• Current Research on Agriculture and Life Sciences
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• v.4
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• pp.55-64
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• 1986

Polyphenol oxidase in japanese pear (Pyrus communis var. mansamkil) was isolated, partially purified and its some properties were investigated. Polyacrylamide disc gel electrophoresis indicated two bands with polyphenol oxidase activity in the extract from acetone dry powder of par flesh. These two polyphenol oxidases (PPO A and PPO B) were purified through acetone precipitation and diethylaminoethyl cellulose column chromatography. PPO A and B were purified 7.8 fold and 8.7 fold by the present procedure, respectively. The Rm values of partially purified PPO A and B were estimated to be 0.58 and 0.68, respectively. The optimum temp, and pH of PPO A activity were $33^{\circ}C$ and pH 7.0, while those of PPO B were $30^{\circ}C$ and pH 4.2, respectively. Two PPO were unstable over the temperature of $60^{\circ}C$. The substrate specificity of pear PPO showed high affinity toward o-diphenolic compounds, especially catechol in PPO A and chlorogenic acid in PPO B, but inactive toward m-diphenol, p-diphenol and monophenols. PPO A showed affinity toward the trihydroxyphenolic compound. $Zn^{{+}{+}}$ activated the PPO A activity but $Fe^{{+}{+}}$ inhibited PPO B activity, while $Fe^{{+}{+}}$ and $Zn^{{+}{+}}$ activated the PPO B activity, while $Fe^{{+}{+}}$ and $Zn^{{+}{+}}$ activated the PPO B activity but $K^+$, $Mg^{{+}{+}}$, $Ca^{{+}{+}}$ and $Hg^{{+}{+}}$ inhibited at 10mM concentration. $Cu^{{+}{+}}$ activated the enzyme action at low concentrations but inhibited at high concentration. Inhibition studies indicated that L-ascorbic acid, L-cysteine and thiourea were most potent. The Km values of PPO A and PPO B for catechol were 20mM and 14.3mM, respectively.

• Korean Journal of Plant Resources
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• v.8 no.2
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• pp.195-199
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• 1995

This study was conducted to investigate the Net photosynthesis and respiration rates among the varieties of Soybean(Eunha, Pangsa and Paldal that have high yields) at various leaf temperature and light intensity at the stage of $V_5$. The relations between the Net photosynthesis rate and SLW(specific leaf weight) and chlorophyll content were also investigated. 1. Net photosynthesis rates at $25^{\circ}C$ were $21.5mgdm^{-2}h^{-1}$ in cv. Eunha, $20.2mgdm^{-2}h^{-1}$ in cv. Pangsa and $18.5mgdm^{-2}h^{-1}$ in cv. Paldal. 2. Most cultivars of Soybean showed the maximum rates of Net photosynthesis at $25^{\circ}C$, especially in cv. Eunha. Also Net photosynthesis rates differed depending on the leaf shape. Long leaf shape(cv. Eunha) was better than round leaf shape(cv. Paldal) in Net photosynthesis rate. 3. Respiration rates of leaves in Eunha, Pangse and Paldal were $0.56mgdm^{-2}h^{-1}$ at $15^{\circ}C$, $0.79mgdm^{-2}h^{-1}$ at $20^{\circ}C$ $1.15mgdm^{-2}h^{-1}$ at $25^{\circ}C$ and $1.37mgdm^{-2}h^{-1}$ at $30^{\circ}C$. 4. Specific leaf weight were $3.1mg/cm^2$ in Pangsa, $3.5mg/cm^2$ in Eunha and Paldal. No signlficant difference were showed in net photosynthesis rates and specific lear weight. 5. Leaf chlorophyll content were $2.48{\mu}g/gF.W.$ in Eunha, $2.19{\mu}g/gF.W.$ in Pangsa and $1.67{\mu}/g F.W.$ in Paldal. Significant difference were showed in Net photosynthesis rates and Leaf chlorophyll content. 6. The estimated compensation points at which net photosynthesis approached zero were $10{\mu}Em^{-2}s^{-1}$ in Eunha, Pangsa, and Palda at 1$5^{\circ}C$. The compensation point in cv. Eunha at $20^{\circ}C$ was $12P{\mu}Em^{-2}s{-1}$ while $13{\mu}Em^{-2}s{-1}$ in Pangsa and Palda. The compensation point in cv. Paldal at $25^{\circ}C$ was $18{\mu}Em^{-2}s{-1}$ while $16{\mu}Em^{-2}s{-1}$ in Eunha and Pangsa. The compensation point in cv. Palda at $30^{\circ}C$ was $23{\mu}Em^{-2}s{-1}$ Palda while $13{\mu}Em^{-2}s{-1}$ in Eunha and Pangsa.

• The Korean Journal of Nuclear Medicine
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• v.38 no.2
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• pp.180-189
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• 2004

Although the outcome of cancer patients after cytotoxic chemotherapy is related diverse mechanisms, multidrug resistance (MDR) for chemotherapeutic drugs due to cellular P-glycoprotein (Pgp) or multidrug-resistance associated protein (MRP) is most important factor in the chemotherapy failure to cancer. A large number of pharmacologic compounds, including verapamil, quinidine, tamoxifen, cyclosporin A and quinolone derivatives have been reported to overcome MDR. Single photon emission computed tomography (SPECT) and positron emission tomography (PET) are available for the detection of Pgp and MRP-mediated transporter. $^{99m}Tc$-MIBI and other $^{99m}Tc$-radiopharmaceuticals are substrates for Pgp and MRP, and have been used in clinical studies for tumor imaging, and to visualize blockade of PgP-mediated transport after modulation of Pgp pump. Colchicine, verapamil and daunorubicin labeled with $^{11}C$ have been evaluated for the quantification of Pgp-mediated transport with PET in vivo and reported to be feasible substrates with which to image Pgp function in tumors. Leukotrienes are specific substrates for MRP and $N-[^{11}C]acetyl-leukotriene$ E4 provides an opportunity to study MRP function non-invasively in vivo. SPECT and PET pharmaceuticals have successfully used to evaluate pharmacologic effects of MDR modulators. Imaging of MDR and reversal of MDR with bioluminescence in a living animal is also evaluated for future clinical trial. We have described recent advances in molecular imaging of MDR and reviewed recent publications regarding feasibility of SPECT and PET imaging to study the functionality of MDR transporters in vivo.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.3
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• pp.217-226
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• 2015

To investigate the distributions of BVOCs (Biogenic Volatile Organic Compounds) from mountain near mega city and their role in forest atmospheric, BVOCs and their oxidized species were measured at a 41 m tower in Mt. Taehwa during May, June and August 2013. A proton transfer reaction-mass spectrometer (PTR-MS) was used to quantify isoprene and monoterpenes. In conjunction with BVOCs, $O_3$, meteorological parameters, PAR (Photosynthetically Active Radiation) and LAI (Leaf Area Index) were measured. The average concentrations of isoprene and monoterpenes were 0.71 ppbv and 0.17 ppbv, respectively. BVOCs showed higher concentrations in the early summer (June) compared to the late summer (August). Isoprene started increasing at 2 PM and reached the maximum concentration around 5 PM. In contrast, monoterpenes concentrations began to increase 4 PM and stayed high at night. The $O_3$ maximum was generally found at 3 PM and remained high until 5 PM or later, which was concurrent with the enhancement of $O_3$. The concentrations of BVOCs were higher below canopy (18 m) than above canopy, which indicated these species were produced by trees. At night, monoterpenes concentrations were negatively correlated with these of $O_3$ below canopy. Using MEGAN (Model of Emissions of Gases and Aerosols from Nature), the emissions of isoprene and monoterpenes were estimated at 1.1 ton/year and 0.9 ton/year, respectively at Mt. Taehwa.