• 한국산림과학회지
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• 제94권2호통권159호
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• pp.67-72
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• 2005

The objectives of this study were to identify the period of initiation of floral primordia in black locust (Robinia pseudoacacia L.) and subsequent development of floral buds until following spring. Four mature trees of black locust located in Suwon, Korea were selected. Bud samples were collected from the current-year shoots, starting from mid June to July every week, from August to October and from February to April every month. The buds were fixed in FAA solution, dehydrated, and imbedded in paraffin for microscopic observation. Buds collected on June 16, and 23, 1997, contained primitive primordia that might be interpreted as early floral primordia. By June 30, a bud showed a positive indication of inflorescence primordium with a well-formed shoot apex. All the inflorescence primordia observed throughout the collection periods were always associated with unique hairy appendages around the primordium and enclosed within a sclerenchymatous chamber. By July 7 and 15, a floral apex had early bud scales. By July 22, primitive inflorescence developed into visible arrangement of individual floral primordial By July 29, the inflorescence developed into whirl arrangement of individual floral primordia in a transverse section, but showed little further development until October 15. The inflorescence primordia seemed to over-winter at this stage. Buds collected from February 15 and March 24 the following year also showed no further development of inflorescence primordia. By April 7 the inflorescence started to show further development with elongated axis. At this time individual flowers were easily recognized.

• 대한세포병리학회지
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• 제5권2호
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• pp.79-89
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• 1994

This study was performed in order to evaluate the accuracy and the usefulness of the cytology of crush preparation in central nervous system (CNS) lesions. Forty four intraoperative biopsies were performed at the time of craniotomy including 34 benign and 10 malignant lesions. Crush preparations were prepared from tiny tissue fragments of craniotomy products. All cases were stained with toluidine blue. Intraoperative diagnoses made on cytologic examination were compared with the final paraffin section diagnoses. Comparison between the results of the cytologic and histologic findings revealed an overall diagnostic accuracy of 88.6%. This study attests to the diagnostic accuracy of cytologic examination in CNS lesions. The detailed cytologic features are described and important criteria for the cytodiagnosis of CNS lesions are discussed. This review leads us to think that cytologic examination by crush preparation in CNS lesions is considered as a simple, safe and highly diagnostically accurate method.

• 한국산림과학회지
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• 제7권1호
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• pp.3-7
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• 1968

본(本) 대학(大學) 연습림(演習林)에서 생육(生育)한 32년생(年生) 잎갈나무와 27년생(年生) 가래나무를 이용(利用)하여 시험(試驗)한 목재(木材)의 구조단면별(構造斷面別) 흡수성(吸收性) 및 수침시간(水浸時間)에 의(依)한 단면별(斷面別) 흡수성(吸收性)을 요약(要約)하면 다음과 같다. (1) 본시험(本試驗)에 사용(使用)한 시편(試片)($30{\times}30{\times}100cm$)을 각(各) 단면별(斷面別)로 흡수면(吸水面)만 남기고 paraffin과 vaseline과의 등량혼합물(等量混合物)로 방수(防水)하여 $25{\pm}1^{\circ}c$의 청수(淸水)에 8.5일간(日間) 침지(浸漬) 0.5일(일)(12시간)마다 중량(重量)을 측정(測定)하여 흡수성(吸水性) 조사(調査)하였다. (2) 목구면(木口面)에서의 흡수성(吸水性)은 구목면(柾目面)이나 구목면(柾目面)의 종단면(縱斷面)에서의 흡수성(吸水性)보다 대단(大端)히 크며 구목면(柾目面)과 판목면(板目面)에 있어서는 차이(差異)가 거의 없다. (3) 가래나무의 구목면(柾目面)을 제외(除外)하고는 비중(比重)이 비교적(比較的) 적은 가래나무가 비중(比重)이 보다 큰 잎갈나무보다 각(各) 단면(斷面)에서의 흡수성(吸水性)이 크다. (4) 침지시간(浸漬時間) 경과(經過)함에 따라 흡수성(吸水性)이 증가(增加)된다. 또한 침지초기(浸漬初期)에는 흡수성(吸水性)이 대단(大端)히 크나 시간(時間)이 경과(經過)함에 따라 그 정도(程度)는 점차(漸次) 감소(減少)된다. 흡수율(吸水率) 약(約) 반(半) 이상(以上)은 대게 침지(浸漬) 48시간(時間) 전후(前後)의 초기(初期)에 이루어진다. (5) 목구면(木口面)에서는 침지시간(浸漬時間)이 경과(經過)함에 따라 잎갈나무와 가래나무의 흡수성(吸水性) 점차(漸次) 큰 차이(差異)를 나타내며 구목면(柾目面)에서는 초기(初期)의 다소(多少)의 차이(差異)가 있으나 시간(時間)이 경과(經過)함에 따라 아주 일치(一致)한다. (6) 침지시간(浸漬時間)과 흡수량(吸水量)사이에는 다음과 같은 실험식(實驗式)이 성립(成立)한다.

• 대한수의학회지
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• 제33권2호
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• pp.269-275
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• 1993

토끼의 바이러스성 간염, 소위 토끼 출혈병의 원인체에 대한 각종 세포주들에 바이러스증식을 유도하고 한편 실험적 감염예에 대한 면역형광항체법과 immunoperoxidase 방법에 의한 원인체의 조직내에 분포상황을 조사하기 위하여 감염된 토끼를 폐사직후 부검하여 간장, 비장, 신장, 폐 및 뇌조직을 절제하여 동결절편하거나 또는 포르말린고정 파라핀 포매 절편을 $5{\sim}7{\mu}m$로 작제하여 면역반응에 공시하였다. 공시된 각종 세포주에 대한 원인체의 세포배양성은 인정되지 않았으며 면역조직화학적방법을 이용한 면역반응에서는 간장에서 강한 양성반응을 보였으며, 비장과 신장에서는 소수예에서 백색수주변 대식세포와 신사구체에서 양성반응을 각각 나타내었다. 그러나 기타 장기에서는 특이한 양성반응이 인정되지 않았다. ABC immunoperoxidase 방법을 이용한 간장의 포르말린고정 파라핀포매 절편에서 portal triad를 중심으로한 소엽주변부 간세포에서 강한 양성반응을 나타내었으며, 이들 양성반응은 간세포 및 동양혈관세포의 세포질내에 미세과립상으로 미만성 및 세포질주변성으로 관찰되었다. 그리고 감염세포와 비감염세포와의 구별이 명확히 인정되었고 양성반응을 보이는 부위는 H-E 염색상 변성괴사된 간세포에 일치되었다. 이상의 결과에서 본 질병의 표적기관은 간장이며 포르말린고정 파라핀포매 간조직의 immunoperoxidase 방법에 의한 면역조직화학적방법이 본병 진단에 크게 활용되리라 본다.

• 한국양식학회지
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• 제10권1호
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• pp.55-61
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• 1997

이 실험은 능성어의 친어관리와 종묘생산을 위한 연구의 일환으로 HCG 처리에 의한 난의 성숙과 배란유도를 알아보고자 행하였다. 제주대학교 해양연구소와 한라수산에서 1996년 6월에서 8월에 걸쳐 300-400 g, 1,500-2,000 g, 2,500-4,000 g 되는 능성어를 대상으로 하여 HCG를 500-1,000 IU/kg 체중농도로 2-3회 처리하였다. 이들의 생식소 발달은 일반적인 paraffin 절편법에 의하여 haematoxylin과 eosin으로 비교염색하여 조사하였다. 체중 300-400 g, 1,500-2,000 g, 2,500-3,200 g 되는 능성어에 HCG를 500-1,000 IU/kg 체중농도로 2-3회 처리한 결과 생식소는 난원세포군, 20~$30\mu$m 내외의 어린 난모세포를 가지고 있었다. 그러나 체중 3,400-4,000 g 되는 능성어에 HCG 1,000 IU/kg 농도로 2회 처리한 결과 난경 ~$800\mu$m되는 건강한 난과 퇴행란이 배란되었다. 체중 4,000 g 개체에서 배란된 난수는 4,252개 였으며, 이 중에서 건강한 난은 20.8%, 퇴행란은 79.2%였다. 그리고 생식소내에는 배란 흔적과 함께 난경 ~$50\mu$m전후의 주변인기 난모세포, 20~$30\mu$m의 어린 난모세포들이 차지하고 있다.

• 대한본초학회지
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• 제23권2호
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• pp.25-31
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• 2008

Objectives : In order to distinguish morphological characteristics of trunk bark and root bark of Ulmus davidiana var. japonica (Rehder) Nakai and the trunk bark and root bark of Hemiptelea davidii Planchon were sampled and compared in terms of their external and internal features with flour states according to their medical use, through microscopic examination. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and the flour of it was mounted by the liquid made by the same ratio of each of glycerin, acetic acid, and water, and then observed and photographed by olymphus-BHT. Results : 1. Internal Features 1) A large parenchymatous cell was observed in the phloem of the slice of both trunk bark and root bark of Ulmi Cortex, However, both of the trunk bark and root bark of Hemipteleae Cortex did not have parenchymatous cell in the phloem; instead, stone cells including much square crystal of calcium oxalate were distributed around fiber bundle, and the parenchymatous cell included much druse crystal of calcium oxalate. 2) In both the Ulmi Cortex and Hemipteleae Cortex, rhytidome was observed in trunk bark, but not in root bark, but in the parenchymatous cell of the root bark of the Ulmi Cortex contained starch grain. 2. Flour States 1) In the flour of root bark of the Ulmi Cortex, a large parenchymatous cell was observed. However, in the flour of trunk bark and root bark of Hemipteleae Cortex, no parenchymatous eel was found; instead, stone cell including square crystal of calcium oxalate and druse crystal of calcium oxalate were observed. 2) There was no remarkable difference between the trunk bark and root bark of Hemipteleae Cortex. However, starch grain was contained in the parenchymatous cell of the root bark of Ulmi Cortex but not in the trunk bark of it. Conclusions : There were some morphological differences in external, internal, and flour parts of Ulmi Cortex and Hemipteleae Cortex. In particular, there was a morphological difference in flour states between the trunk bark and root bark of Ulmi Cortex, it is possible to use microscope to distinguish their flour states.

• 한국수정란이식학회지
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• 제23권3호
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• pp.183-187
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• 2008

This study was conducted to analyze the expression pattern of inhibitor of DNA binding proteins (Id)1 and Id2 mRNA on folliculogenesis in rat ovary. The ovaries were obtained from 27 days old Sprague-Dawley rat, fixed, dehydrated, and paraffin embedded. For in situ hybridization, anti-sense and sense Idl and Id2 cRNA probes were prepared and applied to the ovarian section. The ovarian sections were coated with NTB-2 emulsion. After that, the slides were developed and counterstained with hematoxylin and eosin staining. In oocytes, the hybridizational signals of Id1 mRNA were strong in primordial and primary follicles, however, there were no signals in that of atretic or preovulatory follicles. The Id2 mRNA signals were also strong in the oocytes of primordial, primary and secondary follicles. Interestingly, the Id2 mRNA was expressed specifically granulosa cells, but nor in oocyte or theca cells in dominant and preovulatory follicles. Based on these results, Id1 and Id2 mRNA was expressed specifically at follicle stages and follicular tissue and might be closely related with follicle development.

• 대한본초학회지
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• 제22권2호
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• pp.103-108
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• 2007

Objectives : A morphological classification among Pinelliae Tuber, Typhonii Flagelliformis Rhizoma and Arisaematis Rhizoma was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Pinelliae Tuber is like a ball with a $1{\sim}1.5cm$ diameter, with a concave apex and pockmarked root trace around. 2. Typhonii Flagelliformis Rhizoma is somewhat oval or like a cone, with a $4{\sim}8mm$ diameter, a $7{\sim}15mm$ height, a projected round apex, and a pin-pointed back. 3. Arisaematis Rhizoma is somewhat flat round with a $2{\sim}2.5cm$ diameter, a little than Pinelliae Tuber, and its apex has incomplete lobopodium and root trace. 4. Fundamental tissues of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma, and Arisaematis Rhizoma have starch grains and mucilage cells, and their mucilage cells include the raphides of calcium oxalate. Conclusion : Internal forms of Pinelliae Tuber, Typhonii Flagelliformis Rhizoma, and Arisaematis Rhizoma are difficult to distinguish from one another, but Pinelliae Tuber and Arisaematis Rhizoma can be distinguished by their external forms and sizes, and Pinelliae Tuber and Typhonii Flagelliformis Rhizoma were distinguished by the forms of apex.

• 대한본초학회지
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• 제20권4호
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• pp.95-101
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• 2005

Objectives : In order to distinguish Cnidii Rhizoma and Ligustici Chuanxiong Rhizoma (cultivated in Korea and China), their external and internal states were observed via microscopic examination. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : Cnidii Rhizoma, Ligustici Chuanxiong Rhizoma (from Korea), and Ligustici Chuanxiong Rhizoma (from China) all demonstrated similar patterns as following : 1. external form 1) showing irregular nodular form with $2{\sim}7cm$ diameter. 2) having several trochites in parallel, and nodulose root trace on surface. 3) in addition to yellow-white or yellow-gray intersections, yellow-brown oil sacs were distributed, with cambium resembling a form of annulus. 2. internal form 1) its root and rhizome is composed of 10 layers of cork, with narrow cortical layers. 2) its phloem is wide, and oval light yellow-brown oil sacs are distributed around. The closer to the cambium, the smaller it is. 3) cambium is a form of a wave-like ring or an irregular polygon. 4) xylem and vessel are rare, most of which are arranged in a 'V' format, and sometimes xylem fiber is found. 5) pith is composed of parenchymatous cell, and parenchyma is full of starch grains. Conclusions : In conclusion, C officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) have rhizomes in a similar form with no special difference. Therefore, it is concluded that C. officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) are categorized into the same species.

• 대한본초학회지
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• 제21권2호
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• pp.181-188
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• 2006

Objectives : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix imported from China are circulated in Korea. Morphological identification was attempted by comparing external and internal forms of these materials. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Southern type of Osterici Radix has short rhizome and many small and long roots, while northern type of it is mostly composed of rhizomes with little small reds and sometimes looks like bamboo because of the extention of the parts between joints. 2. Parenchymal cell of southern type of Osterici Radix is filled with starch grain, while that of nothern type includes less starch grain with lamination crossed on its cell wall. 3. Surface of root and rhizome of wild Osterici Radix has some marks of fibrous root raised in the form of nodule, but cultivated Osterici Radix does not have this kind of marks. 4. Notopterigii Rhizoma et Radix is mostly composed of rhizomes, and its form is similar to that of a silkworm or joints of bamboo. Parenchymal cell is filled with starch grains, and lamination is crossed on its cell wall but not salient. Conclusion : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix circulated in Korea can be easily identified because of their differences in their external and internal forms.