• Herbal Formula Science
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• v.32 no.1
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• pp.83-90
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• 2024

Objectives : As a substitute for high-price ginseng, this study attempted to examine a possibility of the ferment extract of Panax ginseng sprout whether leaves and roots can be used together as a cosmetic ingredient with anti-oxidative and wrinkle-care effects. Methods : In terms of a test method, antioxidant activities were confirmed through total polyphenol contents, total flavonoid contents, DPPH radical scavenging activity and ABTS radical scavenging activity using the Panax ginseng sprout. In addition, to assess wrinkle-care effectiveness, the cytotoxicity of the extract was analyzed through MTT assay, and inhibition of collagenase activity in the cells was tested using the Panax ginseng sprout fermented by Saccharomyces cerevisiae. Resuits : The content of polyphenols and flavonoids in natural plants was highest in Panax Ginseng Sprout Extract at 100℃, which also demonstrated high DPPH, ABTS radical scavenging activity. MTT assay demonstrated that the Panax Ginseng Sprout Ferment Extract did not have a cytotoxic effect in CCD-986SK cell. Also, Panax Ginseng Sprout Ferment Extract was found to inhibit MMP-1 expression by 51.85±6.09% at a concentration of 10%. Conclusions : Therefore, this study has confirmed a possibility of Panax ginseng sprout ferment extract as a cosmetic ingredient with MMP-1-inhibitory effects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.5
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• pp.711-716
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• 2016

The purpose of this study was to evaluate the quality of castella added with different concentrations (0%, 5%, 10%, and 15%) of Panax ginseng sprout powder. The specific gravity of castella made with 15% ginseng sprout powder was the highest (0.65), and baking loss rate was highest well. However, the height and weight of castella were highest in the control. The pH of castella decreased with increasing levels of Panax ginseng sprout powder, whereas sugar content of castella did not show significant differences. The Hunter L, a, and b values of crust decreased as the concentration of Panax ginseng sprout powder increased. The Hunter L and a values of crumb decreased as the concentration of ginseng sprout powder increased, whereas b values of crumb increased. The gumminess and chewiness of castella increased by addition of Panax ginseng sprout powder, whereas cohesiveness of castella decreased. The hardness and springiness of castella did not show significant differences. In the sensory evaluation, crust color, crumb color, aroma and moistness did not show significant differences among samples while sweet taste, and chewiness were highest in the control group. The overall acceptability of castella added with 5% Panax ginseng sprout powder was the highest. Therefore, the results suggest that castella added with 5% ginseng sprout powder could be helpful for improving physical quality and taste.

• Journal of Dairy Science and Biotechnology
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• v.39 no.4
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• pp.157-165
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• 2021

This study aimed to determine the effect of extraction temperature and time on the antioxidant activity of hot water extract from Panax ginseng sprout powder and to evaluate the suitability of this extract for use in dairy products. Water-soluble fractions of commercial Panax ginseng sprout powder were obtained by hot water extraction at 25, 60, or 80℃ for 0.5, 2, 12, or 24 h. The antioxidant activity of each extract was evaluated by measuring its free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). DPPH and ABTS free radical scavenging activity increased with extraction temperature from 25 to 80℃. At 80℃, increasing the extraction time from 0.5 to 2 h led to increases in DPPH and ABTS free radical scavenging activity. Thus, the extract obtained under 2 h at 80℃ was selected for addition to milk and yogurt. After 16 days of storage, there were no significant changes in the pH of the milk or the antioxidant activity of the extract. With regard to yogurt fermentation, adding the extract did not affect the pH or the number of viable lactic acid bacteria. In conclusion, hot water extract from Panax ginseng sprout powder can be added to dairy products to enhance antioxidant activity.

• Food Science and Preservation
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• v.9 no.2
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• pp.168-173
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• 2002

Growth characteristics and crude protein content of soybean sprouts cultivated with extracts of some Korean herb medicines for 4 days were investigated. Extracts of Korean herb medicines were utilized and adjusted to 100 ppm, 200 ppm, 300 ppm and 400 ppm as culturing water of soybean sprouts. Of Korean herb medicines tested, extract of Panax ginseng was most effective to increase of weight and length of soybean sprouts, followed by Glycyrrhiza glabra. Extracts of Angelica gigas and Cnidium officinale were similar with that of control group. Extract of Coptidis japonica inhibited the growth of soybean sprout. Optimal concentrations of extracts of Glycyrrhiza glabra and Panax ginseng for growth of soybean sprout were 100 ppm and 200 ppm, respectively. Weight of soybean sprout cultivated with extract of Panax ginseng (PGE) was increased to 7.2%(wet basis) as compared with that of control group. Length of soybean sprouts cultivated with 200 ppm of PGE was accelerated to 32% and increased to 50% in soybean sprout cultivated for 3 days. Crude protein contents of soybean sprout cultivated with extract of Angelica gigas or Panax ginseng was slightly increased according to cultivation period. In Cnidium officinale group, crude protein contents was increased at a 300∼400 ppm concentration and 4 days of cultivation.

• Journal of Life Science
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• v.13 no.3
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• pp.273-279
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• 2003

Changes in weight, length, amino acids, organic acids and free sugars of soybean sprouts germinated with extract of Panax ginseng(PGE, 100∼400 ppm) were investigated. PGE increased the weight and length of soybean sprouts. Content of total amino acid in soybean sprout germinated at 100 ppm of PGE after cultivation for 3 days was the most abundant and then decreased by increasing the concentration of PGE. Content of aspartic acid was increased with culture time, but that of glutamic acid was shown to be an opposite trend. Content of total free sugar was increased by increasing culture time and not affected by concentration of PGE. Content of sucrose in control group during growth of soybean sprout was decreased, but sucrose contents in PGE groups were increased to 3 days and decreased thereafter. However, the other sugars were continuously increased for 4 days. Content of total organic acids was the most abundant in soybean sprouts germinated with 200 ppm of PGE and cultured for 3 days. Phytic acid was a major organic acid, showing the range of 45 to 60% for total organic acids. In conclusion, PGE as sprouting water of soybean was effective to increase of growth, contents of amino acids and organic acids in soybean sprouts, indicating that PGE accelerated the quality of soybean sprouts.

• Korean Journal Plant Pathology
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• v.11 no.1
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• pp.39-46
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• 1995

Ditylenchus destructor isolated from diseased ginseng roots was cultured on ginseng calli, fungal mycelium (Fusarium solani), carrot discs and radish sprouts. Effects of temperature, organic material and flooding on the nematode population changes were examined. D. destructor multiplied readily on the culture media except radish sprout medium, and was cultured best on the fungal culture at 2$0^{\circ}C$. Feeding of the fungal hypha and radish root hairs, molting and mating in the fungal culture medium were observed. Addition of organic materials (perilla, sesame, soybean and ginseng leaves) in soil significantly increased Aphelenchus avenae and saprophytic nematode populations, while D. destructor populations changed little and the nematode population growths were limited by the organic amendments (except sesame leaves). The nematode populations in soil including D. destructor were decreased by flooding. The results indicate that D. destructor may survive but not multiply readily in soil without host plants and that it can be effectively controlled by flooding.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.79-79
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 저장온도가 묘삼의 새싹인삼으로의 생장과 품질에 미치는 영향을 조사하여 묘삼의 저장에 적합한 온도를 구명하고자 수행하였다. 2년생 묘삼을 50 ㎛ LDPE 필름에 100g씩 넣어 포장하였고 온도 0, -2, -4℃에서 10개월 동안 저장하면서 2개월 마다 꺼내어 5℃ 저장고로 이동하여 5~7일 온도순화처리를 거친 후 실험을 실시하였다. 1차 육안조사로 묘삼의 부패율과 2차 생육조사로 묘삼을 재식 후 새싹인삼으로 재배 후 생장 상태와 품질을 조사한 결과 저장 4개월 이후 묘삼의 생육조사 시 새싹인삼의 생장과 품질이 묘삼의 저장온도에 따라 유의적인 차이를 보였다. 즉, 저장 4개월 된 묘삼의 건전한 새싹인삼으로의 생장은 0℃ 저장 묘삼에서 58.3%, -2℃ 저장 묘삼에서 72.1%, -4℃ 저장 묘삼에서 37.2%로 조사되었고, 저장 8개월 된 묘삼은 0℃ 저장 묘삼에서 9.7%, -2℃ 저장 묘삼에서 54.3%, -4℃ 저장 묘삼에서 6.9%로 조사되었다. 특히 0℃ 저장 묘삼은 저장 2개월에 출아가 진행되었고 저장기간이 지날수록 출아된 싹이 동해의 피해를 받는 것으로 조사되었다. 묘삼의 생육특성인 엽장, 엽폭, 경장, 근장을 측정한 결과 -2℃에 저장된 묘삼의 엽폭과 경장의 길이가 유의적으로 긴 것으로 조사되어 새싹인삼 재배를 위한 묘삼의 적정 저장온도는 -2℃로 설정하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.81-81
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 사용되는 필름의 종류가 묘삼의 출아율이나 생육에 영향을 미치는 지 조사하기 위해 수행되었다. 포장에 사용된 필름은 다공성물질인 제올라이트와 페그마타이트가 5% 함유된 50㎛ low density polyethylene(기능성 LDPE) 필름과 nylon/polyethylene 80 ㎛(Ny/PE) 필름에 묘삼을 100g씩 포장한 후 골판지 상자에 담아 -2℃의 저장고에서 10개월 동안 저장하면서 8개월째부터 2개월마다 시료를 꺼내어 5℃에서 5일 동안 온도 순화를 시킨 후 1차 조사에서는 육안조사로 실험실에서 건전한 묘삼과 부패 묘삼을 조사하였고, 건전한 묘삼만 새싹인삼 수경재배 농가에 재식한 후 2차 조사로 묘삼의 출아율과 출아된 묘삼이 새싹인삼으로 건전하게 생육한 비율을 조사하였다. 조사결과 Ny/PE 필름에 8개월 저장된 묘삼의 경우 1차 육안검사 시 뇌두부위가 검게 변하여 묘삼이 대부분 고사된 것으로 조사되었다. 필름 개봉 전 필름 내부의 O₂ 및 CO₂ 가스농도를 측정한 결과 O₂ 농도는 1.91%, CO₂ 농도는 38.9%로 측정되었고, 필름 개봉 시 알코올 냄새의 이취가 나는 것으로 보아 Ny/PE 필름으로 포장 된 묘삼이 -2℃의 저온저장기간 동안에도 호흡을 하면서 필름 내 산소를 완전히 소모하고 혐기적인 환경으로 변화시켜 대사활동이 불가능하였기 때문에 뇌두의 싹이 고사된 것으로 조사되었다. 반면 기능성 필름으로 포장한 묘삼은 저장 8개월에 정식 후 74.3%의 출아율을 보였고, 건전한 새싹인삼의 비율은 67.2%로 조사되었다. 정식 전·후 묘삼의 개체수 대비 건전 출아율의 비율은 56.7%를 나타내어 Ny/PE 필름으로 포장한 묘삼의 결과와 큰 차이를 보였다. 기능성필름의 개봉 전 필름 내부의 O₂ 및 CO₂ 가스농도를 측정한 결과 O₂ 농도는 12.9%, CO₂ 농도는 4.6%로 측정되어 묘삼의 저온저장 시 필름을 적용할 경우 공기의 유동이 원활이 이루어져 내부 O₂ 농도를 일정 수준 이상 유지해주는 필름을 사용하는 것이 묘삼의 출아와 생육에 좋은 것으로 조사되었다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.1
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• pp.7-13
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• 1989

The present study was carried out to get the basic information for clarifying physiological mechanism of breaking dormancy and sprouting in Panax ginseng roots. Changes in Abscisic acid (ABA) content and Gibberellin (GA) activity were investigated in one-year-old root during stratification at 4$^{\circ}C$. 15$^{\circ}C$. and 15$^{\circ}C$ after 60day-treatment at 4$^{\circ}C$. Sprouting rate at 15$^{\circ}C$ was 35％ in 30days storage at 4$^{\circ}C$ and 100％ in longer than 60days, but there was no sprout in both the constant treatment at 4$^{\circ}C$ or 15$^{\circ}C$ regardless of the treatment period. The longer the period of low temperature treatment. number of days to the first and 50％ sprouting was shortened, and number of days to 50％ from first sprouting was also shortened. ABA content in the upper part of root(contained bud) was gradually increased at both 4$^{\circ}C$ and 15$^{\circ}C$ as the treatment period was extended. and the degree of increase was higher at 15$^{\circ}C$. In the lower part. it showed a slight increase at 15$^{\circ}C$. while showed little change at 4$^{\circ}C$ throughout the treatment period. In the 15$^{\circ}C$ treatment after 60days at 4$^{\circ}C$, it was greatly increased in the upper part. while rather slightly decreased in the lower part of root. GA activity in the upper part was gradually decreased at both 4$^{\circ}C$ and 15$^{\circ}C$, and the degree of decrease was higher at 15$^{\circ}C$. In the lower part. it was similar tendency to those in the upper part. In the 15$^{\circ}C$ treatment after 60days at 4$^{\circ}C$. it was remarkably increased in both the upper and lower part. The increase was great in the low Rf region, while the decrease appeared relatively in the high Rf region compared to those of 60day-treatment at 4$^{\circ}C$. The above results indicated that the breaking dormancy and sprouting of bud were closely associated with the degree of GA activities in response to temperature condition .during stratification rather than the direct effect associated with the changes in ABA content.