• 한국산학기술학회논문지
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• 제15권8호
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• pp.5393-5399
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• 2014

미세조류 유래 mycosporine-like amino acids (MAAs) 혼합물의 항주름활성을 분석하기 위하여 type I procollagen, elastin 및 involucrin 유전자 발현분석을 실시하였다. Chlamydomonas sp.를 80% 에탄올로 추출하고, 컬럼을 통해 분획물을 정제하고, HPLC 분석을 통해 asterina 330+palythine (A+P) 및 shinorine+palythine (S+P) 혼합물을 정제하였다. MTT assay 결과 A+P 및 S+P는 0.1 mg/mL까지 세포독성을 나타내지 않았다. UV에 노출된 섬유아세포에 대한 MAAs 혼합물의 영향을 분석하였을 때, 0.05 mg/mL A+P 및 0.01 mg/mL S+P 농도에서 각각 2.7 및 3.6 배 PCOLCE mRNAs 발현이 증가하였다. Elastin 유전자는 0.01 mg/mL의 A+P 및 S+P를 처리하였을 때 각각 5.59배 및 3.1배 발현이 증가하였다. 특히 0.01 mg/mL의 A+P 및 S+P 농도에서 involucrin mRNAs 발현이 UV 처리된 대조구와 비교하였을 때 5배 및 2.5배 감소하였다. 결론적으로 Chlamydomonas sp. 유래 MAAs 혼합물은 주름개선용 기능성 화장품 개발을 위한 소재로써의 가능성이 충분한 것으로 판단되었다.

• Fisheries and Aquatic Sciences
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• 제15권4호
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• pp.361-363
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• 2012

We investigated ultraviolet (UV) absorption materials from Russian seaweeds. First, the UV absorptivities of five seaweeds Gloiopeltis fucatas, Mazzaella sp., Mastocarpus pacificus, Laminaria cichorioides, Saccharina japonica were evaluated by a UV spectrometer. Of these seaweeds, Gloiopeltis fucatas and Mazzaella sp. showed high levels of UV absorption. Column chromatography of active 50% aqueous ethanol extracts from Gloiopeltis fucatas and Mazzaella sp. resulted in the purification of two known compounds. Spectroscopic techniques identified their structures as shinorine and palythine. These materials exhibited UV absorptive capabilities at wavelengths of 333 and 320 nm, respectively. These results suggest that Gloiopeltis fucatas and Mazzaella sp. may be useful as natural cosmeceutical sources.

• 환경생물
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• 제21권4호
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• pp.368-376
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• 2003

The UV-B sensitivity was tested for the intertidal species Chondrus ocellatus from Korea, by measuring photosynthesis estimated as effective quantum yield ($\Phi_{PSII}$) of photosystem II (PS II), growth and content and composition of photosynthetic pigments and UV-absorbing pigments (UVAPs). The $$\Phi_{PSII}$ of the alga decreased with increasing time of exposure to UV-B radiation, followed by fast and nearly full recovery indicating dynamic photoinhibiton. Fresh weight-based growth and pigment contents of C. ocellatus were not seriously affected by UV-B radiation. A single broad peak at 327 nm was obtained from methanol extracts of C. ocellatus, and the absorbance peak increased with increasing UV. The single peak was resolved into three peaks (311, 330 and 336 nm) by the fourth -derivative, and quantitative change in response to UV-B radiation occurred only at 330 nm. High performance liquid chromatography (HPLC) analysis of purified extracts indicated that three MAAs (mycosporine-like amino acids) are present, asterina 330, palythine and shinorine. Field observations during three growing months showed that C. ocellatus exhibit the highest amount of UVAPs in May followed by July and little trace in September, coinciding with the species' phenology. In an ecological context, dynamic photoinhibition as well as accumulation of UVAPs may enable the shallow water red alga C. ocellatus to be well adapted to high UV-B environments.

• Ocean and Polar Research
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• 제25권4호
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• pp.427-435
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• 2003

Growth and pigment responses to different levels of solar radiation with or without ultraviolet (UV)-B component $({\lambda}=280-315nm)$ were investigated in Antarctic rhodophytes, Kallymenia antarctica and Palmaria decipiens, collected around King George Island during the summer of 2000. In K. antarctica specific growth rate, based on thallus area or fresh weight, decreased with increasing solar irradiances while P. decipiens were relatively insensitive to the effects of light. It is noticeable that the presence or absence of UV-B had no significant effect on growth for either species. However, K. antarctica showed a more pronounced reduction in chlorophyll (Chl a) concentrations at higher irradiances in the presence of UV-B. In P. decipiens, Chl a concentrations did not differ despite radiation level fluctuations being lower albeit than initial measurements. Thallus thickness was greater in K. antarctica than in P. decipiens. There were higher relative amounts of UV-absorbing pigments (UVAPs) in P. decipiens than in K, antarctica. The single absorbance peak obtained from the methanol extracts was resolved into three (316,332 and 346nm) in K. antarctica and four peaks (315,326,333 and 349 nm) in Palmaria as a result of the fourth-derivative. After 7 days exposure to solar radiation, the amount of UVAPs in K. antarctica was significantly reduced to a similar degree at all light levels, whereas that of P. decipiens remained unchanged except at 5% of surface irradiance. High performance liquid chromatography (HPLC) analysis of purified extracts indicated that P. decipiens possesses porphyra-334 in addition to three other mycosporine-like anlino acids (MAAs; asterina-330, palythine, shinorine), which are commonly present in K. antarctica. Significantly lower tolerance of K. antarctica to high levels of solar radiation may be connected with its usual absence in the eulittoral, while the active growth and elastic pigment responses of P. decipiens over a wide range of solar irradiance levels up to full sunlight seems to correspond well with its wide vertical distribution from rock pools down to 25-30m.