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Optimization of Submerged Culture Conditions for Protease Production and Its Enzymatic Properties (Protease 생산을 위한 최적 배양조건 및 생산된 Protease의 특성)

  • Cho, Hee-Yeon;Cho, Nam-Seok
    • Journal of the Korean Wood Science and Technology
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    • v.32 no.5
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    • pp.12-19
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    • 2004
  • This study was performed to investigate the optimum condition of protease production from submerged culture of oak mushroom (Lentinula edodes, Sanlim No. 5) and its enzymatic features. Among several combinations of media, the combination of wheat bran, corn flour, water and corn oil (WB+CF+W+ CO) yielded 84.8 U/g of maximum protease activity. This combination of ingredients, in spite of not being particularly protein-rich in comparison to the other media, allowed for good growth of the fungus and maximal protease production. Comparison of different growth medium liquids indicated that demineralized water afforded the best growth of the fungus and the highest protease activity. Acetate buffer and acidified water negatively affected The protease production peaked around 72 hr of incubation, and decreased thereafter. The molecular weights of produced protease were about 45,000 by Sephadex G-75 chromatography. The pH optimum for protease activity was 4, while maximal activity incubated at 37℃ for 1 hr was observed between pH 4~6. The optimum temperature of this protease was 55℃, and the enzyme was active over a broad temperature range (30~60℃), indicating that this protease would be suitable for a wide range of applications where. different pH and temperature are necessary, such as digestive aids, food industry, beer and tannery industries.

Investigation on the Farm Management and Livestock House Design Standard Perception to Enhance Usage of Livestock House Design Standard (축사표준설계도의 활용도를 높이기 위한 농가 운영 현황 및 축사표준설계도 인식 조사)

  • Kang, Sol-moe;Lee, In-bok;Hwang, Chang-kyu;Hwang, Soo-jin;Jeong, Deuk-young;Lee, Sang-yeon;Park, Se-jun;Choi, Young-bae;Kim, Da-in
    • Journal of The Korean Society of Agricultural Engineers
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    • v.64 no.6
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    • pp.87-99
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    • 2022
  • The meat consumption per person has continuously increased in recent years. However, the labor force in the domestic livestock industry has decreased due to the declining and ageing population. In order to increase productivity, the government have developed and distributed design standard of livestock houses. Presently, report showed that the adaptation rate of the developed livestock house design standard on the real farm was still low. Thus, this paper aimed to find ways to improve the utilization of the design standard through surveys. The survey was conducted on 650 farms across the country. Analysis of the result showed that in the poultry house, the unawareness of farmers to the design standard was found to be the biggest reason for not using the design standards. On the other hand, in the swine house, the previously built swine houses do not fit with the design standard. From these result, the following recommendations were suggested: 1) promotion and education are needed to enhance usage of design standard; 2) since it is impossible to make a design standard considering all the farm sites, it is important to consider the conditions of various farm site prior to enhancement of the design standard; 3) improvement factors such as reinforcing the ventilation design, reflecting animal welfare, preventing livestock diseases, and enhancing ICT devices can also be promoted.

A Study on Public Order Right Based on Analysis of the Administrative Disposition Results Against the Personal Information Protection Act Violation (개인정보 보호법 위반에 따른 행정처분 결과 분석을 통한 공표명령권 도입 연구 395)

  • Jeon, Ju Hyun;Rhee, Kyung Hyune
    • KIPS Transactions on Computer and Communication Systems
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    • v.11 no.11
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    • pp.395-402
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    • 2022
  • In case of violation of the Personal Information Protection Act, administrative dispositions will be taken according to the legal standards, and the results will be announced. However, the current method has limitations in its effectiveness as repeated administrative dispositions are increasing despite the announcement by the disclosure system of the Personal Information Protection Act. In this paper, we deploy the introduction of the 'public announcement commandment' against violators by analyzing the administrative disposition results according to the violation of the Personal Information Protection Act. It is able to strengthen the existing disclosure system for self-disclose violations by providing easy recognition to the people about the fact of violation itself against the Personal Information Protection Act. Furthermore, we analyze major industries through the industry groups and violations of laws that were subject to publication, and data published on the results of administrative dispositions for violation of the Personal Information Protection Act. Finally, we propose the legal basis for the 'public announcement commandment' which allows the violator to publish by oneself for the announcement of the fact that the corrective action has been taken.

Building plan research of Smart Ammunition Logistics System based on the 4th industrial technology (4차산업혁명기술 기반 스마트 탄약물류체계 구축 방안 연구)

  • Choi, Jong-Geun;Kim, Byung-Kyoo;Chang, Yoon Seok
    • Journal of Internet Computing and Services
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    • v.23 no.1
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    • pp.135-145
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    • 2022
  • This paper presented a method to build a predictable smart ammunition logistics system using the 4th industrial technology for ammunition logistics, which is the core functions in the field of defense and logistics. We have analyzed the current level of ammunition logistics with various perspectives such as domestic and overseas logistics policies, technology trends, ammunition logistics characteristics, the smart logistics certification measures by Ministry of Land, Infrastructure and Transport. As a result it is considered that the current ammunition logistics needs needs improvement. To improve this, we presented a direction based on the implications derived after analyzing various ongoing programs such as wired/wireless-based automation, smart ammunition depots, and logistics innovation of the army, navy, and air force that can be applied to the ammunition logistics. In order to implement a data-based smart ammunition logistics management system that can achieve innovation and efficiency of total life cycle while meeting changes in the battlefield environment, we presented 4 objectives such as "automation and modernization of field work", "3D-based storage management & improvement of issuing at war," and "data management for prediction-oriented ammunition management". it is expected that there will be benefits such as improvement of operational continuity, guarantee of ammunition reliability, budget reduction, improvement of inefficiencies such as delay, waiting, and double work, and reduction of accidents.

Experimental Comparison of Network Intrusion Detection Models Solving Imbalanced Data Problem (데이터의 불균형성을 제거한 네트워크 침입 탐지 모델 비교 분석)

  • Lee, Jong-Hwa;Bang, Jiwon;Kim, Jong-Wouk;Choi, Mi-Jung
    • KNOM Review
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    • v.23 no.2
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    • pp.18-28
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    • 2020
  • With the development of the virtual community, the benefits that IT technology provides to people in fields such as healthcare, industry, communication, and culture are increasing, and the quality of life is also improving. Accordingly, there are various malicious attacks targeting the developed network environment. Firewalls and intrusion detection systems exist to detect these attacks in advance, but there is a limit to detecting malicious attacks that are evolving day by day. In order to solve this problem, intrusion detection research using machine learning is being actively conducted, but false positives and false negatives are occurring due to imbalance of the learning dataset. In this paper, a Random Oversampling method is used to solve the unbalance problem of the UNSW-NB15 dataset used for network intrusion detection. And through experiments, we compared and analyzed the accuracy, precision, recall, F1-score, training and prediction time, and hardware resource consumption of the models. Based on this study using the Random Oversampling method, we develop a more efficient network intrusion detection model study using other methods and high-performance models that can solve the unbalanced data problem.

3D Digital Restoration of Koguryo Ceremonial Flag "Jeol" (고구려 의장기 절(節)의 3D 디지털 복원)

  • KONG, Jeonyoung;KONG, Seokkoo
    • Korean Journal of Heritage: History & Science
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    • v.55 no.3
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    • pp.6-20
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    • 2022
  • The restoration of cultural heritage materials is an important research theme. This study improved the existing cultural heritage restoration method and attempted to establish a restoration system for cultural heritage data based on historical documents and visual materials. Recognizing the limitations of existing studies, this paper attempted to restore cultural heritage data through interdisciplinary research. In addition, 3D restoration was carried out after restoration in 2D form based on literature documents rather than existing visual sources. The object of restoration that was selected was "Jeol," which represents the power of the king of Koguryo. Koguryo's Jeol is a type of flag. Jeol appears in the mural in Anak Tomb No. 3. Rather than using only photographic materials of murals, the restoration was carried out through cross-validation of literature data and materials on archaeological art history. This is important in that the restoration carried out in this study is an accurate restoration with a historical understanding based on the literature of the relevant cultural heritage. In this study, a restoration process based on historical records was established. A 3D restoration process was performed by adding and applying visual materials after the object was first shaped based on the literature data. Restoration based on literature and visual materials was carried out based on interdisciplinary research. Therefore, this study aims to build a digital restoration system for cultural heritages and to contribute to spreading the 3D digital restoration research of cultural heritages that can be applied to various platforms.

Viability of Vibrio parahaemolyticus and V. vulnificus isolated from human in cultured olive flounder (Paralichthys olivaceus) (Vibrio parahaemolyticus와 V. vulnificus 사람 분리균의 넙치, Paralichthys olivaceus에서의 생존율)

  • Im, Su Yeon;Kim, Eunheui
    • Journal of fish pathology
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    • v.35 no.1
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    • pp.57-63
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    • 2022
  • Vibrio parahaemolyticus and V. vulnificus are known to be infected to human via fisheries products. Therefore, food safety of fisheries products is important for public health and fish industry. This paper was conducted to know how well these human isolates can survive in olive flounder (Paralichthys olivaceus). The growth of V. parahaemolyticus and V. vulnificus showed about 50~60% reduced rates at 25℃ than at 37℃ and did not show any differences according to NaCl concentration of media except the increasing in the growth of V. vulnificus in medium containing 3% NaCl. Artificial infection of 1×106 CFU/fish was carried out to confirm the sensitivity of olive flounder against V. parahaemolyticus and V. vulnificus. After 1 week from injection, no fish was dead. To evaluate nonspecific defense of olive flounder against V. parahaemolyticus and V. vulnificus, the antibacterial potency of serum and epidermal mucus were tested. The number of the vibrios exposed to serum obtained from olive flounder significantly decreased after 3 hours, and epidermal mucus showed decrease of the bacteria over than 90% until 12 hours from exposure. Phagocytosis of head kidney leucocytes of healthy olive flounder against V. parahaemolyticus and V. vulnificus showed in over 70% of leucocytes at the 2 hours. Therefore, cultured olive flounder only as vehicle for human pathogen in environmental water is well developed its antibacterial potency against human pathogens, so the viability of V. parahaemolyticus and V. vulnificus in cultured olive flounder was considered very low.

Developement and Application of Patent Technology for the Improvement of Scaffolding Safety Facilities in Small-scale Muliti-Family Housing System for the Prevention of Severe Disasters (중대재해 예방을 위한 소규모 공동주택 시스템비계 안전시설물 개선 특허기술 개발 및 활용)

  • Kim, Sung Soo
    • Journal of Korean Society of Disaster and Security
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    • v.15 no.3
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    • pp.57-66
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    • 2022
  • According to the official letter of the Ministry of Employment and Labor (recent guidance on the status of deaths in the construction industry and thorough safety management, April 4, 2022), 17 deaths occurred in construction works worth more than 5 billion won until March 31, 2022. In terms of the size of the construction, 7 cases of 5 billion won to 12 billion won, 5 cases of 12 billion won to 80 billion won, and 5 cases of more than 80 billion won occurred. The causes of death accidents were found to be nine cases (50%), five cases (28%), two cases of collapse (11%), one case of entrapment (5%), and one case of drowning (5%). Safety facilities need to be strengthened to reduce deaths caused by falling, which accounts for most of the deaths, and if fall prevention nets, openings, and safety rails are completely installed, most falling accidents can be prevented. Therefore, this paper intends to present cases of development and use of patent technology through improvement of safety facilities in order to prevent accidents corresponding to the fall of new urban living housing sites, which are small apartment houses.

Card Transaction Data-based Deep Tourism Recommendation Study (카드 데이터 기반 심층 관광 추천 연구)

  • Hong, Minsung;Kim, Taekyung;Chung, Namho
    • Knowledge Management Research
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    • v.23 no.2
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    • pp.277-299
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    • 2022
  • The massive card transaction data generated in the tourism industry has become an important resource that implies tourist consumption behaviors and patterns. Based on the transaction data, developing a smart service system becomes one of major goals in both tourism businesses and knowledge management system developer communities. However, the lack of rating scores, which is the basis of traditional recommendation techniques, makes it hard for system designers to evaluate a learning process. In addition, other auxiliary factors such as temporal, spatial, and demographic information are needed to increase the performance of a recommendation system; but, gathering those are not easy in the card transaction context. In this paper, we introduce CTDDTR, a novel approach using card transaction data to recommend tourism services. It consists of two main components: i) Temporal preference Embedding (TE) represents tourist groups and services into vectors through Doc2Vec. And ii) Deep tourism Recommendation (DR) integrates the vectors and the auxiliary factors from a tourism RDF (resource description framework) through MLP (multi-layer perceptron) to provide services to tourist groups. In addition, we adopt RFM analysis from the field of knowledge management to generate explicit feedback (i.e., rating scores) used in the DR part. To evaluate CTDDTR, the card transactions data that happened over eight years on Jeju island is used. Experimental results demonstrate that the proposed method is more positive in effectiveness and efficacies.

Research Trend on ESG Management of Corporation (기업의 ESG 경영에 대한 국내·외 연구동향)

  • Byun, Youngjo;Woo, Seung Han
    • Clean Technology
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    • v.28 no.2
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    • pp.193-200
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    • 2022
  • The term environmental, social and governance (ESG) was first used in the 2003 United Nations Environmental Programme Finance Initiative (UNEP FI). Among the three areas of ESG, environment refers to the impact of companies on the environment. Environmental factors address climate change policies and attempts to reduce emissions, waste and natural resource consumption. Social factors refer to the direction in which a company can improve the social impact of stakeholder includes employees, customers, communities, and governments involved in direct or indirect interaction with the organization itself and the company. Governance factors refer to stakeholders who make major decisions, the composition of the board of directors, their diversity and independence, and the internal policies that set limits and expectations for decision-making. Research related to ESG management is part of corporate social responsibility, sustainability, corporate or financial performance, and social responsibility investment. Through case studies and data-based empirical studies, it was confirmed that ESG management companies had positive results for most of the ESG related fields. Through literature analysis of domestic and international ESG history, introduction background, and management performance, this paper presents theoretical, practical implications by confirming that ESG's introduction and operation strategies are strong competitive strategies that directly affect corporate growth by creating attractive factors.