• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.289-297
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• 2021

Bacillus velezensis CJ1, showing significant fibrinolytic activity, was isolated from Myeolchi Jeotgal, a popular Korean fermented seafood. When B. velezensis CJ1 was grown on four different culture media, the culture on the Luria-Bertani (LB) broth showed the highest fibrinolytic activity (102.94 mU/μl) at 48 h. LB was also the best medium for growth. SDS-PAGE of culture supernatant showed four major bands, 38, 35, 27, and 22 kDa in size. Fibrin zymography showed four active bands, 50, 47, 40, and 30 kDa in size. A gene homologous to aprE of the Bacillus species was cloned by PCR. DNA sequencing showed that aprECJ1 can encode a protease consisting of 382 amino acids. The translated amino acid sequence of AprECJ1 showed high identity values with those of B. velezensis strains and other Bacillus species. The aprECJ1 gene was introduced into B. subtilis WB600 using an E. coli-Bacillus shuttle vector, pHY300PLK, and overexpressed. A 27 kDa band corresponding to the mature form of AprECJ1 was produced and confirmed by SDS-PAGE and fibrin zymography. B. subtilis WB600 [pHYaprECJ1] showed 1.8-fold higher fibrinolytic activity than B. velezensis CJ1 at 48 h.

• Journal of the Korean Society of Costume
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• v.51 no.3
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• pp.129-139
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• 2001

The purpose of this study is to suggest the standard size specification of the apparel item as finding out apparel products'sizing problem in the domestic and foreign interned shopping malls. Though that, to suggest basic data of web page offered sizing system given to consumer higher satisfaction and based on ISO (International Organization For Standardization), domestic industry standard and service related size. The summarized findings are as follow. 1. The result of size description research, the size description system between internet site were very different, in addition in the department shopping malls it was different by manufacturing industry. Also in domestic and foreign shopping malls, It was not correspond to ISO and domestic industry standard, used Numeric and Alphabetical size. In the foreign shopping mall it distinguish body type and age group. 2. The result of consumer's satisfaction, the superior fashion malls were Samsung, LG, Fashionpia in the domestic shopping mall, were Gap, JCPenny, Jcrew in the foreign shopping malls. In the total group, 1-5 grades were ranked in the foreign shopping malls. 3. Satisfaction of the foreign fashion mall was higher than that of the domestic fashion malls. Exactly, foreign fashion mall described size chart, size description, product size and body size measuring method but domestic fashion malls didn't describe that. Domestic fashion malls need sizing system development given to consumer higher satisfaction. In addition sizing system based on ISO and domestic industry standard and subdivided to body type and ale group must be studied. Aloe, fashion information, coordination information and product explanation must be reinforced.

• Journal of the Optical Society of Korea
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• v.6 no.4
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• pp.172-179
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• 2002

Like the conventional digital storage systems, volume holographic memory can be deteriorated by burst errors due to its high-density storage characteristics. These burst errors are used byoptical defects such as scratches, dust particles, etc. and are two-dimensional in a data page. To deal with these errors, we introduce some concepts for describing them and propose efficient two- dimensional interleaving schemes. The schemes are two-dimensional lattices of an error-correction code word and have equilateral triangular and square structures. Using these structures, we can minimize the number of code words that are interleaved and improve the efficiency of the system. For large size burst errors, the efficient interleaving structure is an equilateral triangular lattice. However, for some small size burst errors, it is reduced to a square lattice.

• Journal of Fisheries and Marine Sciences Education
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• v.17 no.1
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• pp.67-75
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• 2005

Mobile devices compared with personal computers on the desktop have low bandwidths, small screens, and relatively slow speeds. These systems have practical problems with information searches in a web environment. Information searching of various web contents on the small size screen has especially severe limitations. We propose a filtering technique of web contents which can overcome the limitations of small size screens and meet the user requirements in a PDA environment. For these constructions, we first divide the screen into segment blocks and then extract content blocks according to the user requirements, so that only filtered web contents will be shown. The performance evaluation of the proposed technique saves an average time of about 30% by displaying only the extracted information instead of the whole web page.

• Journal of Species Research
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• v.5 no.3
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• pp.381-384
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• 2016

Two marine amoebae Vannella bursella (Page, 1974) Smirnov et al., 2007 and Pseudoparamoeba sp. were encountered from marine coastal waters of Masan Bay and Garorim Bay (Korea), respectively. These species are described with uninterpreted records based on light-microscopy of living cells and reported taxonomically for the first time from Korea. Diagnostics of these species are as follows. Vannella bursella: size in vivo, $17-29{\mu}m$ long with flattened ovoid, semicircular locomotive forms. Pseudoparamoeba sp.: size in vivo, $10-15{\mu}m$ long with elongated locomotive forms, producing a few short conical pseudopodia from anterior hyaline zone.

• BMB Reports
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• v.28 no.1
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• pp.46-50
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• 1995

Three kinds of mouse tumor necrosis factor (TNF), which have molecular weights of 35 kDa, 45 kDa, and 18 kDa on SDS-PAGE, were partially purified from serum-free culture supernatants of mouse peritoneal macrophages induced with lipopolysaccharide. Analysis of the native molecular weights by gel filtration indicated that the 18 kDa and 45 kDa TNFs aggregate into 50 kDa and 100 kDa molecules, respectively, while the 35 kDa TNF is contained in high molecular weight aggregates of approximately 200 kDa. The three kinds of cytotoxic factors all elicited tumor reducing responses.

• Journal of the Korea Society of Computer and Information
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• v.21 no.2
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• pp.1-7
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• 2016

In this paper, we propose in this paper present a novel locality data allocation policy as COLD(Correlated Locality Data) allocation policy. COLD is defined as a set of data that will be updated together later. By distributing a COLD into a NAND block separately, it can preserve th locality. In addition, by handling multiple COLD simultaneously, it can obtain the parallelism among NAND chips. We perform two experiment to demonstrate the effectiveness of the COLD data allocation policy. First, we implement COLD detector, and then, analyze a well-known workload. And we confirm the amount of COLD found depending on the size of data constituting the COLD. Secondly, we compared the traditional page-level mapping policy and COLD for garbage collection overhead in actual development board Cosmos OpenSSD. Experimental results have shown that COLD data allocation policy is significantly reduces the garbage collection overhead. Also, we confirmed that garbage collection overhead vary depending on the COLD size.

• Journal of Life Science
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• v.25 no.6
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• pp.631-637
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• 2015

Streptavidin, a protein produced by Streptomyces avidinii, strongly binds up to four molecules of vitamin H, d-biotin exhibiting the dissociation constant of about 10⁻¹⁵ M. This strong binding affinity has been applied for detection and characterization of numerous biological molecules suggesting expression and purification of functional streptavidin should be very useful for the application of this streptavidin-biotin interaction. To express a soluble streptavidin in Escherichia coli, We synthesized streptavidin genes and cloned into pET-22b plasmid, which uses T7 RNA polymerase/T7 promoter expression systems containing pelB leader for secretion into periplasmic space and six polyhistidine tags at C-terminus for purification of expressed proteins. Although streptavidin is toxic to Escherichia coli due to strong biotin binding property, streptavidin was expressed very sufficiently in a range of 10-20 mg/ml. In SDS-PAGE, the size of purified protein was shown as 17 kDa in denatured condition (boiling) and 68 kDa in native condition (without boiling) suggesting tetramerization of monomeric subunit by non-covalent association. Further analysis by size-exclusion chromatography supported streptavidin’s tetrameric structure as well. In addition, soluble streptavidin detected biotinylated proteins in westernblot indicating its functional activity to biotin. Taken these results together, it concluded that our simple expression system was able to show high yield, homotetrameric formation and biotin binding activity analogous to natural streptavidin.

• Biomedical Science Letters
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• v.12 no.3
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• pp.177-183
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• 2006

To demonstrate the effect of formulation conditions and evaluations of structural integrity from ovalbumin containing poly lactide glycolide copolymer (PLGA) microspheres for Vaccine delivery, OVA microspheres were prepared by a W/O/W multiple emulsion solvent extraction technique. Dichloromethan (DCM) and Ethyl acetate (EA) were applied as an organic phase and poly vinyl alcohol (PVA) as a secondary emulsion stabilizer. Microspheres were characterized for particle size, morphology (optical microscopy and Scanning Electron Microscope (SEM)). Protein denaturation was evaluated by size exclusion chromatography (SEC), SDS-PAGE and isoelectric focusing (IEF). Residual organic solvent was estimated by gas chromatography (GC) and differential scanning calorimetry (DSC). Optical photomicrograph and SEM revealed that micro spheres were typically spherical but various morphologies were observed. Mean particle size $(d_{vs})$ of microspheres were in the range of $3{\sim}50{\mu}m$. Also, The protein stability was not affected by the fonnulation process and residual organic solvent was beyond the detection below 0.1ppm. These results demonstrated that micro spheres might be a good candidate for the parenteral vaccine delivery system.

• The Journal of the Korea Contents Association
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• v.16 no.10
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• pp.477-482
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• 2016

This paper presents a new method of parity storing for ECC(error correcting code) in SSD (solid-state drive) and suitable structure of the controller. In general usage of NAND flash memory, we partition a page into data and spare area. ECC parity is stored in the spare area. The method has overhead on area and timing due to access of the page memory discontinuously. This paper proposes a new parity policy storing method that reduces overhead and R(read)/W(write) timing by using whole page area continuously without partitioning. We analyzed overhead and R/W timing. As a result, the proposed parity storing has 13.6% less read access time than the conventional parity policy with 16KB page size. For 4GB video file transfer, it has about a minute less than the conventional parity policy. It will enhance the system performance because the read operation is key function in SSD.