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http://dx.doi.org/10.5352/JLS.2008.18.6.902

Analysis of Microsatellite DNA Polymorphisms for Pedigree Verification in Kyungju Dog(Dongkyung-i).  

Lee, Eun-Woo (College of Veterinary Medicine, Kyungpook National University)
Choi, Seog-Gyu (Department of Planning Office School of Life-Health, Sorabol College)
Cho, Gil-Jae (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Journal of Life Science / v.18, no.6, 2008 , pp. 902-906 More about this Journal
Abstract
In this study, we analyzed the microsatellite DNA poly-morphisms for pedigree verification in Kyungju dog (Dongkyung-i) which is one of the Korean breed dogs. A total of 51 Dongkyung-i samples were genotyped using 8 microsatellite markers. The number of alleles observed at single locus ranged from 4 to 12, with average number of alleles per locus of 8.5. The expected heterozygosity and polymorphic information contents (PIC) values of the 8 microsatellite loci were $0.6162{\sim}0.8746$ (mean 0.7587) and $0.5461{\sim}0.8512$ (mean 0.7167), respectively. Of the 8 markers, PEZ3, PEZ6, PEZ12 and FHC2054 loci had relatively high PIC values (>0.7) in Dongkyung-i. Pedigree verification of Dongkyung-i was analyzed based on alleles observed. The results of the parentage testing were noted significant differences compared with breeders. These results show basic information of conservation and research in Dongkyung-i, and further studies of genetic pedigree in Dongkyung-i will be needed.
Keywords
Dongkyung-i; microsatellite DNA polymorphisms; pedigree verification;
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