• Journal of Plant Biology
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• v.36 no.3
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• pp.285-292
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• 1993

Cellular compatibility in the self-parasitism of Cuscuta australis R. Brown was studied at the ultrastructural level. The front cells of the haustorium penetrated the host stems independently grew within the host tissues and transformed into elongate, filamentous hyphae. Each hyphal cells contained a large nucleus and dense cytoplasm with abundant cell organelles. Multilamellar structures were contained in the cytoplasm and cell walls of the penetrating hyphal cells. When the hyphal cells did not yet invade the host cells, the middle lamella and the fused cellulosic cell walls of the two partners at the host-parasite interface were preserved well. As the invasion of the parasitic hyphal cells progressed, however, the middle lamella was not found at the interface and the host cell walls and plasma membranes were partially broken down. A hyphal cell penetrated deeply into the host cell had a more darkly stained cytoplasm with numerous of cell organelles. In the host cells attacked by the hyphal cells the limiting membranes of plastids were broken down and several vesicles were arrayed near the cell walls. No plasmodesmatal connections between the host and parasite cell walls were found; however, half-plasmodesmata were observed frequently on the side of the hyphal cell walls. These results suggested that the compatibility response in the self-parasitism of Cuscuta was expressed by cell walls, not by plasmodesmata, between the host and the parasite cells.

• Korean Journal of Veterinary Service
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• v.17 no.1
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• pp.25-31
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• 1994

In order to investigate the internal parasitic infection, fecal samples were collected from weaning pig(n=123), porker(n=418) and sow(n=121) in 49 sawdust fermentative pigsty of Chonbuk district. The prevalence and identification of internal parasites were determined by the fecal examination using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows ; 1. The detection rate of parasite - eggs from 662 fecal samples was 86.6%. 2. The infection rate of parasite-egg 96.4% in porker, 76.9% in sow, 62.6% in weaning pig, in order. 3. In the concern of mired infection such as single, double triple and quadraple, the rate was 42.3%, 28.7%, 12.2% and 3.3%, respectively. 4. Ten kinds of the detected eggs were isolated from 662 fecal samples. They were classified as Balantidium coli (63.6%), Trichuris suis(24.8%), isospora spp.(23.5%), Oesoohangostomum spp.(17.8%), Ascaris suum(11.8%), Hyostronylus rubiddus (2.8%), strongyloides spp. (1.7%), Gnathostoma spp. (1.5%), Stephanurus dentatus(1.3%) and Metastrongylus spp. (0.7%), in order.

• Korean Journal of Veterinary Service
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• v.40 no.2
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• pp.95-99
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• 2017

Wild owls are widespread in Korea, even being common in urban areas due to the presence of hills or small mountains in and around the cities. This results in high levels of contact between owls and domestic animals. This study investigated the prevalence of avian hematozoan parasite infection in 2 common owl species in Chungbuk province of Republic of Korea for 3-year period: the Eurasian eagle owl (Bubo bubo) and the Brown hawk owl (Ninox scutulata). Peripheral blood smears taken from 56 wild owls were examined by microscopic examination and confirmed by molecular technique. Twenty (36%) of these samples tested positive for the Haemoproteus infection. The infection rate was higher in Brown hawk owls (50%) than in Eurasian eagle owls (33%). These results indicate that the wild owls may act as a reservoir for Haemoproteus infection in susceptible domestic birds.

• Korean Journal of Veterinary Service
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• v.34 no.3
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• pp.259-263
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• 2011

Wildlife has been recognized that has important role as reservoir hosts of disease affecting both people and livestocks. However, information intestinal helminths of Korean water deer were limited. We obtained 20 feces from Korean water deers that were transferred to veterinary hospital in Chungbuk National University from May 2010 to June 2011. Parasite ova were collected using saturated zinc sulfate floatation method. Parasite eggs were examined and measured using light microscope. The identification of parasitic eggs was done by morphological characters. The overall infection rate of parasite was 85% (n=17). The eggs were observed as being Nematodirus spp. (n=14), strongyles egg (n=11), Capillaria spp. (n=9), Trichuris spp. (n=7), Eimeria spp. (n=4), Ogmocotyle spp. (n=3), and Strongyloides (n=1). Mixed infection rate was observed as being single 15% (n=3), double 20% (n=3), triple 15% (n=3), quadruple 30% (n=6), and quintuple 5% (n=1). The Ogmocotyle spp. was first reported in Korea.

• Korean Journal of Veterinary Service
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• v.16 no.1
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• pp.82-89
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• 1993

In order to detection of the intestinal parasites, fecal samples were taken from broiler (n=262), laying hen(n=244), parent stock(n=207) and native stock(n=287) in Chonbuk province. The prevalence and identification of intestinal parasites were determined by the fecal examination using the floatation and /or sedimentation methods and microscopical examination, respectively. The results were obtained as follows : 1. The detection rate of parasite-eggs from 4 flocks(total=1,000) was 65.7%. 2. In the breed and type of breeding, infection rate of parasite-eggs was detected 84.0％ as native stock (floor breeding, 241 chicken), 79.7％ as parent stock (floor breeding, 165 chickens), 73.3% as broiler(floor breeding, 192 chicken) and 24.2% as laying hen(cage breeding, 59 chicken), in order. 3. In the concern of mixed infection such as single, double and triple, the rate was 55.1%, 8.7% and 1.9%, respectively. 4. Ten kinds of infective eggs were isolated from 657 fecal sample of 4 flock. They were classified 51.l% as Eimeria spp., 12.7% as Ascaridia galli, 5.1% as Capillaria spp., 4.1% as Strongyloides avium, 2.3% as Heterakis gallinarum, 0.5% as Hymenolepis spp., 0.3% as Railleina spp. and 0.2% as Syngamus spp., Trichostrongilus spp., or Choanoteania spp., single or in combination.

• Parasites, Hosts and Diseases
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• v.31 no.4
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• pp.375-378
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• 1993

The prevalence of Intestinal parasite Infections In Inhabitants at the reaches of the Hantan River, Chorwon-gun, Korea, was observed from August 12 to September 14, 1993. Of 465 people observed by cellophane thick smear and formalin-ether concentration method, 2 Ascots lumbricoides (unfertilized), 1 Trichwis Dichturn, 39 Clonorchis sinensis and 16 Metagonimus egg positive cases were found. After treatment, the Metogonimus egg positive cases passed out flukes of Metogonimus Mlyata type. Of 68 fish caught In the Hantan River, 14 (20.6%) were Infected with metacercanae of Metogontmn, while no metacercana of C. sinenis was found. At this area, soul-transmitted nematodes are very low, but clonorchlasis and metagonlmlasis are prevalent by modernate endemicity.

• Parasites, Hosts and Diseases
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• v.45 no.4
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• pp.247-253
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• 2007

Leishmania (L.) tropica is a causative agent of cutaneous leishmaniasis, and occasionally of visceral or viscerotropic leishmaniasis in humans. Murine models of Leishmania infection have been proven to be useful for elucidation of mechanisms for pathogenesis and immunity in leishmaniasis. The aim of this study was to establish a murine model for human viscerotropic leishmaniasis, and the growth pattern of L. tropica was studied in different tissues of BALB/c mice in order to find out whether the parasite visceralizes in this murine model. L. major was used as a control as this species is known to cause a progressive infection in BALB/c mice. L. tropica or L. major was injected into the footpad of mice, and thickness of footpad, parasite loads in different tissues, and the weight of the spleen and lymph node were determined at different intervals. Results showed that L. tropica visceralizes to the spleen and grows there while its growth is controlled in footpad tissues. Dissemination of L. tropica to visceral organs in BALB/c mice was similar to the growth patterns of this parasite in human viscerotropic leishmaniasis. The BALB/c model of L. tropica infection may be considered as a good experimental model for human diseases.

• Parasites, Hosts and Diseases
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• v.57 no.6
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• pp.581-585
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• 2019

Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens.

• Journal of Biomedical Engineering Research
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• v.40 no.5
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• pp.151-157
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• 2019

An acoustofluidic device using conductive liquid-based electrodes was developed for malaria parasite separation from white blood cells. In this device, the electrode channels filled with a conductive liquid were used to generate standing surface acoustic waves (SSAWs) in a fluidic channel, which can overcome the limitation of conventional patterned metal electrodes. Separation performance of the device was evaluated using fluorescent polystyrene particles with two different sizes (2 and $10{\mu}m$ diameters), which were successfully separated. In addition, a mixture of malaria parasites and white blood cells were also efficiently separated with high purity of ~98% in the CL-SSAW device at the flow rate of $12{\mu}l/min$.

• Parasites, Hosts and Diseases
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• v.57 no.4
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• pp.329-339
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• 2019

Indonesia and South Korea have become inseparable in various respects since the 2 countries established diplomatic relation in 1973. Indonesia is a tropical region that stretches across the equator, comprised of 5 main islands (Java, Kalimantan, Sumatra, Sulawesi, and Papua) and 4 archipelagoes (Riau, Bangka Belitung, Nusa Tenggara, and Maluku). As most population of Eastern Indonesia (Sulawesi, Papua and Nusa Tenggara & Maluku) live in poor areas, it is expected that there will be many parasites. Nevertheless, little is known about the status of parasites in Indonesia. This study examines the prevalences of malaria and lymphatic filaria, which are prevalent in Indonesia, as well as those of soil-transmitted-helminths (STH). As a result, the Plasmodium falciparum and P. vivax case loads are almost equal. The current prevalence of P. vivax is uniformly low (<5%) in all age groups and annual parasite incidence (API) showed decreasing tendency as 0.84 per 1,000 population in 2016. However, more than 65 million people still live in malaria epidemic regions. Lymphatic filariasis remains an important public health problem and 236 cities were classified as endemic areas in 514 cities/districts in 2017. It is difficult to ascertain the current prevalence rate of STH in Indonesia, although West Sumba and Southwest Sumba in East Nusa Tenggara reported prevalence rate of more than 20%. The study also considers the (sero) prevalences of other parasites identified in Indonesia. This report should be useful not only to parasitologists but also to travelers and people with business in Indonesia.