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Analysis of metabolomic patterns in thoroughbreds before and after exercise

  • Jang, Hyun-Jun;Kim, Duk-Moon;Kim, Kyu-Bong;Park, Jeong-Woong;Choi, Jae-Young;Oh, Jin Hyeog;Song, Ki-Duk;Kim, Suhkmann;Cho, Byung-Wook
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.11
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    • pp.1633-1642
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    • 2017
  • Objective: Evaluation of exercise effects in racehorses is important in horseracing industry and animal health care. In this study, we compared metabolic patterns between before and after exercise to screen metabolic biomarkers for exercise effects in thoroughbreds. Methods: The concentration of metabolites in muscle, plasma, and urine was measured by $^1H$ nuclear magnetic resonance (NMR) spectroscopy analysis and the relative metabolite levels in the three samples were compared between before and after exercise. Subsequently, multivariate data analysis based on the metabolic profiles was performed using orthogonal partial least square discriminant analysis (OPLS-DA) and variable important plots and t-test was used for basic statistical analysis. Results: From $^1H$ NMR spectroscopy analysis, 35, 25, and 34 metabolites were detected in the muscle, plasma, and urine. Aspartate, betaine, choline, cysteine, ethanol, and threonine were increased over 2-fold in the muscle; propionate and trimethylamine were increased over 2-fold in the plasma; and alanine, glycerol, inosine, lactate, and pyruvate were increased over 2-fold whereas acetoacetate, arginine, citrulline, creatine, glutamine, glutarate, hippurate, lysine, methionine, phenylacetylglycine, taurine, trigonelline, trimethylamine, and trimethylamine N-oxide were decreased below 0.5-fold in the urine. The OPLS-DA showed clear separation of the metabolic patterns before and after exercise in the muscle, plasma, and urine. Statistical analysis showed that after exercise, acetoacetate, arginine, glutamine, hippurate, phenylacetylglycine trimethylamine, trimethylamine N-oxide, and trigonelline were significantly decreased and alanine, glycerol, inosine, lactate, and pyruvate were significantly increased in the urine (p<0.05). Conclusion: In conclusion, we analyzed integrated metabolic patterns in the muscle, plasma, and urine before and after exercise in racehorses. We found changed patterns of metabolites in the muscle, plasma, and urine of racehorses before and after exercise.

The Clinical Study on 120 Cases with Traffic Accident (교통사고 환자 120례에 대한 임상적 고찰)

  • Lee, Ji-Eun;Jung, Hyo-Keun;Ryu, Chung-Ryul;Cho, Myoung-Rae;Wi, Jun;Ryu, Mi-Seon;Kim, Ja-Young
    • Journal of Acupuncture Research
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    • v.25 no.6
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    • pp.135-143
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    • 2008
  • Objectives : The main purpose of this research is to make a survey of the effective way of the Oriental medical care and to enlarge the range of Oriental medical treatment about traffic accidental patients. Methods : In following research, we surveyed 120 patients of traffic accident who were hospitalized in Dong-Shin Oriental Medical Hospital from October, 1, 2007 to August, 20, 2008 according to medical chart. Results : 1. In distribution of gender and age, the rate of male was 35.8%, that of female was 64.2% and the majority of the patients were twenties and thirties. 2. In the traffic accident patterns, the most was car crash from behind(61.7%) and after traffic accident, 75 patients(62.5%) visited our hospital for oriental medical treatment in less than 5days. 3. 46 patients(38.3%) choose the oriental medical treatment for the primary care and after medical care, 36 patients(31.7%) visited our hospital due to dissatisfaction of current treatment and maintained pain. 4. Partial pain was the chief complaint and the most of painful parts was neck(76.7%), followed by low back(69.2%). 5. Though radiation test, the most was cervical spine sprain(76.7%), followed by straightening of lumbar spine(66.7%). 6. In the periods of admission, less than 7 days was the most(53.3%), followed by less than 14 days(35.9%). 7. The treatment was effective to 93 patient out of 106 who had been hospitalized for less than 14 days. Conclusions : This data suggested that the Oriental medical care could be more effective treatment about traffic accidental patients without surgical injury.

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Effects of CO and $CO_2$ on Hydrogen Permeation through Pd-coated V-Ti-Ni Alloy Membranes (Pd 코팅된 V-Ti-Ni 합금 분리막을 통한 수소투과에서 CO와 $CO_2$의 영향)

  • Jeon, Sung-Il;Park, Jung-Hoon;Lee, Yong-Taek
    • Membrane Journal
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    • v.21 no.3
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    • pp.290-298
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    • 2011
  • The influence of co-existing gases on the hydrogen permeation was studied through a Pd-coated $V_{53}Ti_{26}Ni_{21}$ alloy membrane. The hydrogen permeation characteristics of Pd-coated $V_{53}Ti_{26}Ni_{21}$ alloy membrane have been investigated in the pressure range 1-3 bar under pure hydrogen and hydrogen mixture gas with carbon dioxide and carbon monoxide at $450^{\circ}C$. Preliminary hydrogen permeation experiments have been confirmed that hydrogen flux was $5.36mL/min/cm^2$ for a Pd-coated $V_{53}Ti_{26}Ni_{21}$ alloy membrane (thick: 0.5 mm) using pure hydrogen as the feed gas. In addition, hydrogen fluxes were 4.46, 5.20, $3.91mL /min/cm^2$ for$V_{53}Ti_{26}Ni_{21}$ alloy membrane using $H_2/CO_2$, $H_2/CO$ and $H_2/CO_2/CO$ as the feed gas respectively. Therefore, the hydrogen permeation flux decreased with decrease of hydrogen partial pressure irrespective of temperature and pressure when $H_2/CO_2$, $H_2/CO$ and $H_2/CO_2/CO$ mixture applied as feed gas respectively and permeation fluxes were satisfied with Sievert's law in different feed conditions. It was found from XRD results after permeation test that the Pd-coated $V_{53}Ti_{26}Ni_{21}$ alloy membrane had good stability and durability for various mixtures feeding condition.

The Effect of Travel Motivation on Satisfaction for the Walking Tourist (도보여행객의 여행동기와 만족의 영향관계연구)

  • Son, Ha-Na;Yhang, Wii-Joo
    • Asia-Pacific Journal of Business
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    • v.6 no.2
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    • pp.81-95
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    • 2015
  • This study is an investigative study into the effects that the 'travel motivations' of participants on walking tourism has on their 'degree of satisfaction' by identifying tourist motivations for selecting walking tour among many types of tourism and their effects on hikers' degree of satisfaction. This study was conducted with hikers affiliated with 'walking on beautiful roads '-one of Busan's preliminary social enterprises- with a total of 176 copies used for a final effective sample. Data collected was analyzed using SPSS WIN 18.0 to conduct a frequency analysis of the sample. Along with exploratory factor analysis, Cronbach's ${\alpha}$ coefficient was used as an estimate of the validity and reliability of each factor. Multiple regression analysis was done for an analysis of travel motivations and their effects on satisfaction. The scale rates items in the questionnaire in the following categories: 'Motivations' for walking tours consist of 5 factors- vacation, soul-searching, social get-togethers, nature tour, community experience-with a total of 20 items. 'The degree of satisfaction' is made up of a factor with a total of 4 items. The results of the study are as follows: with respect to the hypothesis that motivations for walking tour would have a positive effect on the degree of satisfaction, test results show that 'motivations' have significant partial effects on the 'degree of satisfaction'. Motivations affect the degree of satisfaction in the order of nature tour and soul-searching except for experience and social get-togethers- an indication that participants on walking tours tend to travel for the attractiveness of nature, wanting time for reflection and meditation. As the first investigative study into participants in walking tours products offered by travel agencies, the study has the following implications for marketers: First, travel agencies selling walking tour products and government institutions concerned about the development of hiking trails need to discuss ways to make the most of natural resources as they are- rather than using development-oriented approaches. The study has its own limitation: it is that with the lack of domestic and international studies on walking tours, the study has not gained access to precedent studies on the subject so it has not established a systemic approach to the emerging type of tourism. Hopefully, this study will make a small contribution to its development.

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Effects of a older volunteer activity on the career development of girl's middle school student -Career attitude maturity, Career decision level- (노인봉사활동이 여중생의 진로발달에 미치는 영향 -진로태도성숙도, 진로결정수준을 중심으로-)

  • Lee, Jae-Cheol;Kweon, Nak-Won;Yoon, Sung-Cheol
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.10
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    • pp.4322-4327
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    • 2011
  • This study was conducted to analyze the influences of older volunteer activity. In detail, Administered on girl's maddle school students with the program's purpose on encouragement of positive career development. The study sample was taken from 30 students attending Y girl's middle school in Gyeonggi province, all of whom were regular volunteers at a near by rest home. The contents of the study and apparatus alike incorporated the use of a CAMI and CDS employed as a means of observing the changes before and after older volunteer activity experience in relation to five sub factors and career decision level. The data were analyzed by t-test using the SPSS Program. The results were as follows: First, it appears that older volunteer activity, in general, partial effect of improving a girl's middle school students' career attitude maturity. Second, it appears that older volunteer activity, improving effect a girl's middle school students' career decision level. Third, a girl's middle school students' career attitude maturity and career decision level had positive to advance more in particular in cases where the students' family also participated in older volunteer activity. As observed by the above results, a older volunteer activity experience should be an integral to a girl's middle school students' career education program plan as an effective means of enhancing their career attitude maturity and career decision level.

Congenital Dropsy in Korean-native Calf (한우 송아지의 선천성 전신수종)

  • 강문일;박영석;한동운
    • Korean Journal of Animal Reproduction
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    • v.23 no.1
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    • pp.63-68
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    • 1999
  • A 4-year old Korean-native cow of third parturition delivered an deformed calf with congenital anasarca and hypoplasia of the extremity at Muan County in Chonnam Province. At parturition, the dam showed amniotic fluid approximately two times more than that at the normal and also occurred severe dystocia. The anomaly died before parturition was 82 cm long and weighed 25 kg. It was difficult to recognize the neck and face of the anomaly due to severe edema and the stretched tongue through mouth was found. The shape of the extremity was recognizable and the systemic hypoplasia of the body with marked edema was found. At necropsy, there was a large amount of serous fluid both in pleural and peritoneal cavity. Also the fluid was contained some of yellowish granular materials and diffusely was fulfilled in all subcutaneous tissue. There was a partial opening in diaphragm which was resulted in the protrusion of approximately half of liver toward peritoneal cavity. There were two kidneys (15${\times}$21 and 13${\times}$18 cm) on the left which were enlarged and relatively larger than the right one (13${\times}$9 cm). In addition, there were found slight to moderate hepatomegaly and splenomegaly with slight congestion, and diffuse edema of intestinal serosa. Lung (32${\times}$49 mm) with marked hypoplasia was smaller than the size of heart (56${\times}$45mm). Serological test to the dam showed relatively high antibody titer (>64) to Akabane virus which might be involved in the outbreak of the deformed Korean-native calf as one of many causative agents.

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Effects of Bispectral Index Monitoring Based Sedative Administration on Conscious Sedation, Physiological Stability and Recovery Time in Patients Receiving Endoscopic Submucosal Dissection (이중분광계수 모니터기반 진정제 투여가 내시경 점막하 박리술 환자의 의식하 진정상태, 생리적 안정성 및 회복시간에 미치는 효과)

  • Lee, Mi Jeong;Hwang, Moon Sook;Lim, Hyun Sook;Park, Mi Ok;Huh, Ji Won;Kang, Ki Joo;Kim, Jae Jun;Cho, Myung Sook
    • Journal of Korean Clinical Nursing Research
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    • v.18 no.2
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    • pp.284-295
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    • 2012
  • Purpose: This study was done to clarify effects of bispectral index monitoring sedative administration, compared to MOAA/S (Modified Observer's Assessment of Alertness and Sedation), on conscious sedation, physiological stability and recovery time for patients undergoing endoscopic submucosal dissection. Methods: Participants In this study were patients who underwent endoscopic submucosal dissection because of early gastric cancer. Participants were assigned randomly to an experimental group receiving sedatives based on bispectral index monitoring or to a control group with the MOAA/S instrument. Movements, belching, memory, pain, discomfort, physiological stability (MBP, PR, $SpO_2$), and recovery time were measured during the treatment and recovery. Data were analyzed using Spearman partial correlation coefficient analysis, Mixed model and Wilcoxon rank sum test to determine differences in the parameters. Results: There were no statistically significant differences between the two groups for conscious sedation(movement, belching, memory, pain, or discomfort), physiological stability and recovery time. Conclusion: The results of this study indicate that no differences were found between the two types of monitoring. Thus, use of a bispectral index monitor in clinical practice enabling medical staff to readily assess the conscious sedation of for these patients is expected to be increasingly used as an objective assessment tool for conscious sedation for patient safety.

A Study on the Experiment of Represtressed Preflexional Composite Beams (RPF 합성보의 실험 연구)

  • Chang, Dong Il;Hwang, Yoon Kook;Kim, Jung Ho;Cho, Tae Jun
    • Journal of Korean Society of Steel Construction
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    • v.9 no.4 s.33
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    • pp.697-705
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    • 1997
  • The conventional preflex beams are designed by the method of paritial prestressing and allow the tensile stresses at the lower concrete of beams. As a consequence, most of preflex beams experience the tensile cracks under the service loads. This study was conducted to develop the most effective preflex beams, which do not allow tensile stress under the service load, by introducing additional prestressing called 'represtressing' at the lower concrete of beams. The objective of the study was accomplished by developing a computer analysis and design program and conducting experiments. Using the developed computer program, standard sections of the represtressed preflex beams were determined by computer modeling. In the experiment, two actual size of represtressed beams were tested under the imitated service loads. The results of test have shown that the performance of the represtressed preflex beams is generally excellent. A remarkable improvement was made in the design of preflex composite beams. Since the represtressed preflex beams(RPF) do not experience the tensile cracks under the service loads, the use of this beam for the bridge structures will lead to easy bridge maintenance and management. Furthermore, due to the low beam depth, high clearance and economical design can be realized in the bridge design using RPF.

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The Relationship between Offline Trust and Online Transaction in Internet Banking (인터넷 뱅킹에서 오프라인 신뢰와 온라인 거래의 관계)

  • Lee, Woong-Kyu
    • Asia pacific journal of information systems
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    • v.17 no.2
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    • pp.29-47
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    • 2007
  • Owing to the rapid growth of using the Internet, not only click-and-mortar companies but also brick-and-mortar ones have been expanding their distribution channels into online, Moreover, since online channels are more attractive than offline ones in control and maintenance, switching customers into online ones is emerged as one of very important managerial issues in a view of reduction of cost as well as expansion of services. However, the switched customers should be faced by uncertainties which could not have been experienced in offline. Specifically, in online channels, buyers and sellers are separated temporally and spacially and there are always so many kinds of threat for security as well as not enough systems and conventions for them yet. Therefore, trust has been considered as one of the most critical mechanisms for resolution of such uncertainties in online transactions. However, it is not easy to build and maintain the relationships in online since most of them are virtual and indirect generally. Therefore, in order to switch offline customers into online ones, it is very important to make strategies based on identification of the relationship between online transaction and offline trust which has been built in offline business. Generally offline trust, which has been built independent of online, could not include trust for online-dependent activities such as payment security during or after transactions, while most of online trust include it. Therefore, a customer with high offline trust does not always perceive high security and assure safe transactions. Accordingly, while online trust, where technical capabilities for online security is one of main bases, includes control trust implicitly or explicitly, offline trust does not. However. in spite of such clear discrimination and independence between offline trust and perceived security, there can be the significant dependency between these two beliefs. The customers with high offline trust believe that the company would do some activities for online security for customers' safe transactions since it has been believed of doing well for customers' trust. Theoretically, users' perception of security is interpreted as a kind of control trus, which is trust for company's technical control capacities in order to resolve technical uncertainties in online. Therefore, the relationship between two beliefs can be considered as transference from offline trust to another type trust. that is, control trust. The objective of this study is to analyze the effect of offline trust on online transaction uses mediated by perceived security. For this purpose, we suggest a research model based on technology acceptance model (TAM). Reuse intention is adopted as a dependent variable and TAM is modified by adding perceived risk (PR) as well as two beliefs of using Internet banking, perceived usefulness (PU) and perceived ease of use (PEOU). Moreover, perceive security (PS) is adopted as an external variable for PR and PU, while offline trust (OT) is an antecedent of PS. For an empirical test, sampling from 108 visitors to the banks in Daegu, Korea, we analyze our model by partial least square (PLS) approach. In result, our model is shown to explain 51.4% of the variance in reuse intention and all hypothesis are supported statistically. A theoretical implication of this study is to identify a role of PS between offline trust and reuse intention of using online transaction services. According to our result, PS can be considered as a mediation variable for bridging between two different concepts: trust that explains social aspects of customers and companies, and TAM that explains customers' reuse intention.

Effect of Body Exposure and Color of Suit on the Impression of Modesty (의복의 색과 신체노출이 정숙성인상에 미치는 영향)

  • Koh AeRan;Kahng Hewon
    • Journal of the Korean Society of Clothing and Textiles
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    • v.16 no.3 s.43
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    • pp.181-195
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    • 1992
  • The purposes of this study were to investigate 1) the effect of body exposure and color of a woman's suit on the perception of modesty, and 2) the effect of perceiver's sex and age on impression formed by the function of clothing variables. The instrument of this study consisted of a response scale and stimuli. Thirteen items of 7-point semantic differential scales were developed to measure the perceiver's impression on wearer's modesty. Stimuli were color pictures of a model wearing one of 8 types of suit constructed by a 2 $\times$ 2 $\times$ 2 factorial design. The manipulation of each level of the clothing variables were: color of the suit by black and red, leg exposure by varying skirt lengths to a Chanel-line and mini skirt, and neck exposure by shirt collar blouse and scarf. Two models, representing typical female college students living in Seoul, were selected to eliminate model effect. The sample include 384 subjects, consisting of 4 groups of male and female college students and middle aged men and women. Eight experimental groups were randomly assigned to one of eight stimuli based on between-subject design. One half of each group responded to model 1 and the other half to model 2 of same stimulus. Responses to the semantic differential scales were factor analyzed (pc model, Varimax rotation) to identify factors constructing impression of modesty. Two factors emerged regard­less of subgroups; Elegance and Extroversion factor. The first factor was found to be dominant, accounting for 60 percent of the total variance. The other accounted for just 11 percent. Multidimensional ANOVA (5-way, 3-way) was conducted to test the effect of the clothing variables against two factors identified from the factor analysis. Leg exposure was the most powerful variable affecting the impression of Elegance and Extroversion factor for all per. ceiver subgroups. Neck exposure had primary effect on the impression of Elegance, whereas it partially influenced that of Extroversion. Color of suit had only partial effect on the impression of Extroversion. Hypothesis I was partially supported from the findings above. The effect of perceiver's age and sex on impression by the function of clothing variables was tested by comparing the result between four subgroups. In forming an impression of the wearer's modesty, male college students were least affected by the manipulation of clothing variables, while middle aged males were affected most. In the female groups, there was no age difference and they fell between the male groups in the degree to which they were affected. Hypothesis II was supported only by age difference in two male groups, and by sex difference in two student groups.

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