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http://dx.doi.org/10.5762/KAIS.2014.15.8.5393

Anti-corrugation activity of micosporine-like amino acid mixtures from Chlamydomonas sp.  

Suh, Sung-Suk (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Seo, Hyo Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
Lee, Jeong Hun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
Hwang, Jinik (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Park, Mirye (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.15, no.8, 2014 , pp. 5393-5399 More about this Journal
Abstract
To examine the effects of a mycosporine-like amino acids (MAAs) mixture from microalgae, Chlamydomonas sp, on the anti-wrinkle activities, the expression levels of genes that are associated with skin aging, including type I procollagen, elastin and involucrin, were analyzed. Asterina 330+palythine (A+P) and shinorine+palythine (S+P) mixtures were purified from Chlamydomonas sp using the following steps: 80% methanolic extraction, column purification, and HPLC analysis. As a result of the MTT assay, A+P and S+P did not induce cellular cytotoxicity with up to 0.1 mg/mL of both MAAs. In addition, the treatment of UV-exposed fibroblasts with A+P (0.05 mg/mL) and S+P (0.01 mg/mL) increased the levels of the PCOLCE mRNAs by 2.7 and 3.6 fold compared to the control group, respectively, The levels of elastin gene expression were elevated 5.59 and 3.1 fold in the A+P and S+P treated (0.01 mg/mL) cells, respectively. In particular, at a concentration of 0.01 mg /mL, the A+P and S+P expression levels of Involucrin mRNAs were increased 3.5 and 2.5 fold in the UV-exposed cells compared to the control, respectively. In conclusion, the MAAs derived from Chlamydomonas sp can be utilized as functional cosmetic materials with anti-wrinkle effects on the skin.
Keywords
Anti-corrugation; Elasin; Involucrin; Mycosporine-like amino acids; PCOLCE;
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