• Toxicological Research
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• 제11권2호
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• pp.235-239
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• 1995

This study was conducted to investigate the metallothionein (MT) induction by Panax ginseng C. A. Meyer in cadmium chloride intoxication. The results were as follows: Generally, detoxicatlon effects by Panax ginseng C. A. Meyer extract increased proportionally to the increase of cadmium concentrations. When a 8 mg/g dosage of cadmium was administered, formation of the cadmium and EDTA complex showed the highest antitoxic effect. Also, when a 4 mg/g dosage of cadmium was administered, Panax ginseng C. A. Meyer extract showed the highest antitoxic effect in metallothionein induction. According to the above results, Panax ginseng C. A. Meyer extract administered with cadmium increased metallothionein concentration and decreased the toxicity of cadmium in liver.

• Journal of Ginseng Research
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• 제18권1호
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• pp.44-48
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• 1994

In adrenaline-stimulated human platelets, panaxadiol (PD) and panaxatriol (PT) from Panax ginseng C.A. Meyer did not inhibit the $Ca^{2+}$-innux, but inhibited the formation of thromboxane $A_2$ and the platelet aggregations. It seems that PD and PT block a pathyway interconvefing arachidonic acids (20:4) to thromboxane $A_2$ (TX $A_2$), because the amount of $Ca^{2+}$ which phospholipase C or phospholipase $A_2$ requires to liberate 20 : 4 from membrane phospholipids was increased by PD and PT. These results mean that PH and PT have an antiplatelet effect by Inhibiting the formation of TX $A_2$.

• 한국식품영양학회지
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• 제5권2호
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• pp.144-149
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• 1992

Invertase was extracted from Korean ginseng(Panax ginseng C. A. Meyer) leaf with deionized water, and then prepared by ammonium sulfate(0.4~0.6 Sat.) fractionation, the enzymological properties of the invertase were investigated, and the results obtained were as follows. The optimum pH and temperature of the enzyme were pH 6.0 and 4$0^{\circ}C$ respectively. The enzyme was stable in the pH range of pH 6.0 to 8.0, and at the temperature below 4$0^{\circ}C$. The enzyme was inactivated completely by the treatment with some proteases(pepsin, trypsin, papain and ficin) and protein denaturants(8M urea and 6M guanidine-HCI), but not with glycosidases (a-amylase, $\beta$-amylase and glucoamylase). The enzyme catalyzed specifically the hydrolization of the $\beta$-fructofuranosides such as sucrose and inulin.

• Journal of Ginseng Research
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• 제17권3호
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• pp.246-249
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• 1993

Hexane, Hexane/diethylether and chloroform fractions from Panax ginseng C.A. Meyer stroungly inhibitied human platelet aggregation induced by a high dose of thrombin (2$\mu$/ml). Chloroform fraction more strongly inhibited the platelet aggregation than the other two fraction among them. There were fatty acid ester and phosphate ester instead of polyacethylene compounds in the chloroform fraction.

• Journal of Ginseng Research
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• 제10권2호
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• pp.159-166
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• 1986

Effects of plant growth regulators on the germination of ginseng (Panax ginseng C.A. Meyer) seeds were investigated. Ginseng seeds germinated more vigorously in the treatments of kinetin and BA, and the promoting effect of kinetic on the germination and the growth of rootlet enhanced in low temperature ($10^{\circ}C$). However, GA did not promote the germination of dehiscent seed. The optimum temperature for germination of dehiscent seed was $10^{\circ}C$ and the range of effective concentration of kinetin for germination was 50 to 100 ppm.

• Archives of Pharmacal Research
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• 제5권2호
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• pp.45-52
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• 1982

Staibilization effect of Panax ginseng C. A. Meyer on .betha.-D-Galactosidase inactivation was proved by kinetic studies of thermal inactivation of the enzyme. The water extract Panax ginseng C. A. Meyer showed stabilization activity at minimal concentration of 10ppm. The methanolic extract was purified to obtain ginseng saponins, and two groups of the ginsenosides, i. e. protopanaxadiol and protopanaxatriol were isolated. They also showed a protective effect against the thermal and chemical inactivation of the enzyme; p-chloromercuribenzoic acid and hydroxylamine known as protein modifier greatly inactivated the enzyme but inactivation was significantly balocked by the ginseng component MG$^{2+}$, known as a cofactor, stabilized the enzyme and the poor stabilization effect by it was potentiated by ginseng components.s.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.376.2-377
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• 2002

Some of the dammarane-type saponins. ginsenosides of Panax ginseng C.A. Meyer (Araliaceae) are now well established as a potent chemotherapeutic agent against a wide variety of aliments. Its various pharmacological and biological activities have been thoroughly reviewed (S. Shibata, 2001). The limited supply of the drug from the original source. the hairy root of the Panax ginseng promoted intense efforts to develop alternate sources and means of production. (omitted)

• Journal of Plant Biology
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• 제35권2호
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• pp.99-106
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• 1992

인삼(Panax ginseng C.A. Meyer) 종자단백질인 vicilin을 ammonium sulfate 침전, gel permeation 및 이온 교환 크로마토그래피로 정제하였다. Vicilin은 분자량 55,000(큰 소단위) 및 44,000(작은 소단위)인 두 종의 소단위를 포함하는 당단백질이다. Vicilin에 대한 항체를 토끼에서 형성시켜 DEAE-Affi-Gel Blue affinity 크로마토그래피로 정제하였다. 이 항체와 금 입자가 결합된 2차 항체를 종자의 배유세포에 반응시켰다. 금 입자는 배유세포내의 단백질체, 전자밀도가 높은 과립 및 골지체의 elaborating 과립에 표지되었다. 이러한 결과는 조면소포체에서 합성되어 골지체로 수송된 vicilin이 골지의 소포내에서 공정과정을 거쳐 전자밀도가 높은 과립이 된 다음 단백질로 수송됨을 나타낸다.

• Journal of Plant Biology
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• 제32권3호
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• pp.145-150
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• 1989

Mature zygotic embryos dissected from ginseng(Panax ginseng C. A. Meyer) seeds were cultured on Murashige and Skoog's (MS) medium containing various concentrations of 2, 4-dichlorophenoxyacetic acid(2, 4-D) and kinetin. Somatic embryos were induced directly from cotyledonary tissue or from intervening callus. The induction frequency of somatic embryos was up to 55%. Upon transfer to half-strength MS medium supplemented with 1 mg/1 6-benzyladenine(BA) and 1 mg/1 GA3, most somatic embryos developed into plantlets. Over 50% of the plantlets flowered after 4 weeks of culture and then a few bore immature fruits in vitro. Therefore, it is suggested that the juvenility of the ginseng tissue which give rise to somatic embryos does not interfere with in vitro flowering of their regenerated plantlets.

• Journal of Ginseng Research
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• 제14권1호
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• pp.14-20
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• 1990

In An invertase (EC 3.2.1.26) was extracted from Korean giseng (Panax ginseng C.A. Meyer) with distilled tvater The ginseng invertase was purified about 62.6 folds purified by procedures including ammonium sulfate fractionation , DEAE-cellulofine chromatography and gelfiltrations through Sephadex G-75 and the recovery of enzyme activity was 11.1%. The homogeneity of the purified enzyme was probed by polyacrylamide gel disc electrophoresis. The purifled enzyme was divided into two different subunits by treating with a mixture of SDS and 2-mercautoethanol, and the molecular weight of the large subunit was estimatedtobe 116,000 and that of the small one to be 14,000. The optimal VH and temperature of the enzyme were pH 6 and 45$^{\circ}C$, respectively. The enzyme hydrolyzed specifically the hydrolyzation of the -fructofuranosides such as sucrose, raffinose and inulin. The Km values of the enzyme for sucrose and raffinose were determined to be 0.85 and 0.6 mM, respectively.