• Title/Summary/Keyword: Park Hankyung

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Paromomycin Derived from Streptomyces sp. AG-P 1441 Induces Resistance against Two Major Pathogens of Chili Pepper

  • Balaraju, Kotnala;Kim, Chang-Jin;Park, Dong-Jin;Nam, Ki-Woong;Zhang, Kecheng;Sang, Mee Kyung;Park, Kyungseok
    • Journal of Microbiology and Biotechnology
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    • v.26 no.9
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    • pp.1542-1550
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    • 2016
  • This is the first report that paromomycin, an antibiotic derived from Streptomyces sp. AG-P 1441 (AG-P 1441), controlled Phytophthora blight and soft rot diseases caused by Phytophthora capsici and Pectobacterium carotovorum, respectively, in chili pepper (Capsicum annum L.). Chili pepper plants treated with paromomycin by foliar spray or soil drenching 7 days prior to inoculation with P. capsici zoospores showed significant (p < 0.05) reduction in disease severity (%) when compared with untreated control plants. The disease severity of Phytophthora blight was recorded as 8% and 50% for foliar spray and soil drench, respectively, at 1.0 ppm of paromomycin, compared with untreated control, where disease severity was 83% and 100% by foliar spray and soil drench, respectively. A greater reduction of soft rot lesion areas per leaf disk was observed in treated plants using paromomycin (1.0 μg/ml) by infiltration or soil drench in comparison with untreated control plants. Paromomycin treatment did not negatively affect the growth of chili pepper. Furthermore, the treatment slightly promoted growth; this growth was supported by increased chlorophyll content in paromomycin-treated chili pepper plants. Additionally, paromomycin likely induced resistance as confirmed by the expression of pathogenesis-related (PR) genes: PR-1, β-1,3-glucanase, chitinase, PR-4, peroxidase, and PR-10, which enhanced plant defense against P. capsici in chili pepper. This finding indicates that AG-P 1441 plays a role in pathogen resistance upon the activation of defense genes, by secretion of the plant resistance elicitor, paromomycin.

Analysis on Trends in the Designation and Development of Historical Parks in Korea (국내 역사공원의 지정 및 조성 경향 분석)

  • Gil, Ji-Hye;Park, Hee-Soung;Park, Jae-Min
    • Journal of the Korean Institute of Landscape Architecture
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    • v.44 no.2
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    • pp.130-142
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    • 2016
  • After the revision of the Urban Park Act in 2005, historical parks emerged in Korea to promote the preservation of historical heritage while also offering recreation and education to ordinary citizens. It is now time to examine the characteristics of domestic historical parks by examining their current operational conditions, and seek appropriate institutional improvements. By studying the characteristics of historical parks in various countries, as well as the trends in the designation and development of domestic historical parks, this study attempts to examine the function and role of historical parks, and seeks a direction for future action. Through its literature review, this study also examines the current state of historical parks through cooperation with relevant public officials and experts. The results of the study show that, despite historical resources being concentrated in sites dating to the Joseon Dynasty, they also include heritage pertaining to persons, events, and places. There is also a trend toward increasing the focus on modern heritage. Historical parks show differences across existing cities and new towns, as well as between major cities and provincial cities. Provincial cities showed a recent trend of using historical parks as important resources for strengthening their economics and solidifying their identities. Also, there are many cases where the designated category for a park is changed to a historical park. In such cases, there may be a problem where certain functions of the park run into conflict. Domestic historical parks can be divided into four categories: heritage parks, memorial parks, historical theme parks, and historic parks. Such detailed classification schemes may serve as the strategic foundation for later conservation and usage of historical heritage, as well as a standard for suggesting concrete direction in the operation of historical parks.

Identification of Genetic Variations in CBL, SORBS1, CRK, and RHOQ, Key Modulators in the CAP/TC10 Pathway of Insulin Signal Transduction, and Their Association with Type 2 Diabetes Mellitus in the Korean Population

  • Hong, Kyung-Won;Jin, Hyun-Seok;Lim, Ji-Eun;Go, Min-Jin;Lee, Jong-Young;Hwang, Sue-Yun;Park, Hun-Kuk;Oh, Berm-Seok
    • Genomics & Informatics
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    • v.7 no.2
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    • pp.53-56
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    • 2009
  • Recent evidence has strongly suggested that the CAP/TC10 pathway is involved in the trafficking, docking, and fusion of vesicles containing the insulin-responsive glucose transporter Glut4 to the plasma membrane. However, little is known about how the genes employed in the CAP/TC10 pathway are associated with the development of type 2 diabetes mellitus. In this study, we sequenced 4 genes of the CAP/TC10 pathway [SORBS1, CBL, CRK, and RHOQ] in 24 individuals to identify genetic variations in these loci. A total of 48 sequence variants were identified, including 23 novel variations. To investigate the possible association with type 2 diabetes mellitus, 3 single nucleotide polymorphisms from SORBS1, 3 from CBL, and 4 from RHOQ were genotyped in 1122 Korean type 2 diabetic patients and 1138 nondiabetic controls. Using logistic regression analysis, 1 significant association between SNP rs1376405 in RHOQ and type 2 diabetes mellitus [OR = 8.714 (C.I. 1.714-44.29), p = 0.009] was found in the recessive model. Our data demonstrate a positive association of the RHOQ gene in the CAP/TC10 pathway with T2DM in the Korean population.

Morphology of Arbuscular Mycorrhizal Roots and Effects of Root Age and Soil Texture on the Mycorrhizal Infection in Panax ginseng C.A. Meyer

  • Lee, Kyung-Joon;Park, Hoon;Lee, In-Sik
    • Journal of Ginseng Research
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    • v.28 no.3
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    • pp.149-156
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    • 2004
  • The objectives of this study were to investigate the morphology of mycorrhizal roots, and the effects of root age and soil texture on the mycorrhizal infection in ginseng (Panax ginseng C. A. Meyer) growing in Korea. Ginseng roots at ages of two to six years were collected from fields in late June. Their infection by arbuscular mycorrhizal fungi(AMF) was studied by clearing the roots and staining fungal hyphae with trypan blue. Root infection varied greatly depending on the developmental stages of young roots. Young tertiary roots, in diameter of smaller than 0.8 mrn, formed during the current growing season had root hairs and were frequently and in some cases heavily infected by AMF. Hyphal coils and arbuscules were abundant, while vesicles were rarely observed. Older secondary or tertiary roots in diameter of bigger than 1.0 mm with fully differentiated primary xylem formed during the previous growing season had no root hairs, and were not infected at all. The rates of mycorrhizal infection in the young tertiary roots were not affected by the age of the ginseng plants, suggesting that fungal populations might have not much changed during the aging of the cultivated fields up to six years. The differences in the infection rates among the different ages of ginseng were caused by differences in the amount of young tertiary roots in the samples. Soil texture, either sandy loam or clay loam, did not affect the rate of root infection. There were large variations in the infection rates among the different farms and locations within a farm. It strongly suggested that infection rates of the ginseng roots by AMF would be influenced by the practice of the farmers, possibly by avoiding consecutive planting, introduction of new topsoil, and the ways of handling the soil before transplanting the ginseng, such as fumigation or sterilization that might have affected indigenous inoculum sources of the AMF.

Adaptive Fuzzy Bilinear Synchronization Control Design for Uncertain $L\ddot{u}$ Chaos System (불확실한 $L\ddot{u}$ 카오스 시스템을 위한 적응 퍼지 Bilinear 동기화 제어 설계)

  • Baek, Jae-Ho;Lee, Hee-Jin;Park, Mig-Non
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.47 no.3
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    • pp.59-66
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    • 2010
  • This paper is proposed an adaptive fuzzy bilinear synchronization design for uncertain $L\ddot{u}$ chaos system. It is assumed that the $L\ddot{u}$ chaos system has unknown parameters. First, The $L\ddot{u}$ chaos system can be reconstructed via TS fuzzy bilinear modeling. We design an adaptive fuzzy bilinear synchronization control scheme based on TS fuzzy bilinear $L\ddot{u}$ chaos system with uncertain parameters. Lyapunov theory is employed to guarantee the stability of error dynamic system between TS fuzzy bilinear $L\ddot{u}$ chaos system and the proposed slave system and to derive the adaptive laws for estimating unknown parameters. Simulation results is given to demonstrate the validity of our proposed synchronization scheme.

Mechanical Behavior of Fruits under Impact Loading (과실의 충격특성에 관한 연구)

  • Hong J. H.;Myung B. S.;Choe J. S.;Kim C. S.;Kim T. W.;Chung J. H.;Park J. W.
    • Journal of Biosystems Engineering
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    • v.30 no.5 s.112
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    • pp.274-279
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    • 2005
  • Impact is one of the major cause of damage to fruits druing varios processes from the production on the farm to the consumer. The tissue of fruits are ruptured in a very short period time less than 10ms by impact loading. Mechanical behavior of fruits under impact loading can be analyzed better with high speed sampling data acquisition system and one of them is a digital storage oscilloscope. A impact test system was developed to test the physical properties of fruits including apple, pear, and peach which may lead to a better understanding of the physical laws. The test system consisted of a digital storage oscilloscope and simple mechanism which can apply impact force to fresh produce. Rupture force, energy, and deffrmation were measured at the five levels of drop heights from 4 to 24cm fur each internal and external tissues. Rupture forces for apple and pear were in the range of 72.9 to 87.7 N and 70.8 to 84.1 N for external and internal tissues, respectively. Rupture forces far peach external tissues were in the range of 43.4 to 65.0 N.

Induced Systemic Resistance by Bacillus vallismortis EXTN-1 Suppressed Bacterial Wilt in Tomato Caused by Ralstonia solanacearum

  • Park, Kyung-Seok;Paul, Diby;Kim, Yong-Ki;Nam, Ki-Woong;Lee, Young-Kee;Choi, Hyo-Won;Lee, Sang-Yeob
    • The Plant Pathology Journal
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    • v.23 no.1
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    • pp.22-25
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    • 2007
  • Biocontrol activity of five strains of selected rhizo-bacteria were tested in tomato against bacterial wilt caused by Ralstonia solanacearum. After root bacterization the plants were grown in a perlite-hydroponic system. Upon challenge inoculation with the pathogen, all of the rhizobacterial strains efficiently suppressed the bacterial wilt in tomato in various rates, at maximum by the strain, Bacillus vallismortis strain EXTN-1. While the percent of infected plants in the non-bacterized control plants were 95%, it was only 65% in plants pre-treated with EXTN-1. It was also demonstrated that the movement of R. solanacearum within the stem was significantly hampered when the plants were root bacterized. As EXTN-1 has no antagonistic properties against R. solanacearum, the bacterial wilt was probably suppressed by a mechanism other than antibiosis. Previously, the strain had been proven to produce an efficient elicitor for inducing systemic resistance in many crops. As the present study confirmed that EXTN-1 has the ability for reducing the pathogen spread in tomato, the strain could be effectively used as a potential biocontrol agent against bacterial wilt.

Evaluation of Cropping Model of Green Manure Crops with Main Crops for Upland-Specific

  • Chung, Doug Young;Park, Misuk;Cho, Jin-Woong;Lee, Sang-Eun;Han, Kwang-Hyun;Ryu, Jin-Hee;Hyun, Seong-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.2
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    • pp.81-86
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    • 2013
  • For organic farming, green manure crops such as leguminous forages and barley have been broadly used to improve soil fertility and soil physical and chemical properties by repeatedly cutting and mulching them directly as winter crop in the field in the rotation. In this investigation we selected 78 agricultural farm corporations as well as individual organic farmhouses related to crop rotation from greenmanure crops to main crops in order to analyze the relationship of cropping system between main crops and green manure crops. The results showed that the green manure crops were divided into two groups as leguminous and nonleguminous crops, representing that those are limited to specific climate and farming systems of regions. Also the 10 or less green manure crops including sudangrass, hairyvetch, italian ryegrass, sorghun, buckwheat, oat, pea, rye, clover, and canola which belong to leguminous crops which are presently cultivated from the organic farmhouses within the rotational crop system. We also confirmed that the major main crops are sweet potato, soybean, corn, tobacco, spinach from usage frequency analyzed by NetMiner H 2.6 which was used to estimate the rotational cropping system among the green manure crops and main crops.

Simultaneous Determination of Pesticide Residues in Soils by Dichloromethane Partition - Adsorption Chromatography - GC-ECD/NPD Analytical Methods (Dichloromethane 분배 - 흡착 크로마토그래피 - GC-ECD/NPD 분석법에 의한 토양잔류농약 다성분 분석)

  • Kim, Chan-Sub;Lee, Byung-Moo;Park, Kyung-Hun;Park, Byung-Jun;Park, Jae-Eup;Lee, Young-Deuk
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.361-370
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    • 2010
  • Considering the efficiencies of the preparation process at each stage obtained in previous studies, the analytical determination method was established for multi-pesticide residues in soils. It consist of the acetone-extraction, the dichloromethane-partition, the Florisil or silica-gel chromatography and the gas chromatography analysis equipped with the electron capture detector and the nitrogen-phosphorus detector. In the soil recovery test by Florisil clean-up system, the number of pesticides recovered in the range of 70~120% and showed less than 20% of RSD were 165 pesticides for paddy soil, 169 pesticides for upland soil and 159 pesticides in both soils through the tested 183 pesticides. And in the soil recovery test by silica-gel system, the number of pesticides recovered in the range of 70~120% and showed less than 20% of RSD were 154 pesticides for paddy soil, 145 pesticides for upland soil, and 134 pesticides in both soils.

Study on the hydrophobic modification of zirconia surface for organic-inorganic hybrid coatings (유-무기 하이브리드 코팅액 제조를 위한 지르코니아 표면의 소수화 개질 연구)

  • Lee, Soo;Moon, Sung Jin;Park, Jung Ju
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.260-270
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    • 2017
  • Zirconia has white color and physical, chemical stability, also using in high temperature materials and various industrial structural ceramics such as heat insulating materials and refractories due to their low thermal conductivity, excellent strength, toughness, and corrosion resistance. If hydrophobically modified zirconia is introduced into a hydrophobic acrylate coating solution, the hardness, chemical, electrical, and optical properties will be improved due to the better dispersibility of inorganic particle in organic coating media. Thus, we introduced $-CH_3$ group through silylation reaction using either trimethylchlorosilane(TMCS) or hexamethyldisilazane(HMDZ) on zirconia surface. The $Si-CH_3$ peaks derived from TMCS and HMDZ on hydrophobically modified zirconia surface was confirmed by FT-IR ATR spectroscopy, and introduction of silicon was confirmed by FE-SEM/EDS and ICP-AES. In addition, the sedimentation rate result in acrylate monomer of the modified zirconia showed the improved dispersibility. Comparison of the sizes of a pristine and the modified zirconia particles, which were clearly measured not by the normal microscope but by particle size analysis, provided a pulverizing was occurred by physical force during the silylation process. From the BET analysis data, the specific surface area of zirconia was approximately $18m^2/g$ and did not significantly change during modification process.