Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of Microsatellite DNA Polymorphisms for Pedigree Verification in Kyungju Dog(Dongkyung-i).

경주개(동경이)의 혈통확인을 위한 microsatellite DNA 다형성 분석

  • Lee, Eun-Woo (College of Veterinary Medicine, Kyungpook National University) ;
  • Choi, Seog-Gyu (Department of Planning Office School of Life-Health, Sorabol College) ;
  • Cho, Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we analyzed the microsatellite DNA poly-morphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 microsatellite loci were $0.6162{\sim}0.8746$ (mean 0.7587) and $0.5461{\sim}0.8512$ (mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (>0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parentage testing were noted significant differences compared with breeders. These results show basic information of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.

경주지방에서 사육 중인 경주개(동경이) 51두를 대상으로 8개의 microsatellite marker을 이용하여 DNA형을 분석한 결과 대립 유전자 수는 $4{\sim}12$개(평균 8.5개)로 검출되었으며 Expected heterozygosit와 PIC는 각각 $0.6162{\sim}0.8746$(평균 0.7587)와 $0.5461{\sim}0.8512$(평균 0.7167)으로 나타났고, PEZ3, PEZ6, PEZ12, FHC2054 marker는 PIC가 0.7이상으로 나타났다. 이들 marker는 향후 동경이의 개체식별 및 친자확인에 유용하게 쓰일 수 있다. 공시재료 51두 중 사육가에 의해 혈통이 알려진 5두를 대상으로 microsatellite marker를 이용하여 혈통을 분석한 결과 3두에서 현재 가계도와 일치하지 않았다. 따라서 앞으로 더 많은 연구를 통해서 동경이에 대한 체계적인 혈통 정립이 이루어져야 할 것이다.

Keywords

References

  1. Chae, Y. J., B. C. Lee and H. Lee. 1998. Paternity test in dogs by DNA analysis. J. Vet. Clin. 15, 274-278
  2. Cho, G. J. and B. W. Cho. 2006. Physical characteristics and microsatellite polymorphisms in Miryang native dogs. J. life Sci. 16, 626-631 https://doi.org/10.5352/JLS.2006.16.4.626
  3. Cho, G. J., B. W. Cho, S. K. Kim, K. W. Lee and Y. K. Kim. 2003. Analysis of microsatellite DNA polymorphism for parentage testing in dog breeds. J. Anim. Sci & Technol. (Kor) 45, 191-198 https://doi.org/10.5187/JAST.2003.45.2.191
  4. Cho, S. J., O. J. Kim, H. J. Park, S. G. Choi, M. G. Min, B. S. Kim, Y. S. Park and B. J. Cho. 2006. Historical and morphological investigation on Korean native Dongkyung-i dogs. Korean J. Companion Anim. Sci. 3, 45-53
  5. Denise, S., E. Johnston, J. Halverson, K. Marshall, D. Rosenfeld, S. Mckenna, T. Sharp and J. Edwards. 2004. Power of exclusion for parentage verification and probability of match for identity in American kennel club breeds using 17 canine microsatellite markers. Anim. Genet. 35, 14-17 https://doi.org/10.1046/j.1365-2052.2003.01074.x
  6. Girman, D. J., P. W. Kat, J. R. Ginsberg, M. G. Mills, M. Borner, V. Wilson, J. H. Fanshawe, C. Fitzgibbon, L. M. Lau and R. K. Wayne. 1993. Molecular genetic and morphological analyses of the African wild dog (Lycaon pictus). J. heredity 84, 450-459 https://doi.org/10.1093/oxfordjournals.jhered.a111371
  7. Ha, J. H., S. E. Lee, Y. B. Tak and J. B. Kim. 1998. The physical characteristics and blood proteins of Korean native dogs. Korean J. Anim. Sci. 40, 711-720
  8. Ha, J. H. and K. S. Kim. 1998. A review on the origin of Korean native dogs. Korean J. Anim. Sci. 40, 701-710
  9. Halverson, J. L. and J. W. Edwards. 2000. Microsatellite polymorphism in dog breeds-the AKC Parent Club study. pp. 19, 27th eds., ISAG Conf. Anim. Genet
  10. Ji, H. J., E. H. Kim, K. K. Lee, T. Y. Kang, J. M. Lee, H. D. Shin, L. H. Kim and Y. M. Yun. 2007. Beagle dogs parentage testing by using 22 ISAG microsatellite markers. Korean J. Vet. Res. 47, 457-460
  11. Jeong, H. W., K. S. Kim and J. H. Ha. 1997. Analysis of phylogenic relationships among the Asian 8 dog breeds (Canis familiaris) through Randomly Amplified Polymorphic DNA. Korean J. Genet. 19, 143-149
  12. Lee, S. L., H. S. Lee, W. B. Chung, R. B. Tak, H. C. Park, J. B. Kim and J. H. Ha. 1991. Selection of the useful DNA probe for the pedigree analysis of Korean dog breed, Sapsarees. Korean J. Genet. 13, 247-254
  13. Lee, S. U., S. Y. Hong and J. H. Ha. 1995. The pedigree analysis of Korean native dog, Sapsaree by mitochondrial and RFLP. Korean J. Genet. 17, 17-24
  14. Marshall, T. C., J. Slate, L. Kruuk and J. M. Pemberton. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol. 7, 639-655 https://doi.org/10.1046/j.1365-294x.1998.00374.x
  15. Tanabe, Y. and K. Ota. 1991. Biochemical-genetic relationships among asian, european dogs and the ancestary of the Japanese native dog. J. Anim. Breed Genet. 108, 455-478 https://doi.org/10.1111/j.1439-0388.1991.tb00207.x
  16. Tozaki, T., H. Kakoi, S. Mashima, K. I. Hirota, T. Hasegawa, N. Ishida, N. Miura, N. H. Choi-Miura and M. Tomita. 2001. Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci. J. Vet. Med. Sci. 63, 1191-1197 https://doi.org/10.1292/jvms.63.1191
  17. Vila, C., P. Savolainen, J. E. Maldonado, I. R. Amorim, J. E. Rice, R. L. Honeycutt, K. A. Crandall, J. Lundeberg and R. K. Wayne. 1997. Multiple and ancient origins of the domestic dog. Science 276, 1687-1689 https://doi.org/10.1126/science.276.5319.1687