• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1868-1874
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• 2007

We have developed a robotic system for an automated parallel cell cultivation process that enables screening of induction parameters for the soluble expression of recombinant protein. The system is designed for parallelized and simultaneous cultivation of up to 24 different types of cells or a single type of cell at 24 different conditions. Twenty-four culture vessels of about 200 ml are arranged in four columns${\times}$six rows. The system is equipped with four independent thermostated waterbaths, each of which accommodates six culture vessels. A two-channel liquid handler is attached in order to distribute medium from the reservoir to the culture vessels, to transfer seed or other reagents, and to take an aliquot from the growing cells. Cells in each vessel are agitated and aerated by sparging filtered air. We tested the system by growing Escherichia coli BL21(DE3) cells harboring a plasmid for a model protein, and used it in optimizing protein expression conditions by varying the induction temperature and the inducer concentration. The results revealed the usefulness of our custom-made cell cultivation robot in screening optimal conditions for the expression of soluble proteins.

• Journal of Microbiology and Biotechnology
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• v.27 no.5
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• pp.947-955
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• 2017

Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to $180{\mu}m$, high volumetric production of herbicidin A was obtained with OUR values >137mg $O_2/l{\cdot}h$. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of $100-200{\mu}m$ length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.6
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• pp.357-362
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• 2015

A novel $TiO_2$-Coconut Shell Powder Composite (TCSPS), prepared by the controlled sol-gel method with subsequent heat treatment, was evaluated as an innovative photocatalytic absorbent for the removal of methylene blue. Optimal preparation conditions of TCSPC were obtained by a response surface methodology and a central composite design model. As compared with the results obtained from one-factor-at-a-time experiments, the values were approximated to the nearest condition of these values and the following experimental parameters were set as the optimum : $600^{\circ}C$ calcination temperature and 20 g of coconut shell powder loading amount.

• Environmental Engineering Research
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• v.21 no.2
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• pp.196-202
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• 2016

Membrane bioreactor (MBR) technology has previously been used by water industry to treat high salinity wastewater. In this study, an anoxic-oxic biofilm-membrane bioreactor (AOB-MBR) system has been developed to treat mustard tuber wastewater of 10% salinity (calculated as NaCl). To figure out the effects of operating conditions of the AOB-MBR on membrane fouling rate ($K_V$), response surface methodology was used to evaluate the interaction effect of the three key operational parameters, namely time interval for pump (t), aeration intensity ($U_{Gr}$) and transmembrane pressure (TMP). The optimal condition for lowest membrane fouling rate ($K_V$) was obtained: time interval was 4.0 min, aeration intensity was $14.6 m^3/(m^2{\cdot}h)$ and transmembrane pressure was 19.0 kPa. And under this condition, the treatment efficiency with different influent loads, i.e. 1.0, 1.9 and $3.3kgCODm^{-3}d^{-1}$ was researched. When the reactor influent load was less than $1.9kgCODm^{-3}d^{-1}$, the effluent could meet the third discharge standard of "Integrated Wastewater Discharge Standard". This study suggests that the model fitted by response surface methodology can predict accurately membrane fouling rate within the specified design space. And it is feasible to apply the AOB-MBR in the pickled mustard tuber factory, achieving satisfying effluent quality.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.11
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• pp.2525-2531
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• 2012

Al-Cu alloy has been used as a circuit material for its low resistance and ease to process for long years at CMOS technology. However, basically metal is very susceptible to corrosion and which has been a long pending trouble in various fields using metal. The defect causes the reliability concerns, so improved methods are necessary to reduce the defect. In the various corrosion parameters, PR strip process conditions after metal etch and optimal cleaning solutions are controllable and increase the process margin to prevent the metal corrosion. This study proposes that chlorine residue after metal etch as the source of metal corrosion, and charges should be removed by optimizing PR strip process condition and cleaning condition.

• Journal of Power Electronics
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• v.18 no.2
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• pp.547-557
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• 2018

This work proposes a control method of frequency stabilization for grid integration of large-scale wind farms via the voltage source converter-based high-voltage direct current (VSC-HVDC) technology. First, the topology of grid integration of a large-scale wind farm via the VSC-HVDC link is provided, and simple control strategies for wind turbines, wind farm side VSC (WFVSC), and grid side VSC are presented. Second, a mathematical model between the phase angle of WFVSC and the frequency of the wind farm is established. The control principle of the large-scale wind power integrated system is analyzed in theory in accordance with the mathematical model. Third, frequency and AC voltage controllers of WFVSC are designed based on the mathematical model of the relationships between the phase angle of WFVSC and the frequency of the wind farm, and between the modulation index of WFVSC and the voltage of the wind farm. Corresponding controller structures are established by deriving a transfer function, and an optimization method for selecting the parameters of the frequency controller is presented. Finally, a case study is performed under different operating conditions by using the DIgSILENT/PowerFactory software. Results show that the proposed control method has good performance in the frequency stabilization of the large-scale wind power integrated system via the VSC-HVDC technology.

• 한국태양에너지학회:학술대회논문집
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• 2009.04a
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• pp.184-189
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• 2009

In order to improve the operation of energy systems, it is necessary for the urban communities to have reliable optimization routines, both computerized and manual, implemented in their organizations. However, before a production plan for the energy system units can be constructed, a prediction of the energy systems first needs to be determined. So, several methodologies have been proposed for energy demand prediction, but due to uncertainties in urban community, many of them will fail in practice. The main topic of this paper has been the development of a method for energy demand prediction at urban community. Energy demand prediction is important input parameters to plan for the energy planing. This paper presents a energy demand prediction method which estimates heat and electricity for various building categories. The method has been based on artificial neural networks(ANN). The advantage of ANN with respect to the other method is their ability of modeling a multivariable problem given by the complex relationships between the variables. Also, the ANN can extract the relationships among these variables by means of learning with training data. In this paper, the ANN have been applied in oder to correlate weather conditions, calendar data, schedules, etc. Space heating, cooling, hot water and HVAC electricity can be predicted using this method. This method can produce 10% of errors hourly load profile from individual building to urban community.

• Journal of Korea Water Resources Association
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• v.36 no.5
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• pp.851-861
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• 2003

Snow accumulation and snow melt was simulated and included in the computation of the watershed runoff for Soyanggang Dam and Chungju Dam. A modified Tank Model was used for the simulation, which has three serial tanks and a pulse response function. The model parameters were estimated through the global optimization method of Shuffled Complex Evolution-University of Arizona (SCE-UA). A watershed was divided into four zones of elevation. The temperature decrease of the zones was a rate of -0.6$^{\circ}C$/100m. Almost all precipitation from December to February become accumulated as snow, and then the snow melts and runs off from March to April. The average runoff with snow melt was greater than the average runoff without snow melt during the period from March to April. The improved amount from snow melt simulation was about one fifth of the observed one for Soyanggang Dam. The increased amount for Chungju Dam was about one fourth of the observed average runoff during the same period. Although the watershed runoff was simulated including snow melt, it was less than the observed one for both of the dams.

• Korean Journal of Optics and Photonics
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• v.20 no.3
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• pp.148-155
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• 2009

In this paper, we analysed what effect the design variables, such as refractive index, central thickness and radius of curvature, had on the first order properties and image quality of optical systems when temperature changed. The optical parameters were varied at each temperature, then the coupling and ruler methods were used to design an athermalized lens for a phone camera. This concept was first used to design the lens for a 1/3.2" 5M phone camera. The designed lens satisfies all the specifications for a phone camera, and the variations of the back focal length(${\Delta}BFL$) are reduced to $10{\mu}m$ for a temperature range of $-10^{\circ}C$ to $+60^{\circ}C$. Also, the TTL of 5.5 mm results in a compact system. All design concepts and results discussed in this paper are expected to be useful in development for the phone and CCTV camera.

• 제어로봇시스템학회:학술대회논문집
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• 1996.10b
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• pp.1476-1479
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• 1996

The ring rolling process is one of the best known ring production method. The present model based control system was designed for rings with rectangle cross-section yet. An Adaptive Fuzzy Control for Closed-Die Ring-Rolling was developed in order to enhance the flexibility of the radial-axial ring rolling machine and to produce the rings with highly complex cross-section profile, roller bearing rings. A fuzzy method was implemented because of its simple application and to utilize the known process knowledge. The quality of the control system was estimated by die filling grad, which is strong dependent on the rising time of the controller. The rolling process parameters were also varied to determine their influence on filling of the ring profile. Die filling met the requirement of the industry.