Browse > Article

Inhibitor of DNA Binding Protein (Id)1 and Id2 mRNA Expression on Folliculogenesis in Rat Ovary  

Hwang, Seong-Soo (National Institute of Animal Science, RDA)
Lee, Pyung-Hee (National Institute of Animal Science, RDA)
Ko, Yeoung-Gyu (National Institute of Animal Science, RDA)
Yang, Byoung-Chul (National Institute of Animal Science, RDA)
Seong, Hwan-Hoo (National Institute of Animal Science, RDA)
Min, Kwan-Sik (Graduate School of Bio-& Information Technology, Hankyong University)
Yoon, Jong-Taek (Dept. of Animal Life & Resources, Hankyong University)
Publication Information
Journal of Embryo Transfer / v.23, no.3, 2008 , pp. 183-187 More about this Journal
Abstract
This study was conducted to analyze the expression pattern of inhibitor of DNA binding proteins (Id)1 and Id2 mRNA on folliculogenesis in rat ovary. The ovaries were obtained from 27 days old Sprague-Dawley rat, fixed, dehydrated, and paraffin embedded. For in situ hybridization, anti-sense and sense Idl and Id2 cRNA probes were prepared and applied to the ovarian section. The ovarian sections were coated with NTB-2 emulsion. After that, the slides were developed and counterstained with hematoxylin and eosin staining. In oocytes, the hybridizational signals of Id1 mRNA were strong in primordial and primary follicles, however, there were no signals in that of atretic or preovulatory follicles. The Id2 mRNA signals were also strong in the oocytes of primordial, primary and secondary follicles. Interestingly, the Id2 mRNA was expressed specifically granulosa cells, but nor in oocyte or theca cells in dominant and preovulatory follicles. Based on these results, Id1 and Id2 mRNA was expressed specifically at follicle stages and follicular tissue and might be closely related with follicle development.
Keywords
inhibitor of DNA binding protein (Id)l and Id2; ovary; folliculogenesis; rat; in situ hybridization;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Benezra R, Rafii S and Lyden D. 2001. The Id proteins and angiogenesis. Oncogene 20:8334-41   DOI
2 Hollnagel A, Oehlmann V, Heymer J, Rüther U and Nordheim A. 1999. Id genes are direct targets of bone morphogenetic protein induction in embryonic stem cells. J. Biol. Chem. 274:19838-19845   DOI
3 Im GS, Seo JS, Hwang IS, Kim DH, Kim SW, Yang BC, Yang BS, Lai L and Prather RS. 2006. Development and apoptosis of pre-implantation porcine nuclear transfer embryos activated with different combination of chemicals. Mol. Reprod. Dev. 73:1094-1101   DOI   ScienceOn
4 Lasorella A, Uo T and Iavarone A. 2001. Id proteins at the crossroad of development and cancer. Oncogene 20:8326-8333   DOI
5 Lee HJ, Hwang SS and Yoon JT. 2007. Effects of bovine somatotropin (bST) administration combined with controlled internal drug release (CIDR) on embryo quality and pregnancy of Hanwoo (Korean native beef cattle) during commercial embryo transfer program. Asian-Aust. J. Anim. Sci. 20:194-199
6 Massari ME and Murre C. 2000. Helix-loop-helix proteins: regulators of transcription in eucaryotic organisms. Mol. Cell Biol. 20:429-440   DOI
7 McGee EA and Hsueh AJ. 2000. Initial and cyclic recruitment of ovarian follicles. Endocr. Rev. 21(2):200-14   DOI   ScienceOn
8 Shimasaki S, Moore RK, Otsuka F and Erickson GF. 2004. The bone morphogenetic protein system in mammalian reproduction. Endocr. Rev. 25:72-101   DOI   ScienceOn
9 Springhorn JP, Singh K, Kelly RA and Smith TW. 1994. Posttranscriptional regulation of Id1 activity in cardiac muscle. Alternative splicing of novel Id1 transcript permits homodimerization. J. Biol. Chem. 269:5132-5136
10 황성수, 고응규, 임현주, 성환후, 윤종택, 민관식, 2007. 랫드 난소에서 난포발달에 따른 Id3 mRNA의 발현, 한국동물번식학회지 31:181-186   과학기술학회마을
11 Son DS, Choe CY, Cho SR, Choi SH, Kim HJ, Lee JI and Kim IH. 2008. Timed artificial insemination or embryo transfer using CIDR, estradiol benzoate, and prostaglandin F2 for the rebreeding of Korean native donor cettle. J. Emb. Trans. 23:81-86
12 Fortune JE. 2003. The early stages of follicular development: activation of primordial follicles and growth of preantral follicles. Anim. Reprod. Sci. 78:135-163   DOI   ScienceOn
13 Howe HL, Wingo PA, Thun MJ, Ries LA, Rosenberg HM, Feigal EG and Edwards BK. 2001. Annual report to the nation on the status of cancer (1973 through 1998), featuring cancers with recent increasing trends. J. Natl. Cancer Inst. 93: 824-842   DOI   ScienceOn
14 Norton JD. 2000. ID helix-loop-helix proteins in cell growth, differentiation and tumorigenesis. J. Cell Sci. 113:3897-3905   PUBMED
15 Nakashima K, Takizawa T, Ochiai W, Yanagisawa M, Hisatsune T, Nakafuku M, Miyazono K, Kishimoto T, Kageyama R and Taga T. 2001. BMP2-mediated alteration in the developmental pathway of fetal mouse brain cells from neurogenesis to astrocytogenesis. Proc. Natl. Acad. Sci. U.S.A. 98:5868-5873
16 Johnson AL, Haugen MJ and Woods DC. 2008. Role for inhibitor of differentiation/deoxyribonucleic acid-binding (Id) proteins in granulosa cell differentiation. Endocrinology 149: 3187-3195   DOI   ScienceOn
17 Hwang SS, Choi EJ, You SK, Choi YJ, Min KS and Yoon JT. 2006. Development of bovine nuclear transfer embryos using life-span extended donor cells transfected with foreign gene. Asian-Aust. J. Anim. Sci. 19:1574-1579
18 Yang Y, Wang HC, Sun XH. 2008. Id1 induces apoptosis through inhibition of ROR gammat expression. BMC Immunol. 9: 20   DOI   ScienceOn
19 Miyazono K and Miyazawa K. 2002. Id: A Target of BMP Signaling. Sci. STKE. 151. pe40
20 Park JK, Lee YK, Lee PY, Chung HJ, Kim SW, Lee HG, Seo MK, Han JH, Park CG, Kim HT, Kim YK, Min KS, Kim JH, Lee HT and Chang WK. 2006. Recombinant human erythropoietin produced in milk of transgenic pigs. J. Biotech. 122:362-371   DOI   ScienceOn
21 Matsui M, Sonntag B, Hwang SS, Byerly T, Hourvitz A, Adashi EY, Shimasaki S and Erickson GF. 2004. Pregnancyassociated plasma protein-a production in rat granulosa cells: stimulation by follicle-stimulating hormone and inhibition by the oocyte-derived bone morphogenetic protein-15. Endocrinology 145:3686-3695   DOI   ScienceOn
22 Yang BC, Im GS, Kim DH, Yang BS, Oh HJ, Park HS, Seong HH, Kim SW, Ka HH and Lee CK. 2008. Development of vitrified-thawed bovine oocytes after in vitro fertilization and somatic cell nuclear transfer. Anim. Reprod. Sci. 103: 25-37   DOI   ScienceOn
23 Yokota Y. 2001. Id and development. Oncogene. 20:8290-8298   DOI
24 Hourvitz A, Kuwahara A, Hennebold JD, Tavares AB, Negishi H, Lee TH, Erickson GF and Adashi EY. 2002. The regulated expression of the pregnancy-associated plasma protein- A in the rodent ovary: a proposed role in the development of dominant follicles and of corpora lutea. Endocrinology 143:1833-1844   DOI   ScienceOn
25 Knight PG and Glister C. 2006. TGF-beta superfamily members and ovarian follicle development. Reproduction 132: 191-206   DOI   ScienceOn
26 Matsumura ME, Lobe DR and McNamara CA. 2002. Contribution of the helix-loop-helix factor Id2 to regulation of vascular smooth muscle cell proliferation. J. Biol. Chem. 277: 7293-7297   DOI   ScienceOn
27 Gougeon A. 1996. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocr. Rev. 17: 121-155   DOI   PUBMED
28 Erickson GF and Shimasaki S. 2000. The role of the oocyte in folliculogenesis. Trends Endocrinol. Metab. 11:193-198   DOI   ScienceOn