• Korean Journal of Veterinary Research
• /
• v.30 no.3
• /
• pp.317-331
• /
• 1990

Chromosomes of gonadal tissues from Fasciola spp, Eurytrema pancreaticum and Calicophoron calicophorum occurred Korean cattle were egamined using modified air-drying method. To compare their phenotype with three different genotypes among Fasciola spp, the adult and egg si2e were measured since they have been known as important taxonomical characters. The results obtained were as followed; Cattle liver fluke, Fasciola spp were classified into three types based on their chromosomal complements such as individual with 2o chromosome(diploid), 30 chromosome(triploid) and 20/30 mosaic constitution(mixoploid). The propotions of appearance of three types were 40.00%, 54.29% and 5.71%, respectively. The frequency of three types in type I which was regarded as F gigantica were 58.82% for diploid, 35.29% for triploid and 5.88% for mixoploid, but in type II which was regarded as F hepatica were 72.2% for triploid, 22.22% for diploid and 5.56% for mixoploid. Egg length of triploid forms was significantly larger than that of diploid forms and egg size of mixoploid forms was similiar to that of triploid forms. Worm size of triploid forms was larger than that of diploid forms and was more similar to that of mixoploid forms, but the statistical data were not significant. Diploid chromosome consisted of one pair of metacentric chromosome(m), four pairs of submetacentric chromosomes(sm), five pairs of subtelocentric chromosomes(st), while triploid chromosome consisted of one pair of metacentric chromosome, seven pairs of submetacen.tric chromosomes, one pair of subtelocentric chromosome and telocentric chromosome(t), respectively. In mixoploid chromosome, constitution of the chromosomes of diploid or triploid cell was consistent with that of diploid or triploid. Chromosomes of gonadal tissues from pancreatic fluke, Eurytrema pancreaticum consisted of 13 pairs of homologs(2n=26, n=13). The mitotic and meiotic divisions were observed frequently. In the mitotic metaphase, Karyotype consisted of five pairs of metacentric chromosomes, four pairs of submetacentric chromosomes, three pairs of subtelocentric chromosomes and one pair of telocentric chromosome. Chromosomes of gonadal tissues from stomach fluke, Calicvphoron calicophorum consisted of 9 pairs of homologs(2n=18, n=9). The meiotic divisions was frequently observed, but mitotic divisions was rare. In the mitotic metaphase, Karyotype consisted of two pairs of metacentric chromosomes, three pairs of submetacentric chromosomes and four pairs of subtelocentric chromosomes. Karyotype of Calicophoron calicophorum differed from that of Japanese C calicophorum which was similar to that of Paramphistomum cervi of Korean cattle. Though that of Calicophoron calicophorum of Korean cattle was similar to that of Paramphistomum explanatum of Korean cattle, that have been recognized to be a different species of fluke.

• Parasites, Hosts and Diseases
• /
• v.40 no.3
• /
• pp.107-112
• /
• 2002

A scanning electron microscopic study was performed on the surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adults. Metacercariae were collected from gills and muscles of mullets (Mugil cephalus) caught in a known endemic area, and adult flukes were harvested from dogs after 8 weeks of experimental infection. The worm was calabash form with its posterior part broader than the anterior part. Tegumental spines were densely distributed over the body surface, except on the suckers and genital apparatus, and around the excretory pore. Well differentiated spines were observed on the anterior half of the body, with 14-16 tips ventrally, and 19-20 tips dorsally. On the oral sucker. three pairs of type I sensory papillae (uni-ciliated knob-like swellings) and one pair of type II sensory papillae (aciliated round-swellings) were observed on the anterior and posterior parts of the lip, respectively. On the lip of the ventral sucker, one pair of type II sensory papillae was distributed only on its posterior part. Sperms were seen emerging from or entering into the genital apparatus. The results showed that the surface ultrastructure of P. summa was unique among the heterophyid trematodes, especially in digitation of tegumental spines and in distribution of sensory papillae on oral and ventral suckers.

• The Journal of Korean Academy of Prosthodontics
• /
• v.38 no.6
• /
• pp.765-781
• /
• 2000

The purpose of this study was to compare the screw loosening characteristics of three avail-able cementation type abutments: one-piece cementation type abutment; two-piece cementation type abutment using titanium abutment screw; two-piece cementation type abutment using gold abutment screw. Two implant supported three-unit superstructures were fabricated using a pair of 3 kinds of abutments for each experimental model. Cyclic loading was applied on the specimen, and made to stop when the superstructure showed movement over threshold range. The loaded cycle was counted until the machine stopped. Frequency analysis was done to measure the change of natural frequency before and after the application of cyclic load and to find the effect of screw loosening on the change of natural frequency. The specimen assembly was modeled to perform the finite element analysis to see the distribution of the stress induced by the application of preload over the screw joint and to compare the pattern of the distribution of stress induced by the external force with the change of the preload condition. The following results were obtained: 1. The failure loading cycle of two-piece cementation type abutment using gold screw was significantly greater than those of the other groups. 2. One-piece cementation type abutment applied to multi-unit restoration case did not show greater resistance to screw loosening compared to two-piece cementation type abutments. 3. Frequency analysis showed decrease in natural frequency when screw loosening occured.

• Korean Journal of Microbiology
• /
• v.54 no.4
• /
• pp.420-427
• /
• 2018

The orthogonal pair of Saccharomyces cerevisiae tyrosyl-tRNA synthetase (Sc YRS) and a variant of E. coli initiator tRNA, fMam $tRNA_{CUA}$ which recognizes the amber stop codon is an effective tool for site-specific incorporation of unnatural amino acids into the protein in E. coli. To evolve the amber suppression activity of the orthogonal pair, we generated a mutant library of Sc YRS by randomizing two amino acids at 320 and 321 which involve recognition of the first base of anticodon in fMam $tRNA_{CUA}$. Two positive clones are selected from the library screening with chloramphenicol resistance mediated by amber suppression. They showed growth resistance against high concentration of chloramphenicol and their $IC_{50}$ values were approximately 1.7~2.3 fold higher than the wild type YRS. In vivo amber suppression assay reveals that mutant YRS-3 (mYRS-3) clone containing amino acid substitutions of P320A and D321A showed 6.5-fold higher activity of amber suppression compared with the wild type. In addition, in vitro aminoacylation kinetics of mYRS-3 also showed approximately 7-fold higher activity than the wild type, and the enhancement was mainly due to the increase of tRNA binding affinity. These results demonstrate that optimization of anticodon recognition by engineered aminoacyl tRNA synthetase improves the efficiency of unnatural amino acid incorporation in response to nonsense codon.

• 제어로봇시스템학회:학술대회논문집
• /
• 1997.10a
• /
• pp.1704-1707
• /
• 1997

In this work, position and vibratiion control of a two-link manipulator with one flexible link, which an unkoun but bounded payload mass and two pair of artificial muscle-type penumatic actuators, are investgated. A flexible link robot has advantages over a figid link robot in the sense that it is much safer when it cones into contact with its environment, including humans. Furthermore, for the sake of safety, it would be more desirabel if an actuator could deliver required force while maintaining proper compliance. An artificial muscle-type penumatic actuator is adequate for such cases. In this study, a controller based on singular perturbation method, adaptive and sliding mode contro, and .mu.-synthesis is developed. The effectiveness of the proposed control scheme is confirmed through simulations and experiments.

• Proceedings of the IEEK Conference
• /
• 2003.07c
• /
• pp.2843-2846
• /
• 2003

This paper proposes a PWM(Pulse Width Modulation) circuit using CMOS OTA with a single-voltage supply. The OTA employed has an input stage which consists of a pair of two MOSFETs operating in plural operation regions. The MOSFETs work complemetarily and realize a rail-to-rail input range. The input stage requires no matching of an n-channel type input circuit and a p-channel type input circuit unlike conventional rail-to-rail input stages because the input stage is realized by single channel type MOSFETs. In order to confirm the validity of the proposed circuit, it is simulated by H-SPICE program. Futhermore, the proposed circuit will be integrated on chip using 0.35 $\mu\textrm{m}$ CMOS technology.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 1995.10a
• /
• pp.542-545
• /
• 1995

This paper provides an investigation of torque converter system using ERF (Electro-Rheological Fluid). The torque converter system using ERP is a new concepting device because we can change an apparent viscosity of ERF by adapting an electric field. The device was designed by using the equations which were proposed by Carlson et al. The devices based on ERF generally assume one two possible forms. One is the parallel plate type in which the device elements are facing circular disks separated by a flat layer of ERF, The other is coaxial cylinder or Couette types in which the ERF file the annular apace between a pair of coaxial cylindrical electrode. The discussion on this study is specifically for coaxial cylinder gemetry and experiment results show that the measured torque was rapidly increased with the increase of the eletric field.

• Journal of the Korean Graphic Arts Communication Society
• /
• v.20 no.1
• /
• pp.113-122
• /
• 2002

We designed the illumination optical system to use Fresnel lens which is the most general type of large aperture system. The normal construction of Fresnel lens is a thin molded piece of material, usually some kind of plastic, with one side flat and the other comprising a series of concentric segments each angled in a way to provide for optimum focusing for a pair of conjugate points. The 155 mm Marine Signal Lantern is the approved for use on buoys and a maximum omnidirectional range of 8 nmi, can be obtained with standard 12V equipment, respectively. Finally, Fresnel lens with 155 mm diameter was designed and evaluated. Also, we re-designed groove "out" of Fresnel lens and compared results of groove "in" type with groove "out" type. According to the results, Fresnel lens is obtained best optical results when grooved side faces the longer conjugate.de faces the longer conjugate.

• Proceedings of the KIEE Conference
• /
• 1989.07a
• /
• pp.142-145
• /
• 1989

The electromagnetic suspension system, which is a kind of magnetic levitation, can be categorized into two groups; separate lift & guidance system and combined lift & guidance system. This paper deals with the latter system, in which lift and guidance forces are generated by a pair of staggered magnets with the inverted U- shaped rail. In this work, a rigid body bogie-truck and a twist response type of bogie-truck, which are constructed by two magnetic wheels consist of two staggered magnet pairs, are modeled, and curvature running characteristics of both types obtained by simulation are presented. Simulation result showed that curvature running characteristics of twist response type of bogie-truck is better than that of rigid body bogie-truck.

• Proceedings of the KSME Conference
• /
• 2008.11a
• /
• pp.1437-1440
• /
• 2008

This research describes about designing and manufacturing X-wing type flapping micro aerial vehicle which intends to improve the performance of one-pair wing flapping vehicle with innovated design. This design, X-wing as we call, was introduced for some time ago from many laboratories but still there hasn’t any reports dealing on its theoretical or numerical analysis. By manufacturing the X-wing with our own design and succeeding its flight test will give us the general idea on X-wing which may guide us to conduct the numerical and experimental analysis later on. We focused to design the X-wing and introduce some conceptual theories about its characteristics on this report.