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Physiological Activity of Robinia pseudo acacia Leaf Extracts and Enhancement of Skin Permeation Using Polymer Micelles and Cell Penetrating Peptide (아카시아 잎 추출물의 생리 활성 및 고분자 미셀과 세포투과 펩티드를 적용한 피부흡수증진 효과)

  • Heo, Soo Hyeon;Park, Su In;An, Gyu Min;Shin, Moon Sam
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.3
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    • pp.271-282
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    • 2019
  • This study was conducted to evaluate physiological activity of Robinia pseudo-acacia leaf and its skin penetration using polymer micelles and skin penetrating peptide. After extraction with Robinia pseudo-acacia using the ethanol and distilled water, various physiological activities were examined. The total concentration of polyphenol compounds was determined to be 47.42 mg/g (ethanol extract), 56.88 mg/g (hydrothermal extract) and DPPH radical scavenging ability at $1,000{\mu}g/mL$ was 44.24% in ethanol extract and it is higher than value(41.50%) in hydrothermal extract. The elastase inhibitory assay showed concentration dependence and elastase inhibition of Robinia pseudo acacia leaf ethanol extract was 54.09%, which was the highest at $500{\mu}g/mL$. In the SOD-like experiments, the concentration-dependent results were showed and the SOD-like activity of the Robinia pseudo-acacia leaf ethanol extract was higher than that of the Robinia pseudo acacia leaf hydrothermal extract at all concentrations. At a concentration of $500{\mu}g/mL$, Robinia pseudo acacia leaf ethanol extract showed the highest SOD-like activity of 76.41%. The tyrosinase inhibition at $20{\mu}g/mL$ was determined to be 56.47% (ethanol extract), 23.05% (hydrothermal extract). In the antimicrobial experiments, the hydrothermal extract had no effect, but ethanol extract represented maximum clear zone of 11.00 mm in Propionbacterium acnes strain and maximum clear zone of 10.50 mm. in Bacillus subtilis strain. To solve the problem of insolubility and to improve skin penetration, PCL-PEG polymer micelles containing Robinia pseudo-acacia leaf ethanol extracts and 1.0% cell permeable peptide, hexa-D-arginine (R6) were successfully prepared with particle size of 108.23 and 126.47 nm and excellent skin permeation effects could be showed.

A Comparison between Multiple Satellite AOD Products Using AERONET Sun Photometer Observations in South Korea: Case Study of MODIS,VIIRS, Himawari-8, and Sentinel-3 (우리나라에서 AERONET 태양광도계 자료를 이용한 다종위성 AOD 산출물 비교평가: MODIS, VIIRS, Himawari-8, Sentinel-3의 사례연구)

  • Kim, Seoyeon;Jeong, Yemin;Youn, Youjeong;Cho, Subin;Kang, Jonggu;Kim, Geunah;Lee, Yangwon
    • Korean Journal of Remote Sensing
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    • v.37 no.3
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    • pp.543-557
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    • 2021
  • Because aerosols have different spectral characteristics according to the size and composition of the particle and to the satellite sensors, a comparative analysis of aerosol products from various satellite sensors is required. In South Korea, however, a comprehensive study for the comparison of various official satellite AOD (Aerosol Optical Depth) products for a long period is not easily found. In this paper, we aimed to assess the performance of the AOD products from MODIS (Moderate Resolution Imaging Spectroradiometer), VIIRS (Visible Infrared Imaging Radiometer Suite), Himawari-8, and Sentinel-3 by referring to the AERONET (Aerosol Robotic Network) sun photometer observations for the period between January 2015 and December 2019. Seasonal and geographical characteristics of the accuracy of satellite AOD were also analyzed. The MODIS products, which were accumulated for a long time and optimized by the new MAIAC (Multiangle Implementation of Atmospheric Correction) algorithm, showed the best accuracy (CC=0.836) and were followed by the products from VIIRS and Himawari-8. On the other hand, Sentinel-3 AOD did not appear to have a good quality because it was recently launched and not sufficiently optimized yet, according to ESA (European Space Agency). The AOD of MODIS, VIIRS, and Himawari-8 did not show a significant difference in accuracy according to season and to urban vs. non-urban regions, but the mixed pixel problem was partly found in a few coastal regions. Because AOD is an essential component for atmospheric correction, the result of this study can be a reference to the future work for the atmospheric correction for the Korean CAS (Compact Advanced Satellite) series.

Consistency Analysis of Intermediate Soil Based on the Fines Contents (세립분 함유율에 따른 중간토의 컨시스턴시 분석)

  • Oh, Sewook;Bae, Wooseok;Bang, Seongtaek
    • Journal of the Korean GEO-environmental Society
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    • v.22 no.6
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    • pp.17-26
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    • 2021
  • Ground investigation and result analysis generally used to examine all sorts of structures' subsidence or stability can be classified into sandy soil and cohesive soil, and analysis on the liquid limit of soil is utilized to evaluate the physical properties of ground and types or technical behavior of soil. The most widely used method to analyze liquid limit is Casagrande with which liquid limit can be calculated relatively easily; however, it is fairly difficult to apply it to soil equipped with intermediate properties. Therefore, concerning the properties of soil having the intermediate properties of sedimentary ground, this researcher mixed the clay from Yangsan, Gwangyang, and Busan with sandy soil to make intermediate soil and then carried out the test of consistency limit and also evaluated applicability by using the suggested formula of consistency revision. The sample of intermediate soil was the mixture of clay and sandy soil, and to produce intermediate soil, the content (Fc) of fine soil was applied as 50%, 75%, or 100%. Regarding the physical properties of intermediate soil, to maintain the properties of clay in the natural state, bentonite was added at a fixed rate for controlling the properties of clay, and then, consistency was analyzed. By adopting the formula of consistency revision suggested in advanced research, this author analyzed consistency based on the experiment and consistency based on the suggested formula of revision. Also, about intermediate soil collected at the site, consistency based on the experiment and consistency based on the suggested formula of revision were analyzed comparatively, and about intermediate soil collected, this researcher analyzed particle size and calculated the content (Fc) of fine soil to analyze intermediate soil in diverse conditions. Moreover, about intermediate soil collected at the site, the suggested formula of consistency revision was applied to calculate the compression index, and the compression index based on the experiment and the compression index based on the suggested formula were analyzed comparatively to evaluate the applicability of the suggested formula.

A Study of Habitat Environment Mapping Using Detailed Bathymetry and Seafloor Data in the Southern Shore of the East Sea(Ilsan Beach, Ulsan) (정밀 해저지형 및 해저면 자료를 활용한 동해 남부 연안(울산 일산해변) 생태계 서식지 환경 맵핑 연구)

  • Choi, SoonYoung;Kim, ChangHwan;Kim, WonHyuck;Rho, HyunSoo;Park, ChanHong
    • Economic and Environmental Geology
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    • v.54 no.6
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    • pp.717-731
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    • 2021
  • We analyzed the characteristics of the habitat environment for the Seonam study area in Ulsan, the southern shore of the East Sea using bathymetry and seafloor environment data. The depth of the study area ranges from about 0 m to 23 m. In the west of the study area, the water depth is shallow with a gentle slope, and the water depth becomes deeper with a steep slope in the east. Due to the right-lateral strike-slip faults located in the continental margin of the East Sea, the fracture surfaces of the seabed rocks are mainly in the N-S direction, which is similar to the direction of the strike faults. Three seafloor types (conglomeratic-grained sandy, coasre-graiend sandy, fine-grained sandy) and rocky bottom area have been classified according to the analyses of the bathymerty, seafloor image, and surface sediment data. The rocky bottom areas are mainly distributed around Seaoam and in the northern and southern coastal area. But the intermediate zone between Seonam and coastal area has no rocky bottom. This intermediate area is expected to have active sedimentation as seawater way. The sandy sediments are widely distributed throughout the study area. Underwater images and UAV images show that Cnidarians, Brachiopods, Mollusks are mostly dominant in the shallow habitat and various Nacellidae, Mytilidae live on the intertidal zone around Seonam. Annelida and Arthropod are dominant in the sandy sediments. The distribution of marine organism in the study area might be greatly influenced by the seafloor type, the composition and particle size distribution of the seafloor sediments. The analysis of habitat environment mapping with bathymetry, seafloor data and underwater images is supposed to contribute to the study of the structure and function of marine ecosystem.

Quality Characteristics and Antioxidant Activity of Black Soybean Sunsik Product with Functional Food Ingredients and Functional Labeling System (기능성 원료를 첨가하여 기능성 표시제도를 적용한 검은콩 선식 제품의 품질 특성 및 항산화활성)

  • Lee, Kang-Pyo;In, Ye-Won;Lim, June seok;Cho, Geun hee;Lee, Ok-Hwan;Lee, Boo-Yong
    • Journal of Food Hygiene and Safety
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    • v.37 no.5
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    • pp.339-346
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    • 2022
  • This study was performed to investigate the quality characteristics and antioxidant activity of black soybean Sunsik product with functional food ingredients and functional labeling system. We prepared black soybean Sunsik (BS) containing black beans, cereals, and vegetables. Black soybean Sunsik with nondigestible maltodextrin and calcium lactate (BSN) was prepared by adding non-digestible maltodextrin and calcium lactate to the base recipe to apply a functional labeling system. The particle size in BS was 118.00 ㎛, whereas BSN was 127.00 ㎛. The respective L, a, and b color values of BS were 73.25, 2.36, and 14.21. The respective L, a, and b values of BSN were 73.21, 2.36, and 14.31. The respective water retention capacities of BS and BSN were 241.67% and 216.33%. No significant difference was observed between BS and BSN in the three physicochemical properties described above. However, the pH of BSN was 5.45, which was significantly lower than that of BS. The total respective phenolic contents of BS and BSN were 1.75 mg GAE/g and 1.61 mg GAE/g, and total respective flavonoid contents of BS and BSN were 6.36 mg RE/g and 5.95 mg RE/g. The antioxidant capacities of BS and BSN were compared via assays of DPPH and ABTS radical scavenging activities, FRAP, and reducing power. The antioxidant activities of BS and BSN increased in a dose-dependant manner. No significant difference between BS and BSN was observed in any measure of antioxidant capacity. These results suggested that the addition of functional food ingredients (non-digestible maltodextrin and calcium lactate) did not affect the quality characteristics and antioxidant activity of black soybean Sunsik.

LC-MS/MS analysis and anti-inflammatory effects of crude extract from Coptidis Rhizoma (황련 추출물의 LC-MS/MS 분석 및 항염증 효과)

  • Min-Jung, Kim;Ye-Jin, Yang;Kwang-Youn, Kim;Hun Hwan, Kim;Jae Dong, Son;Ju-Hye, Yang;Dong bin, Lee;Woo Hyun, Kim;Hu-Jang, Lee;Seon Been, Bak;Kwang-Il, Park
    • Herbal Formula Science
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    • v.31 no.1
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    • pp.1-10
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    • 2023
  • Objectives : The main aim of this study was to examine the LC-MS/MS used to identify phenolic compounds of CRE(Coptidis Rhizoma 70% EtOH Extract). Also, we investigated antioxidative activities and Anti-inflammatory activities. Methods : LC-MS/MS Analysis HPLC and LC-MS/MS were performed on a 1260 series HPLC system (Agilent Technologies, Inc., California, USA) and 3200 QTrap tandem mass system (Sciex LLC) operated in positive ion mode (spray voltage set at -4.5 kV). The solvent used was DW and Acetonitrile containing 0.1% formic acid, a gradient system was used at a flow rate of 0.5 mL/min for analysis, and a Prontosil C18 column (length, 250 mm; inner diameter, 4.6 mm; particle size, 5 ㎛; Phenomenex Co., Ltd., California, USA, Biochoff Chromatography) was used. The solvent conditions used in the mobile phases were 0-10 min at 10-15% B, 10-20 min at 20% B, 20-30 min at 25%, 30-40 min at 40%, 40-50 min at 70%, 50-60 min at 95%, and 60-70 min at 95%. The analysis was performed at a wavelength of 284 nm and a temperature of 35℃. The cell viability was measured using a 3-(4,5-dimethyethiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. We examined the effects of CRE on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in a RAW 264.7 cells Results : The chemical analysis CRE by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed that Rosmarinic acid, Ferrulic acid, 3-O-feruloylquinic acid, and 5-O-feruloylquinic acid as phenolic components. DPPH radical scavenging activity was the inhibitory activity of CRE showed at 200 ㎍/mL a statistically significant level. MTT assay demonstrated that the CRE did not have a cytotoxic effect in RAW 264.7 and LPS-induced RAW264.7 cells. Also, CRE reduced NO production in RAW 264.7 cells stimulated with LPS. Conclusions : Based on these findings, The chemical analysis 4 major components CRE such as Rosmarinic acid, Ferrulic acid, 3-O-feruloylquinic acid, and 5-O-feruloylquinic acid. Moreover, we confirmed that CRE has effects antioxidant and anti-inflammatory. The results demonstrate that CRE can be used as an antioxidant and a powerful chemopreventive ingredient against inflammatory diseases.

Geometry and Kinematics of the Northern Part of Yeongdeok Fault (영덕단층 북부의 기하와 운동학적 특성)

  • Gwangyeon Kim;Sangmin Ha;Seongjun Lee;Boseong Lim;Min-Cheol Kim;Moon Son
    • Korean Journal of Mineralogy and Petrology
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    • v.36 no.1
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    • pp.55-72
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    • 2023
  • This study aims to identify the fault zone architecture and geometric and kinematic characteristics of the Yeongdeok Fault, based on the geometry and kinematic data of various structural elements obtained by detailed field survey and anisotropy of magnetic susceptibility (AMS) of the fault rocks. The Yeongdeok Fault extends from Opo-ri, Ganggu-myeon, Yeongdeok-gun to Gilgok-ri, Maehwa-myeon and Bangyul-ri, Giseong-myeon, Uljin-gun, and cuts various rock types from the Paleo-proterozoic to the Mesozoic with a range of 4.6-5.0 km (4.77 km in average) of right-lateral offset or forms the rock boundaries. The fault is divided into four segments based on its geometric features and shows N-S to NNW strikes and dips of an angle of ≥ 54° to the east at most outcrops, even though the outcrops showing the westward dipping (a range of 54°-82°) of fault surface increase as it goes north. The Yeongdeok Fault shows the difference in the fault zone architecture and in the fault core width ranging from 0.3 to 15 m depending on the bedrock type, which is interpreted as due to differences in the physical properties of bedrock such as ductility, mineral composition, particle size, and anisotropy. Combining the results of paleostress reconstruction and AMS in this and previous studies, the Yeongdeok Fault experienced (1) sinistral strike-slip under NW-SE maximum horizontal principle stress (σHmax) and NE-SW minimum horizontal principle stress (σHmin) in the late Cretaceous to early Cenozoic, and then (2) dextral strike-slip under NE-SW maximum horizontal principle stress (σHmax) and NW-SE minimum horizontal principle stress (σHmin) in the Paleogene. It is interpreted that the deformation caused by the Paleogene dextral strike-slip movement was the most dominant, and the crustal deformation was insignificant thereafter.

Meiobenthic community structure in the coastal area of Hallyeohaesang National Park (한려해상국립공원 해역에 서식하는 중형저서동물의 계절별 군집 변동 특성)

  • Teawook Kang
    • Korean Journal of Environmental Biology
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    • v.40 no.2
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    • pp.125-137
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    • 2022
  • To assess the characteristics of meiofaunal community fluctuations related to environmental factors, seasonal surveys were conducted in the subtidal zone of Hallyeohaesang National Park. The average depth of the study area was about 20 m, and the average water temperature at the bottom was low in winter(11.33℃) and high in summer(17.95℃). The sedimentary particles mainly comprised silt and clay at most stations. The abundance of meiofauna ranged from 81.7 to 1,296.5 Inds. 10 cm-2, and the average abundance was 589.3 Inds. 10 cm-2. The average abundance of meiofauna in each season was the lowest at 416.5 Inds. 10cm-2 in winter and the highest at 704.5Inds.10 cm-2 in spring. The dominant taxa were nematodes (about 92%) and harpacticoids (about 5%). In the cluster analysis of meiofaunal communities, they were divided into four significant groups. The largest group mainly contained spring and summer samples, and contained stations with a high nematode density of over 500 Inds. 10 cm-2 and harpacticoids below 50 Inds. 10 cm-2 with a high composition ratio of nematodes. In the cluster analysis, no regional division was found between the stations, and it was thought to be divided by the seasons with high abundance according to seasonal variation and the composition ratio of nematodes and harpacticoids. In the Spearman rank correlation analysis, the density of total meiofauna and the most dominant taxa, nematodes, was not significantly related to environmental factors. However, the density of harpacticoids had a significant positive correlation with water depth and a negative correlation with sediment particle size.

Effect of Oil in Water Nanoemulsion Containing a Mixture of Lactic Acid and Gluconolactone for Skin Barrier Improvement (유산 및 글루코노락톤 혼합물을 함유하는 수중유형 나노에멀젼의 피부장벽개선 효과)

  • Ji-Hye Hong;Young Duck Choi;Gye Won Lee;Young Ho Cho
    • Journal of Life Science
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    • v.33 no.11
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    • pp.905-914
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    • 2023
  • To evaluate the effectiveness of the skin barrier improvement of lactic acid (LA) and gluconolactone (GL), the expression of filaggrin, loricrin, hyaluronic acid (HA), hyaluronan syhthase-2 (HAS2), and aquaporine-3 (AQP3) in keratinocytes, and the moisture content and transepidermal water loss (TEWL) by clinical trials were evaluated. The expression levels of filaggrin and locricrin, which are the main factors affecting the proper functioning of skin barrier function, and HA, HAS2, and AQP3, which are skin moisturizing-related proteins measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. The results showed that the expression levels of the factors that decreased by H2O2 treatment were significantly increased by LA, GL, and a mixture of LA and GL at the mRNA and protein levels (p<0.05). The nanoemulsion containing a mixture of LA and GL was prepared using the emulsion inversion method, and the average particle size was 299.9 ± 0.287 nm. After measuring the TEWL of nanoemulsion using Vapometer, it was found that TEWL significantly decreased by 15.53% and 26.73% after two weeks and four weeks of product use, respectively, compared to TEWL before product use (p<0.001). Similarly, the skin moisture content of the nanoemulsion significantly increased by 15.40% and 26.59% after two weeks and four weeks of product use, respectively, compared to skin moisture content before product use (p<0.001). Therefore, the skin barrier function and moisturizing effect of a mixture of LA and GL are shown by increasing the moisture content and decreasing the TEWL by increasing the expression of filaggrin, loricrin, HA, HAS2, and AQP3. This suggests the possibility for the development of functional cosmetic ingredients in the future.

Review of applicability of Turbidity-SS relationship in hyperspectral imaging-based turbid water monitoring (초분광영상 기반 탁수 모니터링에서의 탁도-SS 관계식 적용성 검토)

  • Kim, Jongmin;Kim, Gwang Soo;Kwon, Siyoon;Kim, Young Do
    • Journal of Korea Water Resources Association
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    • v.56 no.12
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    • pp.919-928
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    • 2023
  • Rainfall characteristics in Korea are concentrated during the summer flood season. In particular, when a large amount of turbid water flows into the dam due to the increasing trend of concentrated rainfall due to abnormal rainfall and abnormal weather conditions, prolonged turbid water phenomenon occurs due to the overturning phenomenon. Much research is being conducted on turbid water prediction to solve these problems. To predict turbid water, turbid water data from the upstream inflow is required, but spatial and temporal data resolution is currently insufficient. To improve temporal resolution, the development of the Turbidity-SS conversion equation is necessary, and to improve spatial resolution, multi-item water quality measurement instrument (YSI), Laser In-Situ Scattering and Transmissometry (LISST), and hyperspectral sensors are needed. Sensor-based measurement can improve the spatial resolution of turbid water by measuring line and surface unit data. In addition, in the case of LISST-200X, it is possible to collect data on particle size, etc., so it can be used in the Turbidity-SS conversion equation for fraction (Clay: Silt: Sand). In addition, among recent remote sensing methods, the spatial distribution of turbid water can be presented when using UAVs with higher spatial and temporal resolutions than other payloads and hyperspectral sensors with high spectral and radiometric resolutions. Therefore, in this study, the Turbidity-SS conversion equation was calculated according to the fraction through laboratory analysis using LISST-200X and YSI-EXO, and sensor-based field measurements including UAV (Matrice 600) and hyperspectral sensor (microHSI 410 SHARK) were used. Through this, the spatial distribution of turbidity and suspended sediment concentration, and the turbidity calculated using the Turbidity-SS conversion equation based on the measured suspended sediment concentration, was presented. Through this, we attempted to review the applicability of the Turbidity-SS conversion equation and understand the current status of turbid water occurrence.