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http://dx.doi.org/10.7783/KJMCS.2013.21.2.91

Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species  

Jo, Ick Hyun (Department of Herbal Crop Research, NIHHS, RDA)
Bang, Kyong Hwan (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Young Chang (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Jang Uk (Department of Herbal Crop Research, NIHHS, RDA)
Shin, Mi Ran (Department of Herbal Crop Research, NIHHS, RDA)
Moon, Ji Young (Experiment Research Institute of National Agricultural Products Quality Management Service, MIFAFF)
Noh, Bong Soo (Department of Food Science and Technology, Seoul Women's University)
Hyun, Dong Yun (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Dong Hwi (Department of Herbal Crop Research, NIHHS, RDA)
Cha, Seon Woo (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Hong Sig (Department of Plant Resources, Chungbuk National University)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.2, 2013 , pp. 91-96 More about this Journal
Abstract
This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as 'AA', 'BA' and 'AB', respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.
Keywords
Panax Species; coxl; nad1/2-3; nad2/1-2; Mitochondria; Species-Specific Polymorphisms;
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Times Cited By KSCI : 6  (Citation Analysis)
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