• Journal of Life Science
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• v.18 no.6
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• pp.902-906
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• 2008

In this study, we analyzed the microsatellite DNA poly-morphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 microsatellite loci were $0.6162{\sim}0.8746$ (mean 0.7587) and $0.5461{\sim}0.8512$ (mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (>0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parentage testing were noted significant differences compared with breeders. These results show basic information of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.

• Animal Bioscience
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• v.34 no.9
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• pp.1460-1465
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• 2021

Objective: The study aimed to evaluate the diversity of donkey populations by comparing with the diversity of Thoroughbred and Jeju Halla horses; identified breeding backgrounds can contribute to management and conservation of donkeys in South Korea. Methods: A total of 100 horse (50 Thoroughbreds and 50 Jeju Halla horses) and 79 donkeys samples were genotyped with 15 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3, and VHL20), to identify genetic diversity and relationships among horses and donkeys. Results: The observed number of alleles per locus ranged from 1 (ASB17, HMS1) to 14 (AHT5), with a mean value of 4.87, 8.00, and 5.87 in Thoroughbreds, Jeju Halla horses, and donkeys, respectively. Of the 15 markers, AHT4, AHT5, ASB23, CA425, HMS2, HMS3, HTG4, HTG10, and LEX3 loci had relatively high polymorphism information content (PIC) values (PIC>0.5) in these three populations. Mean levels of genetic variation were H_E = 0.6721 and H_O = 0.6600 in Thoroughbreds, H_E = 0.7898 and H_O = 0.7100 in Jeju Halla horses, and H_E = 0.5635 and H_O = 0.4861 in donkeys. Of the 15 loci in donkeys, three loci had negative inbreeding coefficients (FIS), with a moderate mean FIS (0.138). The FIS estimate for the HTG4 marker was highest (0.531) and HMS6 marker was lowest (-0.001). The total probability of exclusion value of 15 microsatellite loci was 0.9996 in donkeys. Conclusion: Genetic cluster analysis showed that the genetic relationship among 79 donkeys was generally consistent with pedigree records. Among the three breeds, donkeys and Thoroughbred horses formed clearly different groups, but the group of Jeju Halla horses overlapped with that of Thoroughbred horses, suggesting that the loci would be suitable for donkey parentage testing. Therefore, the results of this study are a valid tool for genetic study and conservation of donkeys.

• Journal of Life Science
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• v.19 no.2
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• pp.169-173
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• 2009

To assist in selection schemes we estimate the genetic diversity of the horse breeds. Genetic diversity at 13 microsatellite loci was compared in six horse breeds : Jeju Native Horse, American Quarter, Jeju Racing Horse, Mongolian Horse, Japanese Horse and Thoroughbred. All of the equine microsatellite used in this study were amplified and were polymorphic. The expected total heterozygosity over all the populations varied between 0.669 and 0.869 and the expected heterozygosity within population range from 0.569 to 0.219 in this study. The low coefficient of gene differentiation value showed that only 0.118 of the diversity was between horses breeds. The constructed dendrogram from the genetic distance matrix showed little differentiation between horse breeds using DISPAN program. The genetic distance using 13 microsatellites ranged between 0.137 and 0.414 for the six horse breeds. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in horse populations. The genetic diversity of the six horse breeds to each other closed to their geographical distribution. Suggesting that the loci would be suitable for horse breeds parentage testing. Therefore, Microsatellite marker seems to be very useful for clarifying the evolutionary relationships of closely related populations.