• 제목/요약/키워드: Panax species

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Panax(인삼)속 식물의 사포닌화합물 함량 및 조성 (Content and Composition of Saponin Compounds of Panax Species)

  • 고성룡;최강주
    • Journal of Ginseng Research
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    • 제19권3호
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    • pp.254-259
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    • 1995
  • The content and composition of saponin compounds of Panax species were analyzed according to their species, region and processing type of red and white ginseng. The species employed were Korean-, Chinese-, Japanese red ginsengs, and Korean white ginseng of Panax ginseng, American- and Canadian ginsengs of Panax quinquefolium, and Panax notoinseng. Twelve main saponin components in the ginseng were identified and quantified using TLC and HPLC. All three species had remarkably different content and composition. However, within each species they were similar. Twelve major ginsenosides were determined in P. ginseng, eight in p. quinquefolium, and six in P. notoginseng. Of the components of P ginseng Rf, $Rh_1$, $Rh_2$ and Ra were not detected in P quinquefolium, and $Rb_2$, Rc, Rf, $Rh_2$, Ra and Ro not detected in P. notoinseam. Crude saponin content and protopanaxadiol/protopanaxatriol saponin ratio were compared. They were 4.81~5.24% and 1.27~ 1.45 in p. ginsengs, 7.01~7.25% and 2.12~ 2.15 in p. quinquefolium, 9.80% and 0.99 in P. notoineng. The prosapogenin and sapogenin content were different among the Panax species.

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Potable handheld gas chromatograph(PHGC)를 이용한 인삼속(Panax species) 식물들의 향기패턴 분석 (Analysis of Aroma Pattern of Panax Species by Potable Handheld Gas Chromatograph)

  • 이부용;양영민;이옥환;김경임
    • 한국식품과학회지
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    • 제34권5호
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    • pp.862-866
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    • 2002
  • 분말상태의 인삼속 식물들의 품종 및 원산지를 판별하기 위하여 SAW 센서가 내장된 PHGC를 이용하여 향기 패턴을 분석하였다. 한국 백삼을 1로 기준할 때 전체적인 Rt에 대한 frequency pattern의 면적비는 화기삼 $0.248{\sim}0.871$, 전칠삼 $0.030{\sim}0.674$, 중국산 백삼 $0.005{\sim}0.212$ 범위로 나타났다. 분명한 차이를 나타내는 몇 개의 특정 향기성분의 면적비를 보면 $Rt_{20.02}$에서 한국산 백삼 1일 때, 중국산 백삼 0.212, 화기삼 0.343, 전칠삼 0.065이었다. 또한 $Rt_{21.70}$$Rt_{24.90}$에서 검출되는 향기성분의 면적비도 품종간의 차이가 뚜렷하였다. 한국산 백삼과 중국산 백삼에서 검출된 Rt_26.15 향기성분의 면적비는 각각 1과 0.185로 나타나 원산지간의 차이도 분명히 나타났다. $Rt_{26.15}$의 향기성분은 화기삼과 전칠삼에서는 검출되지 않았다. Frequency pattern, derivative pattern을 Vapor $print^{TM}$을 사용하여 도형화하여 비교한 결과 한국 백삼(Korean Panax ginseng C.A. Meyer), 화기삼(미국삼, 서양삼, Panax quinquefolium L.), 전칠삼(Panax notoginseng F.H. Chen), 중국산 백삼(Chinese Panax ginseng)은 서로 다른 패턴을 보여주어 품종간의 차이는 물론 원산지의 차이도 뚜렷하게 나타났다.

DNA 바코딩과 고해상 융해곡선분석에 기반한 인삼속 식물의 종 판별 (Internal Transcribed Spacer Barcoding DNA Region Coupled with High Resolution Melting Analysis for Authentication of Panax Species)

  • 방경환;김영창;임지영;김장욱;이정우;김동휘;김기홍;조익현
    • 한국약용작물학회지
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    • 제23권6호
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    • pp.439-445
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    • 2015
  • Background : Correct identification of Panax species is important to ensure food quality, safety, authenticity and health for consumers. This paper describes a high resolution melting (HRM) analysis based method using internal transcribed spacer (ITS) and 5.8S ribosomal DNA barcoding regions as target (Bar-HRM) to obtain barcoding information for the major Panax species and to identify the origin of ginseng plant. Methods and Results : A PCR-based approach, Bar-HRM was developed to discriminate among Panax species. In this study, the ITS1, ITS2, and 5.8S rDNA genes were targeted for testing, since these have been identified as suitable genes for use in the identification of Panax species. The HRM analysis generated cluster patterns that were specific and sensitive enough to detect small sequence differences among the tested Panax species. Conclusion : The results of this study show that the HRM curve analysis of the ITS regions and 5.8S rDNA sequences is a simple, quick, and reproducible method. It can simultaneously identify three Panax species and screen for variants. Thus, ITS1HRM and 5.8SHRM primer sets can be used to distinguish among Panax species.

Comprehensive comparative analysis of chloroplast genomes from seven Panax species and development of an authentication system based on species-unique single nucleotide polymorphism markers

  • Nguyen, Van Binh;Giang, Vo Ngoc Linh;Waminal, Nomar Espinosa;Park, Hyun-Seung;Kim, Nam-Hoon;Jang, Woojong;Lee, Junki;Yang, Tae-Jin
    • Journal of Ginseng Research
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    • 제44권1호
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    • pp.135-144
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    • 2020
  • Background: Panax species are important herbal medicinal plants in the Araliaceae family. Recently, we reported the complete chloroplast genomes and 45S nuclear ribosomal DNA sequences from seven Panax species, two (P. quinquefolius and P. trifolius) from North America and five (P. ginseng, P. notoginseng, P. japonicus, P. vietnamensis, and P. stipuleanatus) from Asia. Methods: We conducted phylogenetic analysis of these chloroplast sequences with 12 other Araliaceae species and comprehensive comparative analysis among the seven Panax whole chloroplast genomes. Results: We identified 1,128 single nucleotide polymorphisms (SNP) in coding gene sequences, distributed among 72 of the 79 protein-coding genes in the chloroplast genomes of the seven Panax species. The other seven genes (including psaJ, psbN, rpl23, psbF, psbL, rps18, and rps7) were identical among the Panax species. We also discovered that 12 large chloroplast genome fragments were transferred into the mitochondrial genome based on sharing of more than 90% sequence similarity. The total size of transferred fragments was 60,331 bp, corresponding to approximately 38.6% of chloroplast genome. We developed 18 SNP markers from the chloroplast genic coding sequence regions that were not similar to regions in the mitochondrial genome. These markers included two or three species-specific markers for each species and can be used to authenticate all the seven Panax species from the others. Conclusion: The comparative analysis of chloroplast genomes from seven Panax species elucidated their genetic diversity and evolutionary relationships, and 18 species-specific markers were able to discriminate among these species, thereby furthering efforts to protect the ginseng industry from economically motivated adulteration.

인삼 산성다당체의 삼류간 및 부위별 함량비교 (Comparisons of Acidic Polysaccharide Content in Various Ginseng Species and Parts)

  • 도재호;이형옥
    • Journal of Ginseng Research
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    • 제17권2호
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    • pp.145-147
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    • 1993
  • The amounts of ginseng acidic polysaccharide (GAP) in red ginseng (Panax ginseng) were higher than those of wild and cultured Panax quinquefolius, Panax notoginseng as well as white ginseng (Panax ginseng). In white ginseng, there is no difference in the GAP amount among root ages or sizes. Also, the GAP amount of red ginseng body was similar to that of ginseng rhizome, but was higher than that of leaf and epidermis.

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Molecular Differentiation of Panax Species by RAPD Analysis

  • Shim, Young-Hun;Choi, Jung-Ho;Park, Chan-Dong;Lim, Chul-Joo;Cho, Jung-Hee;Kim, Hong-Jin
    • Archives of Pharmacal Research
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    • 제26권8호
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    • pp.601-605
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    • 2003
  • Traditional taxonomic methods used for the identification and differentiation of ginsengs rely primarily on morphological observations or physiochemical methods, which cannot be used efficiently when only powdered forms or shredded material is available. Randomly amplified polymorphic DNA (RAPD) was used to determine the unique DNA profiles that are characteristic not only of the genus Panax but also of various Panax subgroups collected from five different countries. RAPD results of OP-5A primer showed a specific single band that is characteristic of all ginseng samples. RAPD results of OP-13B primer demonstrated that OP-13B primer could be used as a unique RAPD marker to differentiate Panax species. These results support that this approach could be applied to distinguish Korean Ginseng (Panax ginseng) from others at the molecular level.

Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products

  • Jung, Juyeon;Kim, Kyung Hee;Yang, Kiwoung;Bang, Kyong-Hwan;Yang, Tae-Jin
    • Journal of Ginseng Research
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    • 제38권2호
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    • pp.123-129
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    • 2014
  • Korean ginseng (Panax ginseng) and American ginseng (Panax quinquefolius) are widely used medicinal plants with similar morphology but different medicinal efficacy. Roots, flowers, and processed products of Korean and American ginseng can be difficult to differentiate from each other, leading to illegal trade in which one species is sold as the other. This study was carried out to develop convenient and reliable chloroplast genome-derived DNA markers for authentication of Korean and American ginseng in commercial processed products. One codominant marker could reproducibly identify both species and intentional mixtures of the two species. We further developed a set of species-unique dominant DNA markers. Each species-specific dominant marker could detect 1% cross contamination with other species by low resolution agarose gel electrophoresis or quantitative polymerase chain reaction. Both markers were successfully applied to evaluate the original species from various processed ginseng products purchased from markets in Korea and China. We believe that high-throughput application of this marker system will eradicate illegal trade and promote confident marketing for both species to increase the value of Korean as well as American ginseng in Korea and worldwide.

Cytohistological study of the leaf structures of Panax ginseng Meyer and Panax quinquefolius L.

  • Lee, Ok Ran;Nguyen, Ngoc Quy;Lee, Kwang Ho;Kim, Young Chang;Seo, Jiho
    • Journal of Ginseng Research
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    • 제41권4호
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    • pp.463-468
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    • 2017
  • Background: Both Panax ginseng Meyer and Panax quinquefolius are obligate shade-loving plants whose natural habitats are broadleaved forests of Eastern Asia and North America. Panax species are easily damaged by photoinhibition when they are exposed to high temperatures or insufficient shade. In this study, a cytohistological study of the leaf structures of two of the most well-known Panax species was performed to better understand the physiological processes that limit photosynthesis. Methods: Leaves of ginseng plants grown in soil and hydroponic culture were sectioned for analysis. Leaf structures of both Panax species were observed using a light microscope, scanning electron microscope, and transmission electron microscope. Results: The mesostructure of both P. ginseng and P. quinquefolius frequently had one layer of non-cylindrical palisade cells and three or four layers of spongy parenchymal cells. P. quinquefolius contained a similar number of stomata in the abaxial leaf surface but more tightly appressed enlarged grana stacks than P. ginseng contained. The adaxial surface of the epidermis in P. quinquefolius showed cuticle ridges with a pattern similar to that of P. ginseng. Conclusion: The anatomical leaf structure of both P. ginseng and P. quinquefolius shows that they are typical shade-loving sciophytes. Slight differences in chloroplast structure suggests that the two different species can be authenticated using transmission electron microscopy images, and light-resistant cultivar breeding can be performed via controlling photosynthesis efficiency.

인삼(Panax)속 식물의 일반성분, 무기성분, 아미노산 및 유리당 함량조성 (Comparison of Proximate Composition, Mineral Nutrient, Amino Acid and Free Sugar Contents of Several Panax Species)

  • 고성룡;최강주
    • Journal of Ginseng Research
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    • 제20권1호
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    • pp.36-41
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    • 1996
  • Chemical components of Panax (P) species were compared. p. species used were Korean white ginseng, Korean, Chinese and Japanese red ginseng (P ginseng), American and Canadian ginseng (P. quinquefolium) , and sanchl ginseng (P. notoginseng). No significant difference in the proximate contents was observed among P. species. Ash, crude lipld and total sugar contents in root of P. notoginseng were found to be relatively lower than those of P. ginseng and P. quinquefolium, but the contents of crude protein and crude fiber were similar among those ginsengs. Mineral nutrient con tents showed a little difference among ginseng species. Total nitrogen contents were slightly higher in P. ginseng than P. quinquefolium and P. notoginseng and Fe and Cu were lower in Chinese and Japanese red ginsengs. Kinds and compositions of amino acids were similar but contents of amino acids were different among ginseng species. Total amino acid contents were 76.3∼83.9 mg/g in P. ginseng 53.8∼60.4 mg/g in p. quinquefolium and 54.9 mg/g in P notoginseng. Free sugar contents were lower in P. notoginseng than P. ginseng or P. quinquefolium. Sucrose accounted for 90∼92% of total free sugar contents with relatively high content in white ginsengs, while sucrose and maltose were 32-36% and 55∼60%, respectively, in red ginseng.

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미토콘드리아 DNA 염기서열 변이를 이용한 인삼 종 판별 연구 (Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species)

  • 조익현;방경환;김영창;김장욱;신미란;문지영;노봉수;현동윤;김동휘;차선우;김홍식
    • 한국약용작물학회지
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    • 제21권2호
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    • pp.91-96
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    • 2013
  • This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as 'AA', 'BA' and 'AB', respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.