• Journal of Oral Medicine and Pain
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• 제21권2호
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• pp.317-329
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• 1996

Allelic frequency and genotype distribution of short tandem repeat(STR) F13A01 locus was analysed by polymerase chain reaction, polyacrylamide gel electrophoresis and silver staining from human genomic deoxyribonucleic acid(DNA) was extracted from 205 unrelated Korean to be applied to forensic identification and parentage testing as a database. The results were as follows : 1. 5 alleles and 11 genotypes of F13A01 locus were detected and heterozygosity value was 62.0% and the observed each alleles and allelic frequency was 3.2(0.363), 4(0.105), 5(0.063), 6(0.466), 16(0.002). 2. The allelic diversity value was 0.639 and the power of discrimination was 0.804.3. Compared with observed number of alleles and allele frequency in ethnic difference, result was appeared to be similar to that of Japanese and Asians, while was appeared to be much different to that of Blacks and Caucasians in the observed number of alleles and frequency of allele 3.2, 5, 7. From the above result of this investigation, the allelic frequency of STR F13A01 locus in the Korean was considerd to be useful for individual identification and parentage testing as a database.

• 한국임상수의학회지
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• 제26권3호
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• pp.220-225
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• 2009

말에서 개체식별 및 친자확인을 위한 ISAG microsatellite marker의 적용을 위한 유용성 및 표준화를 위해 더러브렛종 6두를 포함한 다양한 품종의 20두를 대상으로 22개의 ISAG microsatellite marker를 분석한 결과 대립유전자의 수는 4개에서 8개로 분포하였고 더러브렛종에서 출현하는 대립유전자의 대부분이 비더러브렛종에서도 출현하였다. 본 연구결과를 통해서 ISAG microsatellte marker를 제주말의 혈통등록에 적용할 수 있음을 알 수 있었다.

• Genomics & Informatics
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• 제16권1호
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• pp.10-13
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• 2018

Until now microsatellite (MS) have been a popular choice of markers for parentage verification. Recently many countries have moved or are in process of moving from MS markers to single nucleotide polymorphism (SNP) markers for parentage testing. FAO-ISAG has also come up with a panel of 200 SNPs to replace the use of MS markers in parentage verification. However, in many countries most of the animals were genotyped by MS markers till now and the sudden shift to SNP markers will render the data of those animals useless. As National Institute of Animal Science in South Korea plans to move from standard ISAG recommended MS markers to SNPs, it faces the dilemma of exclusion of old animals that were genotyped by MS markers. Thus to facilitate this shift from MS to SNPs, such that the existing animals with MS data could still be used for parentage verification, this study was performed. In the current study we performed imputation of MS markers from the SNPs in the 500-kb region of the MS marker on either side. This method will provide an easy option for the labs to combine the data from the old and the current set of animals. It will be a cost efficient replacement of genotyping with the additional markers. We used 1,480 Hanwoo animals with both the MS data and SNP data to impute in the validation animals. We also compared the imputation accuracy between BovineSNP50 and BovineHD BeadChip. In our study the genotype concordance of 40% and 43% was observed in the BovineSNP50 and BovineHD BeadChip respectively.

• 생명과학회지
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• 제16권3호
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• pp.382-386
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• 2006

고양이의 혈통 등록을 위한 개체식별 및 친자판정을 목적으로 microsatellite DNA다형을 조사한 결과 다음과 같은 성적을 얻었다. 고양이 20두를 대상으로 microsatellite DNA다형의 대립유전자를 조사한 본 연구에서는 관찰된 대립유전자의 수는 $3{\sim}8$개 (평균 5.5개)이며 marker별 대립유전자는 FCA005 142bp (0.3750), FCA026 148 bp (0.5500), FCA075 136 bp (0.5000), FCA105 191 bp (0.4250), FCA224 156 bp (0.7750), FCA229 166 bp (0.6500), FCA240 163 bp (0.3000), FCA293 185 bp (0.5000), FCA453 186 bp (0.5500), FCA651 134 bp (0.6750) 대립유전자가 높은 빈도로 관찰되었다. Expected heterozygosity와 PIC는 각각 $0.390{\sim}0.827$(평균 0.639), $0.357{\sim}0.780$(평균 0.581)으로 나타났고 FCA240의 marker는 PIC value가 0.70 이상이었다. 또한 PE는 $0.076{\sim}0.444$으로서 10개 marker를 조합시 total PE는 0.9441로 관찰되었다. 10개의 microsatellite DNA다형 좌위를 가지고 친자관계를 분석한 결과 8두 중에서 1두(12.50%)가 모순으로 판정되었다. 또한 국내에서 사육중인 고양이의 개체식별 및 친자판정에 microsatellite marker를 활용할 수 있을 것으로 사료된다.

• 한국임상수의학회지
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• 제17권1호
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• pp.234-237
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• 2000

The dornor, 2 years old, 20kg and mixed breed, was bred naturally on day 1 and day 3 of estrus and eight gastrulae were collected by flushing the uterus of the donor after laparatomy on day 13 after the second mating. The embryos were frozen by programmable freezer and preserved for about 3 months in liquid nitrogen. Another bitch in natural estrus, 2 years old, 30kg, mixed breed, was selected as the recipient and the frozen embryos(8 gastrulae) were thawed and each 4 embryos were transferred into upper partr of left and right uterine horn, respectively, on day 13 after the proper mating day determined by vaginal smear. The ecipient delivered 6 offspring 48 days after embryo transfer. Of 6 puppies, one was still birth and two puppies died one month after birth. Parentage test was performed by DNA analysis using microsatellite sequences for 3 puppiers, the recipient, the donor, the male dog bred with the donor, and the male dog raised near to the recipient. The markers selected for the test were CXX 873(133-157 base pair) and CXX 894(141-165 base pair). Using primers manufactured according to the markers, the blood samples were processed for polymerase chain reaction and the PCR products were treated for electrophoresis. The three puppies showed identical band to that of recipient, consequently, it was concluded that the puppies were offspring of the recipient mated naturally by the male dog, not the offspring by embryo transfer.

• Journal of Oral Medicine and Pain
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• 제21권2호
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• pp.243-253
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• 1996

In order to be utilized as a database in forensic identification and parentage test, allelic frequency and genotype distribution of short tandem repeat(STR) F13B locus was analysed by polymerase chain reaction in 210 Korean adults who are not related. The results were as follows. 1. 3 alleles and 56 genotypes of F13B locus were detected and heterozygosity value was 48.6% and allelic diversity value was 0.639 and the power of discrimination was 0.804. 2. The observed each alleles and allelic frequency was 8(0.069), 9(0.193), 10(0.738). In conclusion, the allelic frequency of STR F13B locus in the Korean is considered as an useful DNA allelic profile for forensic identification, but it should be used with several other STR locus to get definitive conclusion of analysis for individual identification and parentage testing.

• Journal of Animal Science and Technology
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• 제52권5호
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• pp.357-366
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• 2010

한우의 유전체 전장의 정보를 Illumina BeadArray$^{TM}$ Bovine SNP50 assay를 이용하여 단일염기다형 현상을 조사한 결과, 유전적 다양성을 보이는 좌위가 약 32,567 좌위 이상에서 다양성을 보이고 있었으며 약 5,554 좌위에서 다양성이 조사되지 않았다. 이는 조사된 자료의 가계집단의 수가 크게 제한되었기 때문에 기인될 수 있으며 또 다른 원인으로는 한우 종축집단의 크기가 작을 수 있다는 현상을 반증한다고 사료된다. 유전분석의 기초가 되는 혈통기록에 의한 개체간 혈연관계를 유전체 정보에 의한 혈연관계와 비교하여 본 결과, 유전체 정보에 의한 혈연관계의 크기가 혈통기록에 의한 혈연관계보다 좀 더 정확하게 추정될 수 있다는 장점이 있으며 혈통기록상의 오류로 그릇된 혈연관계의 크기를 유전체 정보를 통하여 보완할 수 있다는 장점이 있다. 이러한 장점을 활용하면 유전체정보를 이용한 유전능력 평가의 정확성을 크게 향상시킬 수 있을 것으로 사료되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1071-1074
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• 2005

Microsatellite polymorphism and their genetic relationships were estimated using genotype information of 183 dogs from 11 microsatellite loci. The breeds include the indigenous Korean breeds Jindo dog (30), Poongsan dog (20) and Miryang dog (44) together with Chihauhau dog (31) and German Shepherd dog (58). Jindo dogs showed the highest expected heterozygosity (0.796${\pm}$0.030) and polymorphic information contents (0.755) in all populations. The phylogenetic analysis showed the existence of two distinct clusters supported by high bootstrap values: the Korean native dogs and other dogs. They clearly show that Poongsan dog and Miryang dog are closely related to each other when compared with Jindo dog. Microsatellite polymorphism data was shown to be useful for estimating the genetic relationship between Korean native dogs and other dog breeds, and also can be applied for parentage testing in those dog breeds.

• Journal of Animal Science and Technology
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• 제45권2호
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• pp.191-198
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• 2003

국내에서 사육중인 치와와 31두, 풍산개 20두, 래브라도 리트리버 8두를 대상으로 micro-satellite DNA형의 유전자 빈도에 기초하여 heterozygosity, PIC, 그리고 PE를 분석한 결과를 요약하면 다음과 같다. 치와와의 대립유전자 수는 4${\sim}$14개로서 expected heterozygosity와 PIC는 각각 0.432${\sim}$0.883 (평균 0.711), 0.397${\sim}$0.856(평균 0.659)으로 나타났으며 PEZ1, PEZ3, PEZ6, PEZ10, PEZ12의 marker는 PIC 0.7이상으로 나타났고 14개 marker를 조합시 부권부정율은 0.9999로 관찰되었다. 풍산개의 대립유전자 수는 2${\sim}$9개로서 expected heterozygosity와 PIC는 각각 0.262${\sim}$0.817 (평균 0.559), 0.222${\sim}$0.772 (평균 0.503)으로 나타났고 PEZ1, PEZ6, PEZ13의 marker는 PIC 0.7이상으로서 16개 marker를 조합시 부권부정율은 0.9991로 관찰되었다. 래버라도 리트리버의 대립유전자 수는 3${\sim}$5개로서 expected heterozygosity와 PIC는 각각 0.425${\sim}$0.808(평균 0.660), 0.354${\sim}$0.717(평균 0.563)으로 나타났고 PEZ8, PEZ12의 marker는 PIC 0.7이상으로 관찰되었으며 12개 marker를 조합시 부권부정율은 0.9968로 관찰되었다. 이상의 결과는 microsatellite DNA형에 의한 개의 친자감정 및 개체식별에 유용한 자료로 활용할 수 있을 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.784-788
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• 2006

Microsatellite polymorphism and the genetic relationship were estimated using genotype information of 305 horses from 11 microsatellite loci. The breeds include the indigenous Korean breeds, Korean native horse (102) and Jeju racing horse (56) together with Japan Hokkaido horse (5), Mongolian horse (19), Thoroughbred horse (108), Quarter horse (11) and Przewalskii horse (4). Allelic frequencies, the number of alleles per locus were estimated by direct counting from observed genotype, and genetic variability was computed using the CERVUX software and DISPAN. The number of alleles per locus varied from 6 (HMS6) to 18 (ASB17) with an average value of 10.45 in horse breeds. The expected total heterozygosity ($H_T$) and coefficient of gene differentiation ($G_{ST}$) ranged 0.764-0.921 (the average value was 0.830) and 0.102-0.266 (the average value was 0.180) in horse breeds, respectively. Four populations (Przewalskii horse, Japan Hokkaido horse, Quarter horse, Thoroughbred horse) showed lower heterozygosity than the average value (the average value was 0.710). The expected heterozygosity within breed ($H_S$) and mean no. of observed alleles ranged from $0.636{\pm}0.064$ (Japan Hokkaido horse) to $0.809{\pm}0.019$ (Mongolian horse), and from 2.73 (Przewalskii horse) to 8.27 (Korean native horse), respectively. The polymorphic information content (PIC) ranged from 0.490 (Przewalskii horse) to 0.761 (Mongolian horse) with an average value of 0.637 in horse breeds. The results showed three distinct clusters with high bootstrap support: the Korean native horse cluster (Korean native horse, Mongolian horse), the European cluster (Przewalskii horse, Thoroughbred horse), and other horse cluster (Jeju racing horse, Japan Hokkaido horse, and Quarter horse). A relatively high bootstrap value was observed for the Korean native horse cluster and European cluster (87%), and the Korean native horse and Mongolian horse (82%). Microsatellite polymorphism data were shown to be useful for estimating the genetic relationship between Korean native horse and other horse breeds, and also be applied for parentage testing in those horse breeds.