN junction or Hetero junction already used as solar cell, semiconductor. Particle-In -Binder method is used to fabricate radiology image sensor because it has a lot of advantages such as fabrication convenient, high yield, suitability for large area sensor. But high leakage current is one of main problem in Particle-In -Binder method. To make up for the weak points, multi-layer technique is used, and it is considered that high efficient digital radiation sensor can be fabricated with easy and convenient process. In this study, electrical properties such as leakage current, sensitivity, signal linearity is measured to evaluate multi-layer radiation sensor material.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.12
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• pp.2436-2440
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• 2009

In this work, TCO-less dye-sensitized solar cells (DSCs) using Ti-mesh layer is fabricated for high-efficient low-cost solar cell application. The Ti-mesh metal can replace TCO in the photo-electrode part of DSCs, thus the cell structure is composed of a glass/dye sensitized TiO2 particle/ Ti-mesh layer/electrolyte/Pt sputtered counter electrode/ glass. The Ti-mesh electrode with high conductivity can collect electrons from the $TiO_2$ layer and allows the ionic diffusion of $I^-/I_3^-$ through the mesh hole. Thin Ti-mesh ($\sim40{\mu}m$ in thickness) electrode material is processed using rapid prototype method. The efficiency of prepared TCO-less DSCs sample is about 1.45 % ((ff: 0.5, Voc: 0.52V, Jsc: 5.55 $mA/cm^2$).

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.852-855
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• 2010

Numerical simulations for large scale triaxial tests with large diameter rockfill materials are conducted using distinct element method. For generation of compacted assembly with specific grain size distribution and initial material porosity, the clump logic method and expansion of generated particles are adapted. With micro parameters which are chosen by calibration process, discrete particle modelling of triaxial test in case of other confining stress and cyclic loading condition were conducted. Also numerical simulations of fluid injection into particulate materials were conducted to observe cavity initiation and propagation using distinct element method. The fluid scheme solves the continuity and Navior-Stokes equations numerically, then derives pressure and velocity vectors for fixed grid by considering the existence of particles within the fluid cell.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.05a
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• pp.236-237
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• 2017

This study discusses the development of waterproofing layer jet-spray nozzle that forms a three-dimensional air cell. This nozzle has an air flow generation mechanism in the air groove of the attachment cell part located at the end of the injection nozzle. Since the air grooves also function as an air curtain, the airborne particles generated when the waterproof material is sprayed is effectively blocked. In the past, spraying of the waterproof material through the high pressure was possible, but this technology allows stable injection due to the static agitation method, and various problems caused by particle generation has been (damages to neighboring areas, economic loss, etc.) minimized.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.2
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• pp.187-194
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• 2012

The strength of particle-reinforced metal matrix composites is, in general, known to be increased by the geometrically necessary dislocations punched around a particle that form during cooling after consolidation because of coefficient of thermal expansion (CTE) mismatch between the particle and the matrix. An additional strength increase may also be observed, since another type of geometrically necessary dislocation can be formed during extensive deformation as a result of the strain gradient plasticity due to the elastic-plastic mismatch between the particle and the matrix. In this paper, the magnitudes of these two types of dislocations are calculated based on the dislocation plasticity. The dislocations are then converted to the respective strengths and allocated hierarchically to the matrix around the particle in the axisymmetric finite-element unit cell model. The proposed method is shown to be very effective by performing finite-element strength analysis of $SiC_p$/Al2124-T4 composites that included ductile failure in the matrix and particlematrix decohesion. The predicted results for different particle sizes and volume fractions show that the length scale effect of the particle size obviously affects the strength and failure behavior of the particle-reinforced metal matrix composites.

• Korean Journal of Materials Research
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• v.29 no.5
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• pp.282-287
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• 2019

The photovoltaic properties of $TiO_2$ used for the electron transport layer in perovskite solar cells(PSCs) are compared according to the particle size. The PSCs are fabricated and prepared by employing 20 nm and 30 nm $TiO_2$ as well as a 1:1 mixture of these particles. To analyze the microstructure and pores of each $TiO_2$ layer, a field emission scanning electron microscope and the Brunauer-Emmett-Teller(BET) method are used. The absorbance and photovoltaic characteristic of the PSC device are examined over time using ultraviolet-visible-near-infrared spectroscopy and a solar simulator. The microstructural analysis shows that the $TiO_2$ shape and layer thicknesses are all similar, and the BET analysis results demonstrate that the size of $TiO_2$ and in surface pore size is very small. The results of the photovoltaic characterization show that the mean absorbance is similar, in a range of about 400-800 nm. However, the device employing 30 nm $TiO_2$ demonstrates the highest energy conversion efficiency(ECE) of 15.07 %. Furthermore, it is determined that all the ECEs decrease over time for the devices employing the respective types of $TiO_2$. Such differences in ECE based on particle size are due to differences in fill factor, which changes because of changes in interfacial resistance during electron movement owing to differences in the $TiO_2$ particle size, which is explained by a one-dimensional model of the electron path through various $TiO_2$ particles.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.1
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• pp.56.2-56.2
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• 2018

Astrophysical shocks accelerate particles to high velocities, which we observe as cosmic rays. The acceleration process changes the nature of the shock because the particles interact with the local magnetic field, removing energy and potentially triggering instabilities. In order to simulate this process, we need a computational method that can handle large scale structures while, at the same time, following the motion of individual particles. We achieve this by combining the grid magnetohydrodynamics (MHD) method with the particle-in-cell (PIC) approach. MHD can be used to simulate the thermal gas that forms the majority of the gas near the shock, while the PIC method allows us to model the interactions between the magnetic field and those particles that deviate from thermal equilibrium. Using this code, we simulate shocks at various sonic and Alfvenic Mach numbers in order to determine how the behaviour of the shock and the particles depends on local conditions.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.10 no.3
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• pp.185-219
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• 1981

To analyze two dimensional incompressible laminar natural convection in a rectangular enclosure heated from below and cooled by a horizontal ceiling and two vertical walls, he primitive Navier-Stokes equations and the energy equation were solved numerically in time dependent form by a marker and cell method. A successive over-relaxation method for the elliptic portion of the problem and an explicit method for the parabolic portion were applied for the range of Grashoff number of $5{\times}10^3\;to\;5{\times}10^4$ to get the transient and steady state dimensionless temperature and velocity profiles. For the range of aspect ratio $L/H{\leqq}2.4$ in which only a pair of convection rolls exists mean Nusselt number calculated are as follows : $$N_{NU}0.89\;N_{Gr}^{0.2}(H/L)^{0.45}$$ By path lines drawn by marker particle trajectories roll number of cellular motion were observed for various aspect ratio of the enclosure.

• Journal of Powder Materials
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• v.27 no.1
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• pp.63-71
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• 2020

It is necessary to fabricate uniformly dispersed nanoscale catalyst materials with high activity and long-term stability for polymer electrolyte membrane fuel cells with excellent electrochemical characteristics of the oxygen reduction reaction and hydrogen oxidation reaction. Platinum is known as the best noble metal catalyst for polymer electrolyte membrane fuel cells because of its excellent catalytic activity. However, given that Pt is expensive, considerable efforts have been made to reduce the amount of Pt loading for both anode and cathode catalysts. Meanwhile, the atomic layer deposition (ALD) method shows excellent uniformity and precise particle size controllability over the three-dimensional structure. The research progress on noble metal ALD, such as Pt, Ru, Pd, and various metal alloys, is presented in this review. ALD technology enables the development of polymer electrolyte membrane fuel cells with excellent reactivity and durability.